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The map label for this gene is nqo13 [H]

Identifier: 220904921

GI number: 220904921

Start: 1992682

End: 1994169

Strand: Reverse

Name: nqo13 [H]

Synonym: Ddes_1656

Alternate gene names: 220904921

Gene position: 1994169-1992682 (Counterclockwise)

Preceding gene: 220904922

Following gene: 220904920

Centisome position: 69.4

GC content: 56.32

Gene sequence:

>1488_bases
ATGTCCGTACCCGTACTTTCCTTGCTTATCTTTGTTCCTGTGCTTGGCGGTCTCTCGCTTATGCTCCTGCCCAGAGGGCG
GGAGGAGACTATCAGGCTGGGGGCGCTGACCGTCTGTGCCGTGGAACTGGCCCTGAGCCTCTGGGCGCTCAGCCAGTTTG
ACAAGGGCTTGTGGCAGATGCAGATGGTGGAAAACCGCCCCTGGATTGAAAGCCTGAACATACGCTACAGCTTGGGCATT
GACGGCATAAGTGTGCTCTTTTTGCCGCTGACGGCGCTCATCACGCTTATGGGCGTGGTGGTGTCGTTTAAAAGCATCAC
CCTGAAGGTCAAGGAATTCTTCGTCAGCCTGCTGCTGCTCGAGGGGGCCATGATAGGCGTGTTCTGCGCGCTGGACCTCA
TGCTTTTTTATGTGTTCTGGGAGGCCATGCTCATTCCGATGTTTCTGCTCATCGGTGTGTGGGGAGGGCCTCGGCGCATC
TATGCCACGGTAAAGTTTTTCCTTTACACCCTGTTGGGCAGCCTGCTCATGCTTGTGGGCATCATTCTTCTTTATCTCAA
GGGCGGCAATACCTTCGACATGCTGGCCCTGGCCGGGCAGAACTACAGCAAAGACCTGCAATTACTTTTATTCTGGGCCT
TTTTTGCCGCATTTGCCGTCAAGGTGCCCATGTGGCCCGTGCACACCTGGCTGCCTGATGCCCATACTGAAGCACCCACG
GCAGGCTCGGTCATTCTGGCGGGGGTACTGATCAAAATGGGGGCTTACGGCTTTTTACGCATCAGTCTGCCCATTCTGCC
TTACGCCGCCTGGACCCTGTTGACGCCCATGCTGATTCTTTCTGTTATCGCCATCGTTTACGGCGCTCTGGTCTGCCTGG
CGCAAACCGATATCAAGCGTCTGATCGCATACAGTTCTGTGAGCCATATGGGTTTTGTTACCCTGGGACTTTTCGCCCTG
ACACAGAAGGGTGTTGAAGGTTCCATCATCCAGATGCTCAACCACGGTATCGTGACAGGGGCGCTTTTTCTGGCCGTGGG
CATGATCTATGACCGCACCCATACACGTCAGATACGCGACTACGGCGGCCTTGCCACCACCATGCCCGTCATGGCCGGAT
TTTTTCTGGTGTTCACCCTGGCAGCCATCGGTCTGCCGGGCACTAACGGCTTTATCGGCGAGTTCCTTATTCTGCTGGGC
GGATTCGAGCGCGCACCCTGGGCTGCGGTGGTCGCGTCCTCGGGGCTTATTCTGGGGGCCTGGTACATGCTGTGGCTCTA
CCAGCGAGTGTTTTTCAAGCAGGTTTCAGATACGGTGCGTGGTTTGCCCGGTCTTGACGGCAGAGAGCTGAGCATCATGC
TGACCATGGCCGTGTTGGTGCTCGCCATAGGCATTTTCCCCAATATGCTGCTGGAATACACCCACGCTTCAGTAGAGCGG
CTTGTCAGTGACACGCAGCAGGTTTTTGCGGCTTTTGCAGCCCGGTAG

Upstream 100 bases:

>100_bases
GCATGTGGCCCATTATCTGGCCTGGCTGGCCGGAGCCTCGGTGGTGCTTCTGATTGTGCTGCAACTGGGAATTTTCTCGT
AAACACGGCAGGGAGACAGC

Downstream 100 bases:

>100_bases
GGCGGAGTAACAAGGAGCCACCATGACTGATACTGTTATTGCGCCTCTTTCTGCACTTCCCTTGCTGGCCGATCTGCCCG
CCCTTGCGCCGGAGCTGGTT

Product: proton-translocating NADH-quinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSVPVLSLLIFVPVLGGLSLMLLPRGREETIRLGALTVCAVELALSLWALSQFDKGLWQMQMVENRPWIESLNIRYSLGI
DGISVLFLPLTALITLMGVVVSFKSITLKVKEFFVSLLLLEGAMIGVFCALDLMLFYVFWEAMLIPMFLLIGVWGGPRRI
YATVKFFLYTLLGSLLMLVGIILLYLKGGNTFDMLALAGQNYSKDLQLLLFWAFFAAFAVKVPMWPVHTWLPDAHTEAPT
AGSVILAGVLIKMGAYGFLRISLPILPYAAWTLLTPMLILSVIAIVYGALVCLAQTDIKRLIAYSSVSHMGFVTLGLFAL
TQKGVEGSIIQMLNHGIVTGALFLAVGMIYDRTHTRQIRDYGGLATTMPVMAGFFLVFTLAAIGLPGTNGFIGEFLILLG
GFERAPWAAVVASSGLILGAWYMLWLYQRVFFKQVSDTVRGLPGLDGRELSIMLTMAVLVLAIGIFPNMLLEYTHASVER
LVSDTQQVFAAFAAR

Sequences:

>Translated_495_residues
MSVPVLSLLIFVPVLGGLSLMLLPRGREETIRLGALTVCAVELALSLWALSQFDKGLWQMQMVENRPWIESLNIRYSLGI
DGISVLFLPLTALITLMGVVVSFKSITLKVKEFFVSLLLLEGAMIGVFCALDLMLFYVFWEAMLIPMFLLIGVWGGPRRI
YATVKFFLYTLLGSLLMLVGIILLYLKGGNTFDMLALAGQNYSKDLQLLLFWAFFAAFAVKVPMWPVHTWLPDAHTEAPT
AGSVILAGVLIKMGAYGFLRISLPILPYAAWTLLTPMLILSVIAIVYGALVCLAQTDIKRLIAYSSVSHMGFVTLGLFAL
TQKGVEGSIIQMLNHGIVTGALFLAVGMIYDRTHTRQIRDYGGLATTMPVMAGFFLVFTLAAIGLPGTNGFIGEFLILLG
GFERAPWAAVVASSGLILGAWYMLWLYQRVFFKQVSDTVRGLPGLDGRELSIMLTMAVLVLAIGIFPNMLLEYTHASVER
LVSDTQQVFAAFAAR
>Mature_494_residues
SVPVLSLLIFVPVLGGLSLMLLPRGREETIRLGALTVCAVELALSLWALSQFDKGLWQMQMVENRPWIESLNIRYSLGID
GISVLFLPLTALITLMGVVVSFKSITLKVKEFFVSLLLLEGAMIGVFCALDLMLFYVFWEAMLIPMFLLIGVWGGPRRIY
ATVKFFLYTLLGSLLMLVGIILLYLKGGNTFDMLALAGQNYSKDLQLLLFWAFFAAFAVKVPMWPVHTWLPDAHTEAPTA
GSVILAGVLIKMGAYGFLRISLPILPYAAWTLLTPMLILSVIAIVYGALVCLAQTDIKRLIAYSSVSHMGFVTLGLFALT
QKGVEGSIIQMLNHGIVTGALFLAVGMIYDRTHTRQIRDYGGLATTMPVMAGFFLVFTLAAIGLPGTNGFIGEFLILLGG
FERAPWAAVVASSGLILGAWYMLWLYQRVFFKQVSDTVRGLPGLDGRELSIMLTMAVLVLAIGIFPNMLLEYTHASVERL
VSDTQQVFAAFAAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=370, Percent_Identity=33.7837837837838, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI251831117, Length=372, Percent_Identity=26.3440860215054, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1788613, Length=504, Percent_Identity=40.2777777777778, Blast_Score=337, Evalue=2e-93,
Organism=Escherichia coli, GI1788827, Length=296, Percent_Identity=30.0675675675676, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788831, Length=347, Percent_Identity=27.6657060518732, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788829, Length=477, Percent_Identity=26.8343815513627, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1788614, Length=418, Percent_Identity=27.7511961722488, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI145693160, Length=435, Percent_Identity=27.1264367816092, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI2367154, Length=198, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54365; Mature: 54234

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPVLSLLIFVPVLGGLSLMLLPRGREETIRLGALTVCAVELALSLWALSQFDKGLWQM
CCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMVENRPWIESLNIRYSLGIDGISVLFLPLTALITLMGVVVSFKSITLKVKEFFVSLLLL
HHHCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGAMIGVFCALDLMLFYVFWEAMLIPMFLLIGVWGGPRRIYATVKFFLYTLLGSLLMLVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IILLYLKGGNTFDMLALAGQNYSKDLQLLLFWAFFAAFAVKVPMWPVHTWLPDAHTEAPT
HHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCC
AGSVILAGVLIKMGAYGFLRISLPILPYAAWTLLTPMLILSVIAIVYGALVCLAQTDIKR
CHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIAYSSVSHMGFVTLGLFALTQKGVEGSIIQMLNHGIVTGALFLAVGMIYDRTHTRQIRD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
YGGLATTMPVMAGFFLVFTLAAIGLPGTNGFIGEFLILLGGFERAPWAAVVASSGLILGA
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCHHHHH
WYMLWLYQRVFFKQVSDTVRGLPGLDGRELSIMLTMAVLVLAIGIFPNMLLEYTHASVER
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVSDTQQVFAAFAAR
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVPVLSLLIFVPVLGGLSLMLLPRGREETIRLGALTVCAVELALSLWALSQFDKGLWQM
CCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMVENRPWIESLNIRYSLGIDGISVLFLPLTALITLMGVVVSFKSITLKVKEFFVSLLLL
HHHCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGAMIGVFCALDLMLFYVFWEAMLIPMFLLIGVWGGPRRIYATVKFFLYTLLGSLLMLVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IILLYLKGGNTFDMLALAGQNYSKDLQLLLFWAFFAAFAVKVPMWPVHTWLPDAHTEAPT
HHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCC
AGSVILAGVLIKMGAYGFLRISLPILPYAAWTLLTPMLILSVIAIVYGALVCLAQTDIKR
CHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIAYSSVSHMGFVTLGLFALTQKGVEGSIIQMLNHGIVTGALFLAVGMIYDRTHTRQIRD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
YGGLATTMPVMAGFFLVFTLAAIGLPGTNGFIGEFLILLGGFERAPWAAVVASSGLILGA
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCHHHHH
WYMLWLYQRVFFKQVSDTVRGLPGLDGRELSIMLTMAVLVLAIGIFPNMLLEYTHASVER
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVSDTQQVFAAFAAR
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]