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The map label for this gene is hisH

Identifier: 220903376

GI number: 220903376

Start: 117878

End: 118531

Strand: Direct

Name: hisH

Synonym: Ddes_0090

Alternate gene names: 220903376

Gene position: 117878-118531 (Clockwise)

Preceding gene: 220903371

Following gene: 220903377

Centisome position: 4.1

GC content: 61.16

Gene sequence:

>654_bases
ATGCTGGCCATTCTGGATTACAAGGCGGGCAATCAGACAAGCGTGCGCCGCGCTCTGGAGCACCTCGGCGTACCATGCGC
CATAACAGCCGACCCGGCAATGCTCGAAAGTGCCGCGGGAGTGATCTTTCCTGGTGTGGGCGCGGCTGGGCAGGCCATGT
CTGCACTGGCCGAAGCCGGACTGGACAAGGCCCTGCACCACGTGGCGCGACGCGGTCAGCCTTTGCTCGGCATATGCCTG
GGCTGTCAGATCCTGCTGGAAAGCAGCGAGGAAAATGCCGCAAAAACCCTCGGTATTGTTCCCGGTGTCTGCCGCCGGTT
TGAAGACCATATGCGGCAGGAGGACGGCTCTGCGGCTCCGGTTCCGCATATGGGCTGGAACAGCCTTGAGGCTGTGGCTC
CCTGCGTTCTGCTTGACGGTATTGACCCCGCGTCCGAGTATTATTTTGTACACAGTTACTATGTGGAGCCTGATCCCTCG
CTGGTGCTGGCTACCACTACCTACGGGCGCACGTTCTGTTCCCTTTACGGGCGCGACGGCCTCTGGGCCGCGCAGTTTCA
TCCCGAAAAAAGCGGCAGGCCCGGCTTGCGCCTGCTGGGCAATTTTTACGAATATTGCCGTCAGTCCCGGCAGGAGGCGC
GCCATGCTCAGTAA

Upstream 100 bases:

>100_bases
GGCGCGTTGCCTGCTGCCCTGCAAAAATGTATGCTTGCCGCTTCTCAGCGCACCTGACGGCGGCCTGCGGGATCCGTCGC
ACAACAATGAAGGAAGCACC

Downstream 100 bases:

>100_bases
ACGGGTCATCCCCTGTCTTGATGTACGCAACGGCCGCCTGACCAAGGGGGTCAAGTTTGTGGGCAACGAGGATATCGGCG
ATCCCGTGGAAAGCGCCCGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MLAILDYKAGNQTSVRRALEHLGVPCAITADPAMLESAAGVIFPGVGAAGQAMSALAEAGLDKALHHVARRGQPLLGICL
GCQILLESSEENAAKTLGIVPGVCRRFEDHMRQEDGSAAPVPHMGWNSLEAVAPCVLLDGIDPASEYYFVHSYYVEPDPS
LVLATTTYGRTFCSLYGRDGLWAAQFHPEKSGRPGLRLLGNFYEYCRQSRQEARHAQ

Sequences:

>Translated_217_residues
MLAILDYKAGNQTSVRRALEHLGVPCAITADPAMLESAAGVIFPGVGAAGQAMSALAEAGLDKALHHVARRGQPLLGICL
GCQILLESSEENAAKTLGIVPGVCRRFEDHMRQEDGSAAPVPHMGWNSLEAVAPCVLLDGIDPASEYYFVHSYYVEPDPS
LVLATTTYGRTFCSLYGRDGLWAAQFHPEKSGRPGLRLLGNFYEYCRQSRQEARHAQ
>Mature_217_residues
MLAILDYKAGNQTSVRRALEHLGVPCAITADPAMLESAAGVIFPGVGAAGQAMSALAEAGLDKALHHVARRGQPLLGICL
GCQILLESSEENAAKTLGIVPGVCRRFEDHMRQEDGSAAPVPHMGWNSLEAVAPCVLLDGIDPASEYYFVHSYYVEPDPS
LVLATTTYGRTFCSLYGRDGLWAAQFHPEKSGRPGLRLLGNFYEYCRQSRQEARHAQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=204, Percent_Identity=37.2549019607843, Blast_Score=120, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319725, Length=215, Percent_Identity=33.0232558139535, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_DESDA (B8J215)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002478688.1
- ProteinModelPortal:   B8J215
- GeneID:   7283742
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0090
- HOGENOM:   HBG292341
- ProtClustDB:   PRK13141
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23457; Mature: 23457

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 187-187 ACT_SITE 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAILDYKAGNQTSVRRALEHLGVPCAITADPAMLESAAGVIFPGVGAAGQAMSALAEAG
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHCCCEEECCCCCHHHHHHHHHHHH
LDKALHHVARRGQPLLGICLGCQILLESSEENAAKTLGIVPGVCRRFEDHMRQEDGSAAP
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
VPHMGWNSLEAVAPCVLLDGIDPASEYYFVHSYYVEPDPSLVLATTTYGRTFCSLYGRDG
CCCCCCCCHHHHHHHHEECCCCCCHHEEEEEEEEECCCCCEEEEECCHHHHHHHHHCCCC
LWAAQFHPEKSGRPGLRLLGNFYEYCRQSRQEARHAQ
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAILDYKAGNQTSVRRALEHLGVPCAITADPAMLESAAGVIFPGVGAAGQAMSALAEAG
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHCCCEEECCCCCHHHHHHHHHHHH
LDKALHHVARRGQPLLGICLGCQILLESSEENAAKTLGIVPGVCRRFEDHMRQEDGSAAP
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
VPHMGWNSLEAVAPCVLLDGIDPASEYYFVHSYYVEPDPSLVLATTTYGRTFCSLYGRDG
CCCCCCCCHHHHHHHHEECCCCCCHHEEEEEEEEECCCCCEEEEECCHHHHHHHHHCCCC
LWAAQFHPEKSGRPGLRLLGNFYEYCRQSRQEARHAQ
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA