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The map label for this gene is yafL [H]

Identifier: 220905031

GI number: 220905031

Start: 2136309

End: 2137286

Strand: Direct

Name: yafL [H]

Synonym: Ddes_1768

Alternate gene names: 220905031

Gene position: 2136309-2137286 (Clockwise)

Preceding gene: 220905030

Following gene: 220905032

Centisome position: 74.35

GC content: 57.67

Gene sequence:

>978_bases
ATGGGAAAATGTCTGAAACTGTGCGCGTTGATGATGGGCTGCGTGTTGGCCTTTGGTTGCGCCGCCAAAAAAGGTCCACA
GGAAGACAGCATTGACAGCCTGCGCGCCGAGCGGTTCCGACGCTCCTATGAGGCGGCTTTTGATTCCAACCAGCAAGAGG
CCGGCCAGCAGCTCCTTCGCAAGGCCAGGTCAGCCATCGGCACCCCCTATGTGCGCGGCGGAACTACCCCGGACGGCTTT
GACTGTTCGGGTTTTGTCTGCTGGACGTATAAAAGCGTCGGCGTACAGTTGCCCCGCACCGCGCGTGAACAGTCTGTCAT
CGGGCAGCGCATCACCAAGGTGGAAGACATGCGTGCCGGAGATATCGTGGCCTTTCGCCACCCCCGTCGGGGCTATCATA
CAGGCATCTATGTGGGCGACGGCAAGTTTATCCACAGCCCACGCAAACGCACCAAGGTGCGCATCAATTCTCTGGACGAT
CCCTATTTCAAAACCACGTTTCTCAGCGCGCGCCGCGTAAAAGTTGAAGGCGGCGAAACGCTGACGGCTCAGGCCGAAAG
CCGCCTCAGCGATTTTACCGAAGAAAAGGTCATACGCGAACTGGCCCGCGACAAGAAAAAACCAGCAAAAAGCCGGGCCG
CTGCCACACGCGACAACAAGCGCACGAACACGCGAGAGAAAAAACGGGATGCGACCATACAGGTCGCCAGCAACGACAGA
AAGGGCGCCGCCCCGGCCCGCGCTGCAGAAGCCAGAAACAGCAAAAATGCCAAGCAGGCCGCGCAGACCGCCAAAGCGCC
AGCCAAAACTGCCGGCAAAGCCCAAAGCAAATCACAGAACAAGGCCGGAGCAACAGCCAAAAAACCCGACAATAAAAAAA
CCGAAGCCAAAAAGCCCGCACCCGCCAAAACCTCGGCAGCCAAAAGTGACTCCGGGAACAAGAAGAATCAGGGCAAGGAC
AAAAACAACAGATCCTGA

Upstream 100 bases:

>100_bases
AGACACCGGGGCCGGAAGCCCGACAGTTTACGCCGGGCATGGCCCGTTTTCCGACTGACCTGCCGCCACCTTCTGGCCGG
CATAACATTGTGGAGCGCTG

Downstream 100 bases:

>100_bases
CGCGATCAGGGCCGGTCCGGCAGCAATTGACAGCAAGCCGATGCCGGTTCCGGCAAGGCCGGACGGCAGCCGGGTAAAAC
CGGGCAACGGCGGATGCCCG

Product: NLP/P60 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MGKCLKLCALMMGCVLAFGCAAKKGPQEDSIDSLRAERFRRSYEAAFDSNQQEAGQQLLRKARSAIGTPYVRGGTTPDGF
DCSGFVCWTYKSVGVQLPRTAREQSVIGQRITKVEDMRAGDIVAFRHPRRGYHTGIYVGDGKFIHSPRKRTKVRINSLDD
PYFKTTFLSARRVKVEGGETLTAQAESRLSDFTEEKVIRELARDKKKPAKSRAAATRDNKRTNTREKKRDATIQVASNDR
KGAAPARAAEARNSKNAKQAAQTAKAPAKTAGKAQSKSQNKAGATAKKPDNKKTEAKKPAPAKTSAAKSDSGNKKNQGKD
KNNRS

Sequences:

>Translated_325_residues
MGKCLKLCALMMGCVLAFGCAAKKGPQEDSIDSLRAERFRRSYEAAFDSNQQEAGQQLLRKARSAIGTPYVRGGTTPDGF
DCSGFVCWTYKSVGVQLPRTAREQSVIGQRITKVEDMRAGDIVAFRHPRRGYHTGIYVGDGKFIHSPRKRTKVRINSLDD
PYFKTTFLSARRVKVEGGETLTAQAESRLSDFTEEKVIRELARDKKKPAKSRAAATRDNKRTNTREKKRDATIQVASNDR
KGAAPARAAEARNSKNAKQAAQTAKAPAKTAGKAQSKSQNKAGATAKKPDNKKTEAKKPAPAKTSAAKSDSGNKKNQGKD
KNNRS
>Mature_324_residues
GKCLKLCALMMGCVLAFGCAAKKGPQEDSIDSLRAERFRRSYEAAFDSNQQEAGQQLLRKARSAIGTPYVRGGTTPDGFD
CSGFVCWTYKSVGVQLPRTAREQSVIGQRITKVEDMRAGDIVAFRHPRRGYHTGIYVGDGKFIHSPRKRTKVRINSLDDP
YFKTTFLSARRVKVEGGETLTAQAESRLSDFTEEKVIRELARDKKKPAKSRAAATRDNKRTNTREKKRDATIQVASNDRK
GAAPARAAEARNSKNAKQAAQTAKAPAKTAGKAQSKSQNKAGATAKKPDNKKTEAKKPAPAKTSAAKSDSGNKKNQGKDK
NNRS

Specific function: Unknown

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1786421, Length=124, Percent_Identity=44.3548387096774, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787944, Length=128, Percent_Identity=41.40625, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788501, Length=108, Percent_Identity=40.7407407407407, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788001, Length=172, Percent_Identity=34.3023255813954, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 35554; Mature: 35422

Theoretical pI: Translated: 11.01; Mature: 11.01

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKCLKLCALMMGCVLAFGCAAKKGPQEDSIDSLRAERFRRSYEAAFDSNQQEAGQQLLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KARSAIGTPYVRGGTTPDGFDCSGFVCWTYKSVGVQLPRTAREQSVIGQRITKVEDMRAG
HHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
DIVAFRHPRRGYHTGIYVGDGKFIHSPRKRTKVRINSLDDPYFKTTFLSARRVKVEGGET
CEEEEECCCCCCCCCEEECCCCEECCCCHHHEEEECCCCCCHHHHHHHHHEEEEECCCCE
LTAQAESRLSDFTEEKVIRELARDKKKPAKSRAAATRDNKRTNTREKKRDATIQVASNDR
EHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHCCCEEEEECCCC
KGAAPARAAEARNSKNAKQAAQTAKAPAKTAGKAQSKSQNKAGATAKKPDNKKTEAKKPA
CCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PAKTSAAKSDSGNKKNQGKDKNNRS
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GKCLKLCALMMGCVLAFGCAAKKGPQEDSIDSLRAERFRRSYEAAFDSNQQEAGQQLLR
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KARSAIGTPYVRGGTTPDGFDCSGFVCWTYKSVGVQLPRTAREQSVIGQRITKVEDMRAG
HHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
DIVAFRHPRRGYHTGIYVGDGKFIHSPRKRTKVRINSLDDPYFKTTFLSARRVKVEGGET
CEEEEECCCCCCCCCEEECCCCEECCCCHHHEEEECCCCCCHHHHHHHHHEEEEECCCCE
LTAQAESRLSDFTEEKVIRELARDKKKPAKSRAAATRDNKRTNTREKKRDATIQVASNDR
EHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHCCCEEEEECCCC
KGAAPARAAEARNSKNAKQAAQTAKAPAKTAGKAQSKSQNKAGATAKKPDNKKTEAKKPA
CCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PAKTSAAKSDSGNKKNQGKDKNNRS
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]