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The map label for this gene is tolB [H]

Identifier: 220904131

GI number: 220904131

Start: 1005237

End: 1006556

Strand: Reverse

Name: tolB [H]

Synonym: Ddes_0858

Alternate gene names: 220904131

Gene position: 1006556-1005237 (Counterclockwise)

Preceding gene: 220904132

Following gene: 220904130

Centisome position: 35.03

GC content: 59.17

Gene sequence:

>1320_bases
ATGAAAAAACTGCTTCTTCTCCTGGCTCTGGGGCTTTGGCTGGCTCTGGGAAGCGGAGCACAGGCCGCCATGCGCGTGGA
TATTTACGGTCCGGGCCAGAACATCGTCAATCTGGCTCTGGCTGCTCCTCTCAAAGGCCCGCAGGTGCAGGCCACGGGCA
TGGGCGCGGATCTGCAAAAAATTGTTCAGGAAAACCTGAGCTTTTTGCCTTTCATGCGCCTGACGGACCCCAAAGCTGTG
CTTGGCGGCGTGGTGCTGGCCGGATATGAGCCGCCCACTCTGGACTTCAAGCGTTTTCAGCTTGCCGGTTCTGACATTGT
GGTGACCACATTCTGGCCCGAGGGTGACAGTGGCACACGCCCTGTGCAGATTCGCGCATTTGAAACCAATACCGGTGGCC
GCCTGTTCGGTAAGGAATACCCCAAGGTCAACGCCCGCGATCTGCCCGAAGTGGCGGACCGCTTCTGCGCCGACCTGCTT
GAAGCCCTGACAGGCAACGGGGCCTTTTTCCGCTCCACCCTGGCCTTCATCAAAAAGAACGGCAAGATGGACTCCAACGT
CTGGCTGGTCAAGCCTACCGGACGCGACCTGCGCCAGATAACCAATATGCCCGGCGAATCCATGTCGCCTGCATGGTCGC
CGGATGGCCGCTTTGTGGTCTTTACCCAGATAGACCCCAAATCGCATGCCCTTGGCGTGTGGGACCGCTCCACCGGCAAG
GTGCAGCGCATCCGCTTTCCCGGCAACGTGGTCATAGGCCCTGCCTTTATGCCTGACAACAAGGTGGCAGTGGCCCTTTC
CAACGGCAAGTACCCTGTCATTTTCCAGCTCAATCATGTGTTCCAGAAAGAACGGGTGCTTGAGCAGAACGATTCCATCA
ACGTGTCGCCCACTTTTGACAGCACAGGCACCAAGATGGCCTTTACCTCATCCCGTCTGGGCGGCCCGCAGATATTCCTC
AAGGATCTCGGCAGTGGGTCCGTTACCCGCGTGAGCAAAAACGGCACGTACAATTCCGAGGCCAACCTGTCCCCTGACGG
CACGCTTGTGGTCTATAGCCGCATGACAGAATACGGCCACCGCATTTTCGTGCAGGACATGCTCACCGGCACCGAACGCC
AGATCAGCTTTGGTCCCGGCAGCGACGAACAACCCTCTTTCTGTGCGGACAGCTACTTTATCGCCTTCACCTCCTCGCGT
GGAGGCGGCCGCGGCATCTACCTGACCACGCGGCATGGCGGCGACGCCAAAAGAGTGCCCACAGGCGGCGGAGACTCGTC
TTTCCCCCGGTGGGGCATGCCCGGACAGCAAAAGCAATAA

Upstream 100 bases:

>100_bases
CAGCATTCCGCCGCCACCCGGGGACCTTGGCGCAACGCTGGACATAACTCTTACACTGGTGCGCCGCTAGCGCGCCGACC
CAACAAGGACTGCTGACGCC

Downstream 100 bases:

>100_bases
ATTCAGTATAATAAGATTTAAAAAAATGTTTTTTACCCGGTTTGTTGCTTTTTTCGATCCAACCGGGTAAAAAATATTTT
ACGAAAGTGCGCCTAACCCC

Product: WD40 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKKLLLLLALGLWLALGSGAQAAMRVDIYGPGQNIVNLALAAPLKGPQVQATGMGADLQKIVQENLSFLPFMRLTDPKAV
LGGVVLAGYEPPTLDFKRFQLAGSDIVVTTFWPEGDSGTRPVQIRAFETNTGGRLFGKEYPKVNARDLPEVADRFCADLL
EALTGNGAFFRSTLAFIKKNGKMDSNVWLVKPTGRDLRQITNMPGESMSPAWSPDGRFVVFTQIDPKSHALGVWDRSTGK
VQRIRFPGNVVIGPAFMPDNKVAVALSNGKYPVIFQLNHVFQKERVLEQNDSINVSPTFDSTGTKMAFTSSRLGGPQIFL
KDLGSGSVTRVSKNGTYNSEANLSPDGTLVVYSRMTEYGHRIFVQDMLTGTERQISFGPGSDEQPSFCADSYFIAFTSSR
GGGRGIYLTTRHGGDAKRVPTGGGDSSFPRWGMPGQQKQ

Sequences:

>Translated_439_residues
MKKLLLLLALGLWLALGSGAQAAMRVDIYGPGQNIVNLALAAPLKGPQVQATGMGADLQKIVQENLSFLPFMRLTDPKAV
LGGVVLAGYEPPTLDFKRFQLAGSDIVVTTFWPEGDSGTRPVQIRAFETNTGGRLFGKEYPKVNARDLPEVADRFCADLL
EALTGNGAFFRSTLAFIKKNGKMDSNVWLVKPTGRDLRQITNMPGESMSPAWSPDGRFVVFTQIDPKSHALGVWDRSTGK
VQRIRFPGNVVIGPAFMPDNKVAVALSNGKYPVIFQLNHVFQKERVLEQNDSINVSPTFDSTGTKMAFTSSRLGGPQIFL
KDLGSGSVTRVSKNGTYNSEANLSPDGTLVVYSRMTEYGHRIFVQDMLTGTERQISFGPGSDEQPSFCADSYFIAFTSSR
GGGRGIYLTTRHGGDAKRVPTGGGDSSFPRWGMPGQQKQ
>Mature_439_residues
MKKLLLLLALGLWLALGSGAQAAMRVDIYGPGQNIVNLALAAPLKGPQVQATGMGADLQKIVQENLSFLPFMRLTDPKAV
LGGVVLAGYEPPTLDFKRFQLAGSDIVVTTFWPEGDSGTRPVQIRAFETNTGGRLFGKEYPKVNARDLPEVADRFCADLL
EALTGNGAFFRSTLAFIKKNGKMDSNVWLVKPTGRDLRQITNMPGESMSPAWSPDGRFVVFTQIDPKSHALGVWDRSTGK
VQRIRFPGNVVIGPAFMPDNKVAVALSNGKYPVIFQLNHVFQKERVLEQNDSINVSPTFDSTGTKMAFTSSRLGGPQIFL
KDLGSGSVTRVSKNGTYNSEANLSPDGTLVVYSRMTEYGHRIFVQDMLTGTERQISFGPGSDEQPSFCADSYFIAFTSSR
GGGRGIYLTTRHGGDAKRVPTGGGDSSFPRWGMPGQQKQ

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=303, Percent_Identity=27.3927392739274, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40 [H]

EC number: NA

Molecular weight: Translated: 47699; Mature: 47699

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLLLLALGLWLALGSGAQAAMRVDIYGPGQNIVNLALAAPLKGPQVQATGMGADLQK
CHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHEEEEEECCCCCCEEEEECCCHHHHH
IVQENLSFLPFMRLTDPKAVLGGVVLAGYEPPTLDFKRFQLAGSDIVVTTFWPEGDSGTR
HHHHCCCCCCEEECCCCHHHHCCEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCC
PVQIRAFETNTGGRLFGKEYPKVNARDLPEVADRFCADLLEALTGNGAFFRSTLAFIKKN
CEEEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
GKMDSNVWLVKPTGRDLRQITNMPGESMSPAWSPDGRFVVFTQIDPKSHALGVWDRSTGK
CCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCC
VQRIRFPGNVVIGPAFMPDNKVAVALSNGKYPVIFQLNHVFQKERVLEQNDSINVSPTFD
EEEEECCCCEEECCEECCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHCCCCEEEEECCC
STGTKMAFTSSRLGGPQIFLKDLGSGSVTRVSKNGTYNSEANLSPDGTLVVYSRMTEYGH
CCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEHHHCCC
RIFVQDMLTGTERQISFGPGSDEQPSFCADSYFIAFTSSRGGGRGIYLTTRHGGDAKRVP
EEEEEHHHCCCCEEEEECCCCCCCCCEECCCEEEEEECCCCCCCEEEEEEECCCCCCCCC
TGGGDSSFPRWGMPGQQKQ
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKLLLLLALGLWLALGSGAQAAMRVDIYGPGQNIVNLALAAPLKGPQVQATGMGADLQK
CHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHEEEEEECCCCCCEEEEECCCHHHHH
IVQENLSFLPFMRLTDPKAVLGGVVLAGYEPPTLDFKRFQLAGSDIVVTTFWPEGDSGTR
HHHHCCCCCCEEECCCCHHHHCCEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCC
PVQIRAFETNTGGRLFGKEYPKVNARDLPEVADRFCADLLEALTGNGAFFRSTLAFIKKN
CEEEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
GKMDSNVWLVKPTGRDLRQITNMPGESMSPAWSPDGRFVVFTQIDPKSHALGVWDRSTGK
CCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCC
VQRIRFPGNVVIGPAFMPDNKVAVALSNGKYPVIFQLNHVFQKERVLEQNDSINVSPTFD
EEEEECCCCEEECCEECCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHCCCCEEEEECCC
STGTKMAFTSSRLGGPQIFLKDLGSGSVTRVSKNGTYNSEANLSPDGTLVVYSRMTEYGH
CCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEHHHCCC
RIFVQDMLTGTERQISFGPGSDEQPSFCADSYFIAFTSSRGGGRGIYLTTRHGGDAKRVP
EEEEEHHHCCCCEEEEECCCCCCCCCEECCCEEEEEECCCCCCCEEEEEEECCCCCCCCC
TGGGDSSFPRWGMPGQQKQ
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA