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The map label for this gene is grdE [H]

Identifier: 220903398

GI number: 220903398

Start: 144921

End: 146207

Strand: Direct

Name: grdE [H]

Synonym: Ddes_0112

Alternate gene names: 220903398

Gene position: 144921-146207 (Clockwise)

Preceding gene: 220903397

Following gene: 220903399

Centisome position: 5.04

GC content: 62.47

Gene sequence:

>1287_bases
GTGAAGTTGGAACTACACCGCATCATGGTCAGCAAGCTGGCCTTCGGCGCGCACACCGGCATCCGCGACGGCGTGCTTAC
CGTCAACCGGGAAGAGCTGACAGCCCTGCTCAAGCAGGACGAACGCCTTGACAGTGTCGAGATTGACGCCGCTCACCCCG
GCGAAAGCGTGCGCATCATGCCCGTGAAAGACGCTATTGAACCCCGCTGCAAGCTGGAAGGCCCGGGAGAGGTATTTCCC
GGCTGGATCGGCGATGTGGAAAATGCCGGGGAGGGCAAGACGCTGGTACTGACCGGCATGGCCGTGCTGACCACGGGGCG
CGTGGTGGCCCCGCAGGAAGGCATCGTGGACATGAGCGGCCCCGGCGCCGACTACACCCCTTTTGCAAAGACCTGCAACC
TGACCCTTGCGCTGTCTGCCGCCGCCGACCTTGAGCCGCACCAGTGCGAGGCCTGCTTTCGCCTGGCAGGCCTGCGCGCG
GCCCATTATCTGGCCGCCGCCTGCAAGGAGGCCACAGCGGACAAGGTGGAAGAGTTTGATTTTCCGCCATTCAGCGAAGC
CATGCACGCCCATCCCGGCCTGCCCAGGGTGGCCTATATCTACATGCTGCAGTCCCAGGGCCTGCTGCACGATACCTGGG
TTTACGGCGTGGACGCCAAGCGCATCCTGCCGACCATGATCAGCCCCACGGAAGTTATGGACGGGGCCATCATATCCGGC
AACTGCGTGTCCGCCTGCGACAAGAACAATACCTACGTGCACCTGAACAATCCTGTTATCCGCAGCCTGTACGATCATCA
CGGCAAGGACCTGAACTTTGTCGGCGTGATCATCACCAACGAAAACGTGACCCTGGCGGACAAGAAGCGCAGCTCTTCCT
ATGCGGTCAAGCTGGCGCGCATGATGGGCGTTGATGCCGTGGTCATCAGCGAAGAGGGTTTCGGCAATCCCGATGCCGAC
CTCATCATGAACTGCCGCAAGTCCGAGCAGGCGGGCATCAGAACCGTGCTCATCACCGACGAATTCGCGGGACGTGACGG
CTCAAGCCAGTCTCTGGCCGACTCCTGCCCCGAGGGCGATGCCTGCGTGACCGCAGGCAATGCCAACGAGGTCATCATCC
TGCCGCCCATGCAGAAAATCATCGGCGATCAGGCCCCGGCCGAAACCATAGCGGGCGGCTTCTTCGGCTCCGTGCGCGAG
GACGGCAGCCTTGAAGTGGAGTTGCAGGCCATACTCGGCGCCACGAACGAACTGGGCTTTAACCGCATTGGCGGCCGCAC
CCTGTAG

Upstream 100 bases:

>100_bases
ATCGAGAGTTTGAACTGTAGCATCGCAAGCCTTTCGCCCCCCCACTGGAGGCCATCCGGTTCAGAACCGGCTAGGCTTCC
GCACGAAAAGGAGATGTAAC

Downstream 100 bases:

>100_bases
GGGCGTCCGGCTCAAGGAGCAGGAGACTATCATGGCATATAAGCTTGTGCATTATATAAATCAGTTTTTTGCCGGTATCG
GCGGTGAAGACAAGGCCGAC

Product: Sarcosine reductase

Products: NA

Alternate protein names: Selenoprotein PB alpha/beta [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKLELHRIMVSKLAFGAHTGIRDGVLTVNREELTALLKQDERLDSVEIDAAHPGESVRIMPVKDAIEPRCKLEGPGEVFP
GWIGDVENAGEGKTLVLTGMAVLTTGRVVAPQEGIVDMSGPGADYTPFAKTCNLTLALSAAADLEPHQCEACFRLAGLRA
AHYLAAACKEATADKVEEFDFPPFSEAMHAHPGLPRVAYIYMLQSQGLLHDTWVYGVDAKRILPTMISPTEVMDGAIISG
NCVSACDKNNTYVHLNNPVIRSLYDHHGKDLNFVGVIITNENVTLADKKRSSSYAVKLARMMGVDAVVISEEGFGNPDAD
LIMNCRKSEQAGIRTVLITDEFAGRDGSSQSLADSCPEGDACVTAGNANEVIILPPMQKIIGDQAPAETIAGGFFGSVRE
DGSLEVELQAILGATNELGFNRIGGRTL

Sequences:

>Translated_428_residues
MKLELHRIMVSKLAFGAHTGIRDGVLTVNREELTALLKQDERLDSVEIDAAHPGESVRIMPVKDAIEPRCKLEGPGEVFP
GWIGDVENAGEGKTLVLTGMAVLTTGRVVAPQEGIVDMSGPGADYTPFAKTCNLTLALSAAADLEPHQCEACFRLAGLRA
AHYLAAACKEATADKVEEFDFPPFSEAMHAHPGLPRVAYIYMLQSQGLLHDTWVYGVDAKRILPTMISPTEVMDGAIISG
NCVSACDKNNTYVHLNNPVIRSLYDHHGKDLNFVGVIITNENVTLADKKRSSSYAVKLARMMGVDAVVISEEGFGNPDAD
LIMNCRKSEQAGIRTVLITDEFAGRDGSSQSLADSCPEGDACVTAGNANEVIILPPMQKIIGDQAPAETIAGGFFGSVRE
DGSLEVELQAILGATNELGFNRIGGRTL
>Mature_428_residues
MKLELHRIMVSKLAFGAHTGIRDGVLTVNREELTALLKQDERLDSVEIDAAHPGESVRIMPVKDAIEPRCKLEGPGEVFP
GWIGDVENAGEGKTLVLTGMAVLTTGRVVAPQEGIVDMSGPGADYTPFAKTCNLTLALSAAADLEPHQCEACFRLAGLRA
AHYLAAACKEATADKVEEFDFPPFSEAMHAHPGLPRVAYIYMLQSQGLLHDTWVYGVDAKRILPTMISPTEVMDGAIISG
NCVSACDKNNTYVHLNNPVIRSLYDHHGKDLNFVGVIITNENVTLADKKRSSSYAVKLARMMGVDAVVISEEGFGNPDAD
LIMNCRKSEQAGIRTVLITDEFAGRDGSSQSLADSCPEGDACVTAGNANEVIILPPMQKIIGDQAPAETIAGGFFGSVRE
DGSLEVELQAILGATNELGFNRIGGRTL

Specific function: In the first step of glycine reductase, the substrate is bound to component PB via a Schiff base intermediate. Then the PB- activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated seleno

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016585
- InterPro:   IPR015417 [H]

Pfam domain/function: PF09338 Gly_reductase [H]

EC number: =1.21.4.2 [H]

Molecular weight: Translated: 45835; Mature: 45835

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLELHRIMVSKLAFGAHTGIRDGVLTVNREELTALLKQDERLDSVEIDAAHPGESVRIM
CCCHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHCCCCCEEEEECCCCCCCEEEE
PVKDAIEPRCKLEGPGEVFPGWIGDVENAGEGKTLVLTGMAVLTTGRVVAPQEGIVDMSG
ECHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEECEEHHHCCCEECCCCCEEECCC
PGADYTPFAKTCNLTLALSAAADLEPHQCEACFRLAGLRAAHYLAAACKEATADKVEEFD
CCCCCCCCHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FPPFSEAMHAHPGLPRVAYIYMLQSQGLLHDTWVYGVDAKRILPTMISPTEVMDGAIISG
CCCHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHCCCHHHHCCEEECC
NCVSACDKNNTYVHLNNPVIRSLYDHHGKDLNFVGVIITNENVTLADKKRSSSYAVKLAR
CCHHHCCCCCCEEEECCHHHHHHHHHCCCCCEEEEEEEECCCEEEECCCCCCHHHHHHHH
MMGVDAVVISEEGFGNPDADLIMNCRKSEQAGIRTVLITDEFAGRDGSSQSLADSCPEGD
HHCCCEEEEECCCCCCCCHHHHEECCCCCCCCEEEEEEECCCCCCCCCCCHHHHCCCCCC
ACVTAGNANEVIILPPMQKIIGDQAPAETIAGGFFGSVREDGSLEVELQAILGATNELGF
EEEECCCCCCEEEECCHHHHHCCCCCHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCC
NRIGGRTL
CCCCCCCC
>Mature Secondary Structure
MKLELHRIMVSKLAFGAHTGIRDGVLTVNREELTALLKQDERLDSVEIDAAHPGESVRIM
CCCHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHCCCCCEEEEECCCCCCCEEEE
PVKDAIEPRCKLEGPGEVFPGWIGDVENAGEGKTLVLTGMAVLTTGRVVAPQEGIVDMSG
ECHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEECEEHHHCCCEECCCCCEEECCC
PGADYTPFAKTCNLTLALSAAADLEPHQCEACFRLAGLRAAHYLAAACKEATADKVEEFD
CCCCCCCCHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FPPFSEAMHAHPGLPRVAYIYMLQSQGLLHDTWVYGVDAKRILPTMISPTEVMDGAIISG
CCCHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHCCCHHHHCCEEECC
NCVSACDKNNTYVHLNNPVIRSLYDHHGKDLNFVGVIITNENVTLADKKRSSSYAVKLAR
CCHHHCCCCCCEEEECCHHHHHHHHHCCCCCEEEEEEEECCCEEEECCCCCCHHHHHHHH
MMGVDAVVISEEGFGNPDADLIMNCRKSEQAGIRTVLITDEFAGRDGSSQSLADSCPEGD
HHCCCEEEEECCCCCCCCHHHHEECCCCCCCCEEEEEEECCCCCCCCCCCHHHHCCCCCC
ACVTAGNANEVIILPPMQKIIGDQAPAETIAGGFFGSVREDGSLEVELQAILGATNELGF
EEEECCCCCCEEEECCHHHHHCCCCCHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCC
NRIGGRTL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11271425 [H]