Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 220904026

GI number: 220904026

Start: 886397

End: 888079

Strand: Direct

Name: nadE [H]

Synonym: Ddes_0752

Alternate gene names: 220904026

Gene position: 886397-888079 (Clockwise)

Preceding gene: 220904025

Following gene: 220904027

Centisome position: 30.85

GC content: 61.44

Gene sequence:

>1683_bases
ATGAAGATAGCCCTTTTACAGTGCAACAGCGTTACCGGTGATGTCGCCGGAAACATGCAGCGCATTCTTGAAGCAAGCCG
TCAGGCTGCGGCTGCCGGGGCCGAACTGTGCGTTACGCCGGAGCTGGCCCTGTGCGGCGTGGCCCCCGGCCATTATCTCT
GCGCTGAAGACTTCGCGGCAGGATGCCGCACGGCCCTGGACCTTCTCGCCCTGGAACTCAGGGATGGTCCGGCCCTGCTG
GTGGGCGCGCCCGTACCCAGTGTTTACGCTTCGGGGCTGCTTTCCAATGCTGCCGTTCTTGTTGACAAGGGAAGCTGGCA
GGTGGCCTCACGCAAGGTCTATCAGGGCGGCGCGCAGGGGATGGGGCCAGACACGGACGACAGCCGTTATTTTGACAGGG
GCATCTCCTGCGGTATACTCACCATCGGTGGCTGGCGCATGGGGGTGGTGCTGTGCGAAGACGCACGCAGCGAAGAAGAG
GCTTTCTGGAAAATACGTTACGCCAGCGGGCATAATCCGCTTATGGAGCTGGTGCAGCGGGGCGTTGACGCTCTCGTTCA
CATGACTGCCGCGCCGTACAGCGAGGGGGCGCAGGCCGCGGGCGAGCACATGCTTTCACATGTGGCGGCCCGGCATCATG
TGCATCTTTTTTCCGTCAATCTTGTGGGAGGCAACGACAGCCGCGTGTACAGCGGCCAGAGCCTCGCCTTTGACCCCACG
GGCCAGTTGCTGGCGCGCGGCAAGGCTTTTGCCGAAGACGTCCTGGTGGTGGAAACCGCCGGAAGCGCCCCGGGCGGTGC
ATCGGGCGGCAAGGCTCCCGAGCCGTTGTCCAGCTGCGTTGAAGAAAATCTCTGGCACGCCCTTACCCTCGGCACGCGCG
ACTTTGTGCGCAAATGTGGCGCAGAAAAGGTAATCGTGGGCCTTTCGGGCGGTATGGATTCTTCGCTGGTGTGCTGCGTG
GCCGTGGAGGCGCTGGGGGCAGAAAACGTCACCGGCGTACTCATGCCGTCGCCTTACAGCAGTGAAGGTTCTGTCAGCGA
TGCCCATAGCCTGGCGCAAAATCTGGGCATACGTACCGTCACCCTGCCCATAGAATCTGTCATGAAGGTTTTTGAACAGG
CCCTTGCTCCCGGGCTGGAGCTTTTCACATCCCGCCCGGGCGACACCACGTTTGAAAATCTTCAGGCACGCATTCGCGGT
GTGGTTCTGTCATCTCTGGCAAACAGGGCGGGCGCTCTTGTACTCAACACGGGGAATAAAAGTGAAGCCGCCATGGGTTA
CAGCACCCTGTACGGCGACACTGTGGGCGCGCTTGCCGTCATTGGCGACCTGACAAAAACGCAGACCTATGCCGTGGCCC
GGTGGTACAATGAGTACCGTCAGGCAGAGGTGATTCCGCAACAGGTTTTTGGCAAGGCTCCTTCCGCAGAACTGCGGCCC
GGCCAGAAGGATGAAGACAGCCTGCCGCCTTACGGGCAGCTTGATCCTGTACTCGAAGAGCTGCTGTTGCCCGGTGCTTC
TTCTGCTGCGGCCAATATTTCACCCTTGCGGCTTGAGGTGCGGAACAGGCTTTTTGCGGCGGAATTCAAGCGCAGGCAGG
AACCGTTGGCGCTTTTTGTAAGCCGCGTGCCTTTTGGTGCGGGCTGGCAGGCCCCGGTGGCGGGGCGCTATCGCCTGCCC
TGA

Upstream 100 bases:

>100_bases
CCCCCCTGGAAGAGGACCCGGCCATCACCCGCAGGGTGGATGCGCTGGCTGCTCCGGGCGGGCCTTTGCACGGATATATC
TAACTCGGGGGAGGCTGCGG

Downstream 100 bases:

>100_bases
AGCTGAGCAGCTTTGAAATACTTTATGTTTCAAAGCTGTCATTCTGCCGGAAAATGCAATTTCGGCAGAATTCACGCCGC
GTTACGCCACGCTGTACTCT

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MKIALLQCNSVTGDVAGNMQRILEASRQAAAAGAELCVTPELALCGVAPGHYLCAEDFAAGCRTALDLLALELRDGPALL
VGAPVPSVYASGLLSNAAVLVDKGSWQVASRKVYQGGAQGMGPDTDDSRYFDRGISCGILTIGGWRMGVVLCEDARSEEE
AFWKIRYASGHNPLMELVQRGVDALVHMTAAPYSEGAQAAGEHMLSHVAARHHVHLFSVNLVGGNDSRVYSGQSLAFDPT
GQLLARGKAFAEDVLVVETAGSAPGGASGGKAPEPLSSCVEENLWHALTLGTRDFVRKCGAEKVIVGLSGGMDSSLVCCV
AVEALGAENVTGVLMPSPYSSEGSVSDAHSLAQNLGIRTVTLPIESVMKVFEQALAPGLELFTSRPGDTTFENLQARIRG
VVLSSLANRAGALVLNTGNKSEAAMGYSTLYGDTVGALAVIGDLTKTQTYAVARWYNEYRQAEVIPQQVFGKAPSAELRP
GQKDEDSLPPYGQLDPVLEELLLPGASSAAANISPLRLEVRNRLFAAEFKRRQEPLALFVSRVPFGAGWQAPVAGRYRLP

Sequences:

>Translated_560_residues
MKIALLQCNSVTGDVAGNMQRILEASRQAAAAGAELCVTPELALCGVAPGHYLCAEDFAAGCRTALDLLALELRDGPALL
VGAPVPSVYASGLLSNAAVLVDKGSWQVASRKVYQGGAQGMGPDTDDSRYFDRGISCGILTIGGWRMGVVLCEDARSEEE
AFWKIRYASGHNPLMELVQRGVDALVHMTAAPYSEGAQAAGEHMLSHVAARHHVHLFSVNLVGGNDSRVYSGQSLAFDPT
GQLLARGKAFAEDVLVVETAGSAPGGASGGKAPEPLSSCVEENLWHALTLGTRDFVRKCGAEKVIVGLSGGMDSSLVCCV
AVEALGAENVTGVLMPSPYSSEGSVSDAHSLAQNLGIRTVTLPIESVMKVFEQALAPGLELFTSRPGDTTFENLQARIRG
VVLSSLANRAGALVLNTGNKSEAAMGYSTLYGDTVGALAVIGDLTKTQTYAVARWYNEYRQAEVIPQQVFGKAPSAELRP
GQKDEDSLPPYGQLDPVLEELLLPGASSAAANISPLRLEVRNRLFAAEFKRRQEPLALFVSRVPFGAGWQAPVAGRYRLP
>Mature_560_residues
MKIALLQCNSVTGDVAGNMQRILEASRQAAAAGAELCVTPELALCGVAPGHYLCAEDFAAGCRTALDLLALELRDGPALL
VGAPVPSVYASGLLSNAAVLVDKGSWQVASRKVYQGGAQGMGPDTDDSRYFDRGISCGILTIGGWRMGVVLCEDARSEEE
AFWKIRYASGHNPLMELVQRGVDALVHMTAAPYSEGAQAAGEHMLSHVAARHHVHLFSVNLVGGNDSRVYSGQSLAFDPT
GQLLARGKAFAEDVLVVETAGSAPGGASGGKAPEPLSSCVEENLWHALTLGTRDFVRKCGAEKVIVGLSGGMDSSLVCCV
AVEALGAENVTGVLMPSPYSSEGSVSDAHSLAQNLGIRTVTLPIESVMKVFEQALAPGLELFTSRPGDTTFENLQARIRG
VVLSSLANRAGALVLNTGNKSEAAMGYSTLYGDTVGALAVIGDLTKTQTYAVARWYNEYRQAEVIPQQVFGKAPSAELRP
GQKDEDSLPPYGQLDPVLEELLLPGASSAAANISPLRLEVRNRLFAAEFKRRQEPLALFVSRVPFGAGWQAPVAGRYRLP

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59081; Mature: 59081

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALLQCNSVTGDVAGNMQRILEASRQAAAAGAELCVTPELALCGVAPGHYLCAEDFAA
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHEECCCCCCEEEHHHHHH
GCRTALDLLALELRDGPALLVGAPVPSVYASGLLSNAAVLVDKGSWQVASRKVYQGGAQG
HHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCC
MGPDTDDSRYFDRGISCGILTIGGWRMGVVLCEDARSEEEAFWKIRYASGHNPLMELVQR
CCCCCCCCHHHHCCCCEEEEEECCEEEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHH
GVDALVHMTAAPYSEGAQAAGEHMLSHVAARHHVHLFSVNLVGGNDSRVYSGQSLAFDPT
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCCEEECCCCEEECCC
GQLLARGKAFAEDVLVVETAGSAPGGASGGKAPEPLSSCVEENLWHALTLGTRDFVRKCG
HHHHHCCHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCHHHHHHCC
AEKVIVGLSGGMDSSLVCCVAVEALGAENVTGVLMPSPYSSEGSVSDAHSLAQNLGIRTV
CCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCEEE
TLPIESVMKVFEQALAPGLELFTSRPGDTTFENLQARIRGVVLSSLANRAGALVLNTGNK
EECHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
SEAAMGYSTLYGDTVGALAVIGDLTKTQTYAVARWYNEYRQAEVIPQQVFGKAPSAELRP
CCHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCC
GQKDEDSLPPYGQLDPVLEELLLPGASSAAANISPLRLEVRNRLFAAEFKRRQEPLALFV
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
SRVPFGAGWQAPVAGRYRLP
HHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIALLQCNSVTGDVAGNMQRILEASRQAAAAGAELCVTPELALCGVAPGHYLCAEDFAA
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHEECCCCCCEEEHHHHHH
GCRTALDLLALELRDGPALLVGAPVPSVYASGLLSNAAVLVDKGSWQVASRKVYQGGAQG
HHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCC
MGPDTDDSRYFDRGISCGILTIGGWRMGVVLCEDARSEEEAFWKIRYASGHNPLMELVQR
CCCCCCCCHHHHCCCCEEEEEECCEEEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHH
GVDALVHMTAAPYSEGAQAAGEHMLSHVAARHHVHLFSVNLVGGNDSRVYSGQSLAFDPT
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCCEEECCCCEEECCC
GQLLARGKAFAEDVLVVETAGSAPGGASGGKAPEPLSSCVEENLWHALTLGTRDFVRKCG
HHHHHCCHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCHHHHHHCC
AEKVIVGLSGGMDSSLVCCVAVEALGAENVTGVLMPSPYSSEGSVSDAHSLAQNLGIRTV
CCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCEEE
TLPIESVMKVFEQALAPGLELFTSRPGDTTFENLQARIRGVVLSSLANRAGALVLNTGNK
EECHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
SEAAMGYSTLYGDTVGALAVIGDLTKTQTYAVARWYNEYRQAEVIPQQVFGKAPSAELRP
CCHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCC
GQKDEDSLPPYGQLDPVLEELLLPGASSAAANISPLRLEVRNRLFAAEFKRRQEPLALFV
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
SRVPFGAGWQAPVAGRYRLP
HHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]