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The map label for this gene is hyfR [H]

Identifier: 220904191

GI number: 220904191

Start: 1069779

End: 1071443

Strand: Direct

Name: hyfR [H]

Synonym: Ddes_0918

Alternate gene names: 220904191

Gene position: 1069779-1071443 (Clockwise)

Preceding gene: 220904190

Following gene: 220904195

Centisome position: 37.23

GC content: 54.83

Gene sequence:

>1665_bases
ATGATTATTCTTGGAAACAGAAACATAAAGACACTCCTTCTGGAACTTGCGCAGCAGCAGGCTGTGGAAGACGTGCTTTC
TCTGGCGGTCAAAAGTCTGGGAACGGCGCGCGATTCGGCTCTGGTGCGCATATGGCTTGTGGAAGCTGATGACGGTTGCC
CCTCCTGTGCAGACAAGGGCGTCTGCGCAGGGCGTGGCCGCTGTCTGCACCTCAAGGCAAGTTATGGGCAGTCCCGCGTT
GACGGGCGGCTCTGGACGCAGACGGAGCCGTCAGGTTTTTACCGTTTTCCTCTGGGGCACAGAAAGGTCGGGGCCATAGC
CATGACGAACAGGCCCCTTGAGGTTTCGCATATTGAAGGGAATGAAGACTGGATAGCCGCCCCGGAGTGGATTGCGGCAG
AAGGCATTGTGAGCTTTGCCGGGCAGCCCTTGGTGTGCCGTGGTGAAACCCTTGGCGTTATCGCATTTTTTTCGCGTGAT
ACCTTGGCGCAAGGCGCTATGGAAATGTTGCGCATGGTGGCTGACCACATTGCGTATGCCATCACCAATGCCCGGCTTTT
TGAAATAACCCGGTCCATAACGCAGCGAAGAGAACTGGAAAACGCCCACCTGCGCGAAGAACTTTACGAAGCGCGGCAGT
TCACAGGCATCATAGGCAAAAGCGCGGCCATCAAGGCCATACGGGAACAGATACTTGTTGTCAGTGAAACTGAAGCAACA
GTGCTTATTCAGGGGGCTTCCGGCACGGGAAAGGAACTGGTGGCGCATGAGCTGCACAAGAACAGCAAGAGGAAAAATAA
TCCCTTTATAAAAATAAACTGCGCCGCCATACCGCACCATCTGTTTGAGAGCGAATTTTTCGGCCATGTGAAAGGGGCCT
TTACCGGAGCCATCCACGACCGCATGGGTTTTTTCCAGCTTGCGGACGGCGGTACGTTGTTTCTTGACGAAATTGCAGAA
ATCCCTTTGGAACTTCAAGGCAAGCTCCTGCGCGTACTGCAAGAGGGCGAATTCAGGCGGGTGGGGGAAGAGAAAAACCG
CCATACCGACATTCGCCTTATCGCAGCCACCAATAAAGACCTCAAGGCCGCCATACGAAAACGCACATTTCGCGATGACC
TGTACTACCGCCTGCAGGTTTTTCCGCTCACAGTGCCAACTCTCAAGGAGAGGGAGGAAGATATTCCGCTTCTGGTACGC
CATTTTATACGTATTTTCTGCCGTAAAAACGGACGCCGCGTTTTTGATGTGCCGGAAGCGCAGATGAACAGATTGCGCCA
GTACGACTGGCCGGGCAATATCCGTGAATTGCAGAATTTTGTGGAGCGTATGGTCATTACCGGGCGGCCGGAGCAGGCTC
TGGCATATCTTGCCCTGTGTGAAGCGGCGTCGCCGGAAGCGGAAGAAGCGGCTCCGGAGCTGTGTGCTTCTTTTGGCATT
CCCAAAATCCTGACAGAAAAGCAGATGCGCCAGCTGGAAAAAGATAACATGAAGGCGGCGCTGGAGCGGACCAACTGGCT
CATTTACGGAAACCGCGGGGCAGCGGCCTTGCTGGGGATGAAGCCGACCACACTTATTTCGCGGCTCAAGCGGTTTGATC
TCTACAGGCTGCGCCCGAATTCCATTTCTGCTGATGGACCTGCAGGCCCCGCAGGCGCAGACTGA

Upstream 100 bases:

>100_bases
CACTGGAAGTCCGGCGGCAAGCCAGCTACTATGACAGAGGTGACCGCACGCCCGCTTTTTTCATTACTGCCAACTCCGGA
TTACTGCCAATAGCCCAGCC

Downstream 100 bases:

>100_bases
CGCAAAAGGCGAAGTTCCGGCGGCAATGGCGTGTTCCGGAACTTCGCTTTCATTCATCTGTCTGCAGTGCAGACTTGCCT
GGTCAGGCGGCGGGGCAGGA

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MIILGNRNIKTLLLELAQQQAVEDVLSLAVKSLGTARDSALVRIWLVEADDGCPSCADKGVCAGRGRCLHLKASYGQSRV
DGRLWTQTEPSGFYRFPLGHRKVGAIAMTNRPLEVSHIEGNEDWIAAPEWIAAEGIVSFAGQPLVCRGETLGVIAFFSRD
TLAQGAMEMLRMVADHIAYAITNARLFEITRSITQRRELENAHLREELYEARQFTGIIGKSAAIKAIREQILVVSETEAT
VLIQGASGTGKELVAHELHKNSKRKNNPFIKINCAAIPHHLFESEFFGHVKGAFTGAIHDRMGFFQLADGGTLFLDEIAE
IPLELQGKLLRVLQEGEFRRVGEEKNRHTDIRLIAATNKDLKAAIRKRTFRDDLYYRLQVFPLTVPTLKEREEDIPLLVR
HFIRIFCRKNGRRVFDVPEAQMNRLRQYDWPGNIRELQNFVERMVITGRPEQALAYLALCEAASPEAEEAAPELCASFGI
PKILTEKQMRQLEKDNMKAALERTNWLIYGNRGAAALLGMKPTTLISRLKRFDLYRLRPNSISADGPAGPAGAD

Sequences:

>Translated_554_residues
MIILGNRNIKTLLLELAQQQAVEDVLSLAVKSLGTARDSALVRIWLVEADDGCPSCADKGVCAGRGRCLHLKASYGQSRV
DGRLWTQTEPSGFYRFPLGHRKVGAIAMTNRPLEVSHIEGNEDWIAAPEWIAAEGIVSFAGQPLVCRGETLGVIAFFSRD
TLAQGAMEMLRMVADHIAYAITNARLFEITRSITQRRELENAHLREELYEARQFTGIIGKSAAIKAIREQILVVSETEAT
VLIQGASGTGKELVAHELHKNSKRKNNPFIKINCAAIPHHLFESEFFGHVKGAFTGAIHDRMGFFQLADGGTLFLDEIAE
IPLELQGKLLRVLQEGEFRRVGEEKNRHTDIRLIAATNKDLKAAIRKRTFRDDLYYRLQVFPLTVPTLKEREEDIPLLVR
HFIRIFCRKNGRRVFDVPEAQMNRLRQYDWPGNIRELQNFVERMVITGRPEQALAYLALCEAASPEAEEAAPELCASFGI
PKILTEKQMRQLEKDNMKAALERTNWLIYGNRGAAALLGMKPTTLISRLKRFDLYRLRPNSISADGPAGPAGAD
>Mature_554_residues
MIILGNRNIKTLLLELAQQQAVEDVLSLAVKSLGTARDSALVRIWLVEADDGCPSCADKGVCAGRGRCLHLKASYGQSRV
DGRLWTQTEPSGFYRFPLGHRKVGAIAMTNRPLEVSHIEGNEDWIAAPEWIAAEGIVSFAGQPLVCRGETLGVIAFFSRD
TLAQGAMEMLRMVADHIAYAITNARLFEITRSITQRRELENAHLREELYEARQFTGIIGKSAAIKAIREQILVVSETEAT
VLIQGASGTGKELVAHELHKNSKRKNNPFIKINCAAIPHHLFESEFFGHVKGAFTGAIHDRMGFFQLADGGTLFLDEIAE
IPLELQGKLLRVLQEGEFRRVGEEKNRHTDIRLIAATNKDLKAAIRKRTFRDDLYYRLQVFPLTVPTLKEREEDIPLLVR
HFIRIFCRKNGRRVFDVPEAQMNRLRQYDWPGNIRELQNFVERMVITGRPEQALAYLALCEAASPEAEEAAPELCASFGI
PKILTEKQMRQLEKDNMKAALERTNWLIYGNRGAAALLGMKPTTLISRLKRFDLYRLRPNSISADGPAGPAGAD

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=404, Percent_Identity=41.0891089108911, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1789087, Length=393, Percent_Identity=42.2391857506361, Blast_Score=297, Evalue=9e-82,
Organism=Escherichia coli, GI1788550, Length=244, Percent_Identity=50.8196721311475, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1790437, Length=326, Percent_Identity=46.319018404908, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI87082152, Length=303, Percent_Identity=42.2442244224422, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI1788905, Length=247, Percent_Identity=46.5587044534413, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1790299, Length=339, Percent_Identity=40.4129793510324, Blast_Score=222, Evalue=6e-59,
Organism=Escherichia coli, GI1789233, Length=329, Percent_Identity=38.6018237082067, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=40, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1786524, Length=340, Percent_Identity=37.0588235294118, Blast_Score=195, Evalue=7e-51,
Organism=Escherichia coli, GI1787583, Length=257, Percent_Identity=38.5214007782101, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789828, Length=239, Percent_Identity=35.1464435146443, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI87081858, Length=300, Percent_Identity=29.6666666666667, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 62076; Mature: 62076

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILGNRNIKTLLLELAQQQAVEDVLSLAVKSLGTARDSALVRIWLVEADDGCPSCADKG
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCC
VCAGRGRCLHLKASYGQSRVDGRLWTQTEPSGFYRFPLGHRKVGAIAMTNRPLEVSHIEG
CCCCCCEEEEEEECCCCCCCCCEEEECCCCCCEEECCCCCCCCCEEEECCCCEEEEEECC
NEDWIAAPEWIAAEGIVSFAGQPLVCRGETLGVIAFFSRDTLAQGAMEMLRMVADHIAYA
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHH
ITNARLFEITRSITQRRELENAHLREELYEARQFTGIIGKSAAIKAIREQILVVSETEAT
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEECCEE
VLIQGASGTGKELVAHELHKNSKRKNNPFIKINCAAIPHHLFESEFFGHVKGAFTGAIHD
EEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEHHHCHHHHHHHHHHHHHHHHHHHHHH
RMGFFQLADGGTLFLDEIAEIPLELQGKLLRVLQEGEFRRVGEEKNRHTDIRLIAATNKD
CCCEEEECCCCEEEHHHHHHCCHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEECCHH
LKAAIRKRTFRDDLYYRLQVFPLTVPTLKEREEDIPLLVRHFIRIFCRKNGRRVFDVPEA
HHHHHHHHHHHHCCEEEEEEEEECCCCHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCHH
QMNRLRQYDWPGNIRELQNFVERMVITGRPEQALAYLALCEAASPEAEEAAPELCASFGI
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
PKILTEKQMRQLEKDNMKAALERTNWLIYGNRGAAALLGMKPTTLISRLKRFDLYRLRPN
CHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCHHHHHHHHHHCCEEEECCC
SISADGPAGPAGAD
CCCCCCCCCCCCCC
>Mature Secondary Structure
MIILGNRNIKTLLLELAQQQAVEDVLSLAVKSLGTARDSALVRIWLVEADDGCPSCADKG
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCC
VCAGRGRCLHLKASYGQSRVDGRLWTQTEPSGFYRFPLGHRKVGAIAMTNRPLEVSHIEG
CCCCCCEEEEEEECCCCCCCCCEEEECCCCCCEEECCCCCCCCCEEEECCCCEEEEEECC
NEDWIAAPEWIAAEGIVSFAGQPLVCRGETLGVIAFFSRDTLAQGAMEMLRMVADHIAYA
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHH
ITNARLFEITRSITQRRELENAHLREELYEARQFTGIIGKSAAIKAIREQILVVSETEAT
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEECCEE
VLIQGASGTGKELVAHELHKNSKRKNNPFIKINCAAIPHHLFESEFFGHVKGAFTGAIHD
EEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEHHHCHHHHHHHHHHHHHHHHHHHHHH
RMGFFQLADGGTLFLDEIAEIPLELQGKLLRVLQEGEFRRVGEEKNRHTDIRLIAATNKD
CCCEEEECCCCEEEHHHHHHCCHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEECCHH
LKAAIRKRTFRDDLYYRLQVFPLTVPTLKEREEDIPLLVRHFIRIFCRKNGRRVFDVPEA
HHHHHHHHHHHHCCEEEEEEEEECCCCHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCHH
QMNRLRQYDWPGNIRELQNFVERMVITGRPEQALAYLALCEAASPEAEEAAPELCASFGI
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
PKILTEKQMRQLEKDNMKAALERTNWLIYGNRGAAALLGMKPTTLISRLKRFDLYRLRPN
CHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCHHHHHHHHHHCCEEEECCC
SISADGPAGPAGAD
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]