Click here to switch to the map view.

The map label for this gene is cobB [H]

Identifier: 220903314

GI number: 220903314

Start: 39141

End: 40895

Strand: Reverse

Name: cobB [H]

Synonym: Ddes_0028

Alternate gene names: 220903314

Gene position: 40895-39141 (Counterclockwise)

Preceding gene: 220903315

Following gene: 220903313

Centisome position: 1.42

GC content: 63.36

Gene sequence:

>1755_bases
ATGCACACCGAACAGCCGCGCATTCCCGGCATCATCATCGCCGGCACAGGCAGCAATACGGGCAAGACCACGGCCACCCT
GGCCCTGCTTTGCGCCCTGGCTGCCCGCGGCATTCCCGTGCGCGCCGCCAAAACCGGTCCCGACTATATAGACGCCGCTT
TTCACGCCGCCATCACAGGTCTGCCCGCCGCCAATCTTGACACGTGGATGTGTCGCGAAACTCTGCCCAAAGCCGGAGAA
AGCCCGCTGACTTCGAGAAAACGCCTGCCCAGGGGTCTGCGCAGCGTCTTTGCCCGCATGGCCGGGCCTGAAAACTCTCA
AAAACATTCCTTCGGCACGCCCGCCCCCGCCCTTCTTGTGGTTGAAGGAGCCATGGGCCTGTATGACGGCGGACATGGCG
GCGCGGGCAGCACGGCACAGCTGGCGGCCCTGCTGGACCTGCCCATTCTGCTCATATGCAGTGCGGCAGGGCTTGGGCAG
TCAGTGGCGCCGCTGGTGGAAGGCTATGTCCGCCACAAGCCGCACTGGTTGTCCGTCCACGGTCCAACCCCGCGCTTTCT
GGGCGTTATCTGCACACATGTGGGCAGTCCGCGCCATGCAGAAATTATTGAAGATGCCCTGAAGCCGCTGCAAAAAACAG
ACGGCCTACCCCTGCTGGGGCTGTTGCCCCGCGCTGGAGCACCTGCCCTCAAGTCCCGCCATCTTGGCCTTGTGGAAGCA
CGGGAGGCCCTGTCCGCCCTGGATCGCCCCGCGCTGGCACACTGGTTTGAAGAAAATTGCCGCCTGGATGCCCTGCTGCG
TCTGGTGGGAACAGCAGACAGTCTGCACAATACGCCAGATGCGGCCGCTTTTTTTATGGCAAATGCGGAGAAAGGCAGCC
CTGCCACAGAAGAAACAGCCTGGGCCGCGCGCTTTTTTATGCCGCGCCCGCCCGGTGGCCGGCATGTTCTTCAAAATGCT
GTTGACGGTGCGGGAATGGGGCAGCACGACGCTCCCGCTGCCCCCCAAAAAACTTCCACCCCCAAACGCACCCGCCGCCC
CCGTGTAGGCATGGCCTGGGATGCGGCCTTCAGTTTCTGCTATGCCGACCTGCCCCCCCTGCTCGCAGAACTGGGCGCAG
AGCTTGTTTTCTTTTCACCGCTGCGCGATGCCGCGCCGCCGCCCGGCTGTTCCGCCCTGTACTTTCCCGGCGGCTACCCG
GAACTGCATGCCCCGCAACTGTCAACCAACGTCCGCATGCTTGACGCCCTGCGCGCACTGGCCCGGAACGGCCTTCCCAT
ATATGGTGAGTGCGGGGGCTACATCTACCTTATGCACTCCCTGCGGCTGAAGACACATGACGGGGCGGACAGAGCAACAG
AACATCCCATGGCCGGTCTTTTACCCATACACTGCACCCTGGGGCAGGAGCGTGCAGCCCTGGGCTACCGGGCCGCTCTG
GCCCTGCCGGGCTGGCCGCAAGACCGGCCCCAGGACCGCGCCCAAACCGGGGCAGGCGCAGAATGCAGCCGCCCTGCCCC
CATCTGGGTACGGGGACACGAATTTCACTATGCCCGTGAAGACGATTCCCCTTTGCCCGCACACTGCGCGCCATTGTGGA
ACCTGTATGACAGCAAGGGAATTTTTTTACGGCAGGAGGGCTGCCGCCACGGCTCCGTGGCCGGAACATGGCTGCACTGC
TATCCTGAGGGATCCCGACGCTTCTGGCGTACATGGCTCGAAGCAGCGGTAAAAAACAATGGCAGAACGATGTAA

Upstream 100 bases:

>100_bases
GTATGCTCAACGCTGACATGTGGCAAAACATCTCGTAAAAGTGAAAAGCTGGATGCAGAAGCTGCTTCCGTCTGGAACCC
CCTGATCCGCCGGAGACGTC

Downstream 100 bases:

>100_bases
CGCCGCGAGCGCACACACGACCAACCGAGGCTTACCCATGACGTATATTCCTGCGCCGGAACATTCCGTAGAACTGGACC
CGGCCTGCACCCCCCAGACC

Product: CobB/CobQ domain-containing protein glutamine amidotransferase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MHTEQPRIPGIIIAGTGSNTGKTTATLALLCALAARGIPVRAAKTGPDYIDAAFHAAITGLPAANLDTWMCRETLPKAGE
SPLTSRKRLPRGLRSVFARMAGPENSQKHSFGTPAPALLVVEGAMGLYDGGHGGAGSTAQLAALLDLPILLICSAAGLGQ
SVAPLVEGYVRHKPHWLSVHGPTPRFLGVICTHVGSPRHAEIIEDALKPLQKTDGLPLLGLLPRAGAPALKSRHLGLVEA
REALSALDRPALAHWFEENCRLDALLRLVGTADSLHNTPDAAAFFMANAEKGSPATEETAWAARFFMPRPPGGRHVLQNA
VDGAGMGQHDAPAAPQKTSTPKRTRRPRVGMAWDAAFSFCYADLPPLLAELGAELVFFSPLRDAAPPPGCSALYFPGGYP
ELHAPQLSTNVRMLDALRALARNGLPIYGECGGYIYLMHSLRLKTHDGADRATEHPMAGLLPIHCTLGQERAALGYRAAL
ALPGWPQDRPQDRAQTGAGAECSRPAPIWVRGHEFHYAREDDSPLPAHCAPLWNLYDSKGIFLRQEGCRHGSVAGTWLHC
YPEGSRRFWRTWLEAAVKNNGRTM

Sequences:

>Translated_584_residues
MHTEQPRIPGIIIAGTGSNTGKTTATLALLCALAARGIPVRAAKTGPDYIDAAFHAAITGLPAANLDTWMCRETLPKAGE
SPLTSRKRLPRGLRSVFARMAGPENSQKHSFGTPAPALLVVEGAMGLYDGGHGGAGSTAQLAALLDLPILLICSAAGLGQ
SVAPLVEGYVRHKPHWLSVHGPTPRFLGVICTHVGSPRHAEIIEDALKPLQKTDGLPLLGLLPRAGAPALKSRHLGLVEA
REALSALDRPALAHWFEENCRLDALLRLVGTADSLHNTPDAAAFFMANAEKGSPATEETAWAARFFMPRPPGGRHVLQNA
VDGAGMGQHDAPAAPQKTSTPKRTRRPRVGMAWDAAFSFCYADLPPLLAELGAELVFFSPLRDAAPPPGCSALYFPGGYP
ELHAPQLSTNVRMLDALRALARNGLPIYGECGGYIYLMHSLRLKTHDGADRATEHPMAGLLPIHCTLGQERAALGYRAAL
ALPGWPQDRPQDRAQTGAGAECSRPAPIWVRGHEFHYAREDDSPLPAHCAPLWNLYDSKGIFLRQEGCRHGSVAGTWLHC
YPEGSRRFWRTWLEAAVKNNGRTM
>Mature_584_residues
MHTEQPRIPGIIIAGTGSNTGKTTATLALLCALAARGIPVRAAKTGPDYIDAAFHAAITGLPAANLDTWMCRETLPKAGE
SPLTSRKRLPRGLRSVFARMAGPENSQKHSFGTPAPALLVVEGAMGLYDGGHGGAGSTAQLAALLDLPILLICSAAGLGQ
SVAPLVEGYVRHKPHWLSVHGPTPRFLGVICTHVGSPRHAEIIEDALKPLQKTDGLPLLGLLPRAGAPALKSRHLGLVEA
REALSALDRPALAHWFEENCRLDALLRLVGTADSLHNTPDAAAFFMANAEKGSPATEETAWAARFFMPRPPGGRHVLQNA
VDGAGMGQHDAPAAPQKTSTPKRTRRPRVGMAWDAAFSFCYADLPPLLAELGAELVFFSPLRDAAPPPGCSALYFPGGYP
ELHAPQLSTNVRMLDALRALARNGLPIYGECGGYIYLMHSLRLKTHDGADRATEHPMAGLLPIHCTLGQERAALGYRAAL
ALPGWPQDRPQDRAQTGAGAECSRPAPIWVRGHEFHYAREDDSPLPAHCAPLWNLYDSKGIFLRQEGCRHGSVAGTWLHC
YPEGSRRFWRTWLEAAVKNNGRTM

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 62658; Mature: 62658

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTEQPRIPGIIIAGTGSNTGKTTATLALLCALAARGIPVRAAKTGPDYIDAAFHAAITG
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHC
LPAANLDTWMCRETLPKAGESPLTSRKRLPRGLRSVFARMAGPENSQKHSFGTPAPALLV
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
VEGAMGLYDGGHGGAGSTAQLAALLDLPILLICSAAGLGQSVAPLVEGYVRHKPHWLSVH
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEC
GPTPRFLGVICTHVGSPRHAEIIEDALKPLQKTDGLPLLGLLPRAGAPALKSRHLGLVEA
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCCHHHH
REALSALDRPALAHWFEENCRLDALLRLVGTADSLHNTPDAAAFFMANAEKGSPATEETA
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCHHHH
WAARFFMPRPPGGRHVLQNAVDGAGMGQHDAPAAPQKTSTPKRTRRPRVGMAWDAAFSFC
HHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHH
YADLPPLLAELGAELVFFSPLRDAAPPPGCSALYFPGGYPELHAPQLSTNVRMLDALRAL
HHHHHHHHHHCCCCEEEECCHHHCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHH
ARNGLPIYGECGGYIYLMHSLRLKTHDGADRATEHPMAGLLPIHCTLGQERAALGYRAAL
HHCCCEEEECCCCEEEEEEHHHEECCCCCCHHHCCCCCCCEEEEEECCCHHHHHHHHEEE
ALPGWPQDRPQDRAQTGAGAECSRPAPIWVRGHEFHYAREDDSPLPAHCAPLWNLYDSKG
ECCCCCCCCCHHHHHCCCCCCCCCCCCEEEECCEEEECCCCCCCCCCHHHHHHHHHCCCC
IFLRQEGCRHGSVAGTWLHCYPEGSRRFWRTWLEAAVKNNGRTM
EEEECCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHTEQPRIPGIIIAGTGSNTGKTTATLALLCALAARGIPVRAAKTGPDYIDAAFHAAITG
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHC
LPAANLDTWMCRETLPKAGESPLTSRKRLPRGLRSVFARMAGPENSQKHSFGTPAPALLV
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
VEGAMGLYDGGHGGAGSTAQLAALLDLPILLICSAAGLGQSVAPLVEGYVRHKPHWLSVH
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEC
GPTPRFLGVICTHVGSPRHAEIIEDALKPLQKTDGLPLLGLLPRAGAPALKSRHLGLVEA
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCCHHHH
REALSALDRPALAHWFEENCRLDALLRLVGTADSLHNTPDAAAFFMANAEKGSPATEETA
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCHHHH
WAARFFMPRPPGGRHVLQNAVDGAGMGQHDAPAAPQKTSTPKRTRRPRVGMAWDAAFSFC
HHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHH
YADLPPLLAELGAELVFFSPLRDAAPPPGCSALYFPGGYPELHAPQLSTNVRMLDALRAL
HHHHHHHHHHCCCCEEEECCHHHCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHH
ARNGLPIYGECGGYIYLMHSLRLKTHDGADRATEHPMAGLLPIHCTLGQERAALGYRAAL
HHCCCEEEECCCCEEEEEEHHHEECCCCCCHHHCCCCCCCEEEEEECCCHHHHHHHHEEE
ALPGWPQDRPQDRAQTGAGAECSRPAPIWVRGHEFHYAREDDSPLPAHCAPLWNLYDSKG
ECCCCCCCCCHHHHHCCCCCCCCCCCCEEEECCEEEECCCCCCCCCCHHHHHHHHHCCCC
IFLRQEGCRHGSVAGTWLHCYPEGSRRFWRTWLEAAVKNNGRTM
EEEECCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2211520 [H]