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The map label for this gene is mrpA [H]

Identifier: 220904938

GI number: 220904938

Start: 2009193

End: 2011148

Strand: Reverse

Name: mrpA [H]

Synonym: Ddes_1673

Alternate gene names: 220904938

Gene position: 2011148-2009193 (Counterclockwise)

Preceding gene: 220904956

Following gene: 220904937

Centisome position: 69.99

GC content: 55.98

Gene sequence:

>1956_bases
ATGAATACACTTGTTTTTTGCTGTGTGGCGCTGCCATTTGCTGTGGCGCTTGTTCTGTATTTCACACAGCTTGACCGCAC
CCGCAAGCTTCTCGTGCCTGCAGCGGTTGCGGTGATGGCTCTGGCTGCCGTGATTATGGGTGCTCACGGAACGTTCCGAC
TTGAGGCCGAAACTTTCATGGGCCTTCCGCTGGACAGCCTGTTCAGCTTGCTGGATCTTTTGCTGCTGCTCTATATTTTG
GGCTTGGGCTGGAAGCTGGGCAGCCGCACGGTCATGGGCATGACTGTGCTGCAATTGATTGGGTTGCTTTACCTCAAGTT
TGTTCTGGCGGACGGTTCCGCCCCCATTACTGCCTTTGCGCCCGATGGCCTGTCGCTCATCATGGTTATCATAATCACCG
TGGTGGGTGGCCTGATCACCATTTACGGCCTGGGCTATATGGATATTCATGAAGAGCACCTGCACCTGCGGGTTTCTCGC
AAACCGCGGTTTTTCGCCATTATTTTCTGCTTTCTCGGGGCGATGAACGGGCTTGTGCTTTCAAACAATCTTTCGTGGAT
GTTCTTTTTCTGGGAAGTCACGACCCTGTGTTCCTACCTGCTGATCAGCCACGATCAGACACAGGAAGCCAATGCCAACG
CCTACCGTGCATTGTGGATGAACGTTCTTGGCGGTCTTGCCTTTGTTTCTGCCATGCTTTTCATTCAGAAGAGCCTTGGC
ACACTGTCCACTGAAGTTGTATTGCACAAGATGACGGCCATGGACGTGAAAACCACGGCCATGCTGCTGCCGTTTGCCTT
TTTTTGCCTGGCGGCCTTTACCAAGTCGGCGCAGGTTCCCTTTGAAAGCTGGCTGTGCGGTGCCATGGTTGCTCCCACCC
CGGTTTCGGCCCTGCTGCACTCGGCCACCATGGTCAAGGCGGGCACATACCTGCTGCTGCGCATGGCCCCGGCCTTCGCT
GACACCACCATGTCCACCATTGTGGCGCTCTTCGGCGCGTTCACCTTTGTGGGGACATGCATTCTGGCGGTCAGCCAAAG
TAATGCCAAAAAGATCCTGGCATACTCAACCATTGCCAACCTGGGGCTTATCATCGCCTGCGTTGGCATAAATACGGCGG
CATCCATGATGGCGGCCACCACCATCATCATCTACCACTCGGTCTCCAAGGGCCTGCTCTTCATGTGCGTGGGCGCCATT
GAACAGCGCATCGGCTCCAGAGATATTGAAGACATGCGCGGCCTGTACAGCAAGATGCCCCGCACGGCCATTATTACGGC
CATAGGCATCTTCACCATGATGCTGCCGCCTTTCGGCATGCTCATAGGCAAGTGGATGGCAATTGAGGCCATTGCCCGTG
CCACCCAGGCCATGACGCCCATCATTTTCTTCATTGCCCTCGGCTCGGCCTTTACTGTGCTGTTCTGGGCGCGGTGGGCA
GGCATACTCGTATCTTCGGCCAACCTGCATGACCGGCCCCCTCACGGCAACCCCAAGGCCACGGTCATGTTTGCCCTGCG
TTCGCTCTGCGGGCTTGCCGTGGTGTTCTCCTTCATTTCGCCTCTGGTACTGGAAACATTTGTCGAGCCTTCTGTGGCTG
GCGTATACGCCCGCCTTGGTCTCAAGACCGAAGGCTTCATACCTGGCGCATCGCTTACAGGTGGGGCGGGCTATTTCTGG
ATTTACCTGCTCTTCATCCTGCTTGGCCTGGGGGCCTGGATTGCCTGGAAGGCCGCCAGAAAGGTTTCAAACTCGGCGCA
TGCCCAGCCCTATTTCTCGGGGCTGACGCAGGAACAGGCGGGGCAGATCGGTTTCAAGGGCCCCATGAATGCCTTTGAAC
CCGTGCGTCTGTCCAACTTTTACCTGACCCAGTACTTTGGCGAAGGCACTATAACACGGGCCATTGATATTATATCCACG
GCCTTTCTCATCGTCCTGGTAGGAGGTCTGCTCTAA

Upstream 100 bases:

>100_bases
GCATTGGAAAGGCAAGGAGGAAACATATTAATTTTATGTTGTGAGGGGATTACCACAGGGTGAAGTTTTTCACAGCTCAC
AATATAGTGGGGCAAAAATT

Downstream 100 bases:

>100_bases
TGCTGTCCATCCTCAGTGCTGTCGGCGGATTGATATTGTCGCCCCTGGTGGGGGGCCTGCTTACCGGGGTTGACCGCCGT
CTCACGGCGCGGCTGCAATC

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MNTLVFCCVALPFAVALVLYFTQLDRTRKLLVPAAVAVMALAAVIMGAHGTFRLEAETFMGLPLDSLFSLLDLLLLLYIL
GLGWKLGSRTVMGMTVLQLIGLLYLKFVLADGSAPITAFAPDGLSLIMVIIITVVGGLITIYGLGYMDIHEEHLHLRVSR
KPRFFAIIFCFLGAMNGLVLSNNLSWMFFFWEVTTLCSYLLISHDQTQEANANAYRALWMNVLGGLAFVSAMLFIQKSLG
TLSTEVVLHKMTAMDVKTTAMLLPFAFFCLAAFTKSAQVPFESWLCGAMVAPTPVSALLHSATMVKAGTYLLLRMAPAFA
DTTMSTIVALFGAFTFVGTCILAVSQSNAKKILAYSTIANLGLIIACVGINTAASMMAATTIIIYHSVSKGLLFMCVGAI
EQRIGSRDIEDMRGLYSKMPRTAIITAIGIFTMMLPPFGMLIGKWMAIEAIARATQAMTPIIFFIALGSAFTVLFWARWA
GILVSSANLHDRPPHGNPKATVMFALRSLCGLAVVFSFISPLVLETFVEPSVAGVYARLGLKTEGFIPGASLTGGAGYFW
IYLLFILLGLGAWIAWKAARKVSNSAHAQPYFSGLTQEQAGQIGFKGPMNAFEPVRLSNFYLTQYFGEGTITRAIDIIST
AFLIVLVGGLL

Sequences:

>Translated_651_residues
MNTLVFCCVALPFAVALVLYFTQLDRTRKLLVPAAVAVMALAAVIMGAHGTFRLEAETFMGLPLDSLFSLLDLLLLLYIL
GLGWKLGSRTVMGMTVLQLIGLLYLKFVLADGSAPITAFAPDGLSLIMVIIITVVGGLITIYGLGYMDIHEEHLHLRVSR
KPRFFAIIFCFLGAMNGLVLSNNLSWMFFFWEVTTLCSYLLISHDQTQEANANAYRALWMNVLGGLAFVSAMLFIQKSLG
TLSTEVVLHKMTAMDVKTTAMLLPFAFFCLAAFTKSAQVPFESWLCGAMVAPTPVSALLHSATMVKAGTYLLLRMAPAFA
DTTMSTIVALFGAFTFVGTCILAVSQSNAKKILAYSTIANLGLIIACVGINTAASMMAATTIIIYHSVSKGLLFMCVGAI
EQRIGSRDIEDMRGLYSKMPRTAIITAIGIFTMMLPPFGMLIGKWMAIEAIARATQAMTPIIFFIALGSAFTVLFWARWA
GILVSSANLHDRPPHGNPKATVMFALRSLCGLAVVFSFISPLVLETFVEPSVAGVYARLGLKTEGFIPGASLTGGAGYFW
IYLLFILLGLGAWIAWKAARKVSNSAHAQPYFSGLTQEQAGQIGFKGPMNAFEPVRLSNFYLTQYFGEGTITRAIDIIST
AFLIVLVGGLL
>Mature_651_residues
MNTLVFCCVALPFAVALVLYFTQLDRTRKLLVPAAVAVMALAAVIMGAHGTFRLEAETFMGLPLDSLFSLLDLLLLLYIL
GLGWKLGSRTVMGMTVLQLIGLLYLKFVLADGSAPITAFAPDGLSLIMVIIITVVGGLITIYGLGYMDIHEEHLHLRVSR
KPRFFAIIFCFLGAMNGLVLSNNLSWMFFFWEVTTLCSYLLISHDQTQEANANAYRALWMNVLGGLAFVSAMLFIQKSLG
TLSTEVVLHKMTAMDVKTTAMLLPFAFFCLAAFTKSAQVPFESWLCGAMVAPTPVSALLHSATMVKAGTYLLLRMAPAFA
DTTMSTIVALFGAFTFVGTCILAVSQSNAKKILAYSTIANLGLIIACVGINTAASMMAATTIIIYHSVSKGLLFMCVGAI
EQRIGSRDIEDMRGLYSKMPRTAIITAIGIFTMMLPPFGMLIGKWMAIEAIARATQAMTPIIFFIALGSAFTVLFWARWA
GILVSSANLHDRPPHGNPKATVMFALRSLCGLAVVFSFISPLVLETFVEPSVAGVYARLGLKTEGFIPGASLTGGAGYFW
IYLLFILLGLGAWIAWKAARKVSNSAHAQPYFSGLTQEQAGQIGFKGPMNAFEPVRLSNFYLTQYFGEGTITRAIDIIST
AFLIVLVGGLL

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=325, Percent_Identity=33.8461538461538, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1788829, Length=433, Percent_Identity=29.3302540415704, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1788614, Length=303, Percent_Identity=35.3135313531353, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788831, Length=344, Percent_Identity=29.6511627906977, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1788827, Length=435, Percent_Identity=25.9770114942529, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788613, Length=345, Percent_Identity=25.7971014492754, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI2367154, Length=296, Percent_Identity=25, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 70492; Mature: 70492

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLVFCCVALPFAVALVLYFTQLDRTRKLLVPAAVAVMALAAVIMGAHGTFRLEAETFM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHC
GLPLDSLFSLLDLLLLLYILGLGWKLGSRTVMGMTVLQLIGLLYLKFVLADGSAPITAFA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
PDGLSLIMVIIITVVGGLITIYGLGYMDIHEEHLHLRVSRKPRFFAIIFCFLGAMNGLVL
CCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHEEEECCCCCHHHHHHHHHHHCCCEEE
SNNLSWMFFFWEVTTLCSYLLISHDQTQEANANAYRALWMNVLGGLAFVSAMLFIQKSLG
ECCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLSTEVVLHKMTAMDVKTTAMLLPFAFFCLAAFTKSAQVPFESWLCGAMVAPTPVSALLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHH
SATMVKAGTYLLLRMAPAFADTTMSTIVALFGAFTFVGTCILAVSQSNAKKILAYSTIAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LGLIIACVGINTAASMMAATTIIIYHSVSKGLLFMCVGAIEQRIGSRDIEDMRGLYSKMP
HHHHHHHHCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
RTAIITAIGIFTMMLPPFGMLIGKWMAIEAIARATQAMTPIIFFIALGSAFTVLFWARWA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GILVSSANLHDRPPHGNPKATVMFALRSLCGLAVVFSFISPLVLETFVEPSVAGVYARLG
HHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
LKTEGFIPGASLTGGAGYFWIYLLFILLGLGAWIAWKAARKVSNSAHAQPYFSGLTQEQA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHC
GQIGFKGPMNAFEPVRLSNFYLTQYFGEGTITRAIDIISTAFLIVLVGGLL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTLVFCCVALPFAVALVLYFTQLDRTRKLLVPAAVAVMALAAVIMGAHGTFRLEAETFM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHC
GLPLDSLFSLLDLLLLLYILGLGWKLGSRTVMGMTVLQLIGLLYLKFVLADGSAPITAFA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
PDGLSLIMVIIITVVGGLITIYGLGYMDIHEEHLHLRVSRKPRFFAIIFCFLGAMNGLVL
CCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHEEEECCCCCHHHHHHHHHHHCCCEEE
SNNLSWMFFFWEVTTLCSYLLISHDQTQEANANAYRALWMNVLGGLAFVSAMLFIQKSLG
ECCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLSTEVVLHKMTAMDVKTTAMLLPFAFFCLAAFTKSAQVPFESWLCGAMVAPTPVSALLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHH
SATMVKAGTYLLLRMAPAFADTTMSTIVALFGAFTFVGTCILAVSQSNAKKILAYSTIAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LGLIIACVGINTAASMMAATTIIIYHSVSKGLLFMCVGAIEQRIGSRDIEDMRGLYSKMP
HHHHHHHHCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
RTAIITAIGIFTMMLPPFGMLIGKWMAIEAIARATQAMTPIIFFIALGSAFTVLFWARWA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GILVSSANLHDRPPHGNPKATVMFALRSLCGLAVVFSFISPLVLETFVEPSVAGVYARLG
HHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
LKTEGFIPGASLTGGAGYFWIYLLFILLGLGAWIAWKAARKVSNSAHAQPYFSGLTQEQA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHC
GQIGFKGPMNAFEPVRLSNFYLTQYFGEGTITRAIDIISTAFLIVLVGGLL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]