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The map label for this gene is gluQ [H]

Identifier: 220905032

GI number: 220905032

Start: 2137606

End: 2138601

Strand: Direct

Name: gluQ [H]

Synonym: Ddes_1769

Alternate gene names: 220905032

Gene position: 2137606-2138601 (Clockwise)

Preceding gene: 220905031

Following gene: 220905033

Centisome position: 74.39

GC content: 63.15

Gene sequence:

>996_bases
ATGCATATTTCTGAACGTCCCATGCAGCATTCATCCATCCCTTGCGGCAGGCTGGCCCCAAGCCCCACCGGGCATATCCA
CCTGGGCAACGCCTGGGCCTTTTTGCTGGCTTGGCTTGCCGCCCGCTCACAGGGTGGACAGGTGCTGCTGCGCCTTGAAG
ATATCGACCCGCAGCGCTCCCGGCCGGACTATGCCGCAGCCATACTGGAAGACCTGCGCTGGCTCGGCCTTGACTGGGAC
TATGGGCCGGATACTGGCGGCCCCGGCGGCCCGTATGTGCAGAGCCTGCGCAGCGAGATTTACGACACGGCGCTGACGCA
GCTTGAACAGTCCGGACTGACCTATCCCTGTTTCTGCACCCGCAAGGAATTGCGCCAGCTTGCCGCCGCGCCGCATGTGG
ACGATACAGGCGCACCCTATCCCGGTACCTGCCGTCATCTGAACGAAGAACAGATTCAGGCCATGCTGCAATCGGGGCGC
AAAGCCTGCATGCGTCTGCGCTGTCCCCAAACGCCCATACGCTTTGACGATGCCGTCATGGGACCACAGAGCTTTCTTCT
TTCCCGGTGCGGAGGCGACTTTGCCCTGCGCCGTTCCGACGGTGTTGTGGCCTACCAGCTGGCTGTAGCCGTGGACGACG
CCCTTATGGGGGTCACCCAGGTGGTGCGTGGACGGGACATTCTGCCCTCGACCCCGCGCCAGATTGCTCTTCTCGAACTC
TTGGGCTATGGTGCGCCTTCCTATGCCCATGTGCCCCTGCTTCTGGATGCAGAGGGAGAGCGCCTGGCAAAGCGCCACAA
CAGCCTTTCCCTGCGCTGCCTGCGGGAACAGCGGGTTGATCCGCGCCGCATCACAGGTTTGCTGGGTCACCTGGCTGGCC
TTACTTTTTCCGCCGCAAGCGCAAGCCCGGCGGAGCTTATGCCCGCTTTTGACTGGAGCCGCCTGCCACGACAGGATGTG
CGCCTTGAACACAGGCACCTGCGCGGGCTGCTGTGA

Upstream 100 bases:

>100_bases
GGCCACCTGATGCCCCGGGGGCGGGGCAAGTGCGGCCATGATGTTCCGGTTCCGGCACAACTGCTGCCCTGCGTCAGTAT
AAAGATCAAGCCGGCGCGGC

Downstream 100 bases:

>100_bases
CAAAACCGGCACGGGTGAAATCGCGTAACCGGGCGGCCCCCACGATGGCCGCTGAAAACCGCGCTCCCGGTCTCTTTTTA
ACGACAGCATATCTCACAGC

Product: glutamyl-tRNA synthetase class Ic

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MHISERPMQHSSIPCGRLAPSPTGHIHLGNAWAFLLAWLAARSQGGQVLLRLEDIDPQRSRPDYAAAILEDLRWLGLDWD
YGPDTGGPGGPYVQSLRSEIYDTALTQLEQSGLTYPCFCTRKELRQLAAAPHVDDTGAPYPGTCRHLNEEQIQAMLQSGR
KACMRLRCPQTPIRFDDAVMGPQSFLLSRCGGDFALRRSDGVVAYQLAVAVDDALMGVTQVVRGRDILPSTPRQIALLEL
LGYGAPSYAHVPLLLDAEGERLAKRHNSLSLRCLREQRVDPRRITGLLGHLAGLTFSAASASPAELMPAFDWSRLPRQDV
RLEHRHLRGLL

Sequences:

>Translated_331_residues
MHISERPMQHSSIPCGRLAPSPTGHIHLGNAWAFLLAWLAARSQGGQVLLRLEDIDPQRSRPDYAAAILEDLRWLGLDWD
YGPDTGGPGGPYVQSLRSEIYDTALTQLEQSGLTYPCFCTRKELRQLAAAPHVDDTGAPYPGTCRHLNEEQIQAMLQSGR
KACMRLRCPQTPIRFDDAVMGPQSFLLSRCGGDFALRRSDGVVAYQLAVAVDDALMGVTQVVRGRDILPSTPRQIALLEL
LGYGAPSYAHVPLLLDAEGERLAKRHNSLSLRCLREQRVDPRRITGLLGHLAGLTFSAASASPAELMPAFDWSRLPRQDV
RLEHRHLRGLL
>Mature_331_residues
MHISERPMQHSSIPCGRLAPSPTGHIHLGNAWAFLLAWLAARSQGGQVLLRLEDIDPQRSRPDYAAAILEDLRWLGLDWD
YGPDTGGPGGPYVQSLRSEIYDTALTQLEQSGLTYPCFCTRKELRQLAAAPHVDDTGAPYPGTCRHLNEEQIQAMLQSGR
KACMRLRCPQTPIRFDDAVMGPQSFLLSRCGGDFALRRSDGVVAYQLAVAVDDALMGVTQVVRGRDILPSTPRQIALLEL
LGYGAPSYAHVPLLLDAEGERLAKRHNSLSLRCLREQRVDPRRITGLLGHLAGLTFSAASASPAELMPAFDWSRLPRQDV
RLEHRHLRGLL

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=300, Percent_Identity=34, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI1788743, Length=282, Percent_Identity=35.8156028368794, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI226510925, Length=257, Percent_Identity=37.3540856031128, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI71993394, Length=311, Percent_Identity=32.1543408360129, Blast_Score=153, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6324540, Length=286, Percent_Identity=36.7132867132867, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24665321, Length=277, Percent_Identity=36.8231046931408, Blast_Score=191, Evalue=4e-49,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17

Molecular weight: Translated: 36593; Mature: 36593

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHISERPMQHSSIPCGRLAPSPTGHIHLGNAWAFLLAWLAARSQGGQVLLRLEDIDPQRS
CCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
RPDYAAAILEDLRWLGLDWDYGPDTGGPGGPYVQSLRSEIYDTALTQLEQSGLTYPCFCT
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
RKELRQLAAAPHVDDTGAPYPGTCRHLNEEQIQAMLQSGRKACMRLRCPQTPIRFDDAVM
HHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHC
GPQSFLLSRCGGDFALRRSDGVVAYQLAVAVDDALMGVTQVVRGRDILPSTPRQIALLEL
CHHHHHHHHCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
LGYGAPSYAHVPLLLDAEGERLAKRHNSLSLRCLREQRVDPRRITGLLGHLAGLTFSAAS
HHCCCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCEECCCC
ASPAELMPAFDWSRLPRQDVRLEHRHLRGLL
CCHHHHCCCCCHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHISERPMQHSSIPCGRLAPSPTGHIHLGNAWAFLLAWLAARSQGGQVLLRLEDIDPQRS
CCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
RPDYAAAILEDLRWLGLDWDYGPDTGGPGGPYVQSLRSEIYDTALTQLEQSGLTYPCFCT
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
RKELRQLAAAPHVDDTGAPYPGTCRHLNEEQIQAMLQSGRKACMRLRCPQTPIRFDDAVM
HHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHC
GPQSFLLSRCGGDFALRRSDGVVAYQLAVAVDDALMGVTQVVRGRDILPSTPRQIALLEL
CHHHHHHHHCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
LGYGAPSYAHVPLLLDAEGERLAKRHNSLSLRCLREQRVDPRRITGLLGHLAGLTFSAAS
HHCCCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCEECCCC
ASPAELMPAFDWSRLPRQDVRLEHRHLRGLL
CCHHHHCCCCCHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA