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The map label for this gene is trmL

Identifier: 218927290

GI number: 218927290

Start: 80447

End: 80935

Strand: Reverse

Name: trmL

Synonym: YPO0071

Alternate gene names: 218927290

Gene position: 80935-80447 (Counterclockwise)

Preceding gene: 218927292

Following gene: 218927283

Centisome position: 1.74

GC content: 49.08

Gene sequence:

>489_bases
ATGCTTAACATTGTTTTATTTGAACCCGAAATTCCGCCAAATACCGGCAATATTATCCGGTTATGTGCGAATACCGGCTG
CCAGCTTCATCTGATTAAACCTTTGGGTTTTACTTGGGACGATAAACGTTTACGCCGTGCGGGCCTTGATTACCATGAGT
TTGCCGACATCAAACATCACCATGATTATCAAGCCTTCCTGGACAGTGAAAAACTCGATAGCACACAACCGGCTCGCTTA
TTTGCGCTGACGACCAAGGGGACGCCGGCTCACAGTGCGGTGAGCTATCAGGCGAATGATTACCTGTTATTTGGCCCAGA
AACCCGTGGCTTACCGGCCTATATTCTGGATGCGTTACCCGCTCAGCAGAAAATCAGAATCCCCATGCAAGCCGATAGCC
GGAGCATGAATCTATCGAATGCCGTTTCAGTCGTGGTGTATGAGGCCTGGCGTCAACTGGGCTATCCAGGTGCATTACTG
AAAGAGTGA

Upstream 100 bases:

>100_bases
CTCCTTGCTTTTAAATTTTTGTTTTACACCGCCGCTACTGGCAACCGCTGCACTGAACAAGGGTTTCTGGTATCCTGCGC
CCCTCATTATTGGGGGACAT

Downstream 100 bases:

>100_bases
TTTTTCTTACGCAGAGAGAACGTGTCAGCCACAATCAGAGTGGCTAATCTGCCCCATATTAGGGGCATATCCAAAACAGT
TGGCGCTGGCGTTGCAACAA

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MLNIVLFEPEIPPNTGNIIRLCANTGCQLHLIKPLGFTWDDKRLRRAGLDYHEFADIKHHHDYQAFLDSEKLDSTQPARL
FALTTKGTPAHSAVSYQANDYLLFGPETRGLPAYILDALPAQQKIRIPMQADSRSMNLSNAVSVVVYEAWRQLGYPGALL
KE

Sequences:

>Translated_162_residues
MLNIVLFEPEIPPNTGNIIRLCANTGCQLHLIKPLGFTWDDKRLRRAGLDYHEFADIKHHHDYQAFLDSEKLDSTQPARL
FALTTKGTPAHSAVSYQANDYLLFGPETRGLPAYILDALPAQQKIRIPMQADSRSMNLSNAVSVVVYEAWRQLGYPGALL
KE
>Mature_162_residues
MLNIVLFEPEIPPNTGNIIRLCANTGCQLHLIKPLGFTWDDKRLRRAGLDYHEFADIKHHHDYQAFLDSEKLDSTQPARL
FALTTKGTPAHSAVSYQANDYLLFGPETRGLPAYILDALPAQQKIRIPMQADSRSMNLSNAVSVVVYEAWRQLGYPGALL
KE

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily

Homologues:

Organism=Escherichia coli, GI1790034, Length=162, Percent_Identity=80.2469135802469, Blast_Score=275, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRML_YERPE (Q74Y93)

Other databases:

- EMBL:   AL590842
- EMBL:   AE017042
- PIR:   AG0009
- RefSeq:   NP_991474.1
- RefSeq:   YP_002345165.1
- PDB:   3N4J
- PDB:   3N4K
- PDBsum:   3N4J
- PDBsum:   3N4K
- ProteinModelPortal:   Q74Y93
- GeneID:   1172920
- GeneID:   2767318
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO0071
- KEGG:   ypm:YP_0071
- HOGENOM:   HBG309728
- OMA:   EAWRQNG
- ProtClustDB:   CLSK888396
- GO:   GO:0005737
- HAMAP:   MF_01885
- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537
- PIRSF:   PIRSF029256
- TIGRFAMs:   TIGR00185

Pfam domain/function: PF00588 SpoU_methylase

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18234; Mature: 18234

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: BINDING 83-83 BINDING 105-105 BINDING 127-127 BINDING 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNIVLFEPEIPPNTGNIIRLCANTGCQLHLIKPLGFTWDDKRLRRAGLDYHEFADIKHH
CEEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHCCCCC
HDYQAFLDSEKLDSTQPARLFALTTKGTPAHSAVSYQANDYLLFGPETRGLPAYILDALP
CCHHHHHCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCEEEECCCCCCCHHHHHHCCC
AQQKIRIPMQADSRSMNLSNAVSVVVYEAWRQLGYPGALLKE
CCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MLNIVLFEPEIPPNTGNIIRLCANTGCQLHLIKPLGFTWDDKRLRRAGLDYHEFADIKHH
CEEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHCCCCC
HDYQAFLDSEKLDSTQPARLFALTTKGTPAHSAVSYQANDYLLFGPETRGLPAYILDALP
CCHHHHHCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCEEEECCCCCCCHHHHHHCCC
AQQKIRIPMQADSRSMNLSNAVSVVVYEAWRQLGYPGALLKE
CCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360