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The map label for this gene is ispA [C]

Identifier: 220904228

GI number: 220904228

Start: 1112280

End: 1113176

Strand: Direct

Name: ispA [C]

Synonym: Ddes_0955

Alternate gene names: 220904228

Gene position: 1112280-1113176 (Clockwise)

Preceding gene: 220904227

Following gene: 220904229

Centisome position: 38.71

GC content: 61.98

Gene sequence:

>897_bases
GTGATGATGCCAGTATCCCGGATGAAGGAACTTTTGCGCGAACGCGGGCAAATGGTGGAAGAAGAGCTTGCCGTCTGCCT
TGACGGCCGTGCTGTGCCGCAGCGCCTCAAGGACTCTATGCTATACAGCCTCCAGGCTGGCGGTAAACGCCTGCGCCCTG
TACTCTGCCTGAGCGCTGCCGCCCTGTGCGGTCTGCCGCTGCGCGATGCTTTGCCCTTTGCCTCGGCCATCGAGATGATT
CATACCTATTCGCTGGTACATGATGATCTTCCGGCTATGGATGATGACGACCTGCGCCGTGGCAAGCCTTCAAACCACAA
AGCATTTGACGAGGCCACAGCCATTCTTGCGGGTGATGGTCTGCTGACGGACGCATTTCTGATGATGTGCCGCAGCCCCC
TGGCCCCGCCGCGCGTGTTGCGGGCCATCAGCGAACTGGCCCTGGCCGCCGGCTCTTCCGGCATGGTGGGCGGGCAGGAA
TGGGACATGATATTTACCGGCAAGGACTTGGTATCCATTGAAGACCTGCGCGGCATTCACGCCATGAAGACAGGCGCGCT
GCTGCGGGCTTCCTGTGTATGCGGGGCCATGCTGGCCGGGGCTTCGGATGAAGACCTGGCTGCCATCGGCTCCTATGGCG
CAGCCCTTGGCGTGGCGTTTCAGATTGCCGATGACATCCTTGACGTGGTGGCTGACACCGCCACTCTGGGCAAACCCGTG
GGCAGCGATGCAGCCCAGGGCAAGAAGACCTATCCCGCCCTGCTGGGGCTTGACCAGAGCCGTGCTCTGGCGCGGGAGCA
GGCCGAAAAGGCCAAGGCCGCACTTGCGGGCTTCAGCGGGGACGAGGCGGATTTTTTGCGTGCATTAGCTGACTATACGG
TCACCAGGGCGGCCTGA

Upstream 100 bases:

>100_bases
GGATGGTCAGGCAAACCCCATGAGTTCCCAGGGCCATACGGCCGGGGAATATCCCGATGATGAAGCGGAAGAAGCCGAGG
AAGCGTAGGAGGCTTGACAG

Downstream 100 bases:

>100_bases
CATGCCTGACACACATATAATGACGGCGCAGGATGTTTCTGTGGGAGCCGGAGCGGCACAGGCTGCCGATATGCCGGAAC
CTGCCGCGCGGGATGAAAAC

Product: polyprenyl synthetase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MMMPVSRMKELLRERGQMVEEELAVCLDGRAVPQRLKDSMLYSLQAGGKRLRPVLCLSAAALCGLPLRDALPFASAIEMI
HTYSLVHDDLPAMDDDDLRRGKPSNHKAFDEATAILAGDGLLTDAFLMMCRSPLAPPRVLRAISELALAAGSSGMVGGQE
WDMIFTGKDLVSIEDLRGIHAMKTGALLRASCVCGAMLAGASDEDLAAIGSYGAALGVAFQIADDILDVVADTATLGKPV
GSDAAQGKKTYPALLGLDQSRALAREQAEKAKAALAGFSGDEADFLRALADYTVTRAA

Sequences:

>Translated_298_residues
MMMPVSRMKELLRERGQMVEEELAVCLDGRAVPQRLKDSMLYSLQAGGKRLRPVLCLSAAALCGLPLRDALPFASAIEMI
HTYSLVHDDLPAMDDDDLRRGKPSNHKAFDEATAILAGDGLLTDAFLMMCRSPLAPPRVLRAISELALAAGSSGMVGGQE
WDMIFTGKDLVSIEDLRGIHAMKTGALLRASCVCGAMLAGASDEDLAAIGSYGAALGVAFQIADDILDVVADTATLGKPV
GSDAAQGKKTYPALLGLDQSRALAREQAEKAKAALAGFSGDEADFLRALADYTVTRAA
>Mature_298_residues
MMMPVSRMKELLRERGQMVEEELAVCLDGRAVPQRLKDSMLYSLQAGGKRLRPVLCLSAAALCGLPLRDALPFASAIEMI
HTYSLVHDDLPAMDDDDLRRGKPSNHKAFDEATAILAGDGLLTDAFLMMCRSPLAPPRVLRAISELALAAGSSGMVGGQE
WDMIFTGKDLVSIEDLRGIHAMKTGALLRASCVCGAMLAGASDEDLAAIGSYGAALGVAFQIADDILDVVADTATLGKPV
GSDAAQGKKTYPALLGLDQSRALAREQAEKAKAALAGFSGDEADFLRALADYTVTRAA

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=239, Percent_Identity=30.1255230125523, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1786623, Length=267, Percent_Identity=47.565543071161, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789578, Length=239, Percent_Identity=36.4016736401674, Blast_Score=128, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17505681, Length=252, Percent_Identity=30.1587301587302, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6319475, Length=235, Percent_Identity=31.9148936170213, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24651612, Length=233, Percent_Identity=30.0429184549356, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 31468; Mature: 31468

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMPVSRMKELLRERGQMVEEELAVCLDGRAVPQRLKDSMLYSLQAGGKRLRPVLCLSAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALCGLPLRDALPFASAIEMIHTYSLVHDDLPAMDDDDLRRGKPSNHKAFDEATAILAGDG
HHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCC
LLTDAFLMMCRSPLAPPRVLRAISELALAAGSSGMVGGQEWDMIFTGKDLVSIEDLRGIH
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
AMKTGALLRASCVCGAMLAGASDEDLAAIGSYGAALGVAFQIADDILDVVADTATLGKPV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GSDAAQGKKTYPALLGLDQSRALAREQAEKAKAALAGFSGDEADFLRALADYTVTRAA
CCHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMMPVSRMKELLRERGQMVEEELAVCLDGRAVPQRLKDSMLYSLQAGGKRLRPVLCLSAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALCGLPLRDALPFASAIEMIHTYSLVHDDLPAMDDDDLRRGKPSNHKAFDEATAILAGDG
HHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCC
LLTDAFLMMCRSPLAPPRVLRAISELALAAGSSGMVGGQEWDMIFTGKDLVSIEDLRGIH
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
AMKTGALLRASCVCGAMLAGASDEDLAAIGSYGAALGVAFQIADDILDVVADTATLGKPV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GSDAAQGKKTYPALLGLDQSRALAREQAEKAKAALAGFSGDEADFLRALADYTVTRAA
CCHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486607; 7918490 [H]