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The map label for this gene is papS [H]

Identifier: 21910123

GI number: 21910123

Start: 645296

End: 646504

Strand: Direct

Name: papS [H]

Synonym: SpyM3_0587

Alternate gene names: 21910123

Gene position: 645296-646504 (Clockwise)

Preceding gene: 21910122

Following gene: 21910124

Centisome position: 33.95

GC content: 39.95

Gene sequence:

>1209_bases
ATGAAATTAATGACAATGCCTTCTGAGTTTCAGAAGGCTTTACCCATATTAACTAAAATAAAAGAAGCTGGTTATGAAGC
CTACTTTGTGGGAGGAAGCGTCAGAGATGTTTTACTCGAGCGTCCCATTCATGATGTAGATATTGCAACTAGTTCCTATC
CTGAAGAGACTAAAGCGATTTTTAACAGAACTGTCGATGTTGGCATTGAACATGGTACAGTGCTCGTGCTTGAAAATGGT
GGTGAATATGAAATCACAACCTTTAGGACAGAAGATGTCTATGTTGACTATCGCCGTCCTAGTCAGGTTTCTTTTGTGCG
CTCTTTAGAGGAAGACTTAAAACGAAGAGACTTTACCGTCAATGCCTTGGCCTTGGATGAAAATGGTCAAGTTATTGATA
AATTTAGAGGACTCATTGATTTGGAGCAAAAACGATTACGAGCAGTGGGTAAAGCAGAGGAACGTTTTGAAGAAGATGCA
CTACGCATTATGAGAGGCTTTCGATTTGCGGCGAGTCTGGATTTTGACATTGAAGCTGCAACTTTTGAAGCTATGAGGAG
CCATTCACCTCTATTGGAAAAGATTTCTGTGGAACGTTCTTTTACTGAATTTGATAAATTATTGATGGCACCGCACTGGC
GAAAAGGGATTTCTGCCATGATTGCTTGTCAGGCTTATGACTATTTACCAGGCTTGAAGCAGCAGGAAGCAGGCTTGAAT
CATCTGATAGTTTCTTTGAAAGATAACTTTACCTTTAGTGATCACCACCAAGCTTGGGCTTATGTGATGATTAGTTTAGC
CATTGAGGATCCTAAAAGCTTCCTAAAAGCATGGAAGACTTCAAATGATTTTCAGCGTTATGTCACAAAGTTGATTGCTC
TTTATAGAATTCGTCAGGAAAGATCTTTTGAAAAATTGGATATTTACCAGTACGGCAAGGAAATGGCAAGTCTAGTGGAA
GGCCTCCGAAAAGCTCAAAGCTTATCTGTTGACATGGACCACATTGAAGCTTTAGATCAAGCGCTAGCCATTCATAACAA
ATACGATATCGTCCTTAATGGTTCTCACCTGATTAAGGATTTTGGGATGAAACCAGGTCCTCAGCTAGGGCTTATGCTTG
AGAAAGTTGAATTAGCGATTGTAGAGGGACGCCTAGATAATGATTTCACTACAATTGAAGCATTTGTTAGAGAGGAATTA
GCAACATGA

Upstream 100 bases:

>100_bases
TTGCAAGAATCACTAAAACAATAACAAAATGTGATAGAACTCAAGTAGCCTAATCACACTTTAGATTGGGCTAGTTGGAT
GATAGGTTAGAAAACAACAT

Downstream 100 bases:

>100_bases
GCCATTTTTTGGTGGAGAAATTGACAAAAACGGTGGGAGATAAAACAGTCTTTCAGGATATTTCCTTTATTATTCATGAC
TTTGACCGTATTGGGATTAT

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENG
GEYEITTFRTEDVYVDYRRPSQVSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLN
HLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVE
GLRKAQSLSVDMDHIEALDQALAIHNKYDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREEL
AT

Sequences:

>Translated_402_residues
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENG
GEYEITTFRTEDVYVDYRRPSQVSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLN
HLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVE
GLRKAQSLSVDMDHIEALDQALAIHNKYDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREEL
AT
>Mature_402_residues
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENG
GEYEITTFRTEDVYVDYRRPSQVSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLN
HLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVE
GLRKAQSLSVDMDHIEALDQALAIHNKYDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREEL
AT

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=224, Percent_Identity=36.1607142857143, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI87081691, Length=211, Percent_Identity=35.0710900473934, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789436, Length=195, Percent_Identity=38.4615384615385, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71995920, Length=234, Percent_Identity=35.042735042735, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI281360112, Length=283, Percent_Identity=32.8621908127208, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21357337, Length=283, Percent_Identity=32.8621908127208, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46069; Mature: 46069

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAI
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHH
FNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQVSFVRSLEEDLKRRDFTV
HHHHHEEEECCCEEEEEECCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCEE
NALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDALRIMRGFRFAASLDFDIEAA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHH
TFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCC
HLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQE
EEEEEEECCCEECCCHHHEEHHEEEHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDHIEALDQALAIHNKYDIVLNGSHLIKD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHH
FGMKPGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREELAT
CCCCCCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAI
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHH
FNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQVSFVRSLEEDLKRRDFTV
HHHHHEEEECCCEEEEEECCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCEE
NALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDALRIMRGFRFAASLDFDIEAA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHH
TFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCC
HLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQE
EEEEEEECCCEECCCHHHEEHHEEEHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDHIEALDQALAIHNKYDIVLNGSHLIKD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHH
FGMKPGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREELAT
CCCCCCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]