Click here to switch to the map view.

The map label for this gene is rho [H]

Identifier: 220904724

GI number: 220904724

Start: 1744049

End: 1745344

Strand: Direct

Name: rho [H]

Synonym: Ddes_1456

Alternate gene names: 220904724

Gene position: 1744049-1745344 (Clockwise)

Preceding gene: 220904723

Following gene: 220904725

Centisome position: 60.7

GC content: 55.71

Gene sequence:

>1296_bases
ATGCGCAAAAAGAAAGCTACCCCCACACTGTTGACAGACAGCGCCCTGAGCCTCACTGACCTGAAGACCCGCAGCATGCA
GGAACTCATGGATCTGGCTGAACAGTACGAGATTGAGAATGCCAGTTCCATGCGAAAGCAAGAGCTTATCTTCGCACTGC
TTTCCACCTGCGCCTCGCAGAACGGTGCCATCTACGGCGACGGCGTGCTGGAGATACTGCCGGATGGCTTTGGCTTTCTG
CGCTCGCCCCTTTGCAGCTACATGCCGGGACCAGACGACATATATGTGTCGCCATCGCAAATCCGACGCTTCTCCCTGCG
TAAAGGTGACATTGTTTCCGGGCAGATCCGCCCGCCCAAGGAAGGGGAGCGCTACTTCGCCCTGCTGAAGGTTACCGAGA
TCGGCTTTGAACCGCCGGAACATGCCAAAAATCTCGTCCTGTTTGACAACCTTACCCCCATCTACCCCGACCATCAGCTC
GTCATGGAAAATGGCGAGAAAAATCTTTCCAACCGCGTCATCGACATCATGGCCCCCATTGGTCGTGGTCAGCGCGGCCT
TATTGTTGCCCCCCCCCGCACAGGCAAAACCATTCTGCTCCAATCGCTTGCCAATGCCATCAACGCCAATAATCCGGAAG
TCTACCTTATCGTGCTGCTGATTGACGAGCGCCCCGAGGAAGTGACGGATATGGAACGTACGGTCAAAAAGGCCGAAGTA
ATCAGTTCCACGTTTGACGAGCCGCCGCAGCGCCATGTGCAGGTCTGTGAAATGGTGCTTGAAAAGGCCAAACGCCTTGT
AGAACGCAAGCGCGATGTTGTTATCCTGCTTGACTCGATCACCCGCCTTGGCAGGGCCTACAACGCCGTAACCCCCTCGT
CCGGGCGTGTACTTTCCGGTGGGCTGGACGCCAACGCCCTGCAGCGGCCCAAACGCTTTTTCGGTGCGGCCCGTAACATC
GAAGAAGGCGGCAGCCTGACCATTATCGCTACCGCCCTGATTGATACCGGTTCACGCATGGACGAAGTAATCTTTGAAGA
GTTCAAGGGTACAGGCAATATGGAAATCTACCTGGACCGCCATCTTTCGGAAAAGCGCGTGTTCCCTGCCATCGATATCA
ACCGCACCGGCACCCGCAAGGAAGACCTGCTGCTGCCCGAAGACGTGCTCAACCGCGTCTGGATACTGCGCAAGATTCTG
GCCCCCATGTCTGCCATTGACAGTATGGAATTCCTGCTGGACAAGATGCGCGGCACCAAATCCAATAAAGACTTTATGAA
CGCCATGGGCAAATAG

Upstream 100 bases:

>100_bases
ACGCACACCTATTTTGTCTCCTTACATCCAATCGCCAATAGTACAGTGTTGTAAAATACTGTATGGCTTTAGTTTGTCTT
ATTCAAAAGATCGGACAGTT

Downstream 100 bases:

>100_bases
GCCTGTTCAAACAAAGCCTGCAAATGCCTTATTGAACCCCGGTTTTCCGGGGTTTTTTATTTATTGCTGTTAGCATTTAA
AATCACTGCTTTTTCCCAAA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MRKKKATPTLLTDSALSLTDLKTRSMQELMDLAEQYEIENASSMRKQELIFALLSTCASQNGAIYGDGVLEILPDGFGFL
RSPLCSYMPGPDDIYVSPSQIRRFSLRKGDIVSGQIRPPKEGERYFALLKVTEIGFEPPEHAKNLVLFDNLTPIYPDHQL
VMENGEKNLSNRVIDIMAPIGRGQRGLIVAPPRTGKTILLQSLANAINANNPEVYLIVLLIDERPEEVTDMERTVKKAEV
ISSTFDEPPQRHVQVCEMVLEKAKRLVERKRDVVILLDSITRLGRAYNAVTPSSGRVLSGGLDANALQRPKRFFGAARNI
EEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEIYLDRHLSEKRVFPAIDINRTGTRKEDLLLPEDVLNRVWILRKIL
APMSAIDSMEFLLDKMRGTKSNKDFMNAMGK

Sequences:

>Translated_431_residues
MRKKKATPTLLTDSALSLTDLKTRSMQELMDLAEQYEIENASSMRKQELIFALLSTCASQNGAIYGDGVLEILPDGFGFL
RSPLCSYMPGPDDIYVSPSQIRRFSLRKGDIVSGQIRPPKEGERYFALLKVTEIGFEPPEHAKNLVLFDNLTPIYPDHQL
VMENGEKNLSNRVIDIMAPIGRGQRGLIVAPPRTGKTILLQSLANAINANNPEVYLIVLLIDERPEEVTDMERTVKKAEV
ISSTFDEPPQRHVQVCEMVLEKAKRLVERKRDVVILLDSITRLGRAYNAVTPSSGRVLSGGLDANALQRPKRFFGAARNI
EEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEIYLDRHLSEKRVFPAIDINRTGTRKEDLLLPEDVLNRVWILRKIL
APMSAIDSMEFLLDKMRGTKSNKDFMNAMGK
>Mature_431_residues
MRKKKATPTLLTDSALSLTDLKTRSMQELMDLAEQYEIENASSMRKQELIFALLSTCASQNGAIYGDGVLEILPDGFGFL
RSPLCSYMPGPDDIYVSPSQIRRFSLRKGDIVSGQIRPPKEGERYFALLKVTEIGFEPPEHAKNLVLFDNLTPIYPDHQL
VMENGEKNLSNRVIDIMAPIGRGQRGLIVAPPRTGKTILLQSLANAINANNPEVYLIVLLIDERPEEVTDMERTVKKAEV
ISSTFDEPPQRHVQVCEMVLEKAKRLVERKRDVVILLDSITRLGRAYNAVTPSSGRVLSGGLDANALQRPKRFFGAARNI
EEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEIYLDRHLSEKRVFPAIDINRTGTRKEDLLLPEDVLNRVWILRKIL
APMSAIDSMEFLLDKMRGTKSNKDFMNAMGK

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=419, Percent_Identity=65.6324582338902, Blast_Score=575, Evalue=1e-165,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48294; Mature: 48294

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKKATPTLLTDSALSLTDLKTRSMQELMDLAEQYEIENASSMRKQELIFALLSTCASQ
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
NGAIYGDGVLEILPDGFGFLRSPLCSYMPGPDDIYVSPSQIRRFSLRKGDIVSGQIRPPK
CCCEECCCHHEECCCCHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCCCEEECCCCCCC
EGERYFALLKVTEIGFEPPEHAKNLVLFDNLTPIYPDHQLVMENGEKNLSNRVIDIMAPI
CCCCEEEEEEEECCCCCCHHHHCCEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHCC
GRGQRGLIVAPPRTGKTILLQSLANAINANNPEVYLIVLLIDERPEEVTDMERTVKKAEV
CCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHH
ISSTFDEPPQRHVQVCEMVLEKAKRLVERKRDVVILLDSITRLGRAYNAVTPSSGRVLSG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCEEEC
GLDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEIYLDR
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEEEC
HLSEKRVFPAIDINRTGTRKEDLLLPEDVLNRVWILRKILAPMSAIDSMEFLLDKMRGTK
CCCCCCCCCEECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SNKDFMNAMGK
CCHHHHHHCCC
>Mature Secondary Structure
MRKKKATPTLLTDSALSLTDLKTRSMQELMDLAEQYEIENASSMRKQELIFALLSTCASQ
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
NGAIYGDGVLEILPDGFGFLRSPLCSYMPGPDDIYVSPSQIRRFSLRKGDIVSGQIRPPK
CCCEECCCHHEECCCCHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCCCEEECCCCCCC
EGERYFALLKVTEIGFEPPEHAKNLVLFDNLTPIYPDHQLVMENGEKNLSNRVIDIMAPI
CCCCEEEEEEEECCCCCCHHHHCCEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHCC
GRGQRGLIVAPPRTGKTILLQSLANAINANNPEVYLIVLLIDERPEEVTDMERTVKKAEV
CCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHH
ISSTFDEPPQRHVQVCEMVLEKAKRLVERKRDVVILLDSITRLGRAYNAVTPSSGRVLSG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCEEEC
GLDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEIYLDR
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEEEC
HLSEKRVFPAIDINRTGTRKEDLLLPEDVLNRVWILRKILAPMSAIDSMEFLLDKMRGTK
CCCCCCCCCEECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SNKDFMNAMGK
CCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]