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The map label for this gene is ilvE [H]

Identifier: 220905536

GI number: 220905536

Start: 2739210

End: 2740172

Strand: Direct

Name: ilvE [H]

Synonym: Ddes_2275

Alternate gene names: 220905536

Gene position: 2739210-2740172 (Clockwise)

Preceding gene: 220905535

Following gene: 220905537

Centisome position: 95.33

GC content: 61.89

Gene sequence:

>963_bases
TTGAAAGCAGTCGATGCCCATACCTATCTTGCGGCCCTGCTGGCCGCTCCCCGGCCCGGTTCTGAAAACGTGCTGGCGTT
CTATGATCACAGGGTGGGGCATATCTGCACTGACGCGGCCCTTCTGCTTTTGCCGCTGGATGATCACATCTGCCACAGGG
GCGACGGTCTTTTTGAGAGCATCAGCTACCGCCAGGGCAGGCTGTTCAGCTTTGACCAGCACCTTGCGCGCCTCAAGGAC
GGAGCCGCGGCTCTCGGCATCCCCCCTCCCTGTCCGTGGGATACACTGCGCCGCATCATACTGGACGTGGCCCGCGCCTC
GGGCAGCGATCACGGCGACATGCGCATATTCCTGAGCCGAGGCCCTGGCGGCTTCGGCATCAGCCCGGCTGAATGCCCCC
AGGCAGGCCTGTATGTGGTGGCCCTGCGCAAAAAATTTGCGGGCCAAGCCTTTTATGAAAAAGGGCTCACCGCCTTTACC
AGTGACATCCCGCCCAAGCAGGAATACCTGGCCCGCATAAAAAACACCAATTATCTGCCCAATGTGTTCATGGCTATGGA
GGCCGCCCGCAAGGGCATGGACGTTGCCGTGACCTTTGATGAAGACGGCTTCATGGGCGAAGCCGCCACAGCCAACGTGG
GCCTTGTGGACGAACGGGGACGCCTGCTCTGCCCCGAACTCAGACGCATCCTGCCCGGCACCACCATGCTGGCCGCCCTT
GAACTGGCCGCGCGCCGCCACCCCGGTCCCATGACGGTAATAGAGGCCCCCATAGCCAAAGAACAGATCAGCACGGCCAG
TGAAATGCTGCTTTTCACCAGTTCCACCCTCTGCGTGGGGGTGACCCATTTTGACGGAAAGCCCGTGGGCTGCGGCGAGC
ATCTGGGCAGGCCGGGTCCGGTGGCCCGGTGGCTCAAGGATGCCCTGCTGCGGCATTTGATGGAACAAGGTACTCCCTTC
TGA

Upstream 100 bases:

>100_bases
TGCGCGTGACGGACAAAAACGTCTTTATGTCCTGATATTGAACGCATGCAGCAAACGTGCGGGGCGTTCTGCGCCCCGCG
CGCCTTTTGAGGAGACGCCC

Downstream 100 bases:

>100_bases
TGCGTGTTCTGCTTATTGCCCTGCAAAATACGGTTCCCGGCCCCGCCTCGCCGGAAGAGCAGGCCCGGTGGGCCGAACTC
GGCTCGCCCATGCGCACCGC

Product: class IV aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKAVDAHTYLAALLAAPRPGSENVLAFYDHRVGHICTDAALLLLPLDDHICHRGDGLFESISYRQGRLFSFDQHLARLKD
GAAALGIPPPCPWDTLRRIILDVARASGSDHGDMRIFLSRGPGGFGISPAECPQAGLYVVALRKKFAGQAFYEKGLTAFT
SDIPPKQEYLARIKNTNYLPNVFMAMEAARKGMDVAVTFDEDGFMGEAATANVGLVDERGRLLCPELRRILPGTTMLAAL
ELAARRHPGPMTVIEAPIAKEQISTASEMLLFTSSTLCVGVTHFDGKPVGCGEHLGRPGPVARWLKDALLRHLMEQGTPF

Sequences:

>Translated_320_residues
MKAVDAHTYLAALLAAPRPGSENVLAFYDHRVGHICTDAALLLLPLDDHICHRGDGLFESISYRQGRLFSFDQHLARLKD
GAAALGIPPPCPWDTLRRIILDVARASGSDHGDMRIFLSRGPGGFGISPAECPQAGLYVVALRKKFAGQAFYEKGLTAFT
SDIPPKQEYLARIKNTNYLPNVFMAMEAARKGMDVAVTFDEDGFMGEAATANVGLVDERGRLLCPELRRILPGTTMLAAL
ELAARRHPGPMTVIEAPIAKEQISTASEMLLFTSSTLCVGVTHFDGKPVGCGEHLGRPGPVARWLKDALLRHLMEQGTPF
>Mature_320_residues
MKAVDAHTYLAALLAAPRPGSENVLAFYDHRVGHICTDAALLLLPLDDHICHRGDGLFESISYRQGRLFSFDQHLARLKD
GAAALGIPPPCPWDTLRRIILDVARASGSDHGDMRIFLSRGPGGFGISPAECPQAGLYVVALRKKFAGQAFYEKGLTAFT
SDIPPKQEYLARIKNTNYLPNVFMAMEAARKGMDVAVTFDEDGFMGEAATANVGLVDERGRLLCPELRRILPGTTMLAAL
ELAARRHPGPMTVIEAPIAKEQISTASEMLLFTSSTLCVGVTHFDGKPVGCGEHLGRPGPVARWLKDALLRHLMEQGTPF

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=270, Percent_Identity=25.5555555555556, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34775; Mature: 34775

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVDAHTYLAALLAAPRPGSENVLAFYDHRVGHICTDAALLLLPLDDHICHRGDGLFES
CCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHCCEEEEEECCHHHHHCCCCHHHH
ISYRQGRLFSFDQHLARLKDGAAALGIPPPCPWDTLRRIILDVARASGSDHGDMRIFLSR
HHCCCCCCCHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEC
GPGGFGISPAECPQAGLYVVALRKKFAGQAFYEKGLTAFTSDIPPKQEYLARIKNTNYLP
CCCCCCCCHHCCCCCCEEEEEHHHHHCCHHHHHHCCHHHHCCCCCHHHHHHHHCCCCCCH
NVFMAMEAARKGMDVAVTFDEDGFMGEAATANVGLVDERGRLLCPELRRILPGTTMLAAL
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEECCCCCEECHHHHHHCCCHHHHHHH
ELAARRHPGPMTVIEAPIAKEQISTASEMLLFTSSTLCVGVTHFDGKPVGCGEHLGRPGP
HHHHHCCCCCCEEEECCHHHHHHHHHHHHHHEECCEEEEEEEECCCCCCCCHHHCCCCCH
VARWLKDALLRHLMEQGTPF
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKAVDAHTYLAALLAAPRPGSENVLAFYDHRVGHICTDAALLLLPLDDHICHRGDGLFES
CCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHCCEEEEEECCHHHHHCCCCHHHH
ISYRQGRLFSFDQHLARLKDGAAALGIPPPCPWDTLRRIILDVARASGSDHGDMRIFLSR
HHCCCCCCCHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEC
GPGGFGISPAECPQAGLYVVALRKKFAGQAFYEKGLTAFTSDIPPKQEYLARIKNTNYLP
CCCCCCCCHHCCCCCCEEEEEHHHHHCCHHHHHHCCHHHHCCCCCHHHHHHHHCCCCCCH
NVFMAMEAARKGMDVAVTFDEDGFMGEAATANVGLVDERGRLLCPELRRILPGTTMLAAL
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEECCCCCEECHHHHHHCCCHHHHHHH
ELAARRHPGPMTVIEAPIAKEQISTASEMLLFTSSTLCVGVTHFDGKPVGCGEHLGRPGP
HHHHHCCCCCCEEEECCHHHHHHHHHHHHHHEECCEEEEEEEECCCCCCCCHHHCCCCCH
VARWLKDALLRHLMEQGTPF
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]