Click here to switch to the map view.

The map label for this gene is tcyB [H]

Identifier: 220905022

GI number: 220905022

Start: 2126099

End: 2126791

Strand: Direct

Name: tcyB [H]

Synonym: Ddes_1759

Alternate gene names: 220905022

Gene position: 2126099-2126791 (Clockwise)

Preceding gene: 220905021

Following gene: 220905023

Centisome position: 73.99

GC content: 59.16

Gene sequence:

>693_bases
ATGGACTCACTTTCAGTAGTTTACCACGCCCTGCCGTCCATGCTGGCCGGCTGCATAGTCACGGTGGGCAACGTGACCAT
TTCTCTCGCCATGGGCCTTGTGCTCGGCGTTCCTATGGCTGTGGCCCAGGTTTACGGCGGCCCCTGGCTGCGCCGCCTGG
TCGCGGTCTATGTGTGGTTTTTCCGGGGAGTGCCCATTCTTGTGCTGCTCTTTCTGTGCTACGGGCTTTTCATCAGCCTC
GGCCTGCCGGCAGACCCGTTTCTTATATGCTGCCTGGTGCTTGGCTGCACCAGTACGGCCTACCAGTCGCAAATTTTCCG
GGGAGCCATTGAAAGTCTGCCGCAGGGGCAGCTCAACGCGGCACGCGCTCTGGGCATGGGTGAAGGCACGGGTATCTGCA
GCATCATACTGCCCCAGGCCATGCGCTTGTCCATCCCCGGCTGGGCCAACGAATTTTCCATTCTGCTCAAAGATTCGGCC
ATCTGCTACGTTCTCGGCACACAGGATATCATGGCAAGAACGTCTTTTGTGGCCGCACGCACGCACGAACATCTGGCCCT
TTACGCGGCAGCGGGCTGCATTTATTTTCTGCTGACACTGGCGGTGCTGAAGCTCCTGCGCCGCCTGGAAAAAAGAGTTC
ATGTACCGGGATACTCCACGGGCATGGGCATGGAAGGCACGGGTGCGGGATAA

Upstream 100 bases:

>100_bases
GAATAAATAAGCAACGCGAAAGCGCTGAACGGCAGGTGCGGACCGCCGGTTATTCCGGCGGTCCGCGCTGTTATGCCCAA
CCTACGGCAAAACCCCGCAT

Downstream 100 bases:

>100_bases
GCCATGAATGTGGACGAACAAGCGATTCTGCGTGTTGAGGGTATCTCCAAGAAGCTGGGTGGCAAGCCCATTCTGGACAA
TTGCAGTCTTTCGGTGCGCC

Product: polar amino acid ABC transporter inner membrane subunit

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MDSLSVVYHALPSMLAGCIVTVGNVTISLAMGLVLGVPMAVAQVYGGPWLRRLVAVYVWFFRGVPILVLLFLCYGLFISL
GLPADPFLICCLVLGCTSTAYQSQIFRGAIESLPQGQLNAARALGMGEGTGICSIILPQAMRLSIPGWANEFSILLKDSA
ICYVLGTQDIMARTSFVAARTHEHLALYAAAGCIYFLLTLAVLKLLRRLEKRVHVPGYSTGMGMEGTGAG

Sequences:

>Translated_230_residues
MDSLSVVYHALPSMLAGCIVTVGNVTISLAMGLVLGVPMAVAQVYGGPWLRRLVAVYVWFFRGVPILVLLFLCYGLFISL
GLPADPFLICCLVLGCTSTAYQSQIFRGAIESLPQGQLNAARALGMGEGTGICSIILPQAMRLSIPGWANEFSILLKDSA
ICYVLGTQDIMARTSFVAARTHEHLALYAAAGCIYFLLTLAVLKLLRRLEKRVHVPGYSTGMGMEGTGAG
>Mature_230_residues
MDSLSVVYHALPSMLAGCIVTVGNVTISLAMGLVLGVPMAVAQVYGGPWLRRLVAVYVWFFRGVPILVLLFLCYGLFISL
GLPADPFLICCLVLGCTSTAYQSQIFRGAIESLPQGQLNAARALGMGEGTGICSIILPQAMRLSIPGWANEFSILLKDSA
ICYVLGTQDIMARTSFVAARTHEHLALYAAAGCIYFLLTLAVLKLLRRLEKRVHVPGYSTGMGMEGTGAG

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787030, Length=214, Percent_Identity=31.3084112149533, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788226, Length=217, Percent_Identity=27.6497695852535, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788645, Length=218, Percent_Identity=28.8990825688073, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787086, Length=216, Percent_Identity=29.6296296296296, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1787087, Length=221, Percent_Identity=31.6742081447964, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788646, Length=170, Percent_Identity=32.9411764705882, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786873, Length=216, Percent_Identity=29.6296296296296, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI87082239, Length=204, Percent_Identity=27.4509803921569, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786874, Length=176, Percent_Identity=31.25, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI48994930, Length=126, Percent_Identity=25.3968253968254, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 24577; Mature: 24577

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSLSVVYHALPSMLAGCIVTVGNVTISLAMGLVLGVPMAVAQVYGGPWLRRLVAVYVWF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FRGVPILVLLFLCYGLFISLGLPADPFLICCLVLGCTSTAYQSQIFRGAIESLPQGQLNA
HCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHH
ARALGMGEGTGICSIILPQAMRLSIPGWANEFSILLKDSAICYVLGTQDIMARTSFVAAR
HHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHEEEECCEEEEEECCHHHHHHHHHHHHH
THEHLALYAAAGCIYFLLTLAVLKLLRRLEKRVHVPGYSTGMGMEGTGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDSLSVVYHALPSMLAGCIVTVGNVTISLAMGLVLGVPMAVAQVYGGPWLRRLVAVYVWF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FRGVPILVLLFLCYGLFISLGLPADPFLICCLVLGCTSTAYQSQIFRGAIESLPQGQLNA
HCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHH
ARALGMGEGTGICSIILPQAMRLSIPGWANEFSILLKDSAICYVLGTQDIMARTSFVAAR
HHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHEEEECCEEEEEECCHHHHHHHHHHHHH
THEHLALYAAAGCIYFLLTLAVLKLLRRLEKRVHVPGYSTGMGMEGTGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]