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The map label for this gene is gltX [H]

Identifier: 220903476

GI number: 220903476

Start: 243345

End: 244736

Strand: Direct

Name: gltX [H]

Synonym: Ddes_0192

Alternate gene names: 220903476

Gene position: 243345-244736 (Clockwise)

Preceding gene: 220903475

Following gene: 220903481

Centisome position: 8.47

GC content: 60.78

Gene sequence:

>1392_bases
ATGACGCAAGTGGTTACACGATTCGCCCCAAGCCCCACGGGACACCTGCATATAGGCGGTGCGCGCACGGCCATTTTCTG
CTGGCTTCTGGCCCGTCACTTCAACGGCCGCTTTCATCTGCGCATAGAAGACACCGATCTTTTGCGCTCAAAGCAGGAGT
ATACCGACTCCATCCTGGCTTCCATGCGCTGGCTCGGGCTGGACTGGGACGGTGAGCTGACCTATCAGACGCAGCGTACC
GACATCTACAACGCCTATGTGGATAAACTGCTGGAAAACGGCCACGCCTACTGGTGCTCCTGCACCCCCGAGACAGTGGA
GGCCATGCGTGAAGAAGCCCGCGCGAAGGGACTCAAGCCGCGCTATAACGGCTGTTGCCGCAACCGCGACCTGGGACCGG
GCGAAGGGCATTGCGTGCGCCTCAAGGCGCCGCTGTCGGGCAAGGTTGTTTTTGACGACCTGGTCAAGGGCAAGATTGCC
GTGGATGTGGGCGAACTGGACGACATGGTCATCCGCCGCGCTGACGGCATGCCCACGTATAATATGGCCGTGGTGGTGGA
CGATCATGACATGGGCATAACCCACGTCATCCGTGGCGATGATCATGTATCCAATACGCCGCGCCAGATTCTTATTTATG
AGGCTCTGGGCCTGTCCGTGCCGCGCTTCGGCCATGTACCGATGATTCTTGGTCCTGACCGCCAGAAGCTTTCAAAGCGC
CACGGCGCGCGCGCCGTTATCGAATACCAGCAGGATGGCCTGCTGCCGCAGGCCCTTGTGAACTACCTTGTGCGTCTGGG
CTGGTCCCACGGCAATCAGGAGCTGTTCACCCCGGAAGAACTGGTGGAATACTTCGACGGCACCAACCTCAATCCTGCGG
CCGCAGCCTTTGACCCGACCAAGCTCGAATGGTGCAATGCCCACTTTATGCGCGAAATGCCCCTGGCGGAGCTGGCCGCG
CTTACTGCGCCCTTTGTGGAGCAGGCGGGCCTTGGCAGCCTGCCGCAGGAACGCCTGGAACCGTTGTGCGCCATGTTCCG
TGAACGCGCCAACAACCTCAAGGCCCTGGCCGAAAGCTTCCGCCCCCTGCTTGTTCCTGCGGCGGAGCTTGAATACGCCG
AAAAGGACGCCGCCAAACACCTGGCGGAAGCAGGCAAGGCTCATCTGACTGCCCTGGCTGCCGTATTTGCCGCCTGTGAT
CCCTTTACGGCCGAAAACCTTGAGGCTGCCCTTAACGCCTATGTGGCGGACAACGGCCTCAAGTTCAAGGAGGTGGCCCC
GCCTTTGCGCACTGCCCTTATGGGCTTTATGGGCGGCTCGCACCTGAACGAGATCATGGCCTTTCTGGGCAGGGATGAAA
CCCTGGCCCGCCTGGAGAAAGCTGCGGGGTAG

Upstream 100 bases:

>100_bases
GCACAAGCTCAGGCAGCCCGCGCGACGCAGCGTCGGAACCGGCACGGCAAAAAATTTCTTTCGTAGAAAGTTAAAACCAA
TCTGCTCCAAGGATGACAAC

Downstream 100 bases:

>100_bases
GCATCCATTCAGACTGTATTGCGCCGGAGCAAGAGAGGACGTCCACATCAGGGCGTCCTTTTTTTAGAGCAATTTAACTT
TTACATTGCTCTGACGCTTC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTQVVTRFAPSPTGHLHIGGARTAIFCWLLARHFNGRFHLRIEDTDLLRSKQEYTDSILASMRWLGLDWDGELTYQTQRT
DIYNAYVDKLLENGHAYWCSCTPETVEAMREEARAKGLKPRYNGCCRNRDLGPGEGHCVRLKAPLSGKVVFDDLVKGKIA
VDVGELDDMVIRRADGMPTYNMAVVVDDHDMGITHVIRGDDHVSNTPRQILIYEALGLSVPRFGHVPMILGPDRQKLSKR
HGARAVIEYQQDGLLPQALVNYLVRLGWSHGNQELFTPEELVEYFDGTNLNPAAAAFDPTKLEWCNAHFMREMPLAELAA
LTAPFVEQAGLGSLPQERLEPLCAMFRERANNLKALAESFRPLLVPAAELEYAEKDAAKHLAEAGKAHLTALAAVFAACD
PFTAENLEAALNAYVADNGLKFKEVAPPLRTALMGFMGGSHLNEIMAFLGRDETLARLEKAAG

Sequences:

>Translated_463_residues
MTQVVTRFAPSPTGHLHIGGARTAIFCWLLARHFNGRFHLRIEDTDLLRSKQEYTDSILASMRWLGLDWDGELTYQTQRT
DIYNAYVDKLLENGHAYWCSCTPETVEAMREEARAKGLKPRYNGCCRNRDLGPGEGHCVRLKAPLSGKVVFDDLVKGKIA
VDVGELDDMVIRRADGMPTYNMAVVVDDHDMGITHVIRGDDHVSNTPRQILIYEALGLSVPRFGHVPMILGPDRQKLSKR
HGARAVIEYQQDGLLPQALVNYLVRLGWSHGNQELFTPEELVEYFDGTNLNPAAAAFDPTKLEWCNAHFMREMPLAELAA
LTAPFVEQAGLGSLPQERLEPLCAMFRERANNLKALAESFRPLLVPAAELEYAEKDAAKHLAEAGKAHLTALAAVFAACD
PFTAENLEAALNAYVADNGLKFKEVAPPLRTALMGFMGGSHLNEIMAFLGRDETLARLEKAAG
>Mature_462_residues
TQVVTRFAPSPTGHLHIGGARTAIFCWLLARHFNGRFHLRIEDTDLLRSKQEYTDSILASMRWLGLDWDGELTYQTQRTD
IYNAYVDKLLENGHAYWCSCTPETVEAMREEARAKGLKPRYNGCCRNRDLGPGEGHCVRLKAPLSGKVVFDDLVKGKIAV
DVGELDDMVIRRADGMPTYNMAVVVDDHDMGITHVIRGDDHVSNTPRQILIYEALGLSVPRFGHVPMILGPDRQKLSKRH
GARAVIEYQQDGLLPQALVNYLVRLGWSHGNQELFTPEELVEYFDGTNLNPAAAAFDPTKLEWCNAHFMREMPLAELAAL
TAPFVEQAGLGSLPQERLEPLCAMFRERANNLKALAESFRPLLVPAAELEYAEKDAAKHLAEAGKAHLTALAAVFAACDP
FTAENLEAALNAYVADNGLKFKEVAPPLRTALMGFMGGSHLNEIMAFLGRDETLARLEKAAG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=490, Percent_Identity=31.6326530612245, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI62241042, Length=232, Percent_Identity=26.7241379310345, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1788743, Length=462, Percent_Identity=46.5367965367965, Blast_Score=407, Evalue=1e-114,
Organism=Escherichia coli, GI226510925, Length=247, Percent_Identity=38.4615384615385, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1786895, Length=130, Percent_Identity=34.6153846153846, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71993394, Length=478, Percent_Identity=30.7531380753138, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=492, Percent_Identity=32.3170731707317, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=240, Percent_Identity=29.1666666666667, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=136, Percent_Identity=33.8235294117647, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=32.9938900203666, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 51365; Mature: 51234

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVVTRFAPSPTGHLHIGGARTAIFCWLLARHFNGRFHLRIEDTDLLRSKQEYTDSILA
CCCCHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHH
SMRWLGLDWDGELTYQTQRTDIYNAYVDKLLENGHAYWCSCTPETVEAMREEARAKGLKP
HHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCC
RYNGCCRNRDLGPGEGHCVRLKAPLSGKVVFDDLVKGKIAVDVGELDDMVIRRADGMPTY
CCCCCCCCCCCCCCCCCEEEEECCCCCCEEHHHHHCCEEEEEHHHHHHHHHHHCCCCCCE
NMAVVVDDHDMGITHVIRGDDHVSNTPRQILIYEALGLSVPRFGHVPMILGPDRQKLSKR
EEEEEEECCCCCEEEEECCCCCCCCCCCEEEEEEHHCCCCCCCCCCCEEECCCHHHHHHH
HGARAVIEYQQDGLLPQALVNYLVRLGWSHGNQELFTPEELVEYFDGTNLNPAAAAFDPT
CCCCEEEEEHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
KLEWCNAHFMREMPLAELAALTAPFVEQAGLGSLPQERLEPLCAMFRERANNLKALAESF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
RPLLVPAAELEYAEKDAAKHLAEAGKAHLTALAAVFAACDPFTAENLEAALNAYVADNGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
KFKEVAPPLRTALMGFMGGSHLNEIMAFLGRDETLARLEKAAG
CHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
TQVVTRFAPSPTGHLHIGGARTAIFCWLLARHFNGRFHLRIEDTDLLRSKQEYTDSILA
CCCHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHH
SMRWLGLDWDGELTYQTQRTDIYNAYVDKLLENGHAYWCSCTPETVEAMREEARAKGLKP
HHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCC
RYNGCCRNRDLGPGEGHCVRLKAPLSGKVVFDDLVKGKIAVDVGELDDMVIRRADGMPTY
CCCCCCCCCCCCCCCCCEEEEECCCCCCEEHHHHHCCEEEEEHHHHHHHHHHHCCCCCCE
NMAVVVDDHDMGITHVIRGDDHVSNTPRQILIYEALGLSVPRFGHVPMILGPDRQKLSKR
EEEEEEECCCCCEEEEECCCCCCCCCCCEEEEEEHHCCCCCCCCCCCEEECCCHHHHHHH
HGARAVIEYQQDGLLPQALVNYLVRLGWSHGNQELFTPEELVEYFDGTNLNPAAAAFDPT
CCCCEEEEEHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
KLEWCNAHFMREMPLAELAALTAPFVEQAGLGSLPQERLEPLCAMFRERANNLKALAESF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
RPLLVPAAELEYAEKDAAKHLAEAGKAHLTALAAVFAACDPFTAENLEAALNAYVADNGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
KFKEVAPPLRTALMGFMGGSHLNEIMAFLGRDETLARLEKAAG
CHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA