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The map label for this gene is hisF

Identifier: 220903377

GI number: 220903377

Start: 118521

End: 119300

Strand: Direct

Name: hisF

Synonym: Ddes_0091

Alternate gene names: 220903377

Gene position: 118521-119300 (Clockwise)

Preceding gene: 220903376

Following gene: 220903378

Centisome position: 4.12

GC content: 61.79

Gene sequence:

>780_bases
ATGCTCAGTAAACGGGTCATCCCCTGTCTTGATGTACGCAACGGCCGCCTGACCAAGGGGGTCAAGTTTGTGGGCAACGA
GGATATCGGCGATCCCGTGGAAAGCGCCCGCCGCTACTACGAAGAAGGCGCGGACGAGATCGTTTTTTACGACATCACGG
CGTCAGCCGAGGCGCGCGGTATTTTCCTGGATGTTGTGGAGCGCGTGGCTGAACAGATATTCATCCCCTTTTCCGTAGGT
GGCGGCATTTCCAGCGTGGCCGATATGCGGGCCGTACTGCTGGCCGGGGCGGAGAAAGTCTCCATCAATTCTGCGGCGGT
GAAAAATCCCCGGCTTATCGGCGACGGGGCGGATGCCTTCGGTTCCCAGGCTGTCGTGGTAGGCATGGATGTGCTGGCCG
TGCCGGAAAGTCCAGAAATTCCTTCCGGCTACGAGATTGTCATCCACGGCGGGCGCAAGCGCATGGGGCTGGACGCCATC
GCCTGGGCGCGCTGCTGTCAGGAACTGGGCGCGGGCGAACTGTGCGTGAACTCCATTGATGCCGACGGCACCAAGGACGG
CTACGAACTGAAGCTTACCCGTGCTATTGCCGATGCCGTTTCTTTGCCGGTCATTGCTTCCGGTGGGGCGGGCGAACCCC
GGCACATGCTGGAGGCCGTGACCGGCGGCGGGGCCTCGGCGGCACTTATCGCCTCCATTGTACATTACGGGCAGTACAGC
ATACGCCAGTGCAAGGAGTATATGGCCGCCCACGGGGCGCGGATGCGCCTGACCTGGTAA

Upstream 100 bases:

>100_bases
TGGGCCGCGCAGTTTCATCCCGAAAAAAGCGGCAGGCCCGGCTTGCGCCTGCTGGGCAATTTTTACGAATATTGCCGTCA
GTCCCGGCAGGAGGCGCGCC

Downstream 100 bases:

>100_bases
CGGCAGCCCGCCAGCAGCCGACCGGATGGTTCCGGATGGGCGCCGGATGGCTGCCCACGAGGTATCGTGGGCGAAATATT
TCCGGAAGACGGGCTTGATC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLSKRVIPCLDVRNGRLTKGVKFVGNEDIGDPVESARRYYEEGADEIVFYDITASAEARGIFLDVVERVAEQIFIPFSVG
GGISSVADMRAVLLAGAEKVSINSAAVKNPRLIGDGADAFGSQAVVVGMDVLAVPESPEIPSGYEIVIHGGRKRMGLDAI
AWARCCQELGAGELCVNSIDADGTKDGYELKLTRAIADAVSLPVIASGGAGEPRHMLEAVTGGGASAALIASIVHYGQYS
IRQCKEYMAAHGARMRLTW

Sequences:

>Translated_259_residues
MLSKRVIPCLDVRNGRLTKGVKFVGNEDIGDPVESARRYYEEGADEIVFYDITASAEARGIFLDVVERVAEQIFIPFSVG
GGISSVADMRAVLLAGAEKVSINSAAVKNPRLIGDGADAFGSQAVVVGMDVLAVPESPEIPSGYEIVIHGGRKRMGLDAI
AWARCCQELGAGELCVNSIDADGTKDGYELKLTRAIADAVSLPVIASGGAGEPRHMLEAVTGGGASAALIASIVHYGQYS
IRQCKEYMAAHGARMRLTW
>Mature_259_residues
MLSKRVIPCLDVRNGRLTKGVKFVGNEDIGDPVESARRYYEEGADEIVFYDITASAEARGIFLDVVERVAEQIFIPFSVG
GGISSVADMRAVLLAGAEKVSINSAAVKNPRLIGDGADAFGSQAVVVGMDVLAVPESPEIPSGYEIVIHGGRKRMGLDAI
AWARCCQELGAGELCVNSIDADGTKDGYELKLTRAIADAVSLPVIASGGAGEPRHMLEAVTGGGASAALIASIVHYGQYS
IRQCKEYMAAHGARMRLTW

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=261, Percent_Identity=44.0613026819923, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=23.4567901234568, Blast_Score=65, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=34.2857142857143, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_DESDA (B8J216)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002478689.1
- ProteinModelPortal:   B8J216
- GeneID:   7283743
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0091
- HOGENOM:   HBG541613
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27387; Mature: 27387

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKRVIPCLDVRNGRLTKGVKFVGNEDIGDPVESARRYYEEGADEIVFYDITASAEARG
CCCCCCCCEEECCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCH
IFLDVVERVAEQIFIPFSVGGGISSVADMRAVLLAGAEKVSINSAAVKNPRLIGDGADAF
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCEEECCHHHCCCCEEECCCCHHC
GSQAVVVGMDVLAVPESPEIPSGYEIVIHGGRKRMGLDAIAWARCCQELGAGELCVNSID
CCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCC
ADGTKDGYELKLTRAIADAVSLPVIASGGAGEPRHMLEAVTGGGASAALIASIVHYGQYS
CCCCCCCEEEEHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHH
IRQCKEYMAAHGARMRLTW
HHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MLSKRVIPCLDVRNGRLTKGVKFVGNEDIGDPVESARRYYEEGADEIVFYDITASAEARG
CCCCCCCCEEECCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCH
IFLDVVERVAEQIFIPFSVGGGISSVADMRAVLLAGAEKVSINSAAVKNPRLIGDGADAF
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCEEECCHHHCCCCEEECCCCHHC
GSQAVVVGMDVLAVPESPEIPSGYEIVIHGGRKRMGLDAIAWARCCQELGAGELCVNSID
CCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCC
ADGTKDGYELKLTRAIADAVSLPVIASGGAGEPRHMLEAVTGGGASAALIASIVHYGQYS
CCCCCCCEEEEHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHH
IRQCKEYMAAHGARMRLTW
HHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA