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The map label for this gene is punA [H]

Identifier: 220903739

GI number: 220903739

Start: 557374

End: 558285

Strand: Direct

Name: punA [H]

Synonym: Ddes_0461

Alternate gene names: 220903739

Gene position: 557374-558285 (Clockwise)

Preceding gene: 220903722

Following gene: 220903740

Centisome position: 19.4

GC content: 59.98

Gene sequence:

>912_bases
ATGCAAAATCCGCAAGAAGTCCAGTCGGCCGCGGCGGCTCTGCGCAAGGCTCTGGAGGCTGCGCCATGCAGCTTTTCACA
GGTTATGGGCATGCGAAAAATCACCGGCGCTACGGCAGAAGACAACAGTGTTCCCGTTGGTCTTGTGCTGGGTACGGGCC
TTTCGGGTCTGGCGGACCGGCTGGCGGAGCGGGTAGCCGTGCCTTATGCCGATCTGCCGGGTTTTCCCGTATCCAGCGTG
GATTCGCATGTGGGAGCCTTTGTGTGGGGGCGTTTTCCCTCATCCTGCGGCGAGGACGATGTACCGGGCCGTCCTGTGCT
TATCCAGCAGGGGCGCTGCCACCTTTATGAAGGCCGCAGCCCGGCCGAAGTGTGTATGGGGGTGCGCGTCATGGCGGCCA
TGGGCGTCAAGACGCTGGTGATCACCAACGCGGCGGGGGCGCTTAATCCCCATTTTGACGCCGGGTCCATCATGTGTATG
AGCGATTTTATCAACCATACGGGGTATTCGCCTCTGCTCGGCTGTAACTGCGAGCAATGGGGACCGCGTTTTCCCGACAT
GAGCGCGCCCTTTGATGCGGACCTGCGCACCCTTGCCATGGAAACTTCCGCCAGGCTGGGCCTGCGGCTGGAACGCGGCG
TGTACATTGGCGTGCGCGGCCCTGAAATGGAAACCCCTGCCGAAACGCGCATGTACCGGCAGTGGGGAGCCGATGCTGTG
GGCATGAGCACGGTGCTTGAGGTCATTGCGGCGCGCCATCTGGGTATGCGCGTGCTTGGCCTTTCATGTATTTCAAATAA
AAACTTGCCGGACTGTATGACCCCGGCGCCTTTGGAAGAAATTATTCTTGTGGCTGCCCAGGCGGGTAAAAATCTGGGCC
GCCTCATACGGGCAATGATGACAAAACTTTAA

Upstream 100 bases:

>100_bases
TGAAAATCCACACGCAGTACTGTGGGCGGATGAAAAAAGTCGCCCCCGGTCATTGCGCGGCATTACCCGCTGCGGCACTG
TTAAAATCAGGAGGGATAAT

Downstream 100 bases:

>100_bases
AAAATACGTACAAGCGCAGCCCGGACTCATTTTTTTACACTAAAAGGCAAAGCGCCCATGCATTATAACGATTCTCTTCG
CAACGTTGCTATTATCGCCC

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MQNPQEVQSAAAALRKALEAAPCSFSQVMGMRKITGATAEDNSVPVGLVLGTGLSGLADRLAERVAVPYADLPGFPVSSV
DSHVGAFVWGRFPSSCGEDDVPGRPVLIQQGRCHLYEGRSPAEVCMGVRVMAAMGVKTLVITNAAGALNPHFDAGSIMCM
SDFINHTGYSPLLGCNCEQWGPRFPDMSAPFDADLRTLAMETSARLGLRLERGVYIGVRGPEMETPAETRMYRQWGADAV
GMSTVLEVIAARHLGMRVLGLSCISNKNLPDCMTPAPLEEIILVAAQAGKNLGRLIRAMMTKL

Sequences:

>Translated_303_residues
MQNPQEVQSAAAALRKALEAAPCSFSQVMGMRKITGATAEDNSVPVGLVLGTGLSGLADRLAERVAVPYADLPGFPVSSV
DSHVGAFVWGRFPSSCGEDDVPGRPVLIQQGRCHLYEGRSPAEVCMGVRVMAAMGVKTLVITNAAGALNPHFDAGSIMCM
SDFINHTGYSPLLGCNCEQWGPRFPDMSAPFDADLRTLAMETSARLGLRLERGVYIGVRGPEMETPAETRMYRQWGADAV
GMSTVLEVIAARHLGMRVLGLSCISNKNLPDCMTPAPLEEIILVAAQAGKNLGRLIRAMMTKL
>Mature_303_residues
MQNPQEVQSAAAALRKALEAAPCSFSQVMGMRKITGATAEDNSVPVGLVLGTGLSGLADRLAERVAVPYADLPGFPVSSV
DSHVGAFVWGRFPSSCGEDDVPGRPVLIQQGRCHLYEGRSPAEVCMGVRVMAAMGVKTLVITNAAGALNPHFDAGSIMCM
SDFINHTGYSPLLGCNCEQWGPRFPDMSAPFDADLRTLAMETSARLGLRLERGVYIGVRGPEMETPAETRMYRQWGADAV
GMSTVLEVIAARHLGMRVLGLSCISNKNLPDCMTPAPLEEIILVAAQAGKNLGRLIRAMMTKL

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=265, Percent_Identity=42.2641509433962, Blast_Score=198, Evalue=4e-51,
Organism=Homo sapiens, GI47132622, Length=225, Percent_Identity=28.4444444444444, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1788746, Length=226, Percent_Identity=39.3805309734513, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17541190, Length=264, Percent_Identity=35.9848484848485, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6323238, Length=279, Percent_Identity=38.3512544802867, Blast_Score=170, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24656090, Length=271, Percent_Identity=39.8523985239852, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24656093, Length=271, Percent_Identity=39.8523985239852, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI45552887, Length=227, Percent_Identity=42.7312775330396, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI45552885, Length=227, Percent_Identity=42.7312775330396, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24762376, Length=224, Percent_Identity=35.7142857142857, Blast_Score=111, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 32321; Mature: 32321

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNPQEVQSAAAALRKALEAAPCSFSQVMGMRKITGATAEDNSVPVGLVLGTGLSGLADR
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHH
LAERVAVPYADLPGFPVSSVDSHVGAFVWGRFPSSCGEDDVPGRPVLIQQGRCHLYEGRS
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEECCCC
PAEVCMGVRVMAAMGVKTLVITNAAGALNPHFDAGSIMCMSDFINHTGYSPLLGCNCEQW
HHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHH
GPRFPDMSAPFDADLRTLAMETSARLGLRLERGVYIGVRGPEMETPAETRMYRQWGADAV
CCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCEEEEECCCCCCCCHHHHHHHHHCCCHH
GMSTVLEVIAARHLGMRVLGLSCISNKNLPDCMTPAPLEEIILVAAQAGKNLGRLIRAMM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
TKL
HCC
>Mature Secondary Structure
MQNPQEVQSAAAALRKALEAAPCSFSQVMGMRKITGATAEDNSVPVGLVLGTGLSGLADR
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHH
LAERVAVPYADLPGFPVSSVDSHVGAFVWGRFPSSCGEDDVPGRPVLIQQGRCHLYEGRS
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEECCCC
PAEVCMGVRVMAAMGVKTLVITNAAGALNPHFDAGSIMCMSDFINHTGYSPLLGCNCEQW
HHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHH
GPRFPDMSAPFDADLRTLAMETSARLGLRLERGVYIGVRGPEMETPAETRMYRQWGADAV
CCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCEEEEECCCCCCCCHHHHHHHHHCCCHH
GMSTVLEVIAARHLGMRVLGLSCISNKNLPDCMTPAPLEEIILVAAQAGKNLGRLIRAMM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
TKL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]