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The map label for this gene is tig

Identifier: 220903964

GI number: 220903964

Start: 819321

End: 820679

Strand: Direct

Name: tig

Synonym: Ddes_0690

Alternate gene names: 220903964

Gene position: 819321-820679 (Clockwise)

Preceding gene: 220903963

Following gene: 220903965

Centisome position: 28.51

GC content: 57.32

Gene sequence:

>1359_bases
GTGGAATATAGCGCTGAAGACATTTCGCCGGTCAGAAAAAAAGTCGTCATCACTACGGAACCCCAGGAAGTTGAAGCTTC
CATCATGGGCACGATCGCCCTGTACAAGACTTCCGTACAAGTTGACGGTTTCCGCAAGGGCAAGGTTCCGGCCTCGGTCA
TCGAGCAGCGCTTTCGCGACAAGATTTACGAAGAAGCCCGTCAGGACCTCATCAACGTGCACATCAATGACGTGATGCAG
AAGCTCGACGTCATGCCGCTGGCCGGTCTTGATGTAGACACGCCCGACGCTTTCGAGCGCGGCAAGGGCTTTGCCTATAC
CATCGAATTTGAAGTGCTGCCCACGTTTGATCTGCCCCCTTACGAAGGTATGGATGTGGAGCAGGAAAAAGTTGTGGTGG
ACGAAAAAGAAGTGCAGGAAGTGCTCGATCGCATCCTGCGCGACCGCGCCCAGCTTGTGCCCGTGGAGGGCAAAGGCCCC
GCTGTGGATGGTCAGATAGCCACCGTTGACTTTGCCGCTTTTGAAAACGGCGAACCCGTGGAAGGCGTCAAGGCCGAATC
CTTTGATCTGGCCCTGGGCGAGCGCCAGGCTCTGGAAGATTTTGAAGCTCTGGTCAAGACCATTCCTTATGGCGAAGAAG
GTGAGGGCGAGATTCACTTTCCCGAAGACTTCATTGCCAAGGATCTGGCTGGCAAGACCGTGACCATGAAGGTCAAGGTG
CATGCCATCAAGGAACGCAAGCTGCCCGCGCTTGACGATGAACTGGCCAAGAGCCTTGGCCTTGAAAGCGTTGAAAAGCT
GAAAGAAGCCATCACCAAGAGCTACACTGAAAGCCGCACCGGCCTGAACAGGAGCGCGGCCCAGAAGAGCCTGCTGGACG
GCCTGCTCAAGATGGTTGCATTTGAGCTGCCCCCGAGCCTGGTGGAAACCCAGATGAACACGCTGTTGGCCGACATGGCC
GGCCGCCTGGAACGTCAGGGCCGCAGCCTCGAAAGCCTCGGCAAAAGCATGGAAGACCTGCGCAAGGACATGCAGCCCCA
GGCTGACGAACTGGCCCGCGCCCAGGTGCTGCTGCTTTCTGTTGCCAAAAAAGAAGGTCTGGACGTTACCGACCACGAAG
TTACCACGCAGATTTACCAGCTTGCCATGCGCACCGGTGAAGACTTCAAGGCCCTGCGCGAACAGTATGAGCGCTCGGGC
ATGATCTTTGTGCTGCGCGACCGCATGCTGGCGGACAAGGCCATGGATCTCATCTACGCCAAGGCCAATGTCAAGGAAGT
GGAGCCAAAAGCTCCCGAAACCGGAGCAGAGGCCGATGCCGCGCCGGGCGCATCCGCCAGCGCTCAGGAAAGCAAATAA

Upstream 100 bases:

>100_bases
GAATTGGTAGACGCACTGGACTTAGAATCCAGCACCATAGGTATGGGGGTTCAACTCCCCCCTCTCGCACCATCACGCCC
ACCCAGAAAAGGAGTCCCTC

Downstream 100 bases:

>100_bases
TAATCAGGGAAGGCGGCCTCTGGGCCGCCTTTTGCTTTTTTCGCGGCTCGATGCCGCATATATCGGCCCCCGGGCCATGT
CAGTGAGGCTTTCATGTCGC

Product: trigger factor

Products: peptidylproline (omega=0)

Alternate protein names: TF

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MEYSAEDISPVRKKVVITTEPQEVEASIMGTIALYKTSVQVDGFRKGKVPASVIEQRFRDKIYEEARQDLINVHINDVMQ
KLDVMPLAGLDVDTPDAFERGKGFAYTIEFEVLPTFDLPPYEGMDVEQEKVVVDEKEVQEVLDRILRDRAQLVPVEGKGP
AVDGQIATVDFAAFENGEPVEGVKAESFDLALGERQALEDFEALVKTIPYGEEGEGEIHFPEDFIAKDLAGKTVTMKVKV
HAIKERKLPALDDELAKSLGLESVEKLKEAITKSYTESRTGLNRSAAQKSLLDGLLKMVAFELPPSLVETQMNTLLADMA
GRLERQGRSLESLGKSMEDLRKDMQPQADELARAQVLLLSVAKKEGLDVTDHEVTTQIYQLAMRTGEDFKALREQYERSG
MIFVLRDRMLADKAMDLIYAKANVKEVEPKAPETGAEADAAPGASASAQESK

Sequences:

>Translated_452_residues
MEYSAEDISPVRKKVVITTEPQEVEASIMGTIALYKTSVQVDGFRKGKVPASVIEQRFRDKIYEEARQDLINVHINDVMQ
KLDVMPLAGLDVDTPDAFERGKGFAYTIEFEVLPTFDLPPYEGMDVEQEKVVVDEKEVQEVLDRILRDRAQLVPVEGKGP
AVDGQIATVDFAAFENGEPVEGVKAESFDLALGERQALEDFEALVKTIPYGEEGEGEIHFPEDFIAKDLAGKTVTMKVKV
HAIKERKLPALDDELAKSLGLESVEKLKEAITKSYTESRTGLNRSAAQKSLLDGLLKMVAFELPPSLVETQMNTLLADMA
GRLERQGRSLESLGKSMEDLRKDMQPQADELARAQVLLLSVAKKEGLDVTDHEVTTQIYQLAMRTGEDFKALREQYERSG
MIFVLRDRMLADKAMDLIYAKANVKEVEPKAPETGAEADAAPGASASAQESK
>Mature_452_residues
MEYSAEDISPVRKKVVITTEPQEVEASIMGTIALYKTSVQVDGFRKGKVPASVIEQRFRDKIYEEARQDLINVHINDVMQ
KLDVMPLAGLDVDTPDAFERGKGFAYTIEFEVLPTFDLPPYEGMDVEQEKVVVDEKEVQEVLDRILRDRAQLVPVEGKGP
AVDGQIATVDFAAFENGEPVEGVKAESFDLALGERQALEDFEALVKTIPYGEEGEGEIHFPEDFIAKDLAGKTVTMKVKV
HAIKERKLPALDDELAKSLGLESVEKLKEAITKSYTESRTGLNRSAAQKSLLDGLLKMVAFELPPSLVETQMNTLLADMA
GRLERQGRSLESLGKSMEDLRKDMQPQADELARAQVLLLSVAKKEGLDVTDHEVTTQIYQLAMRTGEDFKALREQYERSG
MIFVLRDRMLADKAMDLIYAKANVKEVEPKAPETGAEADAAPGASASAQESK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=431, Percent_Identity=28.3062645011601, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_DESDA (B8IYM1)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479276.1
- GeneID:   7284364
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0690
- HOGENOM:   HBG458144
- ProtClustDB:   CLSK705168
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: 5.2.1.8

Molecular weight: Translated: 50084; Mature: 50084

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYSAEDISPVRKKVVITTEPQEVEASIMGTIALYKTSVQVDGFRKGKVPASVIEQRFRD
CCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHH
KIYEEARQDLINVHINDVMQKLDVMPLAGLDVDTPDAFERGKGFAYTIEFEVLPTFDLPP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEEEEEEEEECCCCCC
YEGMDVEQEKVVVDEKEVQEVLDRILRDRAQLVPVEGKGPAVDGQIATVDFAAFENGEPV
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEEEEECCCCCCC
EGVKAESFDLALGERQALEDFEALVKTIPYGEEGEGEIHFPEDFIAKDLAGKTVTMKVKV
CCCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHCCCEEEEEEEE
HAIKERKLPALDDELAKSLGLESVEKLKEAITKSYTESRTGLNRSAAQKSLLDGLLKMVA
EEHHHCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FELPPSLVETQMNTLLADMAGRLERQGRSLESLGKSMEDLRKDMQPQADELARAQVLLLS
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VAKKEGLDVTDHEVTTQIYQLAMRTGEDFKALREQYERSGMIFVLRDRMLADKAMDLIYA
HHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
KANVKEVEPKAPETGAEADAAPGASASAQESK
CCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEYSAEDISPVRKKVVITTEPQEVEASIMGTIALYKTSVQVDGFRKGKVPASVIEQRFRD
CCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHH
KIYEEARQDLINVHINDVMQKLDVMPLAGLDVDTPDAFERGKGFAYTIEFEVLPTFDLPP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEEEEEEEEECCCCCC
YEGMDVEQEKVVVDEKEVQEVLDRILRDRAQLVPVEGKGPAVDGQIATVDFAAFENGEPV
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEEEEECCCCCCC
EGVKAESFDLALGERQALEDFEALVKTIPYGEEGEGEIHFPEDFIAKDLAGKTVTMKVKV
CCCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHCCCEEEEEEEE
HAIKERKLPALDDELAKSLGLESVEKLKEAITKSYTESRTGLNRSAAQKSLLDGLLKMVA
EEHHHCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FELPPSLVETQMNTLLADMAGRLERQGRSLESLGKSMEDLRKDMQPQADELARAQVLLLS
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VAKKEGLDVTDHEVTTQIYQLAMRTGEDFKALREQYERSGMIFVLRDRMLADKAMDLIYA
HHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
KANVKEVEPKAPETGAEADAAPGASASAQESK
CCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (omega=180)

Specific reaction: peptidylproline (omega=180) = peptidylproline (omega=0)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA