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The map label for this gene is hemA

Identifier: 220904232

GI number: 220904232

Start: 1118404

End: 1119696

Strand: Reverse

Name: hemA

Synonym: Ddes_0959

Alternate gene names: 220904232

Gene position: 1119696-1118404 (Counterclockwise)

Preceding gene: 220904233

Following gene: 220904231

Centisome position: 38.97

GC content: 56.23

Gene sequence:

>1293_bases
ATGGACTGTGATATCTTTCTTGTGGGCCTGAATCACCGCACCGCCGGAGTGGATGTGCGCGAACGCTTCGCCCTGGCCAA
TCATTGCGATGAGGAACACTGGGCTTTGCCATGTACCGGCGCGGTGAGCGAGAGTATTATACTCTCTACCTGTAACCGCG
TGGAAATCCTGGCCGCCGGAACCGGCGAAGTGGCGGAACAGGTACTGCGCAACTGGGCCGGAGCGCGAAAAAGTGACGTT
GAGGATCTTAGGCCCTACGTCTATGTGCATAAAAATCTTGAGGCGGTACGGCACCTGTTTTCCGTGGCATCAAGCCTGGA
TTCAATGGTGCTGGGCGAACCCCAGATTCTCGGGCAGTTGAAAACAGCCTACCGCAAGGCTGTCAAAAGCCGCGCCACTG
GGGTCATCCTCAACCGTCTGCTGCACAAAGCTTTTTCGGTGGCAAAAAGAGTACGTACTGAAACCGCCGTGGCTTCCAGC
GCGGTTTCCATCAGCTATGCCGCCGTGGAACTGGCAAAACGCATTTTCGGCGACATGCGCGCCCACAAGGCCATGCTTGT
GGGCGCGGGCGAAATGGCCGAACTGGCCGCCATGCATCTGCTCCAGGCCGGGATTGCCGACATACTTGTAGCCAACCGCA
CCCTTGTGCGCGGGCAGGAACTGGCAAAACAGTTCAACGGGCACGCCATACCTTTTGAAGATATGCCCCGCCACCTGCTG
GATGTGGACATCATCATCACTTCCACCGGTTCGCAGGAACCAATTATCCGCGCCCGCGACATCCGCGCCGCCCTCAAGAT
CCGTAAGAACAGGCCCATGTTTTTTATTGATATTGCTGTTCCCCGTGACATTGATCCCGACGTTAACGGTCTGGACAACG
TGTACCTTTATGACATTGACGACCTCAAGGAGGTTGTTGAAGAAAACCTCGCCACCCGCCGTGATGAAGCGGCCAAGGCG
GCGGAAATCGTCAATGAGGAAGTGGTGCAGTTCTCCCGCTGGCTTGCCAGCCTGGACATGCAGCCCACCATTGTGGACCT
TATCAAAAAAGGCCAGCGCGCCGCGGAAGAAGAACTGGCAAAAACCCTCAAGCGGCTCGGCCCTGTGGACGACAATACGC
GTGAGGCTCTTGAAGCCATGGCGGGCGCACTGGTACGCAAGCTCAACCACGACCCCATCATGTTTCTGAAACACGGCGGC
ATGTCGCAAGAAGGCAACGGGCCGCGTATAAGCATAATGCGCCGTATTTTCAATCTGGATAAAACAGGCTGTATTTACTC
GGAGGAAAACTGA

Upstream 100 bases:

>100_bases
CTGTTTTTATTTTTATTTTGTGTCTTTTTTCAATCATTGTGGTGAATACCTTTATGGAGACGCACCACGCATTCATCCGG
CGCTGACTTGGGCATGAACT

Downstream 100 bases:

>100_bases
TGCGCTGGTACGGCATCGACGACCTCACACCCGAAGACACGGCAAAAATAGCCGACAGACTCAAAGAAATGGGGCTTGAG
GCCGGTCTTGAGGGTCTGTA

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MDCDIFLVGLNHRTAGVDVRERFALANHCDEEHWALPCTGAVSESIILSTCNRVEILAAGTGEVAEQVLRNWAGARKSDV
EDLRPYVYVHKNLEAVRHLFSVASSLDSMVLGEPQILGQLKTAYRKAVKSRATGVILNRLLHKAFSVAKRVRTETAVASS
AVSISYAAVELAKRIFGDMRAHKAMLVGAGEMAELAAMHLLQAGIADILVANRTLVRGQELAKQFNGHAIPFEDMPRHLL
DVDIIITSTGSQEPIIRARDIRAALKIRKNRPMFFIDIAVPRDIDPDVNGLDNVYLYDIDDLKEVVEENLATRRDEAAKA
AEIVNEEVVQFSRWLASLDMQPTIVDLIKKGQRAAEEELAKTLKRLGPVDDNTREALEAMAGALVRKLNHDPIMFLKHGG
MSQEGNGPRISIMRRIFNLDKTGCIYSEEN

Sequences:

>Translated_430_residues
MDCDIFLVGLNHRTAGVDVRERFALANHCDEEHWALPCTGAVSESIILSTCNRVEILAAGTGEVAEQVLRNWAGARKSDV
EDLRPYVYVHKNLEAVRHLFSVASSLDSMVLGEPQILGQLKTAYRKAVKSRATGVILNRLLHKAFSVAKRVRTETAVASS
AVSISYAAVELAKRIFGDMRAHKAMLVGAGEMAELAAMHLLQAGIADILVANRTLVRGQELAKQFNGHAIPFEDMPRHLL
DVDIIITSTGSQEPIIRARDIRAALKIRKNRPMFFIDIAVPRDIDPDVNGLDNVYLYDIDDLKEVVEENLATRRDEAAKA
AEIVNEEVVQFSRWLASLDMQPTIVDLIKKGQRAAEEELAKTLKRLGPVDDNTREALEAMAGALVRKLNHDPIMFLKHGG
MSQEGNGPRISIMRRIFNLDKTGCIYSEEN
>Mature_430_residues
MDCDIFLVGLNHRTAGVDVRERFALANHCDEEHWALPCTGAVSESIILSTCNRVEILAAGTGEVAEQVLRNWAGARKSDV
EDLRPYVYVHKNLEAVRHLFSVASSLDSMVLGEPQILGQLKTAYRKAVKSRATGVILNRLLHKAFSVAKRVRTETAVASS
AVSISYAAVELAKRIFGDMRAHKAMLVGAGEMAELAAMHLLQAGIADILVANRTLVRGQELAKQFNGHAIPFEDMPRHLL
DVDIIITSTGSQEPIIRARDIRAALKIRKNRPMFFIDIAVPRDIDPDVNGLDNVYLYDIDDLKEVVEENLATRRDEAAKA
AEIVNEEVVQFSRWLASLDMQPTIVDLIKKGQRAAEEELAKTLKRLGPVDDNTREALEAMAGALVRKLNHDPIMFLKHGG
MSQEGNGPRISIMRRIFNLDKTGCIYSEEN

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=419, Percent_Identity=38.6634844868735, Blast_Score=264, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_DESDA (B8IZD9)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479544.1
- ProteinModelPortal:   B8IZD9
- GeneID:   7284639
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0959
- HOGENOM:   HBG732626
- ProtClustDB:   PRK00045
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 47662; Mature: 47662

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 51-51 BINDING 108-108 BINDING 119-119

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCDIFLVGLNHRTAGVDVRERFALANHCDEEHWALPCTGAVSESIILSTCNRVEILAAG
CCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECC
TGEVAEQVLRNWAGARKSDVEDLRPYVYVHKNLEAVRHLFSVASSLDSMVLGEPQILGQL
CHHHHHHHHHHHCCCCCCCHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KTAYRKAVKSRATGVILNRLLHKAFSVAKRVRTETAVASSAVSISYAAVELAKRIFGDMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHKAMLVGAGEMAELAAMHLLQAGIADILVANRTLVRGQELAKQFNGHAIPFEDMPRHLL
HHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHE
DVDIIITSTGSQEPIIRARDIRAALKIRKNRPMFFIDIAVPRDIDPDVNGLDNVYLYDID
EEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEEHH
DLKEVVEENLATRRDEAAKAAEIVNEEVVQFSRWLASLDMQPTIVDLIKKGQRAAEEELA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KTLKRLGPVDDNTREALEAMAGALVRKLNHDPIMFLKHGGMSQEGNGPRISIMRRIFNLD
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCC
KTGCIYSEEN
CCCCEECCCC
>Mature Secondary Structure
MDCDIFLVGLNHRTAGVDVRERFALANHCDEEHWALPCTGAVSESIILSTCNRVEILAAG
CCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECC
TGEVAEQVLRNWAGARKSDVEDLRPYVYVHKNLEAVRHLFSVASSLDSMVLGEPQILGQL
CHHHHHHHHHHHCCCCCCCHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KTAYRKAVKSRATGVILNRLLHKAFSVAKRVRTETAVASSAVSISYAAVELAKRIFGDMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHKAMLVGAGEMAELAAMHLLQAGIADILVANRTLVRGQELAKQFNGHAIPFEDMPRHLL
HHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHE
DVDIIITSTGSQEPIIRARDIRAALKIRKNRPMFFIDIAVPRDIDPDVNGLDNVYLYDID
EEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEEHH
DLKEVVEENLATRRDEAAKAAEIVNEEVVQFSRWLASLDMQPTIVDLIKKGQRAAEEELA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KTLKRLGPVDDNTREALEAMAGALVRKLNHDPIMFLKHGGMSQEGNGPRISIMRRIFNLD
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCC
KTGCIYSEEN
CCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA