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The map label for this gene is mdlC [H]

Identifier: 220904969

GI number: 220904969

Start: 2057212

End: 2058834

Strand: Direct

Name: mdlC [H]

Synonym: Ddes_1705

Alternate gene names: 220904969

Gene position: 2057212-2058834 (Clockwise)

Preceding gene: 220904967

Following gene: 220904970

Centisome position: 71.59

GC content: 59.89

Gene sequence:

>1623_bases
ATGGCAAAAACCATTCGTCAGGTCACATTTGAACTTTTACGCCAGCTGGGCATTACCACAATCTTTGGCAACCCCGGCTC
AACTGAAGAAACATTTTTGAAAGATTTTCCTAAAGATTTCATATATATTCAGACTCTTCAAGAAGCTTCAGCCGTGGGCG
CCGCCGACGGCTATGCCCAGGCAACACGTAAGCCCGCACTGGTAAACGTGCACACTTCTGCCGGTCTTTGCAATGCCATG
AGCAATATTCTTACTGCCAGCATGAACAAGACGCCTCTCATTATTACAGCTGGCAACCAGACGCGCGAAATGCTCCTTAT
GGAGCCGTGGCTCACCAACGTGGAGCCTGCCGAACTGCCCAAGCCCTGGGTCAAGTGGAGCTACGAACCTGTACGCGCCG
AAGATGTGCCTGCCGCCTTCATGCGAGCCTATGCCATGGCCATGCAGCCCCCGACCGGGCCTGTTTTCCTCTCCATTCCG
CTTGATGACTGGGACAAGCCTGCACTGGGCGAACCCGTGGCACGCAGCCTTGCCGGCCGCGTTGGGCCGGACCCCAAACG
CCTGGCCGAATTTGCCGACCTGCTCTCCAAGGCCAAAAATCCCGCTCTCATCTACGGCGCGGCCATTGCCAGGGGAGACG
GCTGGAAACAGGCCGTGGCCCTGGCTGAAAAACTCAATGCCAAGGTATGGGCCGCCCCCGCCTCGGAGCGCCCGCCCTTC
CCCGAAACCCATCCGCTGTACGCTGGCGGCCTGCCCTTTGCCATCGGGCCGCTAGCCAAAAAGCTTGAAGGGCACGATGT
GGCCATTGTGATCGGCGCGCCCGTATTCCGTTATTATCCGTATGTTGAGGGCCAGTATATTCCTGACGGTCTGCGCCTGC
TGCATATTTCCGATGACCCCGCAGAGACGGGCCGCGCGCCGGTGGGCGACAGCATGCTGTGCGATGCGGTGCTGGCCCTG
GAAGCGCTGGAAACGCTGGTGCAGGCGCGCTCTGCCACATGCGAAGTCTGCAGGCAGCCCCATTGTATGGCTCCCCATGC
AGCCGCTCCTGCTGCGGCCCCCGGCAAGGTGATGACCGCCCTGGAACTTTTCAAGACGCTGCGTGAATGCACACCCGCTG
AAGCTGTGCTGGTGGAAGAATCTCCCTCCAACCTGGGCGAACTGCATACGGCATGGCCGGTGGAACGTCCTGACTCTTTC
TATACTTTTGCCAGCGGCAGCCTGGGCTGGAACCTGCCCGCCAGCGTGGGCATCGCCCTTGCCGAGCGCGACAGCGGGCG
CAACCGCCCGGTAGTCGCCATTATCGGCGACGGCTCCATGCAGTATTCTGTTCAGGGACTGTGGACAGCGGCACAGCAGG
GACTGCCCATCCTTTTTGTCATACCGAGAAACGGCGAATACGCCATTCTGAAGTCTTTTGCCGTTCTGGAAGAAACCCCC
GGCGTCCCGGGCCTTGACCTGCCGGGCCTTGATACGGTTGCCATCGCCAAGGGCTACGGTTGCGAAGCCATACGCGTTGA
AACCGTCGACGAAGTGCGCAAGGTTTGCGCCGAAGCTTTCAAACGCTCCGGCCCCACCCTTATAGAAGTGCCCATCGTCC
CCACCATCCCGCCCCTGCTTTAG

Upstream 100 bases:

>100_bases
TTTCTTATAACAGATTGCCGAAGATAGGATTGTCTACTAATAAAACACATGCTATCTCTAACGCAACACTAACCAGATGC
GGAATCCCGTGGAGGAAATC

Downstream 100 bases:

>100_bases
GCCAGAACCAAACACCATACCCGGATCCCGGCGGGTCCGGGTATGGCTAAAAGGAACCGTCCATGCTCGAAACAATAATC
ATGGCCCTGCTGCCCATTGT

Product: thiamine pyrophosphate TPP binding domain-containing protein

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MAKTIRQVTFELLRQLGITTIFGNPGSTEETFLKDFPKDFIYIQTLQEASAVGAADGYAQATRKPALVNVHTSAGLCNAM
SNILTASMNKTPLIITAGNQTREMLLMEPWLTNVEPAELPKPWVKWSYEPVRAEDVPAAFMRAYAMAMQPPTGPVFLSIP
LDDWDKPALGEPVARSLAGRVGPDPKRLAEFADLLSKAKNPALIYGAAIARGDGWKQAVALAEKLNAKVWAAPASERPPF
PETHPLYAGGLPFAIGPLAKKLEGHDVAIVIGAPVFRYYPYVEGQYIPDGLRLLHISDDPAETGRAPVGDSMLCDAVLAL
EALETLVQARSATCEVCRQPHCMAPHAAAPAAAPGKVMTALELFKTLRECTPAEAVLVEESPSNLGELHTAWPVERPDSF
YTFASGSLGWNLPASVGIALAERDSGRNRPVVAIIGDGSMQYSVQGLWTAAQQGLPILFVIPRNGEYAILKSFAVLEETP
GVPGLDLPGLDTVAIAKGYGCEAIRVETVDEVRKVCAEAFKRSGPTLIEVPIVPTIPPLL

Sequences:

>Translated_540_residues
MAKTIRQVTFELLRQLGITTIFGNPGSTEETFLKDFPKDFIYIQTLQEASAVGAADGYAQATRKPALVNVHTSAGLCNAM
SNILTASMNKTPLIITAGNQTREMLLMEPWLTNVEPAELPKPWVKWSYEPVRAEDVPAAFMRAYAMAMQPPTGPVFLSIP
LDDWDKPALGEPVARSLAGRVGPDPKRLAEFADLLSKAKNPALIYGAAIARGDGWKQAVALAEKLNAKVWAAPASERPPF
PETHPLYAGGLPFAIGPLAKKLEGHDVAIVIGAPVFRYYPYVEGQYIPDGLRLLHISDDPAETGRAPVGDSMLCDAVLAL
EALETLVQARSATCEVCRQPHCMAPHAAAPAAAPGKVMTALELFKTLRECTPAEAVLVEESPSNLGELHTAWPVERPDSF
YTFASGSLGWNLPASVGIALAERDSGRNRPVVAIIGDGSMQYSVQGLWTAAQQGLPILFVIPRNGEYAILKSFAVLEETP
GVPGLDLPGLDTVAIAKGYGCEAIRVETVDEVRKVCAEAFKRSGPTLIEVPIVPTIPPLL
>Mature_539_residues
AKTIRQVTFELLRQLGITTIFGNPGSTEETFLKDFPKDFIYIQTLQEASAVGAADGYAQATRKPALVNVHTSAGLCNAMS
NILTASMNKTPLIITAGNQTREMLLMEPWLTNVEPAELPKPWVKWSYEPVRAEDVPAAFMRAYAMAMQPPTGPVFLSIPL
DDWDKPALGEPVARSLAGRVGPDPKRLAEFADLLSKAKNPALIYGAAIARGDGWKQAVALAEKLNAKVWAAPASERPPFP
ETHPLYAGGLPFAIGPLAKKLEGHDVAIVIGAPVFRYYPYVEGQYIPDGLRLLHISDDPAETGRAPVGDSMLCDAVLALE
ALETLVQARSATCEVCRQPHCMAPHAAAPAAAPGKVMTALELFKTLRECTPAEAVLVEESPSNLGELHTAWPVERPDSFY
TFASGSLGWNLPASVGIALAERDSGRNRPVVAIIGDGSMQYSVQGLWTAAQQGLPILFVIPRNGEYAILKSFAVLEETPG
VPGLDLPGLDTVAIAKGYGCEAIRVETVDEVRKVCAEAFKRSGPTLIEVPIVPTIPPLL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=567, Percent_Identity=24.6913580246914, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87081685, Length=534, Percent_Identity=24.3445692883895, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1786717, Length=538, Percent_Identity=24.1635687732342, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=22.0216606498195, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 57971; Mature: 57840

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTIRQVTFELLRQLGITTIFGNPGSTEETFLKDFPKDFIYIQTLQEASAVGAADGYAQ
CCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEHHHHHHCCCCCCHHH
ATRKPALVNVHTSAGLCNAMSNILTASMNKTPLIITAGNQTREMLLMEPWLTNVEPAELP
HCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCCCCHHHCC
KPWVKWSYEPVRAEDVPAAFMRAYAMAMQPPTGPVFLSIPLDDWDKPALGEPVARSLAGR
CHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCC
VGPDPKRLAEFADLLSKAKNPALIYGAAIARGDGWKQAVALAEKLNAKVWAAPASERPPF
CCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHCCEEEECCCCCCCCC
PETHPLYAGGLPFAIGPLAKKLEGHDVAIVIGAPVFRYYPYVEGQYIPDGLRLLHISDDP
CCCCCCEECCCCHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCEECCCCEEEEEECCCC
AETGRAPVGDSMLCDAVLALEALETLVQARSATCEVCRQPHCMAPHAAAPAAAPGKVMTA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
LELFKTLRECTPAEAVLVEESPSNLGELHTAWPVERPDSFYTFASGSLGWNLPASVGIAL
HHHHHHHHHCCCCCEEEEECCCCCHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHCEEE
AERDSGRNRPVVAIIGDGSMQYSVQGLWTAAQQGLPILFVIPRNGEYAILKSFAVLEETP
EECCCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHCC
GVPGLDLPGLDTVAIAKGYGCEAIRVETVDEVRKVCAEAFKRSGPTLIEVPIVPTIPPLL
CCCCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure 
AKTIRQVTFELLRQLGITTIFGNPGSTEETFLKDFPKDFIYIQTLQEASAVGAADGYAQ
CHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEHHHHHHCCCCCCHHH
ATRKPALVNVHTSAGLCNAMSNILTASMNKTPLIITAGNQTREMLLMEPWLTNVEPAELP
HCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCCCCHHHCC
KPWVKWSYEPVRAEDVPAAFMRAYAMAMQPPTGPVFLSIPLDDWDKPALGEPVARSLAGR
CHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCC
VGPDPKRLAEFADLLSKAKNPALIYGAAIARGDGWKQAVALAEKLNAKVWAAPASERPPF
CCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHCCEEEECCCCCCCCC
PETHPLYAGGLPFAIGPLAKKLEGHDVAIVIGAPVFRYYPYVEGQYIPDGLRLLHISDDP
CCCCCCEECCCCHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCEECCCCEEEEEECCCC
AETGRAPVGDSMLCDAVLALEALETLVQARSATCEVCRQPHCMAPHAAAPAAAPGKVMTA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
LELFKTLRECTPAEAVLVEESPSNLGELHTAWPVERPDSFYTFASGSLGWNLPASVGIAL
HHHHHHHHHCCCCCEEEEECCCCCHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHCEEE
AERDSGRNRPVVAIIGDGSMQYSVQGLWTAAQQGLPILFVIPRNGEYAILKSFAVLEETP
EECCCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHCC
GVPGLDLPGLDTVAIAKGYGCEAIRVETVDEVRKVCAEAFKRSGPTLIEVPIVPTIPPLL
CCCCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]