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The map label for this gene is focA [H]

Identifier: 218928532

GI number: 218928532

Start: 1560393

End: 1561250

Strand: Reverse

Name: focA [H]

Synonym: YPO1384

Alternate gene names: 218928532

Gene position: 1561250-1560393 (Counterclockwise)

Preceding gene: 218928533

Following gene: 218928531

Centisome position: 33.55

GC content: 45.22

Gene sequence:

>858_bases
GTGAAAGCTGACAACCCCTTCGATGCATTATTACCTTCGGCAATGGCTAAAGTAGCTGAAGATGCTGGTGTCTATAAAGC
CACCAAGCATCCGATGACAACTTTTTATTTAGCGATTACCGCGGGTGTCTTTATTTCCATTGCGTTTGTTTTTTATATTA
CAGCAACCACCGGTACTGCAGGCGTTCCTTTTGGTTTTGCCAAACTGGTCGGTGGTATTTGTTTCTCCTTAGGGCTAATG
TTAGTCGTGGTGTGTGGGGGTGATCTTTTCACTTCAACTGTGCTTACCACGGTTGCCAAAGCCAGTGGCCGTATCACCTG
GCGTCAATTGGCAACTAACTGGGTTAATGTCTATATTGGGAACTTGTTCGGCGCACTGTTCTTCGTTGGCCTTATCTGGT
TTGCAGGCCAATATACTGTGGCAAATGGTCAATGGGGTTTAAATGTTCTGCAAACAGCAGAGCACAAATTGCACCATACT
TTTATTGAGGCAGTGTGCTTAGGTATTTTGGCTAACCTGATGGTTTGCCTGGCGGTTTGGATGAGTTACTCCGGCCGTAC
CATAACAGACAAAATGTTAGCGATGATTTTACCGGTGGGAATGTTTGTTGCCAGCGGTTTTGAGCATAGCATCGCCAATA
TGTTTATGATCCCGTTGGGTATTGTGATTAAAAACTTTGCTTCACCTGAGTTTTGGCAAGCCATCGGTTCAGCACCCGAG
CAATTCGCTCACTTGACCGTAAGTAACTTTATTATCGATAACCTGATCCCAGTCACCATCGGCAACATCATTGGTGGCGG
ACTATTGGTAGGGTTGACTTATTGGGTAATTTATCTGCGCGGTGACCAGCAACATTAA

Upstream 100 bases:

>100_bases
CTAAACTAAACTCGCCCAATATGATCCATATCAATTTTCACGCTATACTGCTTTGCTAGTATCTCACTGTGCTTTATTAA
CCCTATAAGAGAGAGTTAGT

Downstream 100 bases:

>100_bases
CGTGTGGCCGCTACGTCTGGTTTTCGAGAAATTAACATTAAAAGGTAGATGCATTATGACCGAACTTAATGAGAAATTGG
CCAAAGCATGGCACGGTTTT

Product: formate transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKADNPFDALLPSAMAKVAEDAGVYKATKHPMTTFYLAITAGVFISIAFVFYITATTGTAGVPFGFAKLVGGICFSLGLM
LVVVCGGDLFTSTVLTTVAKASGRITWRQLATNWVNVYIGNLFGALFFVGLIWFAGQYTVANGQWGLNVLQTAEHKLHHT
FIEAVCLGILANLMVCLAVWMSYSGRTITDKMLAMILPVGMFVASGFEHSIANMFMIPLGIVIKNFASPEFWQAIGSAPE
QFAHLTVSNFIIDNLIPVTIGNIIGGGLLVGLTYWVIYLRGDQQH

Sequences:

>Translated_285_residues
MKADNPFDALLPSAMAKVAEDAGVYKATKHPMTTFYLAITAGVFISIAFVFYITATTGTAGVPFGFAKLVGGICFSLGLM
LVVVCGGDLFTSTVLTTVAKASGRITWRQLATNWVNVYIGNLFGALFFVGLIWFAGQYTVANGQWGLNVLQTAEHKLHHT
FIEAVCLGILANLMVCLAVWMSYSGRTITDKMLAMILPVGMFVASGFEHSIANMFMIPLGIVIKNFASPEFWQAIGSAPE
QFAHLTVSNFIIDNLIPVTIGNIIGGGLLVGLTYWVIYLRGDQQH
>Mature_285_residues
MKADNPFDALLPSAMAKVAEDAGVYKATKHPMTTFYLAITAGVFISIAFVFYITATTGTAGVPFGFAKLVGGICFSLGLM
LVVVCGGDLFTSTVLTTVAKASGRITWRQLATNWVNVYIGNLFGALFFVGLIWFAGQYTVANGQWGLNVLQTAEHKLHHT
FIEAVCLGILANLMVCLAVWMSYSGRTITDKMLAMILPVGMFVASGFEHSIANMFMIPLGIVIKNFASPEFWQAIGSAPE
QFAHLTVSNFIIDNLIPVTIGNIIGGGLLVGLTYWVIYLRGDQQH

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=285, Percent_Identity=82.4561403508772, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI1788837, Length=282, Percent_Identity=49.290780141844, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI87082249, Length=225, Percent_Identity=28.8888888888889, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321779, Length=205, Percent_Identity=29.2682926829268, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 30792; Mature: 30792

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]