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The map label for this gene is thiI [H]

Identifier: 21910086

GI number: 21910086

Start: 604910

End: 606064

Strand: Direct

Name: thiI [H]

Synonym: SpyM3_0550

Alternate gene names: 21910086

Gene position: 604910-606064 (Clockwise)

Preceding gene: 21910085

Following gene: 21910087

Centisome position: 31.83

GC content: 41.82

Gene sequence:

>1155_bases
ATGCGATTCATTAACAAACTTAAAAACAATATTCAAGACGTACTGGCTCCTTTCCCAGCCATTACTGTCCGTTCAGATCG
TGATAGGACCCATGTGTCTCTAAATGGCACAGATTATCAGCCTATTGTAGAAGCTTTAAAGCTGGTGTTTGGTGTTCAAG
CTCTATCTCCTGTTTATAAACTTGAAAAAAGTGTCCCTCTTTTGGTAACAGCTGTTCAAGATATCATGACTTCTCTTTAT
CGTGATGGACTGACGTTTAAGATTGCCACTAAGCGTAGTGATCACGCGTTTGAATTGGATAGTCGTGAGCTTAACAGTCT
TCTTGGTGGAGCTGTTTTTGAGGTTTTACCAAATATCCAAGCCCAGATGAAACATCCCGATGTGACTCTCAAAGTCGAAA
TTCGTGATGAAGCAGCTTACATTTCTTATGAAGAGATTAAGGGAGCAGGCGGGTTACCAGTAGGAACGTCAGGGAAAGGG
ATGCTGATGTTATCGGGCGGTATTGATTCACCAGTAGCTGGGTATTTAGCCCTTAAACGTGGCCTTGATATTGAAGTTGT
TCATTTTGCTAGCCCTCCGTACACCAGTCCAGGTGCCTTGGCTAAAGCCCAAGACCTTACTCGTCGGTTAACTCGTTTTG
GTGGAAATATCCAATTTATCGAGGTTCCTTTTACAGAAATTCAAGAAGAAATTAAAAATAAGGCACCAGAGGCTTACCTT
ATGACATTAACCCGCCGCTTTATGATGCGCATCACAGATGCCATTCGTGAGCAACGTAAGGGTCTTGTTATTGTTAACGG
TGAGAGCCTAGGTCAGGTTGCTAGTCAAACCCTTGAAAGCATGCAGGCCATTAATGCGGTAACATCAACCCCAATTATTA
GACCAGTCGTGACAATGGATAAACTTGAAATTATCGAGATGGCTCAGGCGATTGATACTTTTGACATTTCTATTCAACCG
TTCGAAGATTGCTGTACTATTTTTGCACCAGACCGTCCTAAAACCAACCCTAAACTAGGTAATGCTGAAAAATATGAAGA
ACGCTTTGATATTGATGGTCTTGTCCAGCGTGCTGTGTCAGGAATTGTTGTCACAGAAATCACACCTGAAATCGTCAATG
ATGAGGTTGAAAATCTCATTGATGCTTTACTTTAA

Upstream 100 bases:

>100_bases
TTTATGATAAAACACAGAAAGTTAGGTAAGATTAGGTAAGATGGACTATTCAGAAATTATGGTTCGCCATGGTGAACTAT
CAACTAAAGGGAAGAATCGC

Downstream 100 bases:

>100_bases
AAGAAAAGATCAAGTGTAAATCTTTTAGTAGTCAGTAATGATACTATTGAAAAGCCTCTATAAGGGCTTTTTTGCTGCAA
TGAAGGAGGAAAATTTTGAG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLY
RDGLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAYISYEEIKGAGGLPVGTSGKG
MLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYL
MTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTFDISIQP
FEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIVNDEVENLIDALL

Sequences:

>Translated_384_residues
MRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLY
RDGLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAYISYEEIKGAGGLPVGTSGKG
MLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYL
MTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTFDISIQP
FEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIVNDEVENLIDALL
>Mature_384_residues
MRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLY
RDGLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAYISYEEIKGAGGLPVGTSGKG
MLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYL
MTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTFDISIQP
FEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIVNDEVENLIDALL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=374, Percent_Identity=28.8770053475936, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 42451; Mature: 42451

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYK
CCHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LEKSVPLLVTAVQDIMTSLYRDGLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQ
HHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECCHHHHHHHHHHHHHHHHCCCH
AQMKHPDVTLKVEIRDEAAYISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKR
HHCCCCCEEEEEEEECCCCEEEHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHC
GLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYL
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHHH
MTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMD
HHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KLEIIEMAQAIDTFDISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVS
HHHHHHHHHHHHHHCCEECCHHHHHEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHC
GIVVTEITPEIVNDEVENLIDALL
CEEEEECCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYK
CCHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LEKSVPLLVTAVQDIMTSLYRDGLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQ
HHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECCHHHHHHHHHHHHHHHHCCCH
AQMKHPDVTLKVEIRDEAAYISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKR
HHCCCCCEEEEEEEECCCCEEEHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHC
GLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYL
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHHH
MTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMD
HHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KLEIIEMAQAIDTFDISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVS
HHHHHHHHHHHHHHCCEECCHHHHHEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHC
GIVVTEITPEIVNDEVENLIDALL
CEEEEECCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]