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The map label for this gene is ycjG [H]

Identifier: 218929433

GI number: 218929433

Start: 2635352

End: 2636326

Strand: Reverse

Name: ycjG [H]

Synonym: YPO2341

Alternate gene names: 218929433

Gene position: 2636326-2635352 (Counterclockwise)

Preceding gene: 218929436

Following gene: 218929431

Centisome position: 56.65

GC content: 52.1

Gene sequence:

>975_bases
ATGAGAACAGTGCGTTTTTACCCAGAGGCCTGGCCTCTTCACTCCGCGTTTGTCATTTCCCGAGGCAGCCGCACTGAAGC
TAAAGTTATCGTTGTTGAAATTGAGGAGAACGACATTCATGCATTCGGTGAATGTACTCCATACCCGCATTATGGAGAAA
GTGAATCTTCAGTGATGGCACAGCTTGCTTTGGTGGCTAATGCTATTGAGAATGGCGTCTCGCGGGAAGCCTTACAGCCA
CTTTTACCGGCCGGAGCGGCGAGGAATGCCGTCGATTCTGCGTTATGGCAACTGGAGTGTTTGCAACATCAGCAAAGTTT
ATGGCAACGAAGCCAGATAACGGCAGTTGAAACCGTTGCCATGGCTCAGACTGTCAGTATTGGTACTCCGGAAGCCATGG
CATGCAGTGCCAAAGCATTGGTGCAGCTTGGGGCCCACTTGCTGAAAGTCAAACTTGATGAGCACCTGATCGCCGAACGC
TTGGTTGCGATACGCAGTGCGGTGCCAGAGGTAACGTTAATCGTTGATGCTAATGAGGCTTGGCAACCTGAAGGGCTGGC
AGCGCGTTGCCAATTACTTGCTGACTTGGGGGTGGTGATGCTAGAACAGCCGCTACCCGCAGGAGAAGACAGTAGTTTAC
AGAATTTCATCCATCCATTACCCCTCTGTGCGGATGAAAGCTGTCACACGCGGGCTGATTTAGCGGGTTTGGTGGGTCGC
TATGACATGGTAAACATCAAACTGGATAAAAGTGGTGGGCTGACCGAGGCACTGCTACTGGCTGAACAGGCTAAATCGAT
GGGCTTTGCCATTATGCTCGGCTGTATGCTGTGTACCTCTCGTGCCATCCGTGCGGCACTGCCCTTAGCACCGGGGGCGC
GTTTTATTGATTTAGATGGCCCGACCTGGTTGGCAAATGACGTAGAGGGGGGACTGCATTTTTCTACTGGTACGATAGAC
CTACGGGTACGCTAG

Upstream 100 bases:

>100_bases
GATATAGCAAGTTCGCTAAAAAACCCGTTTAAAGACTTAACGGATAAACGTATGAGGGTCTCATACCTAAGCTAAAACTG
TATTCTGCCAAGGAGCGATA

Downstream 100 bases:

>100_bases
ACCTACTGGCACGCTAGACCTCGCGCGATAGATCTGTCGGTTTAATCCGCCAACGTCAGTAACTCAATGAAAGCAGCCAG
ATAGTATTCGCTGGCTGAAT

Product: putative mandelate racemase / muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MRTVRFYPEAWPLHSAFVISRGSRTEAKVIVVEIEENDIHAFGECTPYPHYGESESSVMAQLALVANAIENGVSREALQP
LLPAGAARNAVDSALWQLECLQHQQSLWQRSQITAVETVAMAQTVSIGTPEAMACSAKALVQLGAHLLKVKLDEHLIAER
LVAIRSAVPEVTLIVDANEAWQPEGLAARCQLLADLGVVMLEQPLPAGEDSSLQNFIHPLPLCADESCHTRADLAGLVGR
YDMVNIKLDKSGGLTEALLLAEQAKSMGFAIMLGCMLCTSRAIRAALPLAPGARFIDLDGPTWLANDVEGGLHFSTGTID
LRVR

Sequences:

>Translated_324_residues
MRTVRFYPEAWPLHSAFVISRGSRTEAKVIVVEIEENDIHAFGECTPYPHYGESESSVMAQLALVANAIENGVSREALQP
LLPAGAARNAVDSALWQLECLQHQQSLWQRSQITAVETVAMAQTVSIGTPEAMACSAKALVQLGAHLLKVKLDEHLIAER
LVAIRSAVPEVTLIVDANEAWQPEGLAARCQLLADLGVVMLEQPLPAGEDSSLQNFIHPLPLCADESCHTRADLAGLVGR
YDMVNIKLDKSGGLTEALLLAEQAKSMGFAIMLGCMLCTSRAIRAALPLAPGARFIDLDGPTWLANDVEGGLHFSTGTID
LRVR
>Mature_324_residues
MRTVRFYPEAWPLHSAFVISRGSRTEAKVIVVEIEENDIHAFGECTPYPHYGESESSVMAQLALVANAIENGVSREALQP
LLPAGAARNAVDSALWQLECLQHQQSLWQRSQITAVETVAMAQTVSIGTPEAMACSAKALVQLGAHLLKVKLDEHLIAER
LVAIRSAVPEVTLIVDANEAWQPEGLAARCQLLADLGVVMLEQPLPAGEDSSLQNFIHPLPLCADESCHTRADLAGLVGR
YDMVNIKLDKSGGLTEALLLAEQAKSMGFAIMLGCMLCTSRAIRAALPLAPGARFIDLDGPTWLANDVEGGLHFSTGTID
LRVR

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=321, Percent_Identity=67.9127725856698, Blast_Score=432, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 34949; Mature: 34949

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTVRFYPEAWPLHSAFVISRGSRTEAKVIVVEIEENDIHAFGECTPYPHYGESESSVMA
CCEEEECCCCCCCCEEEEEECCCCCCEEEEEEEEECCCCEECCCCCCCCCCCCCHHHHHH
QLALVANAIENGVSREALQPLLPAGAARNAVDSALWQLECLQHQQSLWQRSQITAVETVA
HHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
MAQTVSIGTPEAMACSAKALVQLGAHLLKVKLDEHLIAERLVAIRSAVPEVTLIVDANEA
HHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
WQPEGLAARCQLLADLGVVMLEQPLPAGEDSSLQNFIHPLPLCADESCHTRADLAGLVGR
CCCCCHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCC
YDMVNIKLDKSGGLTEALLLAEQAKSMGFAIMLGCMLCTSRAIRAALPLAPGARFIDLDG
CEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
PTWLANDVEGGLHFSTGTIDLRVR
CEEECCCCCCCEEEECCEEEEEEC
>Mature Secondary Structure
MRTVRFYPEAWPLHSAFVISRGSRTEAKVIVVEIEENDIHAFGECTPYPHYGESESSVMA
CCEEEECCCCCCCCEEEEEECCCCCCEEEEEEEEECCCCEECCCCCCCCCCCCCHHHHHH
QLALVANAIENGVSREALQPLLPAGAARNAVDSALWQLECLQHQQSLWQRSQITAVETVA
HHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
MAQTVSIGTPEAMACSAKALVQLGAHLLKVKLDEHLIAERLVAIRSAVPEVTLIVDANEA
HHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
WQPEGLAARCQLLADLGVVMLEQPLPAGEDSSLQNFIHPLPLCADESCHTRADLAGLVGR
CCCCCHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCC
YDMVNIKLDKSGGLTEALLLAEQAKSMGFAIMLGCMLCTSRAIRAALPLAPGARFIDLDG
CEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
PTWLANDVEGGLHFSTGTIDLRVR
CEEECCCCCCCEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]