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The map label for this gene is rfbF [H]

Identifier: 220904150

GI number: 220904150

Start: 1026130

End: 1026894

Strand: Reverse

Name: rfbF [H]

Synonym: Ddes_0877

Alternate gene names: 220904150

Gene position: 1026894-1026130 (Counterclockwise)

Preceding gene: 220904152

Following gene: 220904149

Centisome position: 35.74

GC content: 52.16

Gene sequence:

>765_bases
ATGAAAGTCATTATTATGTGTGGCGGCAAGGGAACGCGCCTGCGCGAAGAAACCTCGGTCAAACCCAAACCTATGGTTGA
GATCGGCGGACGGCCCGTGCTGTGGCACATCATGTCCATCTATGCGCGTTTCGGCTTCAAGGACTTTGTGCTGCCCCTGG
GCTACAAAGGCCAGGTCATAAAGCAATATTTTCACGATTATAACATCCGCAACACAGATTTTACCGTTGATCTGAAAAGC
GGGTCCATCACCACATATCCCAGCCATATAGAAGACTGGCGCGTCACCCTGTGCGATACGGGTGAAGAAACCCTCAAGGG
CGGACGCCTCAAGCGTGTGGCCAAGTATATCGATACCGACCGTTTCATGGTCACCTATGGCGACGGTGTGGCGGATATAG
ACCTGAACAAACTTATAGAATTTCACAAACAGTCCGGCAGCATAGGAACATTTACCGGCGTGCGTATGCCCTCACGTTTC
GGCACTGTGCGTACCGATAATCAGGGCAGGATACTCTCCTGGGAAGAAAAGCCCGTGCTGGATGAATACATCAACTGCGG
TTTCTTTGTCTTCAAGCGCGAGTTTCTGGACTACCTCAGCGAAGATGAAAACTGCGACCTTGAAAAAGAACCCCTGCAGC
GGCTGGCGGCAGAAGGGCAGCTTTCCATGTATCCACATCCCGGCCAGTGGCAGTGCATGGATACCCTGCGCGACTCCATA
AAGCTCAACGAAATGTGGGATTCCGGCCGTGCATTCTGGGTATAA

Upstream 100 bases:

>100_bases
GAAGGCTGCGGGGCTTTTTTTCCTCACGGCGTTCTGCTACACATGGGCCACACGCGGCGCACGGCATTGGACCTGAGGCG
CGGCGTCAGGAGGCACTTAC

Downstream 100 bases:

>100_bases
ACCCTCTTGACGTGCAAGCGGCGCCGCCTGCATGGCCCGCCGGGCTGTGCAGGCCCCGCACCCGGCCTGTCCTGCGCTGC
ATATGCGCGGCTGCGCGCGG

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKVIIMCGGKGTRLREETSVKPKPMVEIGGRPVLWHIMSIYARFGFKDFVLPLGYKGQVIKQYFHDYNIRNTDFTVDLKS
GSITTYPSHIEDWRVTLCDTGEETLKGGRLKRVAKYIDTDRFMVTYGDGVADIDLNKLIEFHKQSGSIGTFTGVRMPSRF
GTVRTDNQGRILSWEEKPVLDEYINCGFFVFKREFLDYLSEDENCDLEKEPLQRLAAEGQLSMYPHPGQWQCMDTLRDSI
KLNEMWDSGRAFWV

Sequences:

>Translated_254_residues
MKVIIMCGGKGTRLREETSVKPKPMVEIGGRPVLWHIMSIYARFGFKDFVLPLGYKGQVIKQYFHDYNIRNTDFTVDLKS
GSITTYPSHIEDWRVTLCDTGEETLKGGRLKRVAKYIDTDRFMVTYGDGVADIDLNKLIEFHKQSGSIGTFTGVRMPSRF
GTVRTDNQGRILSWEEKPVLDEYINCGFFVFKREFLDYLSEDENCDLEKEPLQRLAAEGQLSMYPHPGQWQCMDTLRDSI
KLNEMWDSGRAFWV
>Mature_254_residues
MKVIIMCGGKGTRLREETSVKPKPMVEIGGRPVLWHIMSIYARFGFKDFVLPLGYKGQVIKQYFHDYNIRNTDFTVDLKS
GSITTYPSHIEDWRVTLCDTGEETLKGGRLKRVAKYIDTDRFMVTYGDGVADIDLNKLIEFHKQSGSIGTFTGVRMPSRF
GTVRTDNQGRILSWEEKPVLDEYINCGFFVFKREFLDYLSEDENCDLEKEPLQRLAAEGQLSMYPHPGQWQCMDTLRDSI
KLNEMWDSGRAFWV

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=235, Percent_Identity=29.3617021276596, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI11761619, Length=235, Percent_Identity=29.3617021276596, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI133931050, Length=251, Percent_Identity=31.4741035856574, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320148, Length=248, Percent_Identity=30.6451612903226, Blast_Score=92, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21355443, Length=244, Percent_Identity=26.6393442622951, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24644084, Length=244, Percent_Identity=26.6393442622951, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 29369; Mature: 29369

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIIMCGGKGTRLREETSVKPKPMVEIGGRPVLWHIMSIYARFGFKDFVLPLGYKGQVI
CEEEEEECCCCCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCHHHEECCCCCHHHH
KQYFHDYNIRNTDFTVDLKSGSITTYPSHIEDWRVTLCDTGEETLKGGRLKRVAKYIDTD
HHHHHHCCCCCCCEEEEECCCCEEECCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHCCC
RFMVTYGDGVADIDLNKLIEFHKQSGSIGTFTGVRMPSRFGTVRTDNQGRILSWEEKPVL
CEEEEECCCEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCEEEECCCCHH
DEYINCGFFVFKREFLDYLSEDENCDLEKEPLQRLAAEGQLSMYPHPGQWQCMDTLRDSI
HHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHE
KLNEMWDSGRAFWV
EEHHHHCCCCEECC
>Mature Secondary Structure
MKVIIMCGGKGTRLREETSVKPKPMVEIGGRPVLWHIMSIYARFGFKDFVLPLGYKGQVI
CEEEEEECCCCCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCHHHEECCCCCHHHH
KQYFHDYNIRNTDFTVDLKSGSITTYPSHIEDWRVTLCDTGEETLKGGRLKRVAKYIDTD
HHHHHHCCCCCCCEEEEECCCCEEECCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHCCC
RFMVTYGDGVADIDLNKLIEFHKQSGSIGTFTGVRMPSRFGTVRTDNQGRILSWEEKPVL
CEEEEECCCEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCEEEECCCCHH
DEYINCGFFVFKREFLDYLSEDENCDLEKEPLQRLAAEGQLSMYPHPGQWQCMDTLRDSI
HHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHE
KLNEMWDSGRAFWV
EEHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]