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The map label for this gene is yvoF [H]

Identifier: 218906332

GI number: 218906332

Start: 4942572

End: 4943084

Strand: Reverse

Name: yvoF [H]

Synonym: BCAH820_5245

Alternate gene names: 218906332

Gene position: 4943084-4942572 (Counterclockwise)

Preceding gene: 218906333

Following gene: 218906331

Centisome position: 93.22

GC content: 40.55

Gene sequence:

>513_bases
GTGCGACGGACAACGCGCTATCCTGTTTCAGGAGAAAATTCACTATGGAATGTGTATAAAACAGTGTCTTTTTGGAAGGT
AATGAAGAATTTTATTATTATCCAAATTGCGCGTTACACACCATTTTTATCCGTCAAAAATTGGTTGTACCGCACATTTT
TGCGGATGAAAGTAGGAAAGAAAACATCGTTTGCGCTTATGGTAATGCCGGATATTATGTTCCCAGAAAAGATAACTGTG
GGAGACAATTCGATTATTGGTTATAATACGACGCTTTTAGCGCACGAATATTTAATTCGCGAATATCGACTTGGAGAGAT
CGTTATTGGAAACGAAGTGATGATCGGAGCAAATACGACTATATTACCAGGTGTGAGAATTGGGGATGGCGCGATTGTTT
CAGCTGGCACACTTGTTCATAAAGATGTACCGAGCGGTGCTTTCGTGGGTGGGAATCCAATGCGTGTTATTTATACGAAA
GAGCAAATGGAGGCTAGAGAAGGTTCATACTAG

Upstream 100 bases:

>100_bases
AAGCTTACAAGCCAGACTTTATGCTAGATAAAATGAGTGATTTATTGCCGATTTTGTCCGACATGAATCGTTCGTAAAGA
TAAGTAGAGGAGCGATTTAA

Downstream 100 bases:

>100_bases
AAATGTAACATTTCTCTTCTTTTTTAATGAATAAAAAGGAAATAATTGAGTATAATATACTGTTTTGTTTTTTACGGAAT
CGTTTATACTTATAGATAGA

Product: transferase hexapeptide domain protein

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MRRTTRYPVSGENSLWNVYKTVSFWKVMKNFIIIQIARYTPFLSVKNWLYRTFLRMKVGKKTSFALMVMPDIMFPEKITV
GDNSIIGYNTTLLAHEYLIREYRLGEIVIGNEVMIGANTTILPGVRIGDGAIVSAGTLVHKDVPSGAFVGGNPMRVIYTK
EQMEAREGSY

Sequences:

>Translated_170_residues
MRRTTRYPVSGENSLWNVYKTVSFWKVMKNFIIIQIARYTPFLSVKNWLYRTFLRMKVGKKTSFALMVMPDIMFPEKITV
GDNSIIGYNTTLLAHEYLIREYRLGEIVIGNEVMIGANTTILPGVRIGDGAIVSAGTLVHKDVPSGAFVGGNPMRVIYTK
EQMEAREGSY
>Mature_170_residues
MRRTTRYPVSGENSLWNVYKTVSFWKVMKNFIIIQIARYTPFLSVKNWLYRTFLRMKVGKKTSFALMVMPDIMFPEKITV
GDNSIIGYNTTLLAHEYLIREYRLGEIVIGNEVMIGANTTILPGVRIGDGAIVSAGTLVHKDVPSGAFVGGNPMRVIYTK
EQMEAREGSY

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=116, Percent_Identity=35.3448275862069, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 19280; Mature: 19280

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTTRYPVSGENSLWNVYKTVSFWKVMKNFIIIQIARYTPFLSVKNWLYRTFLRMKVGK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
KTSFALMVMPDIMFPEKITVGDNSIIGYNTTLLAHEYLIREYRLGEIVIGNEVMIGANTT
CCCEEEEEECCCCCCCEEEECCCCEEECCHHHHHHHHHHHHHCCCEEEECCEEEECCCCE
ILPGVRIGDGAIVSAGTLVHKDVPSGAFVGGNPMRVIYTKEQMEAREGSY
EECCEEECCCCEEECCEEEECCCCCCCEECCCCEEEEEEHHHHHHCCCCC
>Mature Secondary Structure
MRRTTRYPVSGENSLWNVYKTVSFWKVMKNFIIIQIARYTPFLSVKNWLYRTFLRMKVGK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
KTSFALMVMPDIMFPEKITVGDNSIIGYNTTLLAHEYLIREYRLGEIVIGNEVMIGANTT
CCCEEEEEECCCCCCCEEEECCCCEEECCHHHHHHHHHHHHHCCCEEEECCEEEECCCCE
ILPGVRIGDGAIVSAGTLVHKDVPSGAFVGGNPMRVIYTKEQMEAREGSY
EECCEEECCCCEEECCEEEECCCCCCCEECCCCEEEEEEHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]