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The map label for this gene is ligT [H]

Identifier: 218930412

GI number: 218930412

Start: 3793586

End: 3794161

Strand: Direct

Name: ligT [H]

Synonym: YPO3395

Alternate gene names: 218930412

Gene position: 3793586-3794161 (Clockwise)

Preceding gene: 218930406

Following gene: 218930413

Centisome position: 81.52

GC content: 56.6

Gene sequence:

>576_bases
ATGACAAATACCGTTGACGAATTAAAACGTGTCCGCCGCCTATTCTTTGCCCTGACACTGCCGGATGCCATGCAACAAGA
GATTGTGCAATGGCGAGCTGGTCATTTCTCGCCAGAGGCGGGTCGCCCTGTCGCGGCGGCGAATCTGCACCTGACACTGG
CGTTCCTTGGTGAAGTCAGTGCGACAAAATCCCAGGTGTTACAGCAGCAAGCCGGGCGTATCCAACAAGCAGGTTTCAGC
GTGACATTAGATGATATCGGCCATTGGCCCGGTTCCGGCGTGATTTGGCTGGGATGTAAGAACCCCCCTCGTGGCCTGTT
GCAATTGGCGCAGTTGCTGCGCTCCCAGGCAGCACGTAGTGGCTGTTATCAAACGCCGCTGCCGTTTCATCCCCATGTTA
CTCTGTTGCGTAACGCAACCCGCCCAGTGGCATTACCTGCCAAGAGCGGCAATGAGACCTTCCAGGCGGACCATTTCTCG
CTGTATGAATCGGTCTTCGCGCGGGGCCGCACCCGCTATAACATCGTACAAAGCTGGCCGCTGGCTGGCAGTGAAAGGAA
ACCAGATGCCTGCTAA

Upstream 100 bases:

>100_bases
CACACGCTCTACTTTTATCGTCAGCCATTGTAGCTTTATCGTCAGCCATTGTAGCATCATGACCGTAGAGCGTATTTAGC
CGAGTGATCATGCCAACCTA

Downstream 100 bases:

>100_bases
CGCCAACTTGCCACCCTTTAGCGCCAACTTACTGTCAGTGAACTCAGATTCGCGGTCAATTAGCCCTAATTCGCCACCGA
CTCACGCCAAATCGCTGCAA

Product: putative 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MTNTVDELKRVRRLFFALTLPDAMQQEIVQWRAGHFSPEAGRPVAAANLHLTLAFLGEVSATKSQVLQQQAGRIQQAGFS
VTLDDIGHWPGSGVIWLGCKNPPRGLLQLAQLLRSQAARSGCYQTPLPFHPHVTLLRNATRPVALPAKSGNETFQADHFS
LYESVFARGRTRYNIVQSWPLAGSERKPDAC

Sequences:

>Translated_191_residues
MTNTVDELKRVRRLFFALTLPDAMQQEIVQWRAGHFSPEAGRPVAAANLHLTLAFLGEVSATKSQVLQQQAGRIQQAGFS
VTLDDIGHWPGSGVIWLGCKNPPRGLLQLAQLLRSQAARSGCYQTPLPFHPHVTLLRNATRPVALPAKSGNETFQADHFS
LYESVFARGRTRYNIVQSWPLAGSERKPDAC
>Mature_190_residues
TNTVDELKRVRRLFFALTLPDAMQQEIVQWRAGHFSPEAGRPVAAANLHLTLAFLGEVSATKSQVLQQQAGRIQQAGFSV
TLDDIGHWPGSGVIWLGCKNPPRGLLQLAQLLRSQAARSGCYQTPLPFHPHVTLLRNATRPVALPAKSGNETFQADHFSL
YESVFARGRTRYNIVQSWPLAGSERKPDAC

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage [H]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=170, Percent_Identity=60, Blast_Score=216, Evalue=7e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 21112; Mature: 20981

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTVDELKRVRRLFFALTLPDAMQQEIVQWRAGHFSPEAGRPVAAANLHLTLAFLGEVS
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEHHHEEEEHHHHCHH
ATKSQVLQQQAGRIQQAGFSVTLDDIGHWPGSGVIWLGCKNPPRGLLQLAQLLRSQAARS
HHHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
GCYQTPLPFHPHVTLLRNATRPVALPAKSGNETFQADHFSLYESVFARGRTRYNIVQSWP
CCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEHHCCCC
LAGSERKPDAC
CCCCCCCCCCC
>Mature Secondary Structure 
TNTVDELKRVRRLFFALTLPDAMQQEIVQWRAGHFSPEAGRPVAAANLHLTLAFLGEVS
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEHHHEEEEHHHHCHH
ATKSQVLQQQAGRIQQAGFSVTLDDIGHWPGSGVIWLGCKNPPRGLLQLAQLLRSQAARS
HHHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
GCYQTPLPFHPHVTLLRNATRPVALPAKSGNETFQADHFSLYESVFARGRTRYNIVQSWP
CCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEHHCCCC
LAGSERKPDAC
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112 [H]