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The map label for this gene is carA

Identifier: 220904369

GI number: 220904369

Start: 1288982

End: 1290112

Strand: Direct

Name: carA

Synonym: Ddes_1097

Alternate gene names: 220904369

Gene position: 1288982-1290112 (Clockwise)

Preceding gene: 220904368

Following gene: 220904370

Centisome position: 44.86

GC content: 55.97

Gene sequence:

>1131_bases
ATGAAAGCATTGCTGGTGCTGGAAGACGGGTTCACGCTGGAAGGCAAGTCCTTTACCGGCGATTTTGAAACCGGCGGTGA
AGTCATCTTCACCACAGGGATGACCGGATATCAGGAAATTCTTACGGATCCTTCCTATTACGGGCAAATGGTCTGCATGA
CCTACCCTCTTATAGGCAACTACGGCACTTGCCGTGAAGACATGGAATCCGCCGGAGTGCATTGTGCGGCCCTGCTGGTC
AAGGAATGTTGCAAGAAACCCTCCAACTGGCGTTCCACAATGTCTCTGCCGGAATTCATGAAGCGTTATGAAAAACCCGG
TGTGGAAGGGCTTGATACCCGCGCGCTGACCCGGCATCTGCGCATGAACGGCGCCATGAGAGGCATCATTTCTACACGGG
AAACAGACCCTGCCGTTCTTCAGGAGCGTGCGCGTGCCCTGCCTGCCATGAAAGGGCACAATCTTGTTCCTTTTGTGGCG
CCAGAAAAGCCGTACGCCTGGTATGACAATGCCGTGCAGGAAGTCACCGTGGCTGAAGACGGCAGCTATGCGTGGCGCGG
AACCGGTTTGCCCTTGCTGGTTTATGATTTCGGGATAAAGTGGAACATCTTGCGCCGTCTTTGCGAAGCAGGTTTTGAGC
CACTGGCCGTGCCACCGGGCTTCAGCCCTGCCAGGGCAAAAGCCAGCGGAGCCAAGGGCGTTTTCTTGTCCAACGGCCCT
GGCGATCCGGCGACACTCACCAGCGAAATCGCTCTGGTGCGCGAACTCATACATATGCTGCCGGTTACGGGCATTTGCCT
GGGGCATCAGCTCATCGGGCATGCCCTCGGCGCCCGAACGGAGAAGCTCAAGTTCGGGCATCACGGCTGCAATCATCCGG
TCAAGGACCTGACCACAGGGCGCATTGAAATATCATCACAAAACCACGGATTTCATGTGGTTCTTGATGATGTGGACGAT
GTGGAGGCCACGCATGTCAACCTGAATGACCAGACCCTTGAGGGGCTGCGCCATAAAACACTGCCTGTCATGAGCCTGCA
ATATCATCCGGAGGCCGCTGCCGGACCTCATGATGGCGAGTACCTCTTCAACCGTTTCAGAAAAATCATTGGCGAGAGCG
CCGGAGCCTGA

Upstream 100 bases:

>100_bases
CAGCGGCACGCGCCACGGCTACGGCCATAGGGTCGCGCCGGGACGAGATTCATGTGGAAAGTTTGCAGGAATACTACGCG
CGGGATGCGCGGGGGTAATT

Downstream 100 bases:

>100_bases
TGCCCGGAAAATACTTGATATACAGGTATGCTTGTGGCTAAAGTACATTCACTGTACGAGGTTTTTACTGGCGGCGCATG
CCGCCTCGCCTTAAACTGCA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKALLVLEDGFTLEGKSFTGDFETGGEVIFTTGMTGYQEILTDPSYYGQMVCMTYPLIGNYGTCREDMESAGVHCAALLV
KECCKKPSNWRSTMSLPEFMKRYEKPGVEGLDTRALTRHLRMNGAMRGIISTRETDPAVLQERARALPAMKGHNLVPFVA
PEKPYAWYDNAVQEVTVAEDGSYAWRGTGLPLLVYDFGIKWNILRRLCEAGFEPLAVPPGFSPARAKASGAKGVFLSNGP
GDPATLTSEIALVRELIHMLPVTGICLGHQLIGHALGARTEKLKFGHHGCNHPVKDLTTGRIEISSQNHGFHVVLDDVDD
VEATHVNLNDQTLEGLRHKTLPVMSLQYHPEAAAGPHDGEYLFNRFRKIIGESAGA

Sequences:

>Translated_376_residues
MKALLVLEDGFTLEGKSFTGDFETGGEVIFTTGMTGYQEILTDPSYYGQMVCMTYPLIGNYGTCREDMESAGVHCAALLV
KECCKKPSNWRSTMSLPEFMKRYEKPGVEGLDTRALTRHLRMNGAMRGIISTRETDPAVLQERARALPAMKGHNLVPFVA
PEKPYAWYDNAVQEVTVAEDGSYAWRGTGLPLLVYDFGIKWNILRRLCEAGFEPLAVPPGFSPARAKASGAKGVFLSNGP
GDPATLTSEIALVRELIHMLPVTGICLGHQLIGHALGARTEKLKFGHHGCNHPVKDLTTGRIEISSQNHGFHVVLDDVDD
VEATHVNLNDQTLEGLRHKTLPVMSLQYHPEAAAGPHDGEYLFNRFRKIIGESAGA
>Mature_376_residues
MKALLVLEDGFTLEGKSFTGDFETGGEVIFTTGMTGYQEILTDPSYYGQMVCMTYPLIGNYGTCREDMESAGVHCAALLV
KECCKKPSNWRSTMSLPEFMKRYEKPGVEGLDTRALTRHLRMNGAMRGIISTRETDPAVLQERARALPAMKGHNLVPFVA
PEKPYAWYDNAVQEVTVAEDGSYAWRGTGLPLLVYDFGIKWNILRRLCEAGFEPLAVPPGFSPARAKASGAKGVFLSNGP
GDPATLTSEIALVRELIHMLPVTGICLGHQLIGHALGARTEKLKFGHHGCNHPVKDLTTGRIEISSQNHGFHVVLDDVDD
VEATHVNLNDQTLEGLRHKTLPVMSLQYHPEAAAGPHDGEYLFNRFRKIIGESAGA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=387, Percent_Identity=39.7932816537468, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI169790915, Length=383, Percent_Identity=33.9425587467363, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI21361331, Length=383, Percent_Identity=33.9425587467363, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1786215, Length=384, Percent_Identity=42.7083333333333, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI193204318, Length=382, Percent_Identity=36.9109947643979, Blast_Score=225, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=401, Percent_Identity=37.1571072319202, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6324878, Length=388, Percent_Identity=36.5979381443299, Blast_Score=227, Evalue=2e-60,
Organism=Drosophila melanogaster, GI45555749, Length=390, Percent_Identity=38.2051282051282, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24642586, Length=390, Percent_Identity=38.2051282051282, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_DESDA (B8IZS6)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479681.1
- ProteinModelPortal:   B8IZS6
- GeneID:   7284779
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1097
- HOGENOM:   HBG286341
- ProtClustDB:   PRK12564
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 41241; Mature: 41241

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 266-266 ACT_SITE 349-349 ACT_SITE 351-351

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALLVLEDGFTLEGKSFTGDFETGGEVIFTTGMTGYQEILTDPSYYGQMVCMTYPLIGN
CCEEEEEECCCEECCCCCCCCCCCCCCEEEECCCCHHHHHHCCCHHHCEEEEEEECCCCC
YGTCREDMESAGVHCAALLVKECCKKPSNWRSTMSLPEFMKRYEKPGVEGLDTRALTRHL
CCCCHHHHHHCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHCCCCCCCCHHHHHHHHH
RMNGAMRGIISTRETDPAVLQERARALPAMKGHNLVPFVAPEKPYAWYDNAVQEVTVAED
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHCCHHHEEEEECC
GSYAWRGTGLPLLVYDFGIKWNILRRLCEAGFEPLAVPPGFSPARAKASGAKGVFLSNGP
CCEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCEECCCCCCHHHCCCCCCCEEEEECCC
GDPATLTSEIALVRELIHMLPVTGICLGHQLIGHALGARTEKLKFGHHGCNHPVKDLTTG
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCHHHCCCC
RIEISSQNHGFHVVLDDVDDVEATHVNLNDQTLEGLRHKTLPVMSLQYHPEAAAGPHDGE
EEEEECCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCHH
YLFNRFRKIIGESAGA
HHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKALLVLEDGFTLEGKSFTGDFETGGEVIFTTGMTGYQEILTDPSYYGQMVCMTYPLIGN
CCEEEEEECCCEECCCCCCCCCCCCCCEEEECCCCHHHHHHCCCHHHCEEEEEEECCCCC
YGTCREDMESAGVHCAALLVKECCKKPSNWRSTMSLPEFMKRYEKPGVEGLDTRALTRHL
CCCCHHHHHHCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHCCCCCCCCHHHHHHHHH
RMNGAMRGIISTRETDPAVLQERARALPAMKGHNLVPFVAPEKPYAWYDNAVQEVTVAED
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHCCHHHEEEEECC
GSYAWRGTGLPLLVYDFGIKWNILRRLCEAGFEPLAVPPGFSPARAKASGAKGVFLSNGP
CCEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCEECCCCCCHHHCCCCCCCEEEEECCC
GDPATLTSEIALVRELIHMLPVTGICLGHQLIGHALGARTEKLKFGHHGCNHPVKDLTTG
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCHHHCCCC
RIEISSQNHGFHVVLDDVDDVEATHVNLNDQTLEGLRHKTLPVMSLQYHPEAAAGPHDGE
EEEEECCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCHH
YLFNRFRKIIGESAGA
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA