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The map label for this gene is purR [H]

Identifier: 220905044

GI number: 220905044

Start: 2150206

End: 2151321

Strand: Reverse

Name: purR [H]

Synonym: Ddes_1781

Alternate gene names: 220905044

Gene position: 2151321-2150206 (Counterclockwise)

Preceding gene: 220905047

Following gene: 220905043

Centisome position: 74.87

GC content: 58.06

Gene sequence:

>1116_bases
ATGACGATTGATGGCGGAAAGCAGGCCGAAACGGGCACAAAAAAGGCAACCCTTGCGGATGTGGCCCAGGCGGCGGAGGT
GTCACCCGCCACGGTTTCAATGATTCTCAATGGCCGTGAAGGAGTTTCTTTTGCCGAGGATACAGTCAAGACGGTTCTGG
CCGCGGCAGCAAAGCTCAACTACACCGGCGGCAGACGCGGCAGGGTACCCCCGGGAAGCAGGCCCGTCATACTGATTGCC
GTGCCGAACGTAACGAACCCTTATTACGCCACGATTATTCAGGCCATTCAGCAGGCAGCGGAAAAAAAGAATTACGCCAC
CTGCGTAAGCGCCACCTACCGCAGCCTGGAAAATGAGCTGGCGGCCTTGCAGCTGGCCCGTTCCACAGGCATGGCCGGTA
TTATATTCGCCATGCTGGCGCACCCTGACGCCATACTGGCGCGCGCCGGGCGCAAGATGCCCCTGGTGGTTATGGGCGAC
CGGCGCGTGGGCCTTAATGTAGATACGGTAGAGCTGAACAACTACGATGCGGGCAGTCTTATTGCCCAGCACATGTGCGA
ACTGGGGCATAAGCACATGGCCTATATTTCCACATCGCTGGACACCTGTAACCCCATCAGGGTGCAGCGCCACAAGGGCC
TGCAGGACACCTTGGCCCGGCTCTGCCCCGAAGGCCGCCTTGTGGTCAAAAGCCGCGATATTTCGCCTGAAACGGAACTG
GAAAACCTGCTTATCGAGCACACCGTGGGCTACGAACTGGCCCGGGGCTGTTTTGACGACAAAAAGATCACGGCCTTTGT
GGCCGTCAACGATATGGTGGCCTACGGGGTCATGGATGCCGTGATTGAAGCCGGTTTGAGCATCCCCGATGATTACAGCG
TCTGCGGATTCGACAATCTCTGGCCCTCGCATTTTGCCCGTGTGTCTTTGACGACCATCGAGCATTATATCAATGACAAG
GGGCATAATGCCCTGGACCTGCTGCATGCCAGAATCAGCGGGCAGCTCTCGAACCGGAATATAACCAGGGTCGAGGTCAG
CCACAAACTTATTGCGCGCGCCTCTACCGGCGCTCCGCGTCGATCTTGGGCCGGACCTTCCGGCTCAGGATCGTAA

Upstream 100 bases:

>100_bases
CGAAATGCCGGCATTGTGTGTCCCGCGTATTGCAGGTAAGAATGCTTCAGGCGTTATGCGTCCGTACAAACAGGATATGA
ATGCCGGAAGGAAAGAAAAG

Downstream 100 bases:

>100_bases
CGGCCTGTCTGCCTATGCATAGCCATTTGACTCGTCAGCAACTGGAAGCCCTTACCCTGCGTTGGGAACAGTGGGAAAGC
GAGGCTGCCACCAGGGCGCA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTIDGGKQAETGTKKATLADVAQAAEVSPATVSMILNGREGVSFAEDTVKTVLAAAAKLNYTGGRRGRVPPGSRPVILIA
VPNVTNPYYATIIQAIQQAAEKKNYATCVSATYRSLENELAALQLARSTGMAGIIFAMLAHPDAILARAGRKMPLVVMGD
RRVGLNVDTVELNNYDAGSLIAQHMCELGHKHMAYISTSLDTCNPIRVQRHKGLQDTLARLCPEGRLVVKSRDISPETEL
ENLLIEHTVGYELARGCFDDKKITAFVAVNDMVAYGVMDAVIEAGLSIPDDYSVCGFDNLWPSHFARVSLTTIEHYINDK
GHNALDLLHARISGQLSNRNITRVEVSHKLIARASTGAPRRSWAGPSGSGS

Sequences:

>Translated_371_residues
MTIDGGKQAETGTKKATLADVAQAAEVSPATVSMILNGREGVSFAEDTVKTVLAAAAKLNYTGGRRGRVPPGSRPVILIA
VPNVTNPYYATIIQAIQQAAEKKNYATCVSATYRSLENELAALQLARSTGMAGIIFAMLAHPDAILARAGRKMPLVVMGD
RRVGLNVDTVELNNYDAGSLIAQHMCELGHKHMAYISTSLDTCNPIRVQRHKGLQDTLARLCPEGRLVVKSRDISPETEL
ENLLIEHTVGYELARGCFDDKKITAFVAVNDMVAYGVMDAVIEAGLSIPDDYSVCGFDNLWPSHFARVSLTTIEHYINDK
GHNALDLLHARISGQLSNRNITRVEVSHKLIARASTGAPRRSWAGPSGSGS
>Mature_370_residues
TIDGGKQAETGTKKATLADVAQAAEVSPATVSMILNGREGVSFAEDTVKTVLAAAAKLNYTGGRRGRVPPGSRPVILIAV
PNVTNPYYATIIQAIQQAAEKKNYATCVSATYRSLENELAALQLARSTGMAGIIFAMLAHPDAILARAGRKMPLVVMGDR
RVGLNVDTVELNNYDAGSLIAQHMCELGHKHMAYISTSLDTCNPIRVQRHKGLQDTLARLCPEGRLVVKSRDISPETELE
NLLIEHTVGYELARGCFDDKKITAFVAVNDMVAYGVMDAVIEAGLSIPDDYSVCGFDNLWPSHFARVSLTTIEHYINDKG
HNALDLLHARISGQLSNRNITRVEVSHKLIARASTGAPRRSWAGPSGSGS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=358, Percent_Identity=26.8156424581006, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789202, Length=307, Percent_Identity=27.0358306188925, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790194, Length=348, Percent_Identity=25.2873563218391, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790369, Length=333, Percent_Identity=27.027027027027, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788474, Length=328, Percent_Identity=25, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787580, Length=338, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI48994940, Length=289, Percent_Identity=24.5674740484429, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787906, Length=211, Percent_Identity=27.4881516587678, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39949; Mature: 39818

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDGGKQAETGTKKATLADVAQAAEVSPATVSMILNGREGVSFAEDTVKTVLAAAAKLN
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
YTGGRRGRVPPGSRPVILIAVPNVTNPYYATIIQAIQQAAEKKNYATCVSATYRSLENEL
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AALQLARSTGMAGIIFAMLAHPDAILARAGRKMPLVVMGDRRVGLNVDTVELNNYDAGSL
HHHHHHHHCCHHHHHHHHHHCCHHHHHHCCCCCCEEEECCCEECCCEEEEEECCCCHHHH
IAQHMCELGHKHMAYISTSLDTCNPIRVQRHKGLQDTLARLCPEGRLVVKSRDISPETEL
HHHHHHHHCCHHEEHEECCCCCCCCCHHHHCCCHHHHHHHHCCCCEEEEEECCCCCHHHH
ENLLIEHTVGYELARGCFDDKKITAFVAVNDMVAYGVMDAVIEAGLSIPDDYSVCGFDNL
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
WPSHFARVSLTTIEHYINDKGHNALDLLHARISGQLSNRNITRVEVSHKLIARASTGAPR
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCCCCC
RSWAGPSGSGS
CCCCCCCCCCC
>Mature Secondary Structure 
TIDGGKQAETGTKKATLADVAQAAEVSPATVSMILNGREGVSFAEDTVKTVLAAAAKLN
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
YTGGRRGRVPPGSRPVILIAVPNVTNPYYATIIQAIQQAAEKKNYATCVSATYRSLENEL
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AALQLARSTGMAGIIFAMLAHPDAILARAGRKMPLVVMGDRRVGLNVDTVELNNYDAGSL
HHHHHHHHCCHHHHHHHHHHCCHHHHHHCCCCCCEEEECCCEECCCEEEEEECCCCHHHH
IAQHMCELGHKHMAYISTSLDTCNPIRVQRHKGLQDTLARLCPEGRLVVKSRDISPETEL
HHHHHHHHCCHHEEHEECCCCCCCCCHHHHCCCHHHHHHHHCCCCEEEEEECCCCCHHHH
ENLLIEHTVGYELARGCFDDKKITAFVAVNDMVAYGVMDAVIEAGLSIPDDYSVCGFDNL
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
WPSHFARVSLTTIEHYINDKGHNALDLLHARISGQLSNRNITRVEVSHKLIARASTGAPR
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCCCCC
RSWAGPSGSGS
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA