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The map label for this gene is priA [H]

Identifier: 220904975

GI number: 220904975

Start: 2068214

End: 2070598

Strand: Reverse

Name: priA [H]

Synonym: Ddes_1711

Alternate gene names: 220904975

Gene position: 2070598-2068214 (Counterclockwise)

Preceding gene: 220904976

Following gene: 220904974

Centisome position: 72.06

GC content: 62.85

Gene sequence:

>2385_bases
ATGTATGCCAGCATTGCTCTTCTGAGTCCCCCTTATGCCACCTTGAGTTACGCCTTGCCGCCAGAATTTCCACAGGAGTT
CTGGCAGCCCGGCCTGCGCGTGGCCGTGCCGCTGGGCAGGGGAGACAAGGCGGCCCTGCGCGCCGGGGTTCTGCTTGAAA
CCAGCACCCACATTGACCTGCCCGCGGGGGTGGCCTGCAAAAGTGTTTGCTGGCCGCTGGAAGAAAAGGCTGTCCTCACG
CCCGAACTGTTGGCGCTGGCACAGGATCTTGCCCTGCGGCAAGCCCTCAGCCCCGGTCATATTCTGGGGCATGTGCTGCC
GCAAGGGCTGCGCCTTACCCGTGTGCGCCTGCGCCTGCTCCACGAAAAGGGAGCAGCCTCATGGCCCCTGGCCCGCATTC
GGGGGGCTGATGCGGCCACAAGGCAGCACCTTGCCCAGGCTCTTGCCCACGGGCAGGCCCGGCTGCTGCCGCCGGGAGCC
GATGCCGCCAGTGAGGAATTTTGCGTGTTGCGTGTGGACCCCCCCTGGCCGGTGCGGCCCTCTGCCACACGGCAGATAGA
AATTCTGGAGTTTCTGCACGAGCACGGGGCTGTAAGCCGCCGTCATCTTATCCGTTCGCTGGGGCAGGGGGCAGGGGTTC
CATTGCAGTCTCTGCTGGCCGCCGGACACGTCACCCTGACGCGGCAGGATGCCGAGGATGTGGTTGAGGCCGTGGAGCAG
AGCCTTTTGCCGCCGCCTCCAGCTTCTTTTACGCTCAATGATGATCAGGAGCGCGCTCTCGCGGATATGAAGGCGGCGCT
TGAAAACGCGCATTCCGGCGGGCGGGCTGCTTCGCGCCTGCTGTACGGGGTGACGGGCAGCGGCAAAACCGCTGTGTATC
TGGAGCTGGCCCGCGCCTGTCTTGAATCGGGCAGGAGCATGCTTTTGCTTGCGCCCGAGGTCGCGCTGGCCCACAAGCTG
CGGCGTGACGCCGCCTGCGCCCTGCCCCAGGTTCCACTTTTTTTCCATCACGGTTATCAGTCTCCCGCGCGCCGGGAAGA
TACCTACAGGCAACTGGCCGGGCGTGACGAACCCTGTCTGGTGGTAGGCACACGTTCGGCCCTGTTCTTGCCCGTACCGC
ACCTGGGCTGCATTGTGCTGGACGAAGAGCACGACGCTTCTTTCAAGCAGGATGAAAGCCTCGCCTATCAGGCCAAGGAG
GTGGCATGGTTTCGTATGGCGCAGATTCACGGCCTGCTGGTTCTCGGCTCCGCCACGCCTGACCTCAAGACCTTTCATGC
GTCGGAAAGCGGGCATCTGCCCATATTGCGCCTGCCCAGGCGTGTAGGCGACCGCGACCTGCCGCCTGTGGAGCTTGTGG
ACATAAGTTCCCTTTCGCCGGCGGCCACCTCCGAAACCGGTCTGCTGGCTCCCCGCAGCGAAGAAGCCCTGCGTGAGGTT
ATCAGCAGGGGCGAGCAGGCCGTGGTGCTGCTCAACCGTCGCGGCTATGCCCCGCTTATGTACTGCCTGGATTGCGGGCG
TACCCTGCGCTGCCCTCATTGTGAAATAGGCCTGACCTATCACAAGGGGCGCGAAAAGCTTGTTTGCCATTATTGCGGGT
ATACTCGTCCGTTTCCTTCGCCTTGCCCCGAATGCAAGGGAATGAATTTTTTGCCGATGGGTGAAGGCACAGAGCGCCTG
GGCGAACGCCTGGAGGTTCTGGCCGGAGCACCCGTGCTGCGGCTGGACAGGGACAGTACCCGCCGCCCCGGCCGTATGGA
GGAAATCCTGGCAGCCTTTGCCCGGCAGGAATCGTCCATTCTTGTGGGTACGCAGATGCTTTCCAAGGGGCATCATTTTC
CCAACGTGACCCTGGCTGTGGTGGCTGATGGCGACCTTGGGCTTAACCTGCCGGACTATCGCGCAGCGGAGCGCACCTTT
CAGCTTTTGGTGCAGTCGTCAGGCAGGGCCGGACGTGGCGACAAGGCCGGGCAGGTGCTCATACAGACGCGGGACGTGAG
CCATTATTGCTGGCAGTACGTGCGTGAGGCCGATTACGAGGGATTTTACGCGGCAGAACTGGCCCGCCGCCGGCTGCGCC
GGTATCCGCCGTTTGTGCGCCTGGCTCTCATACGCATTTCCCACGGCATCGAAGAAAGCGGAGGGCCTGCGGCCCTCAAT
GACCTGGCAGTGGCCCTGCGCGGCCGCTCGACGGAACTGGGCCTGCAACTGCTGGGTCCCGCTCCTGCGCCGCTGGCGTT
GCTGCGGGGCAGGCGGCGGTATACCTGCCTTGTGAAAGGGCAGGACTGGCAGGCCATCCGGTCGCTGTATTTTTTTGCTC
TTTCACAAAAATGCGCTAAAAATCTACGACTTTTTCTTGACCTTGATCCTGTAAATATGTTGTGA

Upstream 100 bases:

>100_bases
GTGCTCAAAAATCTGCGCAGGCGTTGCAGGCAGGCTTCCGGCGGGCCGGGCCTTGTGCAGAGGCTTGCTTTCCCCGCCGT
ATTGGTTAACTCTGCCCCAC

Downstream 100 bases:

>100_bases
ATGGCAGTTGCATTTTTCTGTTGGCTGTGGGGTGCCGCCGCTTCTTCTCCGAAGAAGCCAGGGCGGCCAATTTTTTAATG
CAGACTGGCCCACTGGGGGC

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 794; Mature: 794

Protein sequence:

>794_residues
MYASIALLSPPYATLSYALPPEFPQEFWQPGLRVAVPLGRGDKAALRAGVLLETSTHIDLPAGVACKSVCWPLEEKAVLT
PELLALAQDLALRQALSPGHILGHVLPQGLRLTRVRLRLLHEKGAASWPLARIRGADAATRQHLAQALAHGQARLLPPGA
DAASEEFCVLRVDPPWPVRPSATRQIEILEFLHEHGAVSRRHLIRSLGQGAGVPLQSLLAAGHVTLTRQDAEDVVEAVEQ
SLLPPPPASFTLNDDQERALADMKAALENAHSGGRAASRLLYGVTGSGKTAVYLELARACLESGRSMLLLAPEVALAHKL
RRDAACALPQVPLFFHHGYQSPARREDTYRQLAGRDEPCLVVGTRSALFLPVPHLGCIVLDEEHDASFKQDESLAYQAKE
VAWFRMAQIHGLLVLGSATPDLKTFHASESGHLPILRLPRRVGDRDLPPVELVDISSLSPAATSETGLLAPRSEEALREV
ISRGEQAVVLLNRRGYAPLMYCLDCGRTLRCPHCEIGLTYHKGREKLVCHYCGYTRPFPSPCPECKGMNFLPMGEGTERL
GERLEVLAGAPVLRLDRDSTRRPGRMEEILAAFARQESSILVGTQMLSKGHHFPNVTLAVVADGDLGLNLPDYRAAERTF
QLLVQSSGRAGRGDKAGQVLIQTRDVSHYCWQYVREADYEGFYAAELARRRLRRYPPFVRLALIRISHGIEESGGPAALN
DLAVALRGRSTELGLQLLGPAPAPLALLRGRRRYTCLVKGQDWQAIRSLYFFALSQKCAKNLRLFLDLDPVNML

Sequences:

>Translated_794_residues
MYASIALLSPPYATLSYALPPEFPQEFWQPGLRVAVPLGRGDKAALRAGVLLETSTHIDLPAGVACKSVCWPLEEKAVLT
PELLALAQDLALRQALSPGHILGHVLPQGLRLTRVRLRLLHEKGAASWPLARIRGADAATRQHLAQALAHGQARLLPPGA
DAASEEFCVLRVDPPWPVRPSATRQIEILEFLHEHGAVSRRHLIRSLGQGAGVPLQSLLAAGHVTLTRQDAEDVVEAVEQ
SLLPPPPASFTLNDDQERALADMKAALENAHSGGRAASRLLYGVTGSGKTAVYLELARACLESGRSMLLLAPEVALAHKL
RRDAACALPQVPLFFHHGYQSPARREDTYRQLAGRDEPCLVVGTRSALFLPVPHLGCIVLDEEHDASFKQDESLAYQAKE
VAWFRMAQIHGLLVLGSATPDLKTFHASESGHLPILRLPRRVGDRDLPPVELVDISSLSPAATSETGLLAPRSEEALREV
ISRGEQAVVLLNRRGYAPLMYCLDCGRTLRCPHCEIGLTYHKGREKLVCHYCGYTRPFPSPCPECKGMNFLPMGEGTERL
GERLEVLAGAPVLRLDRDSTRRPGRMEEILAAFARQESSILVGTQMLSKGHHFPNVTLAVVADGDLGLNLPDYRAAERTF
QLLVQSSGRAGRGDKAGQVLIQTRDVSHYCWQYVREADYEGFYAAELARRRLRRYPPFVRLALIRISHGIEESGGPAALN
DLAVALRGRSTELGLQLLGPAPAPLALLRGRRRYTCLVKGQDWQAIRSLYFFALSQKCAKNLRLFLDLDPVNML
>Mature_794_residues
MYASIALLSPPYATLSYALPPEFPQEFWQPGLRVAVPLGRGDKAALRAGVLLETSTHIDLPAGVACKSVCWPLEEKAVLT
PELLALAQDLALRQALSPGHILGHVLPQGLRLTRVRLRLLHEKGAASWPLARIRGADAATRQHLAQALAHGQARLLPPGA
DAASEEFCVLRVDPPWPVRPSATRQIEILEFLHEHGAVSRRHLIRSLGQGAGVPLQSLLAAGHVTLTRQDAEDVVEAVEQ
SLLPPPPASFTLNDDQERALADMKAALENAHSGGRAASRLLYGVTGSGKTAVYLELARACLESGRSMLLLAPEVALAHKL
RRDAACALPQVPLFFHHGYQSPARREDTYRQLAGRDEPCLVVGTRSALFLPVPHLGCIVLDEEHDASFKQDESLAYQAKE
VAWFRMAQIHGLLVLGSATPDLKTFHASESGHLPILRLPRRVGDRDLPPVELVDISSLSPAATSETGLLAPRSEEALREV
ISRGEQAVVLLNRRGYAPLMYCLDCGRTLRCPHCEIGLTYHKGREKLVCHYCGYTRPFPSPCPECKGMNFLPMGEGTERL
GERLEVLAGAPVLRLDRDSTRRPGRMEEILAAFARQESSILVGTQMLSKGHHFPNVTLAVVADGDLGLNLPDYRAAERTF
QLLVQSSGRAGRGDKAGQVLIQTRDVSHYCWQYVREADYEGFYAAELARRRLRRYPPFVRLALIRISHGIEESGGPAALN
DLAVALRGRSTELGLQLLGPAPAPLALLRGRRRYTCLVKGQDWQAIRSLYFFALSQKCAKNLRLFLDLDPVNML

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=555, Percent_Identity=34.5945945945946, Blast_Score=301, Evalue=9e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 87100; Mature: 87100

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYASIALLSPPYATLSYALPPEFPQEFWQPGLRVAVPLGRGDKAALRAGVLLETSTHIDL
CCEEEEECCCCHHHEEECCCCCCHHHHCCCCCEEEEECCCCCHHHHHCCEEEECCCCCCC
PAGVACKSVCWPLEEKAVLTPELLALAQDLALRQALSPGHILGHVLPQGLRLTRVRLRLL
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHH
HEKGAASWPLARIRGADAATRQHLAQALAHGQARLLPPGADAASEEFCVLRVDPPWPVRP
HHCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEECCCCCCCC
SATRQIEILEFLHEHGAVSRRHLIRSLGQGAGVPLQSLLAAGHVTLTRQDAEDVVEAVEQ
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHH
SLLPPPPASFTLNDDQERALADMKAALENAHSGGRAASRLLYGVTGSGKTAVYLELARAC
HCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCHHHHHEEEEECCCCCEEHHHHHHHHH
LESGRSMLLLAPEVALAHKLRRDAACALPQVPLFFHHGYQSPARREDTYRQLAGRDEPCL
HHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHEECCCCCCCHHHHHHHHHHCCCCCEE
VVGTRSALFLPVPHLGCIVLDEEHDASFKQDESLAYQAKEVAWFRMAQIHGLLVLGSATP
EEECCCEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCC
DLKTFHASESGHLPILRLPRRVGDRDLPPVELVDISSLSPAATSETGLLAPRSEEALREV
CCCEEECCCCCCCCCEECCHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
ISRGEQAVVLLNRRGYAPLMYCLDCGRTLRCPHCEIGLTYHKGREKLVCHYCGYTRPFPS
HHCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCEECCEEECCCCEEEEEECCCCCCCCC
PCPECKGMNFLPMGEGTERLGERLEVLAGAPVLRLDRDSTRRPGRMEEILAAFARQESSI
CCCCCCCCCEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCE
LVGTQMLSKGHHFPNVTLAVVADGDLGLNLPDYRAAERTFQLLVQSSGRAGRGDKAGQVL
EEEHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEE
IQTRDVSHYCWQYVREADYEGFYAAELARRRLRRYPPFVRLALIRISHGIEESGGPAALN
EEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHH
DLAVALRGRSTELGLQLLGPAPAPLALLRGRRRYTCLVKGQDWQAIRSLYFFALSQKCAK
HHHHHHCCCCHHCCEEEECCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHC
NLRLFLDLDPVNML
CCEEEEECCCCCCC
>Mature Secondary Structure
MYASIALLSPPYATLSYALPPEFPQEFWQPGLRVAVPLGRGDKAALRAGVLLETSTHIDL
CCEEEEECCCCHHHEEECCCCCCHHHHCCCCCEEEEECCCCCHHHHHCCEEEECCCCCCC
PAGVACKSVCWPLEEKAVLTPELLALAQDLALRQALSPGHILGHVLPQGLRLTRVRLRLL
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHH
HEKGAASWPLARIRGADAATRQHLAQALAHGQARLLPPGADAASEEFCVLRVDPPWPVRP
HHCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEECCCCCCCC
SATRQIEILEFLHEHGAVSRRHLIRSLGQGAGVPLQSLLAAGHVTLTRQDAEDVVEAVEQ
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHH
SLLPPPPASFTLNDDQERALADMKAALENAHSGGRAASRLLYGVTGSGKTAVYLELARAC
HCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCHHHHHEEEEECCCCCEEHHHHHHHHH
LESGRSMLLLAPEVALAHKLRRDAACALPQVPLFFHHGYQSPARREDTYRQLAGRDEPCL
HHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHEECCCCCCCHHHHHHHHHHCCCCCEE
VVGTRSALFLPVPHLGCIVLDEEHDASFKQDESLAYQAKEVAWFRMAQIHGLLVLGSATP
EEECCCEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCC
DLKTFHASESGHLPILRLPRRVGDRDLPPVELVDISSLSPAATSETGLLAPRSEEALREV
CCCEEECCCCCCCCCEECCHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
ISRGEQAVVLLNRRGYAPLMYCLDCGRTLRCPHCEIGLTYHKGREKLVCHYCGYTRPFPS
HHCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCEECCEEECCCCEEEEEECCCCCCCCC
PCPECKGMNFLPMGEGTERLGERLEVLAGAPVLRLDRDSTRRPGRMEEILAAFARQESSI
CCCCCCCCCEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCE
LVGTQMLSKGHHFPNVTLAVVADGDLGLNLPDYRAAERTFQLLVQSSGRAGRGDKAGQVL
EEEHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEE
IQTRDVSHYCWQYVREADYEGFYAAELARRRLRRYPPFVRLALIRISHGIEESGGPAALN
EEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHH
DLAVALRGRSTELGLQLLGPAPAPLALLRGRRRYTCLVKGQDWQAIRSLYFFALSQKCAK
HHHHHHCCCCHHCCEEEECCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHC
NLRLFLDLDPVNML
CCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]