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The map label for this gene is bioF

Identifier: 220903701

GI number: 220903701

Start: 514902

End: 516056

Strand: Reverse

Name: bioF

Synonym: Ddes_0423

Alternate gene names: 220903701

Gene position: 516056-514902 (Counterclockwise)

Preceding gene: 220903702

Following gene: 220903699

Centisome position: 17.96

GC content: 62.16

Gene sequence:

>1155_bases
GTGAGCGACGTTGCCGCCAGGCTCGAAACCATCAGGGCCGCGCACCTGTACCGGCGCCTGCGCACGCTTTCCACACCGCA
GGACAGGGAAGTCGTCATCGACGGGCGACGCGTGCTGCTCTTTTCTTCCAACAGTTATCTGGGCCTCGGCATCAACGCGC
ATATCCGCCGCAGCGCGGTGCGCGCACTGGAAAAATTTGGCACCGGGGCTGGCGGGTCGCGCCTGGTTACGGGCAACATG
ACGCCGCACATGCAGCTTGAGTCTGCCCTGGCGGCATTCAAAGGCAGCGAATCCGCCCTGGCCTTTACCAGCGGCTATAC
GGCCAATATGGGCGTTATCAGCGCCCTGTGCCACAAGGATACGGTCATCTTCAGCGATGCGCTCAACCATGCCAGCATCA
TTGACGGCTGCCGCCTGGCGCGGGGGCGTACCGTTGTTTACGCCCACAACGATATGGACGACCTGCTGGAAAAAATCCGG
CAACTGCGCCCCAGGCAGGGCTTTATCATCACCGACGGCGTATTCAGCATGGACGGTGATCTGGCACGGCTGCCGGAACT
GGCCCACATTGCCCGCAGTTACGGCCTGACACTTATGGTGGATGATGCCCATGCCACAGGCGTGCTAGGCGCTACGGGAC
GAGGTACGCTGGAACACTTCGGTCTCAGCCATGAGGATGTTCCGGTTGTCACGGGCACACTCAGCAAGGCCATTCCCAGT
GAAGGCGGTTTTGTCTGCGGCAGTGAGATCCTTTGCGAACTGCTCAGGAATACGGCGCGGCCCTTTATTTTTACCACTGC
TCCTTCACCTGCCGCCGTTGCCGCCGCCACTGCGGGCATCGGGCATATTGCCGCGCACCCCGGACTGGTGCGCCGCCTTC
AGGACAACGTGGCCTATTTTACCGGCAGCCTGGCTGAACAGGGTATGCACGTACCGGGGCAAAGCCCCATCATCCCCATC
ATGGCCGGTGAAGAAGAAAAAGCCGTGCGCGCTGCGGAAGCCCTGCTGGCTCTGGGGGTATTTGCCCCCTGCATCCGTTA
TCCCACAGTGCCGAGAGGGCAGGCGCGGCTGCGGCTGACTGTCATGGCCTCCCACACCCGTGACGACCTGGATCATGCGG
CGGTCAGCCTGCGCAAGGCCTTGGCAGGCGTTTAA

Upstream 100 bases:

>100_bases
TGGGCGGGCGCATTTTTCTTTTCAGGGGCCAGCAGGCCGAGATCGGACAGTGTGTGGACTACCTTGAAAACCAGCCGGTC
ATGGTTGTTATGGAGTCTCT

Downstream 100 bases:

>100_bases
AAAATCCTGTTTCCGGCGTCAGCGGCGTGCGCCGCCGAACCATCGGCGCTTCAGGCACACTTTGCCCGAAGATCAGGCCT
GATGCATTTCACTGCAAACA

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSDVAARLETIRAAHLYRRLRTLSTPQDREVVIDGRRVLLFSSNSYLGLGINAHIRRSAVRALEKFGTGAGGSRLVTGNM
TPHMQLESALAAFKGSESALAFTSGYTANMGVISALCHKDTVIFSDALNHASIIDGCRLARGRTVVYAHNDMDDLLEKIR
QLRPRQGFIITDGVFSMDGDLARLPELAHIARSYGLTLMVDDAHATGVLGATGRGTLEHFGLSHEDVPVVTGTLSKAIPS
EGGFVCGSEILCELLRNTARPFIFTTAPSPAAVAAATAGIGHIAAHPGLVRRLQDNVAYFTGSLAEQGMHVPGQSPIIPI
MAGEEEKAVRAAEALLALGVFAPCIRYPTVPRGQARLRLTVMASHTRDDLDHAAVSLRKALAGV

Sequences:

>Translated_384_residues
MSDVAARLETIRAAHLYRRLRTLSTPQDREVVIDGRRVLLFSSNSYLGLGINAHIRRSAVRALEKFGTGAGGSRLVTGNM
TPHMQLESALAAFKGSESALAFTSGYTANMGVISALCHKDTVIFSDALNHASIIDGCRLARGRTVVYAHNDMDDLLEKIR
QLRPRQGFIITDGVFSMDGDLARLPELAHIARSYGLTLMVDDAHATGVLGATGRGTLEHFGLSHEDVPVVTGTLSKAIPS
EGGFVCGSEILCELLRNTARPFIFTTAPSPAAVAAATAGIGHIAAHPGLVRRLQDNVAYFTGSLAEQGMHVPGQSPIIPI
MAGEEEKAVRAAEALLALGVFAPCIRYPTVPRGQARLRLTVMASHTRDDLDHAAVSLRKALAGV
>Mature_383_residues
SDVAARLETIRAAHLYRRLRTLSTPQDREVVIDGRRVLLFSSNSYLGLGINAHIRRSAVRALEKFGTGAGGSRLVTGNMT
PHMQLESALAAFKGSESALAFTSGYTANMGVISALCHKDTVIFSDALNHASIIDGCRLARGRTVVYAHNDMDDLLEKIRQ
LRPRQGFIITDGVFSMDGDLARLPELAHIARSYGLTLMVDDAHATGVLGATGRGTLEHFGLSHEDVPVVTGTLSKAIPSE
GGFVCGSEILCELLRNTARPFIFTTAPSPAAVAAATAGIGHIAAHPGLVRRLQDNVAYFTGSLAEQGMHVPGQSPIIPIM
AGEEEKAVRAAEALLALGVFAPCIRYPTVPRGQARLRLTVMASHTRDDLDHAAVSLRKALAGV

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI7657118, Length=369, Percent_Identity=36.8563685636856, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI284448556, Length=395, Percent_Identity=34.4303797468354, Blast_Score=232, Evalue=5e-61,
Organism=Homo sapiens, GI4758668, Length=356, Percent_Identity=33.1460674157303, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI83977440, Length=370, Percent_Identity=32.7027027027027, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI83977442, Length=370, Percent_Identity=32.7027027027027, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI83977444, Length=370, Percent_Identity=32.7027027027027, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI119220554, Length=355, Percent_Identity=30.1408450704225, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI4502025, Length=383, Percent_Identity=30.2872062663185, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI40316939, Length=383, Percent_Identity=30.2872062663185, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI5454084, Length=389, Percent_Identity=25.706940874036, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI1790046, Length=372, Percent_Identity=36.5591397849462, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1786993, Length=352, Percent_Identity=36.6477272727273, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71994529, Length=373, Percent_Identity=34.0482573726542, Blast_Score=228, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI32566772, Length=355, Percent_Identity=31.5492957746479, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17560914, Length=353, Percent_Identity=28.0453257790368, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17560912, Length=353, Percent_Identity=28.0453257790368, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71982617, Length=297, Percent_Identity=28.956228956229, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71982625, Length=278, Percent_Identity=29.4964028776978, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320438, Length=359, Percent_Identity=34.8189415041783, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6320267, Length=352, Percent_Identity=30.6818181818182, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323954, Length=270, Percent_Identity=29.6296296296296, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24662918, Length=374, Percent_Identity=35.2941176470588, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI17137420, Length=356, Percent_Identity=34.5505617977528, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI17136286, Length=408, Percent_Identity=27.4509803921569, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24653280, Length=285, Percent_Identity=27.719298245614, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24653276, Length=285, Percent_Identity=27.719298245614, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24653278, Length=285, Percent_Identity=27.719298245614, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_DESDA (B8J3V0)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479013.1
- ProteinModelPortal:   B8J3V0
- GeneID:   7284087
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0423
- HOGENOM:   HBG649839
- ProtClustDB:   CLSK2546350
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 40917; Mature: 40785

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 18-18 BINDING 130-130 BINDING 176-176 BINDING 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVAARLETIRAAHLYRRLRTLSTPQDREVVIDGRRVLLFSSNSYLGLGINAHIRRSAV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCEEEECCCHHHHHHHH
RALEKFGTGAGGSRLVTGNMTPHMQLESALAAFKGSESALAFTSGYTANMGVISALCHKD
HHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCC
TVIFSDALNHASIIDGCRLARGRTVVYAHNDMDDLLEKIRQLRPRQGFIITDGVFSMDGD
CEEEHHHCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEECCCCCCCCC
LARLPELAHIARSYGLTLMVDDAHATGVLGATGRGTLEHFGLSHEDVPVVTGTLSKAIPS
HHHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCHHHHCCCCCCCCCEEECHHHHHCCC
EGGFVCGSEILCELLRNTARPFIFTTAPSPAAVAAATAGIGHIAAHPGLVRRLQDNVAYF
CCCEEECHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCHHHHCCHHHHHHHHHHHHHH
TGSLAEQGMHVPGQSPIIPIMAGEEEKAVRAAEALLALGVFAPCIRYPTVPRGQARLRLT
CCHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
VMASHTRDDLDHAAVSLRKALAGV
EEECCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDVAARLETIRAAHLYRRLRTLSTPQDREVVIDGRRVLLFSSNSYLGLGINAHIRRSAV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCEEEECCCHHHHHHHH
RALEKFGTGAGGSRLVTGNMTPHMQLESALAAFKGSESALAFTSGYTANMGVISALCHKD
HHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCC
TVIFSDALNHASIIDGCRLARGRTVVYAHNDMDDLLEKIRQLRPRQGFIITDGVFSMDGD
CEEEHHHCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEECCCCCCCCC
LARLPELAHIARSYGLTLMVDDAHATGVLGATGRGTLEHFGLSHEDVPVVTGTLSKAIPS
HHHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCHHHHCCCCCCCCCEEECHHHHHCCC
EGGFVCGSEILCELLRNTARPFIFTTAPSPAAVAAATAGIGHIAAHPGLVRRLQDNVAYF
CCCEEECHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCHHHHCCHHHHHHHHHHHHHH
TGSLAEQGMHVPGQSPIIPIMAGEEEKAVRAAEALLALGVFAPCIRYPTVPRGQARLRLT
CCHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
VMASHTRDDLDHAAVSLRKALAGV
EEECCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA