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The map label for this gene is nifS [C]

Identifier: 21910085

GI number: 21910085

Start: 603696

End: 604838

Strand: Direct

Name: nifS [C]

Synonym: SpyM3_0549

Alternate gene names: 21910085

Gene position: 603696-604838 (Clockwise)

Preceding gene: 21910082

Following gene: 21910086

Centisome position: 31.76

GC content: 41.29

Gene sequence:

>1143_bases
ATGATTTATTTTGATAATGCAGCCACAACCATACCTTATGGAGAGGCTCTTAAAACTTACCAAGAAGTAGCAACAAAAAT
ATATGGCAATCCCTCAAGTCTGCACCAACTAGGGACAAATGCATCTCGCATTTTAGAAGCTTCCCGTAAACAGATTGCTG
GTTTGTTGGGTGTTAAGTCAGAAGAAATCTTTTTTACGTCTGGTGGGACTGAAAGTGCTAATTGGGCTATTAAGGGAATT
GCTTTTGAAAAAACCGCTTTTGGCAAACATATTATTATCTCAGCCATTGAACACCCTGCCGTGAGTGAAAGTGTCAAATG
GCTTCTCACACAAGGATTCGAAGTTTCTTATGCTCCAGTGACAACACAGGGAGTTGTTGATGTGAATGCTTTAGCTGAGC
TGATTAGACCAGACACCATCTTAATCTCGATTATGGCTGTTAATAATGAAATGGGAGCTATCCAACCGATTAGAGCCATT
TCAAACCTATTGGCTAACCAACTAACGATCACCTTTCACGTTGATGCTGTTCAAGCCATTGGTAAAATACCACTCTGTGA
TTATATGACTAATCGTGTGGATTTGGCTTCTTTTTCAGGGCACAAATTTCATAGTGTTCGTGGTGTTGGCTTCCTTTATA
AAAAAGCAGGCAAGCGACTCAATCCTCTCTTGTCAGGCGGCGGCCAAGAGCAGGAGTTACGTTCAACCACTGAAAATGTT
GCAGGAATTGCTAGCATGGCCAAAGCATTAAGAATAGTCACTGAAAAACAAGTGAGCGTCTTACCTAAACTAACAGCGAT
GCGAGATGTGATTTACAAAGCTTTGTCCGCTTACCCAGATGTGACAGTATTTTCAGCTCAAGAAGGCTTTGTGCCCAATA
TTTTGACCTTTGGGATTAGAGGGGTTCGAGGAGAAGTTATTGTCCACGCCTTTGAAAAATATGAGATTTATATTTCGACC
ACAAGTGCATGTTCGTCCAAAGCAGGTAAGCCAGCAGGAAGCCTAGTGGCTATGGGGATTCCTGTAAAAACTGCCCAGAC
AGCCGTTCGTATTAGTCTAGACGATGACAACGATATGGGACAAGTAGAGCAATTTTTGACCATATTCAAACAGATTTATG
ATAAAACACAGAAAGTTAGGTAA

Upstream 100 bases:

>100_bases
ACAAGGAAGTCCTTTGGGCTTATTGTAATACGAATATTCTTGATTTCATTTTAGCTATTTTGTGATAGAATAAAGTATTG
AGTAAAAATGAAGGTGACAC

Downstream 100 bases:

>100_bases
GATTAGGTAAGATGGACTATTCAGAAATTATGGTTCGCCATGGTGAACTATCAACTAAAGGGAAGAATCGCATGCGATTC
ATTAACAAACTTAAAAACAA

Product: putative iron-sulfur cofactor synthesis protein

Products: L-alanine; S [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRILEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGI
AFEKTAFGKHIIISAIEHPAVSESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
SNLLANQLTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGKRLNPLLSGGGQEQELRSTTENV
AGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALSAYPDVTVFSAQEGFVPNILTFGIRGVRGEVIVHAFEKYEIYIST
TSACSSKAGKPAGSLVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR

Sequences:

>Translated_380_residues
MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRILEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGI
AFEKTAFGKHIIISAIEHPAVSESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
SNLLANQLTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGKRLNPLLSGGGQEQELRSTTENV
AGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALSAYPDVTVFSAQEGFVPNILTFGIRGVRGEVIVHAFEKYEIYIST
TSACSSKAGKPAGSLVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR
>Mature_380_residues
MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRILEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGI
AFEKTAFGKHIIISAIEHPAVSESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
SNLLANQLTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGKRLNPLLSGGGQEQELRSTTENV
AGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALSAYPDVTVFSAQEGFVPNILTFGIRGVRGEVIVHAFEKYEIYIST
TSACSSKAGKPAGSLVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR

Specific function: Fe-S cluster biosynthesis [C]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=356, Percent_Identity=34.2696629213483, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI156713448, Length=407, Percent_Identity=30.958230958231, Blast_Score=159, Evalue=6e-39,
Organism=Escherichia coli, GI48994898, Length=357, Percent_Identity=37.8151260504202, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1789175, Length=350, Percent_Identity=26.8571428571429, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787970, Length=350, Percent_Identity=24.8571428571429, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=357, Percent_Identity=33.3333333333333, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17533177, Length=326, Percent_Identity=30.3680981595092, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=384, Percent_Identity=34.375, Blast_Score=206, Evalue=4e-54,
Organism=Drosophila melanogaster, GI20129463, Length=360, Percent_Identity=35.5555555555556, Blast_Score=192, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: NA

Molecular weight: Translated: 41225; Mature: 41225

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRILEASRKQIAGLLGVKS
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
EEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVSESVKWLLTQGFEVSYAPV
CEEEEECCCCCCCCEEEEEEEEEHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCC
TTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAISNLLANQLTITFHVDAVQAI
CCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEEHHHHHH
GKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGKRLNPLLSGGGQEQELRSTTENV
CCCCCHHHHCCCEEEECCCCCCEEEECCHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHH
AGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALSAYPDVTVFSAQEGFVPNILTFGIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCC
GVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGSLVAMGIPVKTAQTAVRISLDDDNDMG
CCCCCEEEEEEEEEEEEEEECCHHHCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCHH
QVEQFLTIFKQIYDKTQKVR
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRILEASRKQIAGLLGVKS
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
EEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVSESVKWLLTQGFEVSYAPV
CEEEEECCCCCCCCEEEEEEEEEHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCC
TTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAISNLLANQLTITFHVDAVQAI
CCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEEHHHHHH
GKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGKRLNPLLSGGGQEQELRSTTENV
CCCCCHHHHCCCEEEECCCCCCEEEECCHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHH
AGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALSAYPDVTVFSAQEGFVPNILTFGIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCC
GVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGSLVAMGIPVKTAQTAVRISLDDDNDMG
CCCCCEEEEEEEEEEEEEEECCHHHCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCHH
QVEQFLTIFKQIYDKTQKVR
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cysteine [C]

Specific reaction: L-cysteine = L-alanine + S [C]

General reaction: transfers sulfane sulfur to ThiI during in vitro biosynthesis of 4-thiouridine in E. coli tRNA [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8031904 [H]