Click here to switch to the map view.

The map label for this gene is hslU

Identifier: 220904285

GI number: 220904285

Start: 1180342

End: 1181655

Strand: Direct

Name: hslU

Synonym: Ddes_1012

Alternate gene names: 220904285

Gene position: 1180342-1181655 (Clockwise)

Preceding gene: 220904283

Following gene: 220904286

Centisome position: 41.08

GC content: 53.88

Gene sequence:

>1314_bases
ATGAGCACTTTGACACCGCGTGAGATTGTGGCTGAACTCAATAAATTTGTCGTGGGCCAGGAGCAGGCCAAGCGCATGGT
TGCCGTTGCCGTGCGCAATCGCTGGCGCAGGCAGCATCTTCCGTCGGATCTGCGCGATGAAGTTTCGCCGAAAAATATTA
TCATGATGGGTCCCACCGGCGTGGGCAAAACAGAGATCGCCCGCCGTCTGGCCCGGCTTTCCGGTGCACCTTTCATCAAG
GTTGAAGCCACAAAGTTTACCGAAGTGGGCTATGTGGGACGCGATGTGGAATCTATGGTGCGAGATCTTATGGAAATCGG
CATCAACCTCGTACGCGACGAAGAGAACGCCCGTGTGCGCAAGGCTGCCGAAGCCGCTGCGGAATCGCGCCTTATGGATC
TTTTGCTGCCCAACTCCTTTGGTCAGGAGGAGCGGGCATCCACCCGTGAAAAACTTTTGCAGCAGTTTCGTCTGGGCTTT
CTGGATGAGCGCGAAGTGGAAATGGAAGTGACGGAACAGGGCGGAGGTGGCGTGGATATTTTCGCTATCCCCGGCATGGA
GCAGATGGGCGGCCAGGTCAAGGACATGTTCAGCAAGGCCTTTCCCCCCAAGCACAGCCGCCGGAAAATGAAAATTCGCG
ATGCCTTCAATGTGCTGGTACAGGAAGAATCGGGAAAACTGGTGGATCAGGATGCCCTGAGCCAACGCGCCAAAGAGAGG
GTGGAGCAGACAGGCATCATCTTTATTGACGAAATAGACAAGATTGCCAGTTCTTCGCAAAACCGTACATCGGACATTTC
GCGCGAAGGGGTGCAACGTGACCTTTTGCCTATAGTGGAAGGCAGTTCGGTAAATACCAAGTACGGCATGATACGCACCG
ACCACATTCTTTTTATCGCCGCCGGGGCGTTTCACTTCAGCAAACCGTCAGACATGATTCCCGAACTGCAGGGGCGTTTT
CCGCTGCGTGTGGAGCTTCAGGCTCTGGGCCGTGAGGAGTTTTTGCGTATCCTCACCGAGCCTGACAATGCGCTCACCAA
GCAGTACGAAGCCTTGCTCGGCACAGAGCAGATACGCCTGAGCTTTACTATGGACGGGCTGGAAGAGATTGCCGCCTTTG
CTGAAGATATCAACTCACGTACAGAAAATATCGGCGCACGCCGTCTGTATACCATTATGGAAAAAATTCTGGCCGACATT
TCATTTGATGCTCCGGATATGCCGGGCGCGCAGATTGTGGTCAACAAGGATTATGTGGTTGAGCACTTGCAGGATGTACG
CGGCGATCAGGATTTGACGCAGTATATTCTCTAG

Upstream 100 bases:

>100_bases
GTTCTGCCGCGCAGGAGCGTTTTACCCCCTGGCGGCGGGTTTGAAGCCGCCGGGGCAGACCGCTTTTTTCTACCGGCTTG
CCCAAAATCAGGAGCGATTG

Downstream 100 bases:

>100_bases
ATCCAGAGCAATTTCATTTTGAAGTGCTTGCGTAACGGCGTACATCTCTGCTGTCTTCGCCGTTACTTCCTTTGTGGTGA
TGCCCGAGGCACTCCCGGTT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSTLTPREIVAELNKFVVGQEQAKRMVAVAVRNRWRRQHLPSDLRDEVSPKNIIMMGPTGVGKTEIARRLARLSGAPFIK
VEATKFTEVGYVGRDVESMVRDLMEIGINLVRDEENARVRKAAEAAAESRLMDLLLPNSFGQEERASTREKLLQQFRLGF
LDEREVEMEVTEQGGGGVDIFAIPGMEQMGGQVKDMFSKAFPPKHSRRKMKIRDAFNVLVQEESGKLVDQDALSQRAKER
VEQTGIIFIDEIDKIASSSQNRTSDISREGVQRDLLPIVEGSSVNTKYGMIRTDHILFIAAGAFHFSKPSDMIPELQGRF
PLRVELQALGREEFLRILTEPDNALTKQYEALLGTEQIRLSFTMDGLEEIAAFAEDINSRTENIGARRLYTIMEKILADI
SFDAPDMPGAQIVVNKDYVVEHLQDVRGDQDLTQYIL

Sequences:

>Translated_437_residues
MSTLTPREIVAELNKFVVGQEQAKRMVAVAVRNRWRRQHLPSDLRDEVSPKNIIMMGPTGVGKTEIARRLARLSGAPFIK
VEATKFTEVGYVGRDVESMVRDLMEIGINLVRDEENARVRKAAEAAAESRLMDLLLPNSFGQEERASTREKLLQQFRLGF
LDEREVEMEVTEQGGGGVDIFAIPGMEQMGGQVKDMFSKAFPPKHSRRKMKIRDAFNVLVQEESGKLVDQDALSQRAKER
VEQTGIIFIDEIDKIASSSQNRTSDISREGVQRDLLPIVEGSSVNTKYGMIRTDHILFIAAGAFHFSKPSDMIPELQGRF
PLRVELQALGREEFLRILTEPDNALTKQYEALLGTEQIRLSFTMDGLEEIAAFAEDINSRTENIGARRLYTIMEKILADI
SFDAPDMPGAQIVVNKDYVVEHLQDVRGDQDLTQYIL
>Mature_436_residues
STLTPREIVAELNKFVVGQEQAKRMVAVAVRNRWRRQHLPSDLRDEVSPKNIIMMGPTGVGKTEIARRLARLSGAPFIKV
EATKFTEVGYVGRDVESMVRDLMEIGINLVRDEENARVRKAAEAAAESRLMDLLLPNSFGQEERASTREKLLQQFRLGFL
DEREVEMEVTEQGGGGVDIFAIPGMEQMGGQVKDMFSKAFPPKHSRRKMKIRDAFNVLVQEESGKLVDQDALSQRAKERV
EQTGIIFIDEIDKIASSSQNRTSDISREGVQRDLLPIVEGSSVNTKYGMIRTDHILFIAAGAFHFSKPSDMIPELQGRFP
LRVELQALGREEFLRILTEPDNALTKQYEALLGTEQIRLSFTMDGLEEIAAFAEDINSRTENIGARRLYTIMEKILADIS
FDAPDMPGAQIVVNKDYVVEHLQDVRGDQDLTQYIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Homo sapiens, GI7242140, Length=261, Percent_Identity=30.6513409961686, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1790366, Length=444, Percent_Identity=53.1531531531532, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI1786642, Length=233, Percent_Identity=33.0472103004292, Blast_Score=99, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71982908, Length=238, Percent_Identity=31.9327731092437, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982905, Length=238, Percent_Identity=31.9327731092437, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319704, Length=128, Percent_Identity=35.9375, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648291, Length=257, Percent_Identity=33.0739299610895, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648289, Length=257, Percent_Identity=33.0739299610895, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_DESDA (B8IZJ2)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479597.1
- GeneID:   7284692
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1012
- HOGENOM:   HBG745965
- ProtClustDB:   PRK05201
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2

EC number: NA

Molecular weight: Translated: 49272; Mature: 49141

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 250-250 BINDING 315-315 BINDING 387-387

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLTPREIVAELNKFVVGQEQAKRMVAVAVRNRWRRQHLPSDLRDEVSPKNIIMMGPTG
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCC
VGKTEIARRLARLSGAPFIKVEATKFTEVGYVGRDVESMVRDLMEIGINLVRDEENARVR
CCHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHCCEEEECCHHHHHH
KAAEAAAESRLMDLLLPNSFGQEERASTREKLLQQFRLGFLDEREVEMEVTEQGGGGVDI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEE
FAIPGMEQMGGQVKDMFSKAFPPKHSRRKMKIRDAFNVLVQEESGKLVDQDALSQRAKER
EECCCHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VEQTGIIFIDEIDKIASSSQNRTSDISREGVQRDLLPIVEGSSVNTKYGMIRTDHILFIA
HHHCCEEEEECHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEE
AGAFHFSKPSDMIPELQGRFPLRVELQALGREEFLRILTEPDNALTKQYEALLGTEQIRL
ECCCCCCCCHHHHHHHCCCCCEEEEHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEE
SFTMDGLEEIAAFAEDINSRTENIGARRLYTIMEKILADISFDAPDMPGAQIVVNKDYVV
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHH
EHLQDVRGDQDLTQYIL
HHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
STLTPREIVAELNKFVVGQEQAKRMVAVAVRNRWRRQHLPSDLRDEVSPKNIIMMGPTG
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCC
VGKTEIARRLARLSGAPFIKVEATKFTEVGYVGRDVESMVRDLMEIGINLVRDEENARVR
CCHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHCCEEEECCHHHHHH
KAAEAAAESRLMDLLLPNSFGQEERASTREKLLQQFRLGFLDEREVEMEVTEQGGGGVDI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEE
FAIPGMEQMGGQVKDMFSKAFPPKHSRRKMKIRDAFNVLVQEESGKLVDQDALSQRAKER
EECCCHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VEQTGIIFIDEIDKIASSSQNRTSDISREGVQRDLLPIVEGSSVNTKYGMIRTDHILFIA
HHHCCEEEEECHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEE
AGAFHFSKPSDMIPELQGRFPLRVELQALGREEFLRILTEPDNALTKQYEALLGTEQIRL
ECCCCCCCCHHHHHHHCCCCCEEEEHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEE
SFTMDGLEEIAAFAEDINSRTENIGARRLYTIMEKILADISFDAPDMPGAQIVVNKDYVV
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHH
EHLQDVRGDQDLTQYIL
HHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA