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The map label for this gene is hprA [H]

Identifier: 220904129

GI number: 220904129

Start: 1003354

End: 1004325

Strand: Reverse

Name: hprA [H]

Synonym: Ddes_0856

Alternate gene names: 220904129

Gene position: 1004325-1003354 (Counterclockwise)

Preceding gene: 220904130

Following gene: 220904128

Centisome position: 34.95

GC content: 57.51

Gene sequence:

>972_bases
ATGAAGATTGTCATTCTGGATGGCGGGGTGCTCAACCCCGGCGATGTTGATTGGGGGCCTGTCACCGCTTTGGGCGATGT
AAGCATATATGACGAAACCAGCAAGGATCAGCTGGCCGAGCGCGCCAGGGGTGCGGATGTGCTCCTCACCAACAAAACCC
CCCTGCTCAAGGAAGATCTGCCCGCCCTTGAAAGTGTGCGTATGGTAGGCGTACTGGCTACAGGATATAACATCATCGAT
ACCGAAGCGCTGGCCCAGCGGGGCATACCCGTATGCAACGTGGTGGCTTACGGGGTAAGTGATGTGGCCCAGCATGCCAT
GGCCCTGTTGCTTGAATTGTGCCGTCACACCAGCCTGCATACCCAGAGCGTCAAAAACGGCGACTGGCAAAAATCAAAAC
AGTGGTGCTACTGGAAAGTACCTCCGGTTTGCCTTGAAGGCATGACCATGGGACTTATTGGTTTTGGCTCCATTGGCCGC
CGCATGGGCGAGCTGGCGCATGCCTTCGGCATGAATGTTCTTGCCCAATGCCGCACCCCAAAGAATCCACCCGCGTACAG
CCCCTTTGCTTTCGCCACGCTGGAGCATCTTATCTGCGCTTCGGATGTCATTTCGCTGCACTGCCCCCTGACGAAAGAAA
CGCAGCACATCATCAATGCCAAGACTCTGTCCAACATGCGCAAGGGTGCCATTCTGCTCAATACCTCCCGTGGTCCGCTG
GTCGATGAAGCCGCCGCTGCTGCCGCCCTTAAAAGCGGCCAGTTGGGTGGACTCGGCACAGACGTACTCTCGGAGGAGCC
GCCAAGCCCCGATAACCCCCTGCTCTCTGCCCCCAATACGCTCATCACCCCACATATTGCCTGGGCCACTACCAGGGCAA
GGCAGAACATCATTGATCTCACGGCGGAAAATATCCGTCGCTGGCAGGCCGGCACACCCGTCAACGTAGTCAACGGGGTG
AGCAGCGTCTAA

Upstream 100 bases:

>100_bases
GCGCGCCACATTTCTGCGTTCATGTGTTTTGTTCGCGCGGGCAGAGGCCTTGCGAAGGGTACTGGCTCAGGCTACACTGC
GCCTAAGCGAGGATATTCTC

Downstream 100 bases:

>100_bases
TCATGCATATTGACATCCACACCCACGCCTTTCATCCCAAGATTGCGCACAAGGCGGTGGAACATCTGAACGGGCACTAC
GCCGTTGCCTGTGCGGGCAA

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKIVILDGGVLNPGDVDWGPVTALGDVSIYDETSKDQLAERARGADVLLTNKTPLLKEDLPALESVRMVGVLATGYNIID
TEALAQRGIPVCNVVAYGVSDVAQHAMALLLELCRHTSLHTQSVKNGDWQKSKQWCYWKVPPVCLEGMTMGLIGFGSIGR
RMGELAHAFGMNVLAQCRTPKNPPAYSPFAFATLEHLICASDVISLHCPLTKETQHIINAKTLSNMRKGAILLNTSRGPL
VDEAAAAAALKSGQLGGLGTDVLSEEPPSPDNPLLSAPNTLITPHIAWATTRARQNIIDLTAENIRRWQAGTPVNVVNGV
SSV

Sequences:

>Translated_323_residues
MKIVILDGGVLNPGDVDWGPVTALGDVSIYDETSKDQLAERARGADVLLTNKTPLLKEDLPALESVRMVGVLATGYNIID
TEALAQRGIPVCNVVAYGVSDVAQHAMALLLELCRHTSLHTQSVKNGDWQKSKQWCYWKVPPVCLEGMTMGLIGFGSIGR
RMGELAHAFGMNVLAQCRTPKNPPAYSPFAFATLEHLICASDVISLHCPLTKETQHIINAKTLSNMRKGAILLNTSRGPL
VDEAAAAAALKSGQLGGLGTDVLSEEPPSPDNPLLSAPNTLITPHIAWATTRARQNIIDLTAENIRRWQAGTPVNVVNGV
SSV
>Mature_323_residues
MKIVILDGGVLNPGDVDWGPVTALGDVSIYDETSKDQLAERARGADVLLTNKTPLLKEDLPALESVRMVGVLATGYNIID
TEALAQRGIPVCNVVAYGVSDVAQHAMALLLELCRHTSLHTQSVKNGDWQKSKQWCYWKVPPVCLEGMTMGLIGFGSIGR
RMGELAHAFGMNVLAQCRTPKNPPAYSPFAFATLEHLICASDVISLHCPLTKETQHIINAKTLSNMRKGAILLNTSRGPL
VDEAAAAAALKSGQLGGLGTDVLSEEPPSPDNPLLSAPNTLITPHIAWATTRARQNIIDLTAENIRRWQAGTPVNVVNGV
SSV

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=301, Percent_Identity=33.2225913621262, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI4557497, Length=301, Percent_Identity=33.2225913621262, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI145580578, Length=304, Percent_Identity=32.5657894736842, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4557499, Length=304, Percent_Identity=32.5657894736842, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI145580575, Length=300, Percent_Identity=32.3333333333333, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI23308577, Length=269, Percent_Identity=30.8550185873606, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI6912396, Length=256, Percent_Identity=30.859375, Blast_Score=114, Evalue=8e-26,
Organism=Escherichia coli, GI87082289, Length=274, Percent_Identity=33.5766423357664, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787645, Length=297, Percent_Identity=31.3131313131313, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1789279, Length=289, Percent_Identity=32.5259515570934, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87081824, Length=206, Percent_Identity=30.0970873786408, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788660, Length=267, Percent_Identity=27.3408239700375, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25147481, Length=303, Percent_Identity=30.6930693069307, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17532191, Length=297, Percent_Identity=27.9461279461279, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324055, Length=324, Percent_Identity=28.3950617283951, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6322116, Length=298, Percent_Identity=28.5234899328859, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=298, Percent_Identity=28.5234899328859, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6324964, Length=242, Percent_Identity=29.7520661157025, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324980, Length=179, Percent_Identity=29.0502793296089, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6321253, Length=167, Percent_Identity=30.5389221556886, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24646446, Length=307, Percent_Identity=32.8990228013029, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24646448, Length=307, Percent_Identity=32.8990228013029, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24646452, Length=307, Percent_Identity=32.8990228013029, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24646450, Length=307, Percent_Identity=32.8990228013029, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI62472511, Length=301, Percent_Identity=33.2225913621262, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24585514, Length=287, Percent_Identity=28.5714285714286, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI28574282, Length=287, Percent_Identity=28.5714285714286, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI45551003, Length=287, Percent_Identity=28.5714285714286, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI28574284, Length=287, Percent_Identity=28.5714285714286, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI45552429, Length=287, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI28574286, Length=287, Percent_Identity=26.4808362369338, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28571528, Length=289, Percent_Identity=32.5259515570934, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24585516, Length=285, Percent_Identity=25.6140350877193, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19921140, Length=283, Percent_Identity=26.8551236749117, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34597; Mature: 34597

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVILDGGVLNPGDVDWGPVTALGDVSIYDETSKDQLAERARGADVLLTNKTPLLKEDL
CEEEEEECCCCCCCCCCCCCCEEECCEEECCCCCHHHHHHHHCCCEEEEECCCCCHHHHC
PALESVRMVGVLATGYNIIDTEALAQRGIPVCNVVAYGVSDVAQHAMALLLELCRHTSLH
HHHHHHHHHHHHCCCCCEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TQSVKNGDWQKSKQWCYWKVPPVCLEGMTMGLIGFGSIGRRMGELAHAFGMNVLAQCRTP
HHHCCCCCCCCCCCCEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KNPPAYSPFAFATLEHLICASDVISLHCPLTKETQHIINAKTLSNMRKGAILLNTSRGPL
CCCCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCC
VDEAAAAAALKSGQLGGLGTDVLSEEPPSPDNPLLSAPNTLITPHIAWATTRARQNIIDL
HHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHH
TAENIRRWQAGTPVNVVNGVSSV
HHHHHHHHCCCCCHHHHCCCCCC
>Mature Secondary Structure
MKIVILDGGVLNPGDVDWGPVTALGDVSIYDETSKDQLAERARGADVLLTNKTPLLKEDL
CEEEEEECCCCCCCCCCCCCCEEECCEEECCCCCHHHHHHHHCCCEEEEECCCCCHHHHC
PALESVRMVGVLATGYNIIDTEALAQRGIPVCNVVAYGVSDVAQHAMALLLELCRHTSLH
HHHHHHHHHHHHCCCCCEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TQSVKNGDWQKSKQWCYWKVPPVCLEGMTMGLIGFGSIGRRMGELAHAFGMNVLAQCRTP
HHHCCCCCCCCCCCCEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KNPPAYSPFAFATLEHLICASDVISLHCPLTKETQHIINAKTLSNMRKGAILLNTSRGPL
CCCCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCC
VDEAAAAAALKSGQLGGLGTDVLSEEPPSPDNPLLSAPNTLITPHIAWATTRARQNIIDL
HHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHH
TAENIRRWQAGTPVNVVNGVSSV
HHHHHHHHCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]