Click here to switch to the map view.

The map label for this gene is queF

Identifier: 220903928

GI number: 220903928

Start: 793446

End: 793949

Strand: Direct

Name: queF

Synonym: Ddes_0653

Alternate gene names: 220903928

Gene position: 793446-793949 (Clockwise)

Preceding gene: 220903923

Following gene: 220903929

Centisome position: 27.61

GC content: 57.14

Gene sequence:

>504_bases
ATGACCACTCGCAGCCAGGATCAGACCCGGGACCTCAAGGTTCTCGGCACAGGCCGCCTTACCTCCCCAGAAGGAGGCCC
CAGCGTAGCCTTGCTGGAGGCTTTTCCCAACTGTTTTCCGCAACGGCCCTATGTTATCAGCATCAGCTTTCCCGAATTTA
CATCGCTCTGCCCGGTGACCGGTCAGCCCGACTGCGGGACTATAACCGTTGAATACATTCCGGACGAACTGTGCGTGGAA
TCCAAAAGTTTCAAGCTGTATATGTTTGCTTTTCGCAATCATCAGTCTTTTATGGAGACCATCACCAACAACGTGCTGGA
AGACCTGCGAGCACTGCTCAATCCCTGCTGGTGCCGGGTCAAGGGCCTGTTCGCGCCGCGTGGCGGCACGCGCATCCATG
TTTTTGCCGAGGCCTTCAAGGACGGCATGCCCGAAGAACAGAGCGCTCTTGTGCGTGAAACCGTTCGTTCCTGGAAGTCC
GAACCGGACCCGCACCGGCCGTAA

Upstream 100 bases:

>100_bases
AATATTGTAGCCGCAAGCGCGATGGGTTACAAGTCCAATACGCGCCCGCGCCTGCCCGGTCCGGCCGGGCGCGGAGCATA
TTTTTCAAGGAGTTTAAGCT

Downstream 100 bases:

>100_bases
GCGCCGCCGCGCCGCCTGCTCTTGTCCGGCGGCGCGGGTTTGCCAGGACTTGCGCCGAAAAGCCCGGCCCAGGCCTGATA
GCCATGAGCAAAGACTACCG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MTTRSQDQTRDLKVLGTGRLTSPEGGPSVALLEAFPNCFPQRPYVISISFPEFTSLCPVTGQPDCGTITVEYIPDELCVE
SKSFKLYMFAFRNHQSFMETITNNVLEDLRALLNPCWCRVKGLFAPRGGTRIHVFAEAFKDGMPEEQSALVRETVRSWKS
EPDPHRP

Sequences:

>Translated_167_residues
MTTRSQDQTRDLKVLGTGRLTSPEGGPSVALLEAFPNCFPQRPYVISISFPEFTSLCPVTGQPDCGTITVEYIPDELCVE
SKSFKLYMFAFRNHQSFMETITNNVLEDLRALLNPCWCRVKGLFAPRGGTRIHVFAEAFKDGMPEEQSALVRETVRSWKS
EPDPHRP
>Mature_166_residues
TTRSQDQTRDLKVLGTGRLTSPEGGPSVALLEAFPNCFPQRPYVISISFPEFTSLCPVTGQPDCGTITVEYIPDELCVES
KSFKLYMFAFRNHQSFMETITNNVLEDLRALLNPCWCRVKGLFAPRGGTRIHVFAEAFKDGMPEEQSALVRETVRSWKSE
PDPHRP

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_DESDA (B8IYG6)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479240.1
- GeneID:   7284326
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0653
- HOGENOM:   HBG294103
- ProtClustDB:   PRK13258
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 18830; Mature: 18698

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRSQDQTRDLKVLGTGRLTSPEGGPSVALLEAFPNCFPQRPYVISISFPEFTSLCPVT
CCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
GQPDCGTITVEYIPDELCVESKSFKLYMFAFRNHQSFMETITNNVLEDLRALLNPCWCRV
CCCCCCEEEEEECCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGLFAPRGGTRIHVFAEAFKDGMPEEQSALVRETVRSWKSEPDPHRP
HCEECCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTRSQDQTRDLKVLGTGRLTSPEGGPSVALLEAFPNCFPQRPYVISISFPEFTSLCPVT
CCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
GQPDCGTITVEYIPDELCVESKSFKLYMFAFRNHQSFMETITNNVLEDLRALLNPCWCRV
CCCCCCEEEEEECCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGLFAPRGGTRIHVFAEAFKDGMPEEQSALVRETVRSWKSEPDPHRP
HCEECCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA