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The map label for this gene is glyA

Identifier: 220904882

GI number: 220904882

Start: 1941675

End: 1942919

Strand: Direct

Name: glyA

Synonym: Ddes_1617

Alternate gene names: 220904882

Gene position: 1941675-1942919 (Clockwise)

Preceding gene: 220904881

Following gene: 220904883

Centisome position: 67.57

GC content: 58.63

Gene sequence:

>1245_bases
ATGGATGAAATTCTGCTGCAAGACCCGGAAATCGCAAAGGCCATTGCTCTGGAATCGCAAAGGCAGATGGGCAAGCTTGA
GCTTATCGCCTCGGAAAACATTGTTTCCACGGCGGTGCGCGAAGCCCAGGGCAGCGTACTGACCAACAAATACGCCGAAG
GCTATCCCGGCAAGCGCTACTACGGCGGCTGCGAATACGTGGATATGGTGGAAACCCTGGCGCAGGAAAGAGCCAAGCTG
CTTTTTGACGCACAATACGTCAACGTGCAGCCCCACTCCGGCTCACAGGCCAACATGGCCGCCTACCTGGCAGTTCTCAA
ACCCGGCGACACCATTCTTGGCATGGACCTTTCCCACGGCGGGCACCTCACTCACGGCAGCCCCGTGAACTTTTCCGGCC
GTCTTTTCAAGATCATCTCGTATGGGGTGCAGCGCGAAACCGGGCGCATCGACTATGACGATGTGGCCGCAAAAGCCCGC
GAACACAAGCCCAGTGTCATTGTTGCCGGCGCCAGCGCCTACCCCCGCGCCATTGATTTTGCCCGTTTTCGCGCCATTGC
CGATGAGGTGGGCGCAAAGCTTGTGGTGGACATGGCCCACATCGCGGGTCTCGTGGCTGCAGGTCTGCACCAAAGCCCGG
TTCCGCATGCCCATATCACCACCACAACCACGCACAAAACCCTGCGCGGTCCGCGCGGCGGCATGATACTGTCCACGGAA
GATATGGGCAAAACCCTGAACAGCCAGATTTTCCCCGGCATTCAGGGCGGTCCCCTCATGCATGTCATTGCCGCCAAGGC
CGTGGCCCTGGGCGAAGCCCTGCACCCTGCCTTCAAGGTGTATCAGCAGCAGGTGCTGGATAACGCCGCAACCCTGGCCG
CATGCCTTACGGAAGCGGGTTACGACCTTGTCTCCGGCGGTACGGACAACCACCTCATGCTGGTTGACCTGACCAGCAGG
GATATTACCGGCAAGGACGCTGAAATTGCCCTGGATACGGCGGGCATCACGGTCAATAAAAACACCGTGCCCTTTGAAAC
GCGCTCGCCCTTCGTTACGTCCGGCATACGCCTGGGCACTGCCGCCCTTACCACGCGCGGCATGAAGCAGGAGCACATGC
GCACTGTGGGGCAGTTTATCATTGCAGCGCTTGAAAAACGGAACGACACTGCGGAACTTGAAAAAATTCGTAAAAACGTT
GAAGAATTCGCACATCAGTTCCCCCTGTTCGCCCACCTTGCCTGA

Upstream 100 bases:

>100_bases
TAACAGGCCGCCGGACGCTGTACTCTCCGCCGGGCCAGCCCAGTACACACACTGCCCGCCACTGTGGCGGGCAGTATATT
TCACTAAGGATGCCAAGTTC

Downstream 100 bases:

>100_bases
AAACATTTGCAAGGTTGCCCTTTTCAAAGGTTTTCAGATCTCCGGGCGTTCAGTTGACAGGTAAAAAATATCCGGGCCTT
GTAAAGCATCCGCTCTGCAA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MDEILLQDPEIAKAIALESQRQMGKLELIASENIVSTAVREAQGSVLTNKYAEGYPGKRYYGGCEYVDMVETLAQERAKL
LFDAQYVNVQPHSGSQANMAAYLAVLKPGDTILGMDLSHGGHLTHGSPVNFSGRLFKIISYGVQRETGRIDYDDVAAKAR
EHKPSVIVAGASAYPRAIDFARFRAIADEVGAKLVVDMAHIAGLVAAGLHQSPVPHAHITTTTTHKTLRGPRGGMILSTE
DMGKTLNSQIFPGIQGGPLMHVIAAKAVALGEALHPAFKVYQQQVLDNAATLAACLTEAGYDLVSGGTDNHLMLVDLTSR
DITGKDAEIALDTAGITVNKNTVPFETRSPFVTSGIRLGTAALTTRGMKQEHMRTVGQFIIAALEKRNDTAELEKIRKNV
EEFAHQFPLFAHLA

Sequences:

>Translated_414_residues
MDEILLQDPEIAKAIALESQRQMGKLELIASENIVSTAVREAQGSVLTNKYAEGYPGKRYYGGCEYVDMVETLAQERAKL
LFDAQYVNVQPHSGSQANMAAYLAVLKPGDTILGMDLSHGGHLTHGSPVNFSGRLFKIISYGVQRETGRIDYDDVAAKAR
EHKPSVIVAGASAYPRAIDFARFRAIADEVGAKLVVDMAHIAGLVAAGLHQSPVPHAHITTTTTHKTLRGPRGGMILSTE
DMGKTLNSQIFPGIQGGPLMHVIAAKAVALGEALHPAFKVYQQQVLDNAATLAACLTEAGYDLVSGGTDNHLMLVDLTSR
DITGKDAEIALDTAGITVNKNTVPFETRSPFVTSGIRLGTAALTTRGMKQEHMRTVGQFIIAALEKRNDTAELEKIRKNV
EEFAHQFPLFAHLA
>Mature_414_residues
MDEILLQDPEIAKAIALESQRQMGKLELIASENIVSTAVREAQGSVLTNKYAEGYPGKRYYGGCEYVDMVETLAQERAKL
LFDAQYVNVQPHSGSQANMAAYLAVLKPGDTILGMDLSHGGHLTHGSPVNFSGRLFKIISYGVQRETGRIDYDDVAAKAR
EHKPSVIVAGASAYPRAIDFARFRAIADEVGAKLVVDMAHIAGLVAAGLHQSPVPHAHITTTTTHKTLRGPRGGMILSTE
DMGKTLNSQIFPGIQGGPLMHVIAAKAVALGEALHPAFKVYQQQVLDNAATLAACLTEAGYDLVSGGTDNHLMLVDLTSR
DITGKDAEIALDTAGITVNKNTVPFETRSPFVTSGIRLGTAALTTRGMKQEHMRTVGQFIIAALEKRNDTAELEKIRKNV
EEFAHQFPLFAHLA

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=447, Percent_Identity=47.427293064877, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI261862352, Length=447, Percent_Identity=47.427293064877, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI261862350, Length=447, Percent_Identity=47.427293064877, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI261862348, Length=447, Percent_Identity=47.427293064877, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI261862346, Length=447, Percent_Identity=46.3087248322148, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI22547186, Length=460, Percent_Identity=45.8695652173913, Blast_Score=347, Evalue=8e-96,
Organism=Homo sapiens, GI22547189, Length=445, Percent_Identity=44.7191011235955, Blast_Score=318, Evalue=8e-87,
Organism=Escherichia coli, GI1788902, Length=407, Percent_Identity=59.7051597051597, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI25144732, Length=427, Percent_Identity=45.9016393442623, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=427, Percent_Identity=46.135831381733, Blast_Score=345, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=415, Percent_Identity=45.3012048192771, Blast_Score=354, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=45.771144278607, Blast_Score=337, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24640005, Length=460, Percent_Identity=47.1739130434783, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI221329721, Length=460, Percent_Identity=47.1739130434783, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_DESDA (B8J189)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480194.1
- ProteinModelPortal:   B8J189
- GeneID:   7285316
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1617
- HOGENOM:   HBG301263
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 44638; Mature: 44638

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEILLQDPEIAKAIALESQRQMGKLELIASENIVSTAVREAQGSVLTNKYAEGYPGKRY
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCCC
YGGCEYVDMVETLAQERAKLLFDAQYVNVQPHSGSQANMAAYLAVLKPGDTILGMDLSHG
CCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEEECCC
GHLTHGSPVNFSGRLFKIISYGVQRETGRIDYDDVAAKAREHKPSVIVAGASAYPRAIDF
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHH
ARFRAIADEVGAKLVVDMAHIAGLVAAGLHQSPVPHAHITTTTTHKTLRGPRGGMILSTE
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHCCCCCCEEEECH
DMGKTLNSQIFPGIQGGPLMHVIAAKAVALGEALHPAFKVYQQQVLDNAATLAACLTEAG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YDLVSGGTDNHLMLVDLTSRDITGKDAEIALDTAGITVNKNTVPFETRSPFVTSGIRLGT
CHHHCCCCCCEEEEEEECCCCCCCCCCEEEEEECCEEECCCCCCCCCCCCCHHCCCCHHH
AALTTRGMKQEHMRTVGQFIIAALEKRNDTAELEKIRKNVEEFAHQFPLFAHLA
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MDEILLQDPEIAKAIALESQRQMGKLELIASENIVSTAVREAQGSVLTNKYAEGYPGKRY
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCCC
YGGCEYVDMVETLAQERAKLLFDAQYVNVQPHSGSQANMAAYLAVLKPGDTILGMDLSHG
CCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEEECCC
GHLTHGSPVNFSGRLFKIISYGVQRETGRIDYDDVAAKAREHKPSVIVAGASAYPRAIDF
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHH
ARFRAIADEVGAKLVVDMAHIAGLVAAGLHQSPVPHAHITTTTTHKTLRGPRGGMILSTE
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHCCCCCCEEEECH
DMGKTLNSQIFPGIQGGPLMHVIAAKAVALGEALHPAFKVYQQQVLDNAATLAACLTEAG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YDLVSGGTDNHLMLVDLTSRDITGKDAEIALDTAGITVNKNTVPFETRSPFVTSGIRLGT
CHHHCCCCCCEEEEEEECCCCCCCCCCEEEEEECCEEECCCCCCCCCCCCCHHCCCCHHH
AALTTRGMKQEHMRTVGQFIIAALEKRNDTAELEKIRKNVEEFAHQFPLFAHLA
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA