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The map label for this gene is groEL

Identifier: 220904816

GI number: 220904816

Start: 1854361

End: 1856010

Strand: Direct

Name: groEL

Synonym: Ddes_1548

Alternate gene names: 220904816

Gene position: 1854361-1856010 (Clockwise)

Preceding gene: 220904815

Following gene: 220904817

Centisome position: 64.53

GC content: 58.91

Gene sequence:

>1650_bases
ATGTCTGCTAAAGAAATCTTTTTTGACGTTAAAGCCCGCGAAAAACTTTCCCGCGGTGTTGACAAGCTTGCCAATGCCGT
CAAGGTCACCCTTGGCCCCAAAGGCCGCAACGTGGCCATTGAAAAATCTTTTGGCGCGCCCGTCATTACCAAAGACGGCG
TGACTGTCGCCAAGGAAATCGAACTGTCCGACAAGTTCGAAAACATGGGTGCCCAGCTCGTCAAAGAAGTGGCCTCCAAA
ACTTCCGACGCCGCCGGTGACGGTACCACCACCGCCACCATCCTGGCTCAGGCCATCTATCGCGAAGGCGTCAAGCTTGT
GGCTGCCGGGCGCAATCCCATGGCCATCAAGCGCGGCATCGACAAGGCTGTTGAAGGCCTGATCACCGAACTTGCCAACC
TTGCCAAACCCACCCGCGACCAGAAGGAAATTGCCCAGATCGGCACCATTTCCGCCAACTCCGATACCACCATCGGCAAC
ATCATTGCTGAAGCCATGGCCAAGGTGGGCAAGGAAGGCGTCATCACGGTTGAAGAAGCCAAGGGCCTTGAAACCACTAT
GGACGTGGTGGAAGGCATGCGCTTTGACCGCGGCTACCTGTCGCCCTACTTCGTGACCAATCCCGAAAAGATGGTCTGCG
AAATGGACAATCCTTACATCCTGTGCACGGAAAAGAAAATCTCCAGCATGAAAGACATGCTGCCCGTGCTTGAGCAGGTT
GCCAAGGTGAACCGCCCGCTCATGATCATCGCTGAAGACGTGGAAGGCGAAGCCCTGGCCACCCTGGTGGTCAACAAGCT
GCGTGGTGCCCTGCAGGTCGTGGCTGTCAAGGCCCCCGGCTTTGGCGACCGCCGCAAGGCCATGCTGCAGGATATCGCCG
TGCTCACCGGCGGCCAGGTGGCTTCCGACGACACCGGCTCCAAGCTTGAAAACATGACCCTGGCCGAACTCGGTACCGCC
AAGCGTATCGTGATCGACAAGGAAAACACCACGGTTGTGGACGGCGCCGGCAAGAGCGAAGACATCAAGGCTCGCGTGAA
GCAGATCCGCGCCCAGATCGAAGACAGCACCTCTGACTACGACCGCGAAAAGCTGCAGGAACGCCTTGCCAAGCTGGTGG
GCGGCGTGGCCGTGGTGCATGTGGGCGCTGCCACCGAAGTGGAAATGAAAGAAAAGAAAGACCGCGTTGAAGACGCCCTC
AATGCCACCCGCGCTGCCGTGGAAGAAGGCATTGTGCCTGGCGGCGGTACCGCTCTTATCCGCGTTGCCAAGGTGCTTTG
CGACATCAAGCCCGCTGACGACGACGAACTGGCCGGTGTGAATATCATTCGCCGCGCCATTGAAGAGCCTCTGCGCCAGA
TCGCCCTCAATGCCGGCTTTGAAGGCTCCATCGTGGTCGAAAAAGTGCGCGAAGGCAAAGACGGCTTCGGCTTCAACGCC
GCCACCGGCGAATACGAAGACCTGATCAAGGCCGGCGTGATCGACCCCAAAAAGGTGACCCGCACTGCCCTGCAGAACGC
CGCTTCCGTGGCTTCCCTGCTGCTGACCACCGAATGCGCCATTGCCGAAAAGCCCGAACCCAAGAAAGACGCCGCCATGC
CTGACATGGGCGGTATGGGTGGCATGGGCGGCATGGGCGGCATGTACTAA

Upstream 100 bases:

>100_bases
TCGACGGCGTTGACCATCTGGTCATGCGCGAAGAAGATATCCTCGCCATCATCGACTAACTCCCACCTTTGCCGAATTAC
CACTAGGAGGAAATGTCCCA

Downstream 100 bases:

>100_bases
GGCCGGTCCCTGCAGTTTAAACGTCTCTTATCAAAGCCCCCGCGCAAGCGGGGGCTTTTTTCGCAACAGGAGCATACCGT
GATACGCTATGGCTTTGCAT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MSAKEIFFDVKAREKLSRGVDKLANAVKVTLGPKGRNVAIEKSFGAPVITKDGVTVAKEIELSDKFENMGAQLVKEVASK
TSDAAGDGTTTATILAQAIYREGVKLVAAGRNPMAIKRGIDKAVEGLITELANLAKPTRDQKEIAQIGTISANSDTTIGN
IIAEAMAKVGKEGVITVEEAKGLETTMDVVEGMRFDRGYLSPYFVTNPEKMVCEMDNPYILCTEKKISSMKDMLPVLEQV
AKVNRPLMIIAEDVEGEALATLVVNKLRGALQVVAVKAPGFGDRRKAMLQDIAVLTGGQVASDDTGSKLENMTLAELGTA
KRIVIDKENTTVVDGAGKSEDIKARVKQIRAQIEDSTSDYDREKLQERLAKLVGGVAVVHVGAATEVEMKEKKDRVEDAL
NATRAAVEEGIVPGGGTALIRVAKVLCDIKPADDDELAGVNIIRRAIEEPLRQIALNAGFEGSIVVEKVREGKDGFGFNA
ATGEYEDLIKAGVIDPKKVTRTALQNAASVASLLLTTECAIAEKPEPKKDAAMPDMGGMGGMGGMGGMY

Sequences:

>Translated_549_residues
MSAKEIFFDVKAREKLSRGVDKLANAVKVTLGPKGRNVAIEKSFGAPVITKDGVTVAKEIELSDKFENMGAQLVKEVASK
TSDAAGDGTTTATILAQAIYREGVKLVAAGRNPMAIKRGIDKAVEGLITELANLAKPTRDQKEIAQIGTISANSDTTIGN
IIAEAMAKVGKEGVITVEEAKGLETTMDVVEGMRFDRGYLSPYFVTNPEKMVCEMDNPYILCTEKKISSMKDMLPVLEQV
AKVNRPLMIIAEDVEGEALATLVVNKLRGALQVVAVKAPGFGDRRKAMLQDIAVLTGGQVASDDTGSKLENMTLAELGTA
KRIVIDKENTTVVDGAGKSEDIKARVKQIRAQIEDSTSDYDREKLQERLAKLVGGVAVVHVGAATEVEMKEKKDRVEDAL
NATRAAVEEGIVPGGGTALIRVAKVLCDIKPADDDELAGVNIIRRAIEEPLRQIALNAGFEGSIVVEKVREGKDGFGFNA
ATGEYEDLIKAGVIDPKKVTRTALQNAASVASLLLTTECAIAEKPEPKKDAAMPDMGGMGGMGGMGGMY
>Mature_548_residues
SAKEIFFDVKAREKLSRGVDKLANAVKVTLGPKGRNVAIEKSFGAPVITKDGVTVAKEIELSDKFENMGAQLVKEVASKT
SDAAGDGTTTATILAQAIYREGVKLVAAGRNPMAIKRGIDKAVEGLITELANLAKPTRDQKEIAQIGTISANSDTTIGNI
IAEAMAKVGKEGVITVEEAKGLETTMDVVEGMRFDRGYLSPYFVTNPEKMVCEMDNPYILCTEKKISSMKDMLPVLEQVA
KVNRPLMIIAEDVEGEALATLVVNKLRGALQVVAVKAPGFGDRRKAMLQDIAVLTGGQVASDDTGSKLENMTLAELGTAK
RIVIDKENTTVVDGAGKSEDIKARVKQIRAQIEDSTSDYDREKLQERLAKLVGGVAVVHVGAATEVEMKEKKDRVEDALN
ATRAAVEEGIVPGGGTALIRVAKVLCDIKPADDDELAGVNIIRRAIEEPLRQIALNAGFEGSIVVEKVREGKDGFGFNAA
TGEYEDLIKAGVIDPKKVTRTALQNAASVASLLLTTECAIAEKPEPKKDAAMPDMGGMGGMGGMGGMY

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=529, Percent_Identity=50.6616257088847, Blast_Score=528, Evalue=1e-150,
Organism=Homo sapiens, GI31542947, Length=529, Percent_Identity=50.6616257088847, Blast_Score=528, Evalue=1e-150,
Organism=Homo sapiens, GI5453607, Length=536, Percent_Identity=23.5074626865672, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790586, Length=532, Percent_Identity=65.7894736842105, Blast_Score=689, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=526, Percent_Identity=50.9505703422053, Blast_Score=531, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI193210679, Length=208, Percent_Identity=50.4807692307692, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17564182, Length=549, Percent_Identity=21.6757741347905, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=55.5133079847909, Blast_Score=556, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6322350, Length=552, Percent_Identity=25.3623188405797, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24641193, Length=525, Percent_Identity=53.1428571428571, Blast_Score=547, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24641191, Length=525, Percent_Identity=53.1428571428571, Blast_Score=547, Evalue=1e-156,
Organism=Drosophila melanogaster, GI45550936, Length=524, Percent_Identity=51.1450381679389, Blast_Score=520, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45550132, Length=524, Percent_Identity=51.1450381679389, Blast_Score=520, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45550935, Length=524, Percent_Identity=51.1450381679389, Blast_Score=520, Evalue=1e-147,
Organism=Drosophila melanogaster, GI17864606, Length=543, Percent_Identity=45.3038674033149, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24584129, Length=546, Percent_Identity=36.2637362637363, Blast_Score=338, Evalue=5e-93,
Organism=Drosophila melanogaster, GI19921262, Length=546, Percent_Identity=36.2637362637363, Blast_Score=338, Evalue=5e-93,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_DESDA (B8J123)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480128.1
- GeneID:   7285246
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1548
- HOGENOM:   HBG625289
- ProtClustDB:   PRK00013
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 58419; Mature: 58288

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKEIFFDVKAREKLSRGVDKLANAVKVTLGPKGRNVAIEKSFGAPVITKDGVTVAKEI
CCCHHHEEEHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCEEECCCCEEEHHC
ELSDKFENMGAQLVKEVASKTSDAAGDGTTTATILAQAIYREGVKLVAAGRNPMAIKRGI
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
DKAVEGLITELANLAKPTRDQKEIAQIGTISANSDTTIGNIIAEAMAKVGKEGVITVEEA
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCC
KGLETTMDVVEGMRFDRGYLSPYFVTNPEKMVCEMDNPYILCTEKKISSMKDMLPVLEQV
CCHHHHHHHHHCCCCCCCCCCCEEECCCHHEEEECCCCEEEEEHHHHHHHHHHHHHHHHH
AKVNRPLMIIAEDVEGEALATLVVNKLRGALQVVAVKAPGFGDRRKAMLQDIAVLTGGQV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCC
ASDDTGSKLENMTLAELGTAKRIVIDKENTTVVDGAGKSEDIKARVKQIRAQIEDSTSDY
CCCCCCCHHHCCHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
DREKLQERLAKLVGGVAVVHVGAATEVEMKEKKDRVEDALNATRAAVEEGIVPGGGTALI
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RVAKVLCDIKPADDDELAGVNIIRRAIEEPLRQIALNAGFEGSIVVEKVREGKDGFGFNA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCC
ATGEYEDLIKAGVIDPKKVTRTALQNAASVASLLLTTECAIAEKPEPKKDAAMPDMGGMG
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GMGGMGGMY
CCCCCCCCC
>Mature Secondary Structure 
SAKEIFFDVKAREKLSRGVDKLANAVKVTLGPKGRNVAIEKSFGAPVITKDGVTVAKEI
CCHHHEEEHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCEEECCCCEEEHHC
ELSDKFENMGAQLVKEVASKTSDAAGDGTTTATILAQAIYREGVKLVAAGRNPMAIKRGI
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
DKAVEGLITELANLAKPTRDQKEIAQIGTISANSDTTIGNIIAEAMAKVGKEGVITVEEA
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCC
KGLETTMDVVEGMRFDRGYLSPYFVTNPEKMVCEMDNPYILCTEKKISSMKDMLPVLEQV
CCHHHHHHHHHCCCCCCCCCCCEEECCCHHEEEECCCCEEEEEHHHHHHHHHHHHHHHHH
AKVNRPLMIIAEDVEGEALATLVVNKLRGALQVVAVKAPGFGDRRKAMLQDIAVLTGGQV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCC
ASDDTGSKLENMTLAELGTAKRIVIDKENTTVVDGAGKSEDIKARVKQIRAQIEDSTSDY
CCCCCCCHHHCCHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
DREKLQERLAKLVGGVAVVHVGAATEVEMKEKKDRVEDALNATRAAVEEGIVPGGGTALI
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RVAKVLCDIKPADDDELAGVNIIRRAIEEPLRQIALNAGFEGSIVVEKVREGKDGFGFNA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCC
ATGEYEDLIKAGVIDPKKVTRTALQNAASVASLLLTTECAIAEKPEPKKDAAMPDMGGMG
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GMGGMGGMY
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA