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The map label for this gene is fliY [H]

Identifier: 220905021

GI number: 220905021

Start: 2125265

End: 2126008

Strand: Direct

Name: fliY [H]

Synonym: Ddes_1758

Alternate gene names: 220905021

Gene position: 2125265-2126008 (Clockwise)

Preceding gene: 220905018

Following gene: 220905022

Centisome position: 73.96

GC content: 56.59

Gene sequence:

>744_bases
ATGAAAAAACTTCTTGTCTGCGCCCTGCTGGCTGCCGTCATGGCCGCTGTGCCCGCTTTTGCCAAAAAAAGCTATGTGAA
CGGCATTGACCCCAACTATCCGCCTTTCGCCTATATGGACGAAAAAACCGGCCAGCCCGCCGGCTTTGACGTGGATTCTC
TCAACTGGATAGCCAAGACCATGGGCTTCGAAATCACGCACAAACCCATGGCCTGGGACGGTATCATTCCCGCCCTCGTG
GCCAAGCAGATCGACATGGTGGACTCCGGCATGAGCATCACGCCCGAGCGCGCCAGAGTCGTGGACTTTTCTGAACCGTA
CTGGACGGTTTCGCGCGTGTTTATCGTGCCCGCAGACTCCACGCTGACCCCGCAGGACGTACTGACCAAAAAAGTCAAGC
TCGGCGTGCAGCGCGGCACATCCGAAGCCAACGCCATCAAGCAGGAACAGCAGGAAAAAGGCTATCCCTTTGAACTGCGC
TTCTACGAGTCCGCCCCCCTGGCTGTGGAAGACCTGCTCAACGGCCGCATTGAAGTGGCCCTGATGGACGAACTGCCCGC
CGACGACGCCATTGCCAAGGGCCGGGCTGTCAAAAAAGCCGGAACCCACGGCGAACCCGACAAGTTCGGTGTAGCCATGC
GCAAGGGCGATAAGGACCTGCACAAACTGATCAATGAGGGCTACAAAAAGCTCATGGCCGATCCGTACTGGCAGGAACTG
CAGAAAAAATATCTGAATAAATAA

Upstream 100 bases:

>100_bases
CTGCCCGGGTGCGCGCATCCACTTTTTCAGCGGCTGCCGCATGCTATTCCGCGGTTTTGCCGTACAAAGGCCACTGCTAT
TTCCGAGGGGGAAAACGAGC

Downstream 100 bases:

>100_bases
GCAACGCGAAAGCGCTGAACGGCAGGTGCGGACCGCCGGTTATTCCGGCGGTCCGCGCTGTTATGCCCAACCTACGGCAA
AACCCCGCATATGGACTCAC

Product: family 3 extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MKKLLVCALLAAVMAAVPAFAKKSYVNGIDPNYPPFAYMDEKTGQPAGFDVDSLNWIAKTMGFEITHKPMAWDGIIPALV
AKQIDMVDSGMSITPERARVVDFSEPYWTVSRVFIVPADSTLTPQDVLTKKVKLGVQRGTSEANAIKQEQQEKGYPFELR
FYESAPLAVEDLLNGRIEVALMDELPADDAIAKGRAVKKAGTHGEPDKFGVAMRKGDKDLHKLINEGYKKLMADPYWQEL
QKKYLNK

Sequences:

>Translated_247_residues
MKKLLVCALLAAVMAAVPAFAKKSYVNGIDPNYPPFAYMDEKTGQPAGFDVDSLNWIAKTMGFEITHKPMAWDGIIPALV
AKQIDMVDSGMSITPERARVVDFSEPYWTVSRVFIVPADSTLTPQDVLTKKVKLGVQRGTSEANAIKQEQQEKGYPFELR
FYESAPLAVEDLLNGRIEVALMDELPADDAIAKGRAVKKAGTHGEPDKFGVAMRKGDKDLHKLINEGYKKLMADPYWQEL
QKKYLNK
>Mature_247_residues
MKKLLVCALLAAVMAAVPAFAKKSYVNGIDPNYPPFAYMDEKTGQPAGFDVDSLNWIAKTMGFEITHKPMAWDGIIPALV
AKQIDMVDSGMSITPERARVVDFSEPYWTVSRVFIVPADSTLTPQDVLTKKVKLGVQRGTSEANAIKQEQQEKGYPFELR
FYESAPLAVEDLLNGRIEVALMDELPADDAIAKGRAVKKAGTHGEPDKFGVAMRKGDKDLHKLINEGYKKLMADPYWQEL
QKKYLNK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=256, Percent_Identity=31.25, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787085, Length=253, Percent_Identity=30.8300395256917, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1788649, Length=255, Percent_Identity=29.0196078431373, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1787088, Length=255, Percent_Identity=31.7647058823529, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788648, Length=227, Percent_Identity=28.6343612334802, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787031, Length=250, Percent_Identity=29.6, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27482; Mature: 27482

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLVCALLAAVMAAVPAFAKKSYVNGIDPNYPPFAYMDEKTGQPAGFDVDSLNWIAKT
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHH
MGFEITHKPMAWDGIIPALVAKQIDMVDSGMSITPERARVVDFSEPYWTVSRVFIVPADS
HCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEEEEEECCC
TLTPQDVLTKKVKLGVQRGTSEANAIKQEQQEKGYPFELRFYESAPLAVEDLLNGRIEVA
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCEEEE
LMDELPADDAIAKGRAVKKAGTHGEPDKFGVAMRKGDKDLHKLINEGYKKLMADPYWQEL
EECCCCCCHHHHCCCHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHH
QKKYLNK
HHHHCCC
>Mature Secondary Structure
MKKLLVCALLAAVMAAVPAFAKKSYVNGIDPNYPPFAYMDEKTGQPAGFDVDSLNWIAKT
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHH
MGFEITHKPMAWDGIIPALVAKQIDMVDSGMSITPERARVVDFSEPYWTVSRVFIVPADS
HCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEEEEEECCC
TLTPQDVLTKKVKLGVQRGTSEANAIKQEQQEKGYPFELRFYESAPLAVEDLLNGRIEVA
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCEEEE
LMDELPADDAIAKGRAVKKAGTHGEPDKFGVAMRKGDKDLHKLINEGYKKLMADPYWQEL
EECCCCCCHHHHCCCHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHH
QKKYLNK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]