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The map label for this gene is hisA

Identifier: 220905074

GI number: 220905074

Start: 2185446

End: 2186198

Strand: Direct

Name: hisA

Synonym: Ddes_1811

Alternate gene names: 220905074

Gene position: 2185446-2186198 (Clockwise)

Preceding gene: 220905073

Following gene: 220905075

Centisome position: 76.06

GC content: 61.22

Gene sequence:

>753_bases
ATGATTATTTTTCCCGCTGTTGACATTCAGAACGGCAAGGCCGTGCGGCTTAAGCAGGGCCGCGCTCACGAAAGCACGGT
TTTTGCCGAAAGCCCCGTAGATGCCGCCAGAGCCTGGGAAGAAGCCGGAGCGCGCTGGCTGCATGTGGTTGACCTGGACG
GCGCTTTTGACGGTGCGGCCCAAAGCCGCCGCATCGTGCGCGACATCTGCGCGGCTCTTGCCATTCCTGTACAGCTTGGC
GGCGGCATACGCGACATGCATACGGCCGAAGCCTATCTGGAAGCCGGAGTCAGCCGCCTTATTATCGGCACCTTGGCTCT
GGAGCAGCCCACCCTGTTTGCGGACATGTGCCGCGCCTTTCCCGGCCGGGTAGGCGTTTCTCTCGATGCCGAAGGCGGAA
GGCTGAAAAGCCGCGGCTGGGTGGAAGACACGGGCATGACCGTGGATACGGCCTTGCCCCGCCTGCTTGACGATGGGGCT
GCGTTTGTCATCTATACAGATATTGAACGCGACGGCATGCAGTGCGGCGTCAACGTAGCCGCTCTGGAGCATCTGGCTCG
TCTTTCCACCGTGCCGGTCATTGCCGCCGGGGGCGTCGCCACCCTTGAAGATATACAGAAACTCTACCCTCTCACCCTTG
AAACCAGCCTTATGGGCGCGGTGAGCGGGCGTGCCCTCTACGAGGGAACCCTTGATCTCAAAGAAGCCAACGCCTGGATA
GACGCCCGGAAAAACACGCCCGAAAGCGCCTGA

Upstream 100 bases:

>100_bases
TGCTCAACGTGGGTTCATTCCTCAAGCGCAAGGGGCAGTGGGTCACTGCCGAGGAACTGACCGTGGACGGCATAAGCAAG
GCAGTAAAGGACTTGGGTCT

Downstream 100 bases:

>100_bases
CCAGACCGCTCGAACAGTTCACCAAGACAGTCAAAACAACGCAAAGAGCGCGTCACGGTTCAGTCCGGACGCGCTCTTTG
CGTACACAGGCACAGCGCAG

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MIIFPAVDIQNGKAVRLKQGRAHESTVFAESPVDAARAWEEAGARWLHVVDLDGAFDGAAQSRRIVRDICAALAIPVQLG
GGIRDMHTAEAYLEAGVSRLIIGTLALEQPTLFADMCRAFPGRVGVSLDAEGGRLKSRGWVEDTGMTVDTALPRLLDDGA
AFVIYTDIERDGMQCGVNVAALEHLARLSTVPVIAAGGVATLEDIQKLYPLTLETSLMGAVSGRALYEGTLDLKEANAWI
DARKNTPESA

Sequences:

>Translated_250_residues
MIIFPAVDIQNGKAVRLKQGRAHESTVFAESPVDAARAWEEAGARWLHVVDLDGAFDGAAQSRRIVRDICAALAIPVQLG
GGIRDMHTAEAYLEAGVSRLIIGTLALEQPTLFADMCRAFPGRVGVSLDAEGGRLKSRGWVEDTGMTVDTALPRLLDDGA
AFVIYTDIERDGMQCGVNVAALEHLARLSTVPVIAAGGVATLEDIQKLYPLTLETSLMGAVSGRALYEGTLDLKEANAWI
DARKNTPESA
>Mature_250_residues
MIIFPAVDIQNGKAVRLKQGRAHESTVFAESPVDAARAWEEAGARWLHVVDLDGAFDGAAQSRRIVRDICAALAIPVQLG
GGIRDMHTAEAYLEAGVSRLIIGTLALEQPTLFADMCRAFPGRVGVSLDAEGGRLKSRGWVEDTGMTVDTALPRLLDDGA
AFVIYTDIERDGMQCGVNVAALEHLARLSTVPVIAAGGVATLEDIQKLYPLTLETSLMGAVSGRALYEGTLDLKEANAWI
DARKNTPESA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=253, Percent_Identity=33.201581027668, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788336, Length=249, Percent_Identity=24.4979919678715, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_DESDA (B8J249)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480386.1
- ProteinModelPortal:   B8J249
- GeneID:   7285524
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1811
- HOGENOM:   HBG541613
- ProtClustDB:   PRK00748
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26684; Mature: 26684

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIFPAVDIQNGKAVRLKQGRAHESTVFAESPVDAARAWEEAGARWLHVVDLDGAFDGAA
CEEECEEECCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHCCCEEEEEEEECCCCCCHH
QSRRIVRDICAALAIPVQLGGGIRDMHTAEAYLEAGVSRLIIGTLALEQPTLFADMCRAF
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
PGRVGVSLDAEGGRLKSRGWVEDTGMTVDTALPRLLDDGAAFVIYTDIERDGMQCGVNVA
CCCCCEEECCCCCEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHCCCHHH
ALEHLARLSTVPVIAAGGVATLEDIQKLYPLTLETSLMGAVSGRALYEGTLDLKEANAWI
HHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCHHHCCCEE
DARKNTPESA
ECCCCCCCCC
>Mature Secondary Structure
MIIFPAVDIQNGKAVRLKQGRAHESTVFAESPVDAARAWEEAGARWLHVVDLDGAFDGAA
CEEECEEECCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHCCCEEEEEEEECCCCCCHH
QSRRIVRDICAALAIPVQLGGGIRDMHTAEAYLEAGVSRLIIGTLALEQPTLFADMCRAF
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
PGRVGVSLDAEGGRLKSRGWVEDTGMTVDTALPRLLDDGAAFVIYTDIERDGMQCGVNVA
CCCCCEEECCCCCEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHCCCHHH
ALEHLARLSTVPVIAAGGVATLEDIQKLYPLTLETSLMGAVSGRALYEGTLDLKEANAWI
HHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCHHHCCCEE
DARKNTPESA
ECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA