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The map label for this gene is nth [H]

Identifier: 220903480

GI number: 220903480

Start: 249832

End: 250518

Strand: Reverse

Name: nth [H]

Synonym: Ddes_0198

Alternate gene names: 220903480

Gene position: 250518-249832 (Counterclockwise)

Preceding gene: 220903486

Following gene: 220903479

Centisome position: 8.72

GC content: 64.48

Gene sequence:

>687_bases
ATGAGCGCAAAAAATTCCCGCCCGGCACGGGCGCAAAAAGTTCTGGCAGCCCTGCGCACCCGCTACCCCGCCCCCCATAC
CCATCTGGACGCCGAAACGGCGTGGCAGCTGCTGGTGGCTACGGTGCTGGCGGCCCAGTGTACGGACGCCCGGGTCAACA
CCGTCACTCCGGAACTGTTCCGCCGCTGGCCCGGCCCCGCCGACCTCATGGGGGTTCCGGTTGAGGAACTGGAGGCGGTC
ATACGCTCCACGGGTTTTTACCGCAGCAAGGCCAAAAACCTGCTGGGGGCCGCAGCCCGCGTATGCGAGGTATACGACGG
GCGGATCCCCAACAGCCTTGAAGAACTCATCACCCTGCCCGGTGTGGCCCGCAAGACGGCCAATGTGGTACTGTTCGGCG
CTTTCGGCATCAATGAGGGTCTCGCGGTAGACACACACGTCAAGCGCATCAGCTACAGGCTCGGGCTGACCGAATCAACG
GACCCCGTTGTTATCGAGCGCGACCTTATGGCCCTTTTCCCCCGTGAAGAATGGGGTGATGTGAACCACCGCATGGTCTG
GTTCGGGCGTGAGGTATGCGAAGCGCGCAAGCCCCTGTGCGGCCAGTGCGAAATGGCGATCTTCTGCCCCAGGCTGGAGC
CGCCCAAAACACCGCGCCCGGCCCGACGCAAGGTTCCGCCGGCCTAG

Upstream 100 bases:

>100_bases
TTGCGAGGCAGCTTATACCCAAACCCGCAAAAAACAAAGAGGCCGCGAACCGCGCCCGCCACGGCTTGCAGGCTGCCGAC
GCCTGTGGCATAGGACGGAC

Downstream 100 bases:

>100_bases
CCCGCAGGCCTGCCGCAAGCGGTACCGCGACCTGCATGCGCCGGATGCAGTGAGTTGCAGGCAGCGGCCCGCAACACGGC
CATCGCCTCAAAGATACACG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSAKNSRPARAQKVLAALRTRYPAPHTHLDAETAWQLLVATVLAAQCTDARVNTVTPELFRRWPGPADLMGVPVEELEAV
IRSTGFYRSKAKNLLGAAARVCEVYDGRIPNSLEELITLPGVARKTANVVLFGAFGINEGLAVDTHVKRISYRLGLTEST
DPVVIERDLMALFPREEWGDVNHRMVWFGREVCEARKPLCGQCEMAIFCPRLEPPKTPRPARRKVPPA

Sequences:

>Translated_228_residues
MSAKNSRPARAQKVLAALRTRYPAPHTHLDAETAWQLLVATVLAAQCTDARVNTVTPELFRRWPGPADLMGVPVEELEAV
IRSTGFYRSKAKNLLGAAARVCEVYDGRIPNSLEELITLPGVARKTANVVLFGAFGINEGLAVDTHVKRISYRLGLTEST
DPVVIERDLMALFPREEWGDVNHRMVWFGREVCEARKPLCGQCEMAIFCPRLEPPKTPRPARRKVPPA
>Mature_227_residues
SAKNSRPARAQKVLAALRTRYPAPHTHLDAETAWQLLVATVLAAQCTDARVNTVTPELFRRWPGPADLMGVPVEELEAVI
RSTGFYRSKAKNLLGAAARVCEVYDGRIPNSLEELITLPGVARKTANVVLFGAFGINEGLAVDTHVKRISYRLGLTESTD
PVVIERDLMALFPREEWGDVNHRMVWFGREVCEARKPLCGQCEMAIFCPRLEPPKTPRPARRKVPPA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=207, Percent_Identity=31.8840579710145, Blast_Score=100, Evalue=8e-22,
Organism=Homo sapiens, GI6912520, Length=182, Percent_Identity=29.1208791208791, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI190358497, Length=182, Percent_Identity=29.1208791208791, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI115298654, Length=182, Percent_Identity=29.1208791208791, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI115298652, Length=182, Percent_Identity=29.1208791208791, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI115298648, Length=182, Percent_Identity=29.1208791208791, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI115298650, Length=182, Percent_Identity=29.1208791208791, Blast_Score=71, Evalue=6e-13,
Organism=Escherichia coli, GI1787920, Length=201, Percent_Identity=36.81592039801, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789331, Length=196, Percent_Identity=26.530612244898, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=176, Percent_Identity=36.9318181818182, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6324530, Length=183, Percent_Identity=26.2295081967213, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319304, Length=149, Percent_Identity=28.8590604026846, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550361, Length=184, Percent_Identity=33.695652173913, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25337; Mature: 25205

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKNSRPARAQKVLAALRTRYPAPHTHLDAETAWQLLVATVLAAQCTDARVNTVTPELF
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RRWPGPADLMGVPVEELEAVIRSTGFYRSKAKNLLGAAARVCEVYDGRIPNSLEELITLP
HHCCCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
GVARKTANVVLFGAFGINEGLAVDTHVKRISYRLGLTESTDPVVIERDLMALFPREEWGD
CCHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHCCCHHHCC
VNHRMVWFGREVCEARKPLCGQCEMAIFCPRLEPPKTPRPARRKVPPA
CCCEEEEHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAKNSRPARAQKVLAALRTRYPAPHTHLDAETAWQLLVATVLAAQCTDARVNTVTPELF
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RRWPGPADLMGVPVEELEAVIRSTGFYRSKAKNLLGAAARVCEVYDGRIPNSLEELITLP
HHCCCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
GVARKTANVVLFGAFGINEGLAVDTHVKRISYRLGLTESTDPVVIERDLMALFPREEWGD
CCHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHCCCHHHCC
VNHRMVWFGREVCEARKPLCGQCEMAIFCPRLEPPKTPRPARRKVPPA
CCCEEEEHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]