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The map label for this gene is glyS [H]

Identifier: 220904189

GI number: 220904189

Start: 1066422

End: 1068506

Strand: Direct

Name: glyS [H]

Synonym: Ddes_0916

Alternate gene names: 220904189

Gene position: 1066422-1068506 (Clockwise)

Preceding gene: 220904188

Following gene: 220904190

Centisome position: 37.11

GC content: 61.25

Gene sequence:

>2085_bases
GTGGCCACCTTTGTGCTCGAAATCGGCAGTGAAGAACTGCCTTCCCGTTTTTTGGCTCCCGAAGAAGCGGAACTTTTCAC
CCGTTTTACCACGGCTCTTGATGAGGCAGGCCTTGAGTACGGCAGCCTGCGCGTCATGAGTACGCCGCGCCGTGCCGTGG
TTATGGTTGAAAACCTCAATCCCGTGCAGATTGAGCGGGAAGAGGTGATCTCCGGTCCGCCCGTGCGCGTGGCCTATGAC
GCCGAGGGCAAACCCACCAAGGCTCTGGAGGGCTTTGTCCGTACCAATGGCTGCGCCCTGGAAGACGTTTTTCAGCAGGA
AACAGAAAAAGGCGCGTACGTGGCCGTGCGTAAACGCACCGGCGGCGCGCCCGCCAGTGAACTGCTGGCTGTCATGTGCC
CGGCCATCATTTCGGCATTGCCCTTTGCCAAGCGCATGCGCTGGGGCAACCATTCGCTGGCCTACGCCCGCCCCTTGCGC
TGGATTCTGGCCCTGCTGGACGATGAGATTATTTCTTTTGAGGTCGGCCCGATGGTTTCGGGACGCGAAACCTGCGGTCA
CCGCATTCACGGGGCAGGTCCCTTTTCTGTTGCCCGCGCTGCAGAGCTTCCGGCCATACTGGCAGATTCCTGCGGCATCA
CCCTTGATTCCCCGAAGCGCCGGGAAATTATTGTGGAGGGCGGCAACAGGCAGGCGCAGACCGTCGGCGGCAAGGTGCTC
TGGAAGGACAGCCTGCTTGACGAAGTGCAGGGCCTTGCGGAACATCCCGTGCCACTGCTGGCGGATTTTGATCCGGCTTA
TCTTGAAGTGCCGCGCGAGGTGCTGCTGACCAGTATGGAAAGCCACCAGAAAAGTTTCGGCATCGAAGGGGCCGATGGCC
GTTTGCTGCCGCACTTTCTGACGGTGCTCAACATCACTCCCGAAGACATGAGTATTGTCAAGCGCGGGTGGGAAAGAGTG
CTGCGCGCCCGTCTGGAAGACGCCCGCTTTTTCTGGCAGGCCGACCTGCGGGAAGGGTTTGACCACTGGCTGCAAAAACT
CGATTCGGTTATTTTTATCGGTGGGCTTGGCAGTATGGGAGACAAGACACGCCGTCTCGAATCCCTGTGCCGCTGGCTGG
CGGAAAAATGCGCCCCCGACCTTGCCGCTGATGCCGCGCGGGCAGGTCGCCTTTCAAAGGCCGACCTCGTCAGCGGCCTC
GTTGGCGAATTTGACACCCTGCAAGGTGTCATGGGGGGCATTTATGCCGCCCGCAAGGGCGAAAGTGCGGCCGTGGCCCA
GGCTGTGGCCGAGCAGTATCTGCCTGCAGGGCCGGATTCGCCCCTGCCGGGCAGCATGTGCGGCGCGCTGCTGTCACTGG
CCGACAAGGCCGACACGCTCGCGGGCTGTTTCGGCCTGGGGATGATACCCACCGGCGCGGCGGACCCCAACGGTCTTCGC
CGTTGCGCTCTGGGCATCATTCGTATTGTGCTTGATTTTGGCCTGAGGCTCGACATGCGTGAGGTTTTTGCCAGGGCGCA
GGAGCTTTACGGGGACAGGCAATGGAAACTGCCTCCGGCAGAAGCTCTCGATAAACTTATGGAATTCTTTGGCGCAAGGC
TGCGCAACTATTTCATGAGTCAGGGGCAGGACACCTTGCTGGTGGATGCCGCGCTGGGTGCGGGTGCGGCTCAGGTAAAG
GACTGTGGCGAACGCCTGGAAGCTCTGGTGGACTTTAGCCGCGAGGCTGGTTTTGCCGAAGCTGCCCAGACCTTCAAACG
CGTGGCAAATATTTTGCGCAAGCAGGGGCAGGCGGAAGATATCCCGGCCCAGTGGCAGGCGGAGCTTCTGCATGAAGATG
CGGAAAAGGCCCTGGCTGCAACTCTGGATGGCCTGCTGCCGGAACTGGACCGTATGTGGGCGGCACGGGAACACACCGCT
GCGCTGGCTTGCCTGAGCAACGTGCGCCCGGCTGTGGACGCCTTTTTTGACGGCGTAATGGTCATGTGTGACGATGCCGC
CCTACGCCGCAACCGCCTGGCCATGCTTCAGGCCCTGGGCGGTCGTTTTGCCCGTCTGGCGGATTTTGCGGCCTTGCAGA
TGTAG

Upstream 100 bases:

>100_bases
GGCCGGGTGCGGGCTCTGGCGGCGGGCGTTGCCCGTCTGTACGCGGCTCAGCGCGAAGAACTGGGCTATCCCATGCTCAA
AAAGGATGCGAGGTAAAGCC

Downstream 100 bases:

>100_bases
TTGAATCACGCGGCGCGGGCGTTTTTTCGTTCCCGCGCCGCGAATATATCAGCGCGGCGACCGCGAATAACGGAACTGCC
AGCCGATGCGGCCATAAGCG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MATFVLEIGSEELPSRFLAPEEAELFTRFTTALDEAGLEYGSLRVMSTPRRAVVMVENLNPVQIEREEVISGPPVRVAYD
AEGKPTKALEGFVRTNGCALEDVFQQETEKGAYVAVRKRTGGAPASELLAVMCPAIISALPFAKRMRWGNHSLAYARPLR
WILALLDDEIISFEVGPMVSGRETCGHRIHGAGPFSVARAAELPAILADSCGITLDSPKRREIIVEGGNRQAQTVGGKVL
WKDSLLDEVQGLAEHPVPLLADFDPAYLEVPREVLLTSMESHQKSFGIEGADGRLLPHFLTVLNITPEDMSIVKRGWERV
LRARLEDARFFWQADLREGFDHWLQKLDSVIFIGGLGSMGDKTRRLESLCRWLAEKCAPDLAADAARAGRLSKADLVSGL
VGEFDTLQGVMGGIYAARKGESAAVAQAVAEQYLPAGPDSPLPGSMCGALLSLADKADTLAGCFGLGMIPTGAADPNGLR
RCALGIIRIVLDFGLRLDMREVFARAQELYGDRQWKLPPAEALDKLMEFFGARLRNYFMSQGQDTLLVDAALGAGAAQVK
DCGERLEALVDFSREAGFAEAAQTFKRVANILRKQGQAEDIPAQWQAELLHEDAEKALAATLDGLLPELDRMWAAREHTA
ALACLSNVRPAVDAFFDGVMVMCDDAALRRNRLAMLQALGGRFARLADFAALQM

Sequences:

>Translated_694_residues
MATFVLEIGSEELPSRFLAPEEAELFTRFTTALDEAGLEYGSLRVMSTPRRAVVMVENLNPVQIEREEVISGPPVRVAYD
AEGKPTKALEGFVRTNGCALEDVFQQETEKGAYVAVRKRTGGAPASELLAVMCPAIISALPFAKRMRWGNHSLAYARPLR
WILALLDDEIISFEVGPMVSGRETCGHRIHGAGPFSVARAAELPAILADSCGITLDSPKRREIIVEGGNRQAQTVGGKVL
WKDSLLDEVQGLAEHPVPLLADFDPAYLEVPREVLLTSMESHQKSFGIEGADGRLLPHFLTVLNITPEDMSIVKRGWERV
LRARLEDARFFWQADLREGFDHWLQKLDSVIFIGGLGSMGDKTRRLESLCRWLAEKCAPDLAADAARAGRLSKADLVSGL
VGEFDTLQGVMGGIYAARKGESAAVAQAVAEQYLPAGPDSPLPGSMCGALLSLADKADTLAGCFGLGMIPTGAADPNGLR
RCALGIIRIVLDFGLRLDMREVFARAQELYGDRQWKLPPAEALDKLMEFFGARLRNYFMSQGQDTLLVDAALGAGAAQVK
DCGERLEALVDFSREAGFAEAAQTFKRVANILRKQGQAEDIPAQWQAELLHEDAEKALAATLDGLLPELDRMWAAREHTA
ALACLSNVRPAVDAFFDGVMVMCDDAALRRNRLAMLQALGGRFARLADFAALQM
>Mature_693_residues
ATFVLEIGSEELPSRFLAPEEAELFTRFTTALDEAGLEYGSLRVMSTPRRAVVMVENLNPVQIEREEVISGPPVRVAYDA
EGKPTKALEGFVRTNGCALEDVFQQETEKGAYVAVRKRTGGAPASELLAVMCPAIISALPFAKRMRWGNHSLAYARPLRW
ILALLDDEIISFEVGPMVSGRETCGHRIHGAGPFSVARAAELPAILADSCGITLDSPKRREIIVEGGNRQAQTVGGKVLW
KDSLLDEVQGLAEHPVPLLADFDPAYLEVPREVLLTSMESHQKSFGIEGADGRLLPHFLTVLNITPEDMSIVKRGWERVL
RARLEDARFFWQADLREGFDHWLQKLDSVIFIGGLGSMGDKTRRLESLCRWLAEKCAPDLAADAARAGRLSKADLVSGLV
GEFDTLQGVMGGIYAARKGESAAVAQAVAEQYLPAGPDSPLPGSMCGALLSLADKADTLAGCFGLGMIPTGAADPNGLRR
CALGIIRIVLDFGLRLDMREVFARAQELYGDRQWKLPPAEALDKLMEFFGARLRNYFMSQGQDTLLVDAALGAGAAQVKD
CGERLEALVDFSREAGFAEAAQTFKRVANILRKQGQAEDIPAQWQAELLHEDAEKALAATLDGLLPELDRMWAAREHTAA
LACLSNVRPAVDAFFDGVMVMCDDAALRRNRLAMLQALGGRFARLADFAALQM

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=691, Percent_Identity=37.6266280752533, Blast_Score=428, Evalue=1e-121,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75606; Mature: 75475

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATFVLEIGSEELPSRFLAPEEAELFTRFTTALDEAGLEYGSLRVMSTPRRAVVMVENLN
CCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCC
PVQIEREEVISGPPVRVAYDAEGKPTKALEGFVRTNGCALEDVFQQETEKGAYVAVRKRT
CCEEEHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECC
GGAPASELLAVMCPAIISALPFAKRMRWGNHSLAYARPLRWILALLDDEIISFEVGPMVS
CCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCC
GRETCGHRIHGAGPFSVARAAELPAILADSCGITLDSPKRREIIVEGGNRQAQTVGGKVL
CHHHHCCCCCCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCEEEEECCCCCHHHCCCEEE
WKDSLLDEVQGLAEHPVPLLADFDPAYLEVPREVLLTSMESHQKSFGIEGADGRLLPHFL
EHHHHHHHHHHHHHCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
TVLNITPEDMSIVKRGWERVLRARLEDARFFWQADLREGFDHWLQKLDSVIFIGGLGSMG
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
DKTRRLESLCRWLAEKCAPDLAADAARAGRLSKADLVSGLVGEFDTLQGVMGGIYAARKG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ESAAVAQAVAEQYLPAGPDSPLPGSMCGALLSLADKADTLAGCFGLGMIPTGAADPNGLR
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RCALGIIRIVLDFGLRLDMREVFARAQELYGDRQWKLPPAEALDKLMEFFGARLRNYFMS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QGQDTLLVDAALGAGAAQVKDCGERLEALVDFSREAGFAEAAQTFKRVANILRKQGQAED
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
IPAQWQAELLHEDAEKALAATLDGLLPELDRMWAAREHTAALACLSNVRPAVDAFFDGVM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHH
VMCDDAALRRNRLAMLQALGGRFARLADFAALQM
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATFVLEIGSEELPSRFLAPEEAELFTRFTTALDEAGLEYGSLRVMSTPRRAVVMVENLN
CEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCC
PVQIEREEVISGPPVRVAYDAEGKPTKALEGFVRTNGCALEDVFQQETEKGAYVAVRKRT
CCEEEHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECC
GGAPASELLAVMCPAIISALPFAKRMRWGNHSLAYARPLRWILALLDDEIISFEVGPMVS
CCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCC
GRETCGHRIHGAGPFSVARAAELPAILADSCGITLDSPKRREIIVEGGNRQAQTVGGKVL
CHHHHCCCCCCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCEEEEECCCCCHHHCCCEEE
WKDSLLDEVQGLAEHPVPLLADFDPAYLEVPREVLLTSMESHQKSFGIEGADGRLLPHFL
EHHHHHHHHHHHHHCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
TVLNITPEDMSIVKRGWERVLRARLEDARFFWQADLREGFDHWLQKLDSVIFIGGLGSMG
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
DKTRRLESLCRWLAEKCAPDLAADAARAGRLSKADLVSGLVGEFDTLQGVMGGIYAARKG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ESAAVAQAVAEQYLPAGPDSPLPGSMCGALLSLADKADTLAGCFGLGMIPTGAADPNGLR
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RCALGIIRIVLDFGLRLDMREVFARAQELYGDRQWKLPPAEALDKLMEFFGARLRNYFMS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QGQDTLLVDAALGAGAAQVKDCGERLEALVDFSREAGFAEAAQTFKRVANILRKQGQAED
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
IPAQWQAELLHEDAEKALAATLDGLLPELDRMWAAREHTAALACLSNVRPAVDAFFDGVM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHH
VMCDDAALRRNRLAMLQALGGRFARLADFAALQM
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA