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The map label for this gene is cooH [H]

Identifier: 220905147

GI number: 220905147

Start: 2273450

End: 2274532

Strand: Direct

Name: cooH [H]

Synonym: Ddes_1885

Alternate gene names: 220905147

Gene position: 2273450-2274532 (Clockwise)

Preceding gene: 220905146

Following gene: 220905148

Centisome position: 79.12

GC content: 59.83

Gene sequence:

>1083_bases
ATGACCAGCACCTTCACCATGCCTCTCGGGCCGGTACACGTGGCCCTTGAGGAACCGGTATACTTCCACCTGACGGTGGA
CGGTGAAACCGTGCGTCACGTGGAACTGACCTCCGGCCATGTGCACCGCGGCATGGAGGCCATGGCCACCCAGCGCAACC
TGGTCAAAAACGTCACGCTTACCGAACGCGTGTGCTCCCTGTGCTCCAACAGCCACTCCTTCACCTACAGCATGGTGGTG
GAAAACGTGCTGGGCATCACCATCCCCGACCGCGCGCGCTATTTGCGCGTAGTGGCGGAAGAAATCAAGCGTATCGCCTC
GCACCTGTTCAACACTGCCATTCAGGCGCACATCATCGGCTTCAAGTCGCTGTTCATGCACGTCATGGAAGTGCGTGAAA
TGATGCAGGACCTGAAAGAAACCGTTTACGGCAACCGCATGAACCTGGCTGCGAACTGCATTGGCGGCGTAAAATACAAT
GTCAATGCGGAGCTGCTGGACTACATGCGCAAAATGCTGGACAAGGTGGAGCCGCAGGTGGACGAAATCCGCGAGATTTA
CGACACCAACAGCATGGTTCTGGCCCGTACCAAGGGCCTTGGCGTGCTGCCCAAGGAAGACGCCATCCGCCTTGGCGTGG
TTGGCCCTGTGGCGCGCGGCTCCGGTCTGCAGCTGGACGTGCGCAAGGACTCGCCTTACGCCGCCTATCCTGATGTGGAA
TTTAAAACCATTGTGGAACAGAGCGGCTGCATCCACGCCCGCACTATGGTGCGCATGCACGAAGTGCTCGAATCCTTCAG
CATCATCCGCCAGTGCATCGCGCGCATGCCCGAAGGCGAGGTAACGGCCCCCATGCGCCAGATACGCACGGCCGAGGCCT
GCGCCCGCTCTGAAGCGCCGCGCGGCGAGGTTTTCTACTATATCCGCACCAATGAGACGGACATACCCTCGCGCCTCAAG
TGGCGTGTGCCTTCCTACATGAACTGGGAGGCTCTGGGCGTGATGATGCGTGACTGCAAGGTGGCCGATGTGGCCCTGAT
CACCAACAGTATCGACCCCTGCGTGTCCTGCACGGAACGCTAG

Upstream 100 bases:

>100_bases
CGGGCATTCTCAACGAAGCCGTGCCTCTTTCCATCATGATGAACGGCGCCTGTACCACCGACCTGTGGGAACGCATCCTC
AAGGATAAGGAGAACCAGTC

Downstream 100 bases:

>100_bases
GGCAGTACGGCGATGCATGCCGGATGCTGCCGGAAGAACGGATTTTTCTGCGCGGAACGCCCCTGCGGCGTTCCGCCGGA
TAAAAACCGGCCCGGCGCAC

Product: NADH dehydrogenase (quinone)

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTSTFTMPLGPVHVALEEPVYFHLTVDGETVRHVELTSGHVHRGMEAMATQRNLVKNVTLTERVCSLCSNSHSFTYSMVV
ENVLGITIPDRARYLRVVAEEIKRIASHLFNTAIQAHIIGFKSLFMHVMEVREMMQDLKETVYGNRMNLAANCIGGVKYN
VNAELLDYMRKMLDKVEPQVDEIREIYDTNSMVLARTKGLGVLPKEDAIRLGVVGPVARGSGLQLDVRKDSPYAAYPDVE
FKTIVEQSGCIHARTMVRMHEVLESFSIIRQCIARMPEGEVTAPMRQIRTAEACARSEAPRGEVFYYIRTNETDIPSRLK
WRVPSYMNWEALGVMMRDCKVADVALITNSIDPCVSCTER

Sequences:

>Translated_360_residues
MTSTFTMPLGPVHVALEEPVYFHLTVDGETVRHVELTSGHVHRGMEAMATQRNLVKNVTLTERVCSLCSNSHSFTYSMVV
ENVLGITIPDRARYLRVVAEEIKRIASHLFNTAIQAHIIGFKSLFMHVMEVREMMQDLKETVYGNRMNLAANCIGGVKYN
VNAELLDYMRKMLDKVEPQVDEIREIYDTNSMVLARTKGLGVLPKEDAIRLGVVGPVARGSGLQLDVRKDSPYAAYPDVE
FKTIVEQSGCIHARTMVRMHEVLESFSIIRQCIARMPEGEVTAPMRQIRTAEACARSEAPRGEVFYYIRTNETDIPSRLK
WRVPSYMNWEALGVMMRDCKVADVALITNSIDPCVSCTER
>Mature_359_residues
TSTFTMPLGPVHVALEEPVYFHLTVDGETVRHVELTSGHVHRGMEAMATQRNLVKNVTLTERVCSLCSNSHSFTYSMVVE
NVLGITIPDRARYLRVVAEEIKRIASHLFNTAIQAHIIGFKSLFMHVMEVREMMQDLKETVYGNRMNLAANCIGGVKYNV
NAELLDYMRKMLDKVEPQVDEIREIYDTNSMVLARTKGLGVLPKEDAIRLGVVGPVARGSGLQLDVRKDSPYAAYPDVEF
KTIVEQSGCIHARTMVRMHEVLESFSIIRQCIARMPEGEVTAPMRQIRTAEACARSEAPRGEVFYYIRTNETDIPSRLKW
RVPSYMNWEALGVMMRDCKVADVALITNSIDPCVSCTER

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=380, Percent_Identity=28.6842105263158, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI260898743, Length=378, Percent_Identity=28.5714285714286, Blast_Score=153, Evalue=2e-37,
Organism=Escherichia coli, GI1789076, Length=356, Percent_Identity=33.9887640449438, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788832, Length=356, Percent_Identity=33.4269662921348, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI145693162, Length=382, Percent_Identity=26.7015706806283, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17555284, Length=382, Percent_Identity=26.9633507853403, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17568379, Length=380, Percent_Identity=27.1052631578947, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24638644, Length=377, Percent_Identity=29.1777188328912, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI221459469, Length=373, Percent_Identity=27.3458445040214, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 40664; Mature: 40533

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTFTMPLGPVHVALEEPVYFHLTVDGETVRHVELTSGHVHRGMEAMATQRNLVKNVTL
CCCEEECCCCCEEEEECCCEEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCHH
TERVCSLCSNSHSFTYSMVVENVLGITIPDRARYLRVVAEEIKRIASHLFNTAIQAHIIG
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKSLFMHVMEVREMMQDLKETVYGNRMNLAANCIGGVKYNVNAELLDYMRKMLDKVEPQV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCEEECCCHHHHHHHHHHHHHHCCCH
DEIREIYDTNSMVLARTKGLGVLPKEDAIRLGVVGPVARGSGLQLDVRKDSPYAAYPDVE
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCH
FKTIVEQSGCIHARTMVRMHEVLESFSIIRQCIARMPEGEVTAPMRQIRTAEACARSEAP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
RGEVFYYIRTNETDIPSRLKWRVPSYMNWEALGVMMRDCKVADVALITNSIDPCVSCTER
CCCEEEEEECCCCCCCCHHEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
TSTFTMPLGPVHVALEEPVYFHLTVDGETVRHVELTSGHVHRGMEAMATQRNLVKNVTL
CCEEECCCCCEEEEECCCEEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCHH
TERVCSLCSNSHSFTYSMVVENVLGITIPDRARYLRVVAEEIKRIASHLFNTAIQAHIIG
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKSLFMHVMEVREMMQDLKETVYGNRMNLAANCIGGVKYNVNAELLDYMRKMLDKVEPQV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCEEECCCHHHHHHHHHHHHHHCCCH
DEIREIYDTNSMVLARTKGLGVLPKEDAIRLGVVGPVARGSGLQLDVRKDSPYAAYPDVE
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCH
FKTIVEQSGCIHARTMVRMHEVLESFSIIRQCIARMPEGEVTAPMRQIRTAEACARSEAP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
RGEVFYYIRTNETDIPSRLKWRVPSYMNWEALGVMMRDCKVADVALITNSIDPCVSCTER
CCCEEEEEECCCCCCCCHHEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]