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The map label for this gene is thiE [H]

Identifier: 220903491

GI number: 220903491

Start: 260281

End: 260916

Strand: Direct

Name: thiE [H]

Synonym: Ddes_0209

Alternate gene names: 220903491

Gene position: 260281-260916 (Clockwise)

Preceding gene: 220903490

Following gene: 220903493

Centisome position: 9.06

GC content: 60.69

Gene sequence:

>636_bases
ATGCCAAGAATTCTTCCCGGTGAAACAGATATTTATGCCATTACTGATTCCCGCCTGTCGCTGGGGCGCTCCCTTGAAGA
GGTGGCTTCAGCCCTGCTGGGGGCGGGCGTGCGTCTTTTGCAGTACCGCGAAAAAAATTTCAAAGGCGGTGAAATGCTTG
AAGAATGCCGCCTGCTGCGCCGCCTGACCCGTGAGGCGGGGGCCTGCTTTATTGTTAACGATCATGTGGATATTGCCATG
CTTGTGGATGCTGACGGCGTGCATGTGGGGCAGGAGGACCTGCCTGTGCCTGAAGTGCGCAGGCTTGTGGGACCGGACAT
GATCATCGGCCTTTCCACCCACGAGCCGGATCAGGCGCTGGCCGCGCGGGCGCTGGGGGCGGACTATCTGGGTGTTGGCC
CCATTTTCGCCACGCAGACCAAGGAAGATGTGGTGGCCCCCGTGGGTTTTGGTTATCTGGATTGGGTTGCCGCCAACATT
GATATGCCTTTTGTAGCCATAGGCGGCATCAAGGCCCACAATATCGCTGAAGTGGCCCGCCACGGCGCGCGCTGTTGCAG
CCTGGTTTCCGAACTGGTGGGCGCGCCGGACATTGCCGCGCGTGTGGATGCCGTGCGGCGGGCCATGCGCGGCTAG

Upstream 100 bases:

>100_bases
TGCCGCAGCGTCTGTCCCACACGTCTTTTCATCCCGCGCGTTTTGTACAGAGTTGCCTGCGCCGCGCCATCGCGGCCATA
AAAAAATAAGGAAGCAGCAA

Downstream 100 bases:

>100_bases
AGCGTTTTGAGTTCTTGCTGCTCTGACGGCGGCGCAGGCGCTTGCTGCTGCGGCATAAAAGCATGCTTGCCCGCCCGGTC
CCGGTTCACTGGGCGGGCCG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MPRILPGETDIYAITDSRLSLGRSLEEVASALLGAGVRLLQYREKNFKGGEMLEECRLLRRLTREAGACFIVNDHVDIAM
LVDADGVHVGQEDLPVPEVRRLVGPDMIIGLSTHEPDQALAARALGADYLGVGPIFATQTKEDVVAPVGFGYLDWVAANI
DMPFVAIGGIKAHNIAEVARHGARCCSLVSELVGAPDIAARVDAVRRAMRG

Sequences:

>Translated_211_residues
MPRILPGETDIYAITDSRLSLGRSLEEVASALLGAGVRLLQYREKNFKGGEMLEECRLLRRLTREAGACFIVNDHVDIAM
LVDADGVHVGQEDLPVPEVRRLVGPDMIIGLSTHEPDQALAARALGADYLGVGPIFATQTKEDVVAPVGFGYLDWVAANI
DMPFVAIGGIKAHNIAEVARHGARCCSLVSELVGAPDIAARVDAVRRAMRG
>Mature_210_residues
PRILPGETDIYAITDSRLSLGRSLEEVASALLGAGVRLLQYREKNFKGGEMLEECRLLRRLTREAGACFIVNDHVDIAML
VDADGVHVGQEDLPVPEVRRLVGPDMIIGLSTHEPDQALAARALGADYLGVGPIFATQTKEDVVAPVGFGYLDWVAANID
MPFVAIGGIKAHNIAEVARHGARCCSLVSELVGAPDIAARVDAVRRAMRG

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=197, Percent_Identity=34.5177664974619, Blast_Score=81, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=221, Percent_Identity=35.2941176470588, Blast_Score=114, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22652; Mature: 22521

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRILPGETDIYAITDSRLSLGRSLEEVASALLGAGVRLLQYREKNFKGGEMLEECRLLR
CCCCCCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RLTREAGACFIVNDHVDIAMLVDADGVHVGQEDLPVPEVRRLVGPDMIIGLSTHEPDQAL
HHHHHCCEEEEEECCCCEEEEEECCCCEECCCCCCHHHHHHHCCCCEEEECCCCCCHHHH
AARALGADYLGVGPIFATQTKEDVVAPVGFGYLDWVAANIDMPFVAIGGIKAHNIAEVAR
HHHHHCCCCCCCCCEEECCCHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
HGARCCSLVSELVGAPDIAARVDAVRRAMRG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PRILPGETDIYAITDSRLSLGRSLEEVASALLGAGVRLLQYREKNFKGGEMLEECRLLR
CCCCCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RLTREAGACFIVNDHVDIAMLVDADGVHVGQEDLPVPEVRRLVGPDMIIGLSTHEPDQAL
HHHHHCCEEEEEECCCCEEEEEECCCCEECCCCCCHHHHHHHCCCCEEEECCCCCCHHHH
AARALGADYLGVGPIFATQTKEDVVAPVGFGYLDWVAANIDMPFVAIGGIKAHNIAEVAR
HHHHHCCCCCCCCCEEECCCHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
HGARCCSLVSELVGAPDIAARVDAVRRAMRG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA