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The map label for this gene is gap [H]

Identifier: 220903418

GI number: 220903418

Start: 168871

End: 169875

Strand: Direct

Name: gap [H]

Synonym: Ddes_0132

Alternate gene names: 220903418

Gene position: 168871-169875 (Clockwise)

Preceding gene: 220903417

Following gene: 220903426

Centisome position: 5.88

GC content: 61.09

Gene sequence:

>1005_bases
ATGCCCGTCAAACTGGGATTGAACGGCTTTGGCCGTATCGGCCGCTACCTGCTGCGGCTGCTGGCCGATGACCAGGATCT
GCAAATCGTCGCCATCAACGCGCGCGCCGATAATGCCGCCCTGGCCTATCTTTTCAAGTACGATTCCACGTACGGCAATT
TTGCGGGCACGGTGGACCATGATGAAAACGGCATTATCGTCAATGGCCGTCACATCGCCGTTACCCGCTGCAAGCCCGGC
GAATGGGAATGGAAGCGCCTTGGCGTGACCATTGCCGTGGAAACCACGGGGACCATCAAGGACGGCGAAGGCCTCGCCAT
GCACCTGGCCTGTGGCGCGGAAAAGGTCGTTATTTCTGCTCCGGCCAAGGATGTGGACGCCATGATAGTTATGGGCGTCA
ACGATCATGTCTATGACTGCGCCAGACACAAGGTCATTTCCGCCGCGTCCTGCACCACCAACTGCCTGGCCCCCGTGGTC
AAGGTGCTGCACGAAAAGTTCGGCATCCGTCACGGCCTCATGACCACCATCCACAGCTACACCATGAGCCAGCGTATTCT
GGACGGTTCACACAAGGACTGGCGCCGGGGCCGCTCGGCTGCCGTGTCAATGGTTCCTTCCAGCACGGGCGCGGCCAAGG
CCGTGGGTGTGGTGATGCCCGAGCTTGAAGGCAAGCTCAACGGCATGTCCGTGCGCGTTCCTACCTTTGCCTGCTCTCTG
GTGGACCTGACCTGCGAGGTCGAGCGTTCCTGTGACGCCGCCGCCGTCAATGCGGCCCTCAAGGCTGCCAGCGAAGGCGT
CATGGGACCGAATATGGGCTTTTCTGAAGAGCCGCTGGTCTCCATCGACTACCGGGGCAGTACCCACGGCGGCGTGGTGG
ACGCGCTCTCCACCCAGGTGCTGGACGGCAGCATGGTCAAGGTGCTGATCTGGTACGATAACGAAGCGGGTTTTACCAAC
CAGCTTTTGCGCCTGTTGCGTATGGTGGGCAGCCGCTGCCGCTAG

Upstream 100 bases:

>100_bases
CCGGGAAGCTGTAAGGCAAATGGTGGCGCACAAGATCCGCACTGTTATGGGATCTTCAGGCAAAGCCTAAACAACATACG
TTTTCGTCAAGGAGCGCAAC

Downstream 100 bases:

>100_bases
AGCGGCATGAAACAGCGTCTCTGAGGCGGTGCGGGCTTTCCGCGCCGCCTTTTTTGTGGCCTGGCTGTAGTGTTCCGACA
GGTTGTTCACCCTCCCCAAA

Product: glyceraldehyde-3-phosphate dehydrogenase, type I

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MPVKLGLNGFGRIGRYLLRLLADDQDLQIVAINARADNAALAYLFKYDSTYGNFAGTVDHDENGIIVNGRHIAVTRCKPG
EWEWKRLGVTIAVETTGTIKDGEGLAMHLACGAEKVVISAPAKDVDAMIVMGVNDHVYDCARHKVISAASCTTNCLAPVV
KVLHEKFGIRHGLMTTIHSYTMSQRILDGSHKDWRRGRSAAVSMVPSSTGAAKAVGVVMPELEGKLNGMSVRVPTFACSL
VDLTCEVERSCDAAAVNAALKAASEGVMGPNMGFSEEPLVSIDYRGSTHGGVVDALSTQVLDGSMVKVLIWYDNEAGFTN
QLLRLLRMVGSRCR

Sequences:

>Translated_334_residues
MPVKLGLNGFGRIGRYLLRLLADDQDLQIVAINARADNAALAYLFKYDSTYGNFAGTVDHDENGIIVNGRHIAVTRCKPG
EWEWKRLGVTIAVETTGTIKDGEGLAMHLACGAEKVVISAPAKDVDAMIVMGVNDHVYDCARHKVISAASCTTNCLAPVV
KVLHEKFGIRHGLMTTIHSYTMSQRILDGSHKDWRRGRSAAVSMVPSSTGAAKAVGVVMPELEGKLNGMSVRVPTFACSL
VDLTCEVERSCDAAAVNAALKAASEGVMGPNMGFSEEPLVSIDYRGSTHGGVVDALSTQVLDGSMVKVLIWYDNEAGFTN
QLLRLLRMVGSRCR
>Mature_333_residues
PVKLGLNGFGRIGRYLLRLLADDQDLQIVAINARADNAALAYLFKYDSTYGNFAGTVDHDENGIIVNGRHIAVTRCKPGE
WEWKRLGVTIAVETTGTIKDGEGLAMHLACGAEKVVISAPAKDVDAMIVMGVNDHVYDCARHKVISAASCTTNCLAPVVK
VLHEKFGIRHGLMTTIHSYTMSQRILDGSHKDWRRGRSAAVSMVPSSTGAAKAVGVVMPELEGKLNGMSVRVPTFACSLV
DLTCEVERSCDAAAVNAALKAASEGVMGPNMGFSEEPLVSIDYRGSTHGGVVDALSTQVLDGSMVKVLIWYDNEAGFTNQ
LLRLLRMVGSRCR

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7657116, Length=331, Percent_Identity=45.0151057401813, Blast_Score=298, Evalue=3e-81,
Organism=Homo sapiens, GI7669492, Length=333, Percent_Identity=45.045045045045, Blast_Score=284, Evalue=1e-76,
Organism=Escherichia coli, GI1788079, Length=328, Percent_Identity=47.2560975609756, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1789295, Length=328, Percent_Identity=37.5, Blast_Score=237, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI17534677, Length=336, Percent_Identity=43.452380952381, Blast_Score=274, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI17534679, Length=336, Percent_Identity=43.452380952381, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI32566163, Length=337, Percent_Identity=42.433234421365, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17568413, Length=337, Percent_Identity=42.433234421365, Blast_Score=271, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6322409, Length=330, Percent_Identity=46.0606060606061, Blast_Score=302, Evalue=6e-83,
Organism=Saccharomyces cerevisiae, GI6322468, Length=330, Percent_Identity=44.8484848484849, Blast_Score=296, Evalue=5e-81,
Organism=Saccharomyces cerevisiae, GI6321631, Length=330, Percent_Identity=45.1515151515152, Blast_Score=295, Evalue=5e-81,
Organism=Drosophila melanogaster, GI85725000, Length=332, Percent_Identity=43.9759036144578, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI22023983, Length=332, Percent_Identity=43.9759036144578, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI19922412, Length=325, Percent_Identity=45.2307692307692, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI17933600, Length=332, Percent_Identity=43.9759036144578, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI18110149, Length=332, Percent_Identity=43.9759036144578, Blast_Score=265, Evalue=3e-71,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35911; Mature: 35780

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKLGLNGFGRIGRYLLRLLADDQDLQIVAINARADNAALAYLFKYDSTYGNFAGTVDH
CCEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCEEEEE
DENGIIVNGRHIAVTRCKPGEWEWKRLGVTIAVETTGTIKDGEGLAMHLACGAEKVVISA
CCCCEEECCCEEEEEECCCCCCCEEEEEEEEEEEECCCEECCCCEEEEEECCCCEEEEEC
PAKDVDAMIVMGVNDHVYDCARHKVISAASCTTNCLAPVVKVLHEKFGIRHGLMTTIHSY
CCCCCCEEEEECCCCHHHHHHHHHHEEHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TMSQRILDGSHKDWRRGRSAAVSMVPSSTGAAKAVGVVMPELEGKLNGMSVRVPTFACSL
HHHHHHHCCCCHHHHCCCCCEEEECCCCCCCHHHHEEEECCCCCCCCCEEEECCCEEEEE
VDLTCEVERSCDAAAVNAALKAASEGVMGPNMGFSEEPLVSIDYRGSTHGGVVDALSTQV
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
LDGSMVKVLIWYDNEAGFTNQLLRLLRMVGSRCR
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVKLGLNGFGRIGRYLLRLLADDQDLQIVAINARADNAALAYLFKYDSTYGNFAGTVDH
CEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCEEEEE
DENGIIVNGRHIAVTRCKPGEWEWKRLGVTIAVETTGTIKDGEGLAMHLACGAEKVVISA
CCCCEEECCCEEEEEECCCCCCCEEEEEEEEEEEECCCEECCCCEEEEEECCCCEEEEEC
PAKDVDAMIVMGVNDHVYDCARHKVISAASCTTNCLAPVVKVLHEKFGIRHGLMTTIHSY
CCCCCCEEEEECCCCHHHHHHHHHHEEHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TMSQRILDGSHKDWRRGRSAAVSMVPSSTGAAKAVGVVMPELEGKLNGMSVRVPTFACSL
HHHHHHHCCCCHHHHCCCCCEEEECCCCCCCHHHHEEEECCCCCCCCCEEEECCCEEEEE
VDLTCEVERSCDAAAVNAALKAASEGVMGPNMGFSEEPLVSIDYRGSTHGGVVDALSTQV
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
LDGSMVKVLIWYDNEAGFTNQLLRLLRMVGSRCR
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2684782; 2227448; 2656407; 7408868; 193030; 3586018; 9175858 [H]