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The map label for this gene is tsf

Identifier: 220903627

GI number: 220903627

Start: 422978

End: 423838

Strand: Direct

Name: tsf

Synonym: Ddes_0347

Alternate gene names: 220903627

Gene position: 422978-423838 (Clockwise)

Preceding gene: 220903626

Following gene: 220903628

Centisome position: 14.72

GC content: 58.65

Gene sequence:

>861_bases
ATGGCTGCTATCACTGCTCAGATGGTGAAAGAACTGCGCGAAATGACCGGCGCGGGCATGATGGACTGCAAGAAAGCCCT
GGTGGAAGTAGAAGGCGACCTGGAAAAAGCTGTTGACTGGCTGCGCCAGAAGGGCATGGCCAAGGCTGCCAAAAAGTCCG
GCCGCGCCACCAGCGAAGGTCTTGTAACCGTGGCTCTGAGCGATGACGGCAAGACCGTGGCCATGGCTTCGCTGCTGTGC
GAAACCGACTTTGTGGCCCGCGGCGACCAGTTTCAGGACATGGCCGCCAAGGTGGCCAAAAGCGTGCTGGACAACGCCCC
CGCCGACGCCGCTGCCCTTGAGGCCCTTATGGGTGAAGAAGTGACCCAGCTCATCGCCTCTGTGGGCGAAAACATGCAGC
TCGGCAGGTTTGCCCGTCACGTCAAACCCTGCGAAAGCTCCCTGGTGGGCCAGTACATCCACGCCAACGGCAAGATCGGT
GTGCTGGTTTTCCTGACCTGCGGCAAGGCTGAAAGTGTCGACAAGCCCGAAGTGCAGGAGCTTGCCAAAAATATCGCCAT
GCAGGTGGCTGCTGCCAGCCCCATGGCTCTTGATGCCGCAAGCCTTGACCAGGCCGCTGTGGAACGCGAGCGCGAAGTGT
ATCGCCAGAAGGCTCTGGAAGAAGGCAAACCCGCCAATATCGTTGACAAGATTGCTGACGGCGCGGTGAAAAAGTTCCAG
AAAGAAGTGTGCCTTATGGAACAGCCCTACATCCGCGATGACAAGAAGACCATTACCGATGTCGTGCGTGAAACGGGCAA
GGCCGTGGGCGATGAAATCACTGTTACCGGCTTTGAACGCATCCAGCTCGCTGCCGAGTAA

Upstream 100 bases:

>100_bases
CTGATTTATGGCTGCGCTGCCCCGTTTTGGATGCACCTCTGCAATTCAGGAAGTAATTTTGATAACTCCGGCTGCAGGCC
GGACGCATTTGGAGAATACA

Downstream 100 bases:

>100_bases
TGACATCTGACTTGTCCGAGCCGGAGCAGCCTGCTGGTCCGGCCATATGCCGGGCGTTTTTGAGCGCATGCTTGCAACGG
CATTCCGGTTTTCCGCTTTC

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MAAITAQMVKELREMTGAGMMDCKKALVEVEGDLEKAVDWLRQKGMAKAAKKSGRATSEGLVTVALSDDGKTVAMASLLC
ETDFVARGDQFQDMAAKVAKSVLDNAPADAAALEALMGEEVTQLIASVGENMQLGRFARHVKPCESSLVGQYIHANGKIG
VLVFLTCGKAESVDKPEVQELAKNIAMQVAAASPMALDAASLDQAAVEREREVYRQKALEEGKPANIVDKIADGAVKKFQ
KEVCLMEQPYIRDDKKTITDVVRETGKAVGDEITVTGFERIQLAAE

Sequences:

>Translated_286_residues
MAAITAQMVKELREMTGAGMMDCKKALVEVEGDLEKAVDWLRQKGMAKAAKKSGRATSEGLVTVALSDDGKTVAMASLLC
ETDFVARGDQFQDMAAKVAKSVLDNAPADAAALEALMGEEVTQLIASVGENMQLGRFARHVKPCESSLVGQYIHANGKIG
VLVFLTCGKAESVDKPEVQELAKNIAMQVAAASPMALDAASLDQAAVEREREVYRQKALEEGKPANIVDKIADGAVKKFQ
KEVCLMEQPYIRDDKKTITDVVRETGKAVGDEITVTGFERIQLAAE
>Mature_285_residues
AAITAQMVKELREMTGAGMMDCKKALVEVEGDLEKAVDWLRQKGMAKAAKKSGRATSEGLVTVALSDDGKTVAMASLLCE
TDFVARGDQFQDMAAKVAKSVLDNAPADAAALEALMGEEVTQLIASVGENMQLGRFARHVKPCESSLVGQYIHANGKIGV
LVFLTCGKAESVDKPEVQELAKNIAMQVAAASPMALDAASLDQAAVEREREVYRQKALEEGKPANIVDKIADGAVKKFQK
EVCLMEQPYIRDDKKTITDVVRETGKAVGDEITVTGFERIQLAAE

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=309, Percent_Identity=27.831715210356, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI291084500, Length=330, Percent_Identity=26.969696969697, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI291084502, Length=102, Percent_Identity=40.1960784313725, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI291084498, Length=102, Percent_Identity=40.1960784313725, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786366, Length=283, Percent_Identity=38.86925795053, Blast_Score=175, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17561440, Length=310, Percent_Identity=26.4516129032258, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19921466, Length=290, Percent_Identity=28.2758620689655, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_DESDA (B8J3M6)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002478939.1
- GeneID:   7284004
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0347
- HOGENOM:   HBG713289
- ProtClustDB:   PRK09377
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 30693; Mature: 30561

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAITAQMVKELREMTGAGMMDCKKALVEVEGDLEKAVDWLRQKGMAKAAKKSGRATSEG
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
LVTVALSDDGKTVAMASLLCETDFVARGDQFQDMAAKVAKSVLDNAPADAAALEALMGEE
EEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VTQLIASVGENMQLGRFARHVKPCESSLVGQYIHANGKIGVLVFLTCGKAESVDKPEVQE
HHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHH
LAKNIAMQVAAASPMALDAASLDQAAVEREREVYRQKALEEGKPANIVDKIADGAVKKFQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KEVCLMEQPYIRDDKKTITDVVRETGKAVGDEITVTGFERIQLAAE
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECHHHHHEECC
>Mature Secondary Structure 
AAITAQMVKELREMTGAGMMDCKKALVEVEGDLEKAVDWLRQKGMAKAAKKSGRATSEG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
LVTVALSDDGKTVAMASLLCETDFVARGDQFQDMAAKVAKSVLDNAPADAAALEALMGEE
EEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VTQLIASVGENMQLGRFARHVKPCESSLVGQYIHANGKIGVLVFLTCGKAESVDKPEVQE
HHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHH
LAKNIAMQVAAASPMALDAASLDQAAVEREREVYRQKALEEGKPANIVDKIADGAVKKFQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KEVCLMEQPYIRDDKKTITDVVRETGKAVGDEITVTGFERIQLAAE
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA