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The map label for this gene is ytzG [H]

Identifier: 21910600

GI number: 21910600

Start: 1108047

End: 1108712

Strand: Reverse

Name: ytzG [H]

Synonym: SpyM3_1064

Alternate gene names: 21910600

Gene position: 1108712-1108047 (Counterclockwise)

Preceding gene: 21910601

Following gene: 21910599

Centisome position: 58.34

GC content: 42.79

Gene sequence:

>666_bases
GTGAAGAAACTGATTAAGGCTCAAGGGGTTTGGGTTGATCACATGCCTGCCAGAAATGGTCGACAAAATGTTGATCCTGG
CTTGCAACTGATTGAGGTGACTGGACAGCGAGTGACGCATCCTAAACATTCTTACATTATCCTCAACAAGCCATCAGGGG
TGGTCTCAGCTAAGAAAGATACTAACTATTTAACCGTTATTGATCAGCTTGCTGAAGAAGATAAAAGTCCAGACTTGTAT
CCTGTTGGCAGATTGGATAGAGATACCGAAGGGTTGGTTTTACTCACTGATAATGGTCCCCTAGGGTTTCGGATGTTGGA
CCCTAGTCATCATGTTTCTAAAACGTATCTTGTAACCGTTAATGGCTTGTTAGCAGAAGATGCCTCAGATTTTTTTGCAG
CTGGTATTTGTTTCCCAACAGGTGAACAATGCCAACCAGCTCAATTGACTATTCTTAAAGCCGATACAGACCAGTCACAG
GCTAGTTTGACCATTTCAGAAGGAAAATTTCACCAAGTGAAAAAAATGTTTTTGACCTATGGTTTGAAAGTGACCTCTCT
GAAACGGACGCACTTTGCAGGGCTGGAACTCGGTCATTTAGCCAGCGGAGAATATCGTTATCTAACAGCGTCAGAAAGGC
AACTCATTAAAACGTATCTGGACTGA

Upstream 100 bases:

>100_bases
TGTAACTTCTATTTCTTTTGCAAGGGTAAATTTATGTTACACTACTAATATGCGTTTAGATAAATTATTAGAAGGTACAA
AAGTTGGCTCTAGAAGTCAA

Downstream 100 bases:

>100_bases
GCAAAACATGCTTTCAAAAAGAAGCTATTCTTGCTTAATCAATAAAGTGTCCATTGACTATTATAGTCATGATGAGTCCG
ACTTTTGCTATGTTTAGTCA

Product: putative 16S pseudouridylate synthetase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLY
PVGRLDRDTEGLVLLTDNGPLGFRMLDPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQCQPAQLTILKADTDQSQ
ASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRYLTASERQLIKTYLD

Sequences:

>Translated_221_residues
MKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLY
PVGRLDRDTEGLVLLTDNGPLGFRMLDPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQCQPAQLTILKADTDQSQ
ASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRYLTASERQLIKTYLD
>Mature_221_residues
MKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLY
PVGRLDRDTEGLVLLTDNGPLGFRMLDPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQCQPAQLTILKADTDQSQ
ASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRYLTASERQLIKTYLD

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=170, Percent_Identity=41.7647058823529, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1790453, Length=218, Percent_Identity=29.8165137614679, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081838, Length=200, Percent_Identity=28.5, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 24525; Mature: 24525

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKD
CCCCHHHCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCEEECCC
TNYLTVIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGFRMLDPSHHVSKTYLVTV
CCHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCEEEEEECCCCCCCEEEEEEE
NGLLAEDASDFFAAGICFPTGEQCQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTY
CCEECCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHH
GLKVTSLKRTHFAGLELGHLASGEYRYLTASERQLIKTYLD
CCEEEEECCCEECCCEECCCCCCCEEEEECCHHHHHHHHCC
>Mature Secondary Structure
MKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKD
CCCCHHHCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCEEECCC
TNYLTVIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGFRMLDPSHHVSKTYLVTV
CCHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCEEEEEECCCCCCCEEEEEEE
NGLLAEDASDFFAAGICFPTGEQCQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTY
CCEECCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHH
GLKVTSLKRTHFAGLELGHLASGEYRYLTASERQLIKTYLD
CCEEEEECCCEECCCEECCCCCCCEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]