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The map label for this gene is alaS [H]

Identifier: 21910594

GI number: 21910594

Start: 1098502

End: 1100979

Strand: Reverse

Name: alaS [H]

Synonym: SpyM3_1058

Alternate gene names: 21910594

Gene position: 1100979-1098502 (Counterclockwise)

Preceding gene: 21910595

Following gene: 21910593

Centisome position: 57.93

GC content: 42.45

Gene sequence:

>2478_bases
TTGAAAAAATATTTTGATGGTTCAGTGATTCCAGAAAATCCACGTATTACCAATGCACAAAAATCAATTCGTACTAATGA
TATTGAAAATGTTGGTAAAACAGCACGTCACCATACTATGTTTGAAATGCTTGGTAACTTCTCAATTGGAGACTATTTCC
GTGATGAAGCTATTGAGTGGGGATTTGAACTCTTGACAAGTCCAGACTGGTTTGATTTCCCTAAAGACAAGCTCTACATG
ACTTATTACCCAGATGACAAGGATTCGTATAACCGTTGGATTGCTTGTGGCGTTGAACCAAGTCACTTGGTGCCGATCGA
GGATAACTTCTGGGAAATCGGTGCTGGTCCTTCAGGTCCAGATACGGAGATTTTCTTCGACCGTGGTGAAGATTTCGATC
CAGAAAATATCGGACTTCGCCTCTTGGCTGAAGATATCGAAAACGATCGTTACATCGAAATCTGGAACATCGTTCTCTCA
CAATTCAATGCTGACCCAGCCGTACCACGTTCAGAATACAAAGAATTACCAAACAAAAACATTGATACAGGTGCTGGTCT
TGAACGTCTTGCAGCTGTTATGCAAGGGGCAAAAACAAACTTTGAAACTGACCTCTTCATGCCAATCATCCGTGAAGTAG
AGAAGTTGTCAGGTAAAACTTACGATCCAGATGGCGACAACATGAGTTTCAAGGTTATCGCTGACCACATCCGTGCGCTT
TCATTTGCTATCGGTGATGGTGCGCTTCCTGGAAATGAAGGTCGTGGTTACGTTCTTCGTCGTCTTCTCCGTCGTGCGGT
TATGCACGGTCGCCGTCTTGGCATCAACGAAACTTTCCTTTACAAATTGGTTCCAACTGTTGGACAAATCATGGAAAGCT
ACTACCCAGAAGTACTTGAAAAACGTGACTTTATCGAGAAAATCGTTAAACGTGAGGAAGAGACATTTGCTCGTACTATC
GATGCAGGTAGCGGTCACTTAGATTCATTGCTTGCGCAGCTTAAGGCTGAAGGTAAGGATACTCTTGAAGGTAAAGATAT
CTTCAAACTTTATGATACTTATGGATTCCCGGTTGAATTGACAGAGGAATTGGCAGAAGATGCAGGCTACAAGATTGACC
ACGAAGGCTTTAAGTCAGCCATGAAAGAACAACAAGACCGTGCGCGTGCAGCTGTTGTTAAGGGTGGTTCAATGGGGATG
CAAAATGAAACCCTAGCTGGTATTGTTGAAGAATCACGATTTGAATACGACACATATAGTCTTGAATCAAGTCTTTCAGT
CATCATCGCTGATAATGAACGTACCGAAGCTGTTTCAGAAGGTCAAGCTCTTCTTGTCTTTGCTCAAACACCATTCTATG
CTGAAATGGGTGGACAGGTTGCTGACACAGGTAGAATCAAAAATGATAAGGGTGACACAGTTGCTGAGGTTGTTGATGTT
CAAAAAGCACCAAATGGTCAACCTCTACACACTGTAAACGTTTTGGCATCACTTTCAGTTGGAACAAACTACACACTTGA
AATCAACAAAGAGCGTCGTTTGGCTGTTGAGAAAAACCACACAGCTACTCACTTGCTCCATGCAGCTCTTCACAATGTTA
TCGGTGAACACGCAACTCAGGCTGGTTCATTGAACGAAGAAGAATTCTTGCGCTTTGATTTTACTCACTTTGAAGCAGTA
AGCAATGAGGAACTTCGTCACATTGAACAAGAAGTTAATGAGCAAATTTGGAACGCTCTTACAATCACAACGACTGAAAC
TGACGTTGAAACCGCAAAAGAGATGGGAGCAATGGCGCTTTTTGGTGAGAAATATGGTAAAGTGGTTCGTGTGGTTCAAA
TTGGTAATTATTCTGTTGAACTTTGTGGTGGAACTCACTTAAATAATTCTTCAGAAATCGGTCTCTTCAAGATTGTCAAA
GAAGAAGGTATTGGTTCAGGCACTCGTCGTATTATTGCAGTTACTGGTAGACAAGCTTTTGAAGCTTATCGTAACCAAGA
GGATGCCCTAAAAGATATCGCTGCTACTGTAAAAGCTCCGCAATTGAAAGATGCAGCAGCTAAAGTACAAGCTCTTAGCG
ACTCACTTCGTGATCTTCAAAAAGAAAATGCAGAGCTTAAAGAAAAAGCAGCAGCTGCAGCAGCTGGTGATGTCTTTAAA
GATGTTCAAGAAGCTAAGGGCGTGCGCTTCATTGCTAGTCAAGTTGATGTTGCAGATGCAGGGGCACTTCGTACATTTGC
TGATAACTGGAAACAAAAAGACTACTCTGATGTGCTTGTTCTCGTAGCAGCTATTGGTGAGAAGGTTAATGTCCTTGTTG
CAAGCAAAACCAAAGATGTCCACGCTGGTAACATGATCAAAGAATTGGCACCAATTGTAGCAGGTCGTGGTGGAGGTAAA
CCAGACATGGCTATGGCAGGTGGTAGCGATGCAAGTAAAATTGCAGAGCTGCTAGCAGCAGTTGCTGAAATAGTGTAA

Upstream 100 bases:

>100_bases
GGATTTCTGGAAATCTAAAGGACATTGCGTTGAGCCTTCAGCTAACTTGGTTCCTGTGAACGACCCAACGCTTCTTTGGA
TCAACTCAGGTGTTGCAACC

Downstream 100 bases:

>100_bases
ATCCATAAAAACCAAGTTATTTAGAACTTGGTTTTTATATTTGAAATGACTATCATCATAACCTTTTTGACTATCATCGT
TGTCAAAAAGAAAAAGATAT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 825; Mature: 825

Protein sequence:

>825_residues
MKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPDWFDFPKDKLYM
TYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEIGAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLS
QFNADPAVPRSEYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRAL
SFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTVGQIMESYYPEVLEKRDFIEKIVKREEETFARTI
DAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGM
QNETLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDV
QKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAV
SNEELRHIEQEVNEQIWNALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGLFKIVK
EEGIGSGTRRIIAVTGRQAFEAYRNQEDALKDIAATVKAPQLKDAAAKVQALSDSLRDLQKENAELKEKAAAAAAGDVFK
DVQEAKGVRFIASQVDVADAGALRTFADNWKQKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGK
PDMAMAGGSDASKIAELLAAVAEIV

Sequences:

>Translated_825_residues
MKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPDWFDFPKDKLYM
TYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEIGAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLS
QFNADPAVPRSEYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRAL
SFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTVGQIMESYYPEVLEKRDFIEKIVKREEETFARTI
DAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGM
QNETLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDV
QKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAV
SNEELRHIEQEVNEQIWNALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGLFKIVK
EEGIGSGTRRIIAVTGRQAFEAYRNQEDALKDIAATVKAPQLKDAAAKVQALSDSLRDLQKENAELKEKAAAAAAGDVFK
DVQEAKGVRFIASQVDVADAGALRTFADNWKQKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGK
PDMAMAGGSDASKIAELLAAVAEIV
>Mature_825_residues
MKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPDWFDFPKDKLYM
TYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEIGAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLS
QFNADPAVPRSEYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRAL
SFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTVGQIMESYYPEVLEKRDFIEKIVKREEETFARTI
DAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGM
QNETLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDV
QKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAV
SNEELRHIEQEVNEQIWNALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGLFKIVK
EEGIGSGTRRIIAVTGRQAFEAYRNQEDALKDIAATVKAPQLKDAAAKVQALSDSLRDLQKENAELKEKAAAAAAGDVFK
DVQEAKGVRFIASQVDVADAGALRTFADNWKQKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGK
PDMAMAGGSDASKIAELLAAVAEIV

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=932, Percent_Identity=33.7982832618026, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI38569417, Length=728, Percent_Identity=31.3186813186813, Blast_Score=306, Evalue=6e-83,
Organism=Escherichia coli, GI1789048, Length=827, Percent_Identity=43.0471584038694, Blast_Score=621, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI17506981, Length=905, Percent_Identity=31.0497237569061, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17536681, Length=710, Percent_Identity=32.2535211267606, Blast_Score=321, Evalue=1e-87,
Organism=Saccharomyces cerevisiae, GI6324911, Length=767, Percent_Identity=37.6792698826597, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24582809, Length=908, Percent_Identity=32.3788546255507, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45552267, Length=908, Percent_Identity=32.3788546255507, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24658214, Length=769, Percent_Identity=27.9583875162549, Blast_Score=249, Evalue=8e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR016040
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 91255; Mature: 91255

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRDEAIEW
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEIGAGPSGP
HHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCEEEECCCCCEEECCCCCCC
DTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPNKN
CCCEEEECCCCCCCCCCCHHEEEHHCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHCCCCC
IDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRAL
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
SFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTVGQIMESYYPEVLE
HHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDTYGFPVEL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHH
TEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNETLAGIVEESRFEYDTYS
HHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHCCCCCCEE
LESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDV
CCCCCEEEEECCCHHHHHCCCCEEEEEECCCCHHHHCCCEECCCCCCCCCCCHHHHHHHH
QKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKNHTATHLLHAALHNVIGEHATQ
HCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHC
AGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIWNALTITTTETDVETAKEMGAMAL
CCCCCHHHHHEECHHHHHHCCHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHH
FGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGLFKIVKEEGIGSGTRRIIAVTGRQAF
HHHHHCCEEEEEEECCEEEEEECCCCCCCCCCCCCEEEEHHCCCCCCCCEEEEECCHHHH
EAYRNQEDALKDIAATVKAPQLKDAAAKVQALSDSLRDLQKENAELKEKAAAAAAGDVFK
HHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVQEAKGVRFIASQVDVADAGALRTFADNWKQKDYSDVLVLVAAIGEKVNVLVASKTKDV
HHHHHCCCEEHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCC
HAGNMIKELAPIVAGRGGGKPDMAMAGGSDASKIAELLAAVAEIV
HHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRDEAIEW
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEIGAGPSGP
HHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCEEEECCCCCEEECCCCCCC
DTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPNKN
CCCEEEECCCCCCCCCCCHHEEEHHCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHCCCCC
IDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRAL
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
SFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTVGQIMESYYPEVLE
HHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDTYGFPVEL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHH
TEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNETLAGIVEESRFEYDTYS
HHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHCCCCCCEE
LESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDV
CCCCCEEEEECCCHHHHHCCCCEEEEEECCCCHHHHCCCEECCCCCCCCCCCHHHHHHHH
QKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKNHTATHLLHAALHNVIGEHATQ
HCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHC
AGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIWNALTITTTETDVETAKEMGAMAL
CCCCCHHHHHEECHHHHHHCCHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHH
FGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGLFKIVKEEGIGSGTRRIIAVTGRQAF
HHHHHCCEEEEEEECCEEEEEECCCCCCCCCCCCCEEEEHHCCCCCCCCEEEEECCHHHH
EAYRNQEDALKDIAATVKAPQLKDAAAKVQALSDSLRDLQKENAELKEKAAAAAAGDVFK
HHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVQEAKGVRFIASQVDVADAGALRTFADNWKQKDYSDVLVLVAAIGEKVNVLVASKTKDV
HHHHHCCCEEHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCC
HAGNMIKELAPIVAGRGGGKPDMAMAGGSDASKIAELLAAVAEIV
HHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA