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The map label for this gene is ilvA [H]

Identifier: 220905026

GI number: 220905026

Start: 2131924

End: 2133462

Strand: Reverse

Name: ilvA [H]

Synonym: Ddes_1763

Alternate gene names: 220905026

Gene position: 2133462-2131924 (Counterclockwise)

Preceding gene: 220905036

Following gene: 220905020

Centisome position: 74.25

GC content: 60.62

Gene sequence:

>1539_bases
ATGCACAAGCACAGCGAATACCTGAACCGCATTTTGTTGAGCCGCGTTTATGACGTGGCTGTGGAGACGCCCCTCGAAGA
AGCCAAGACCCTTTCGCGTCGCCTCGGCAACCGAATTCTGCTCAAGCGCGAGGATTATCAGCCTGTTTTTTCCTTCAAGC
TGCGTGGCGCGTACAACAAGATGGCCCGCCTTTCGCCGGAAGACCTGCGGCGCGGCGTTATTGCCGCCTCGGCGGGCAAC
CATGCCCAGGGCGTAGCCCTGGCGGCGCGCAAGCTCGGCTGCGAAGCTACCATCGTCATGCCTGTGACGACCCCCGCCAT
CAAGGTGGAGGCTGTGCGCCGTCTGGGCGGGCAGGTGGTGCTTTCAGGCGAATCGTTCAGCGATGCCTGGCAGCATACGC
TTAAACTTATTCAGGAAAGCGGCTGTATCTATGTACCGCCTTTTGACGATCCCGATGTCATTGCCGGGCAGGGAACCATC
GCCATGGAAATTCTGCGGCAACACCCCGGCGATATACACGCTGTTTTTGTTCCCATCGGCGGCGGGGGCATGGCGGCGGG
CGTTGCGGCCTATATCAAGGCCTTGCGGCCAGAAATCAGGGTCATCGGCGTGGAGCCTGTGGATTCCGACGCCATGCGCC
GTTCGGTCATGGCCGGACACCGGGTGGAAATGCACGACGTGGGGCTTTTTGCCGATGGCGTTGCCGTGAAGCTGGTGGGC
GAACACACTTTTGCCCTGTGCCGGGACCTCCTGGACGACATCATTGTGGTGGAGACAGACGCCATCTGCGGGGCCATAAA
GGACATCTTTGAAGATACTCGCCTGGTTGCCGAACCAGCGGGAGCTTTGGCCCTGGCCGGGTTGCGCGCCTACGCTGCGG
CCAGCGGCCAGCGGGACGCCACCCTGGTGGCAGTGGTCAGCGGGGCCAACATGAACTTCGACCGTCTGAGCCATGTGGTG
GACCGGTCTGAAATCGGCGCACACCGCGAAGCCCTGCTGGCAGTGACCATTCCGGAGGCCGTGGGCAGCTTTCGCGCCCT
GTGCGCTTCTTTTGGCAGCCGCAATATTACGGAGCTTTGTACCCGTTTTTCCGACCCGGTGAAGGCGCGTGTGCTGGTGG
GCATAAAGATCAGCGGGCGGGAAGATGTGGCCGAAGTGCTGGCAGAACTGCACAGCAAGGGCTTTGGAGCCGTTGATCTG
ACAGATAACGAGTTTGCCAAGGTTCACTTGCGGCATCTGGTGGGCGGCAACGCGCCGCAGGTGCTGCATGAGCGGTTGCT
GCGCTTTACCTTTCCCGAACGGCCCGGCGCTTTGCTGGACTTTATGGACGCCATGCGCGTGGATTTCAGTATCAGCCTGT
TCCAGTACCGTTATCACGGCGCAGACTTTGGCCGCGTACTGGTGGGCTTTGAGGTTCCGGAAGAAAAGCGCGGGGTTTTC
GAGGAGTTTTTGCAGCGGGTTGAAGGCATGGGCTACCCGCATGTGGAAGAAACGGGCAATCCCGCCTACCAGATGTTTCT
GGGATGGCACGACGACTAG

Upstream 100 bases:

>100_bases
GAGCGGACGCCGGGTATATAAGAATCTGCGCTGACGAACAAGCCTTTTTCTACACGGCTGCAGGACGGCCGTGTCATTTT
TGCAGCCGAAGGACACATGC

Downstream 100 bases:

>100_bases
CACATTTTACCTTTGAAAAAAGTTACATGCGCTCATGCTGCACGAGAGTGCAGCGCGTTGTAACACGTTGTGCATTCTGT
AGAAGATTATATTTTTAGCA

Product: threonine dehydratase, biosynthetic

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MHKHSEYLNRILLSRVYDVAVETPLEEAKTLSRRLGNRILLKREDYQPVFSFKLRGAYNKMARLSPEDLRRGVIAASAGN
HAQGVALAARKLGCEATIVMPVTTPAIKVEAVRRLGGQVVLSGESFSDAWQHTLKLIQESGCIYVPPFDDPDVIAGQGTI
AMEILRQHPGDIHAVFVPIGGGGMAAGVAAYIKALRPEIRVIGVEPVDSDAMRRSVMAGHRVEMHDVGLFADGVAVKLVG
EHTFALCRDLLDDIIVVETDAICGAIKDIFEDTRLVAEPAGALALAGLRAYAAASGQRDATLVAVVSGANMNFDRLSHVV
DRSEIGAHREALLAVTIPEAVGSFRALCASFGSRNITELCTRFSDPVKARVLVGIKISGREDVAEVLAELHSKGFGAVDL
TDNEFAKVHLRHLVGGNAPQVLHERLLRFTFPERPGALLDFMDAMRVDFSISLFQYRYHGADFGRVLVGFEVPEEKRGVF
EEFLQRVEGMGYPHVEETGNPAYQMFLGWHDD

Sequences:

>Translated_512_residues
MHKHSEYLNRILLSRVYDVAVETPLEEAKTLSRRLGNRILLKREDYQPVFSFKLRGAYNKMARLSPEDLRRGVIAASAGN
HAQGVALAARKLGCEATIVMPVTTPAIKVEAVRRLGGQVVLSGESFSDAWQHTLKLIQESGCIYVPPFDDPDVIAGQGTI
AMEILRQHPGDIHAVFVPIGGGGMAAGVAAYIKALRPEIRVIGVEPVDSDAMRRSVMAGHRVEMHDVGLFADGVAVKLVG
EHTFALCRDLLDDIIVVETDAICGAIKDIFEDTRLVAEPAGALALAGLRAYAAASGQRDATLVAVVSGANMNFDRLSHVV
DRSEIGAHREALLAVTIPEAVGSFRALCASFGSRNITELCTRFSDPVKARVLVGIKISGREDVAEVLAELHSKGFGAVDL
TDNEFAKVHLRHLVGGNAPQVLHERLLRFTFPERPGALLDFMDAMRVDFSISLFQYRYHGADFGRVLVGFEVPEEKRGVF
EEFLQRVEGMGYPHVEETGNPAYQMFLGWHDD
>Mature_512_residues
MHKHSEYLNRILLSRVYDVAVETPLEEAKTLSRRLGNRILLKREDYQPVFSFKLRGAYNKMARLSPEDLRRGVIAASAGN
HAQGVALAARKLGCEATIVMPVTTPAIKVEAVRRLGGQVVLSGESFSDAWQHTLKLIQESGCIYVPPFDDPDVIAGQGTI
AMEILRQHPGDIHAVFVPIGGGGMAAGVAAYIKALRPEIRVIGVEPVDSDAMRRSVMAGHRVEMHDVGLFADGVAVKLVG
EHTFALCRDLLDDIIVVETDAICGAIKDIFEDTRLVAEPAGALALAGLRAYAAASGQRDATLVAVVSGANMNFDRLSHVV
DRSEIGAHREALLAVTIPEAVGSFRALCASFGSRNITELCTRFSDPVKARVLVGIKISGREDVAEVLAELHSKGFGAVDL
TDNEFAKVHLRHLVGGNAPQVLHERLLRFTFPERPGALLDFMDAMRVDFSISLFQYRYHGADFGRVLVGFEVPEEKRGVF
EEFLQRVEGMGYPHVEETGNPAYQMFLGWHDD

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=315, Percent_Identity=33.6507936507937, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI33469958, Length=312, Percent_Identity=27.8846153846154, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI19923959, Length=310, Percent_Identity=27.741935483871, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1790207, Length=504, Percent_Identity=51.7857142857143, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1789505, Length=308, Percent_Identity=38.961038961039, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71991565, Length=300, Percent_Identity=33.6666666666667, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17537387, Length=375, Percent_Identity=33.3333333333333, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17508781, Length=294, Percent_Identity=31.6326530612245, Blast_Score=134, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320930, Length=519, Percent_Identity=46.4354527938343, Blast_Score=450, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322631, Length=314, Percent_Identity=36.624203821656, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319788, Length=292, Percent_Identity=27.7397260273973, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355833, Length=327, Percent_Identity=32.1100917431193, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24645328, Length=250, Percent_Identity=29.6, Blast_Score=89, Evalue=9e-18,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 55941; Mature: 55941

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKHSEYLNRILLSRVYDVAVETPLEEAKTLSRRLGNRILLKREDYQPVFSFKLRGAYNK
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCHHHH
MARLSPEDLRRGVIAASAGNHAQGVALAARKLGCEATIVMPVTTPAIKVEAVRRLGGQVV
HHCCCHHHHHCCEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEE
LSGESFSDAWQHTLKLIQESGCIYVPPFDDPDVIAGQGTIAMEILRQHPGDIHAVFVPIG
ECCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEECCCHHHHHHHHHCCCCEEEEEEEEC
GGGMAAGVAAYIKALRPEIRVIGVEPVDSDAMRRSVMAGHRVEMHDVGLFADGVAVKLVG
CCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCEEECCEEEEEEC
EHTFALCRDLLDDIIVVETDAICGAIKDIFEDTRLVAEPAGALALAGLRAYAAASGQRDA
HHHHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
TLVAVVSGANMNFDRLSHVVDRSEIGAHREALLAVTIPEAVGSFRALCASFGSRNITELC
EEEEEEECCCCCHHHHHHHHHHHHCCCCHHEEEEEECHHHHHHHHHHHHHHCCCCHHHHH
TRFSDPVKARVLVGIKISGREDVAEVLAELHSKGFGAVDLTDNEFAKVHLRHLVGGNAPQ
HHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCHH
VLHERLLRFTFPERPGALLDFMDAMRVDFSISLFQYRYHGADFGRVLVGFEVPEEKRGVF
HHHHHHHHHCCCCCCCHHHHHHHHHHHCEEEEEEEEEECCCCCCEEEEEEECCHHHHHHH
EEFLQRVEGMGYPHVEETGNPAYQMFLGWHDD
HHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MHKHSEYLNRILLSRVYDVAVETPLEEAKTLSRRLGNRILLKREDYQPVFSFKLRGAYNK
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCHHHH
MARLSPEDLRRGVIAASAGNHAQGVALAARKLGCEATIVMPVTTPAIKVEAVRRLGGQVV
HHCCCHHHHHCCEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEE
LSGESFSDAWQHTLKLIQESGCIYVPPFDDPDVIAGQGTIAMEILRQHPGDIHAVFVPIG
ECCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEECCCHHHHHHHHHCCCCEEEEEEEEC
GGGMAAGVAAYIKALRPEIRVIGVEPVDSDAMRRSVMAGHRVEMHDVGLFADGVAVKLVG
CCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCEEECCEEEEEEC
EHTFALCRDLLDDIIVVETDAICGAIKDIFEDTRLVAEPAGALALAGLRAYAAASGQRDA
HHHHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
TLVAVVSGANMNFDRLSHVVDRSEIGAHREALLAVTIPEAVGSFRALCASFGSRNITELC
EEEEEEECCCCCHHHHHHHHHHHHCCCCHHEEEEEECHHHHHHHHHHHHHHCCCCHHHHH
TRFSDPVKARVLVGIKISGREDVAEVLAELHSKGFGAVDLTDNEFAKVHLRHLVGGNAPQ
HHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCHH
VLHERLLRFTFPERPGALLDFMDAMRVDFSISLFQYRYHGADFGRVLVGFEVPEEKRGVF
HHHHHHHHHCCCCCCCHHHHHHHHHHHCEEEEEEEEEECCCCCCEEEEEEECCHHHHHHH
EEFLQRVEGMGYPHVEETGNPAYQMFLGWHDD
HHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA