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The map label for this gene is vacB [H]

Identifier: 218906302

GI number: 218906302

Start: 4914601

End: 4917021

Strand: Reverse

Name: vacB [H]

Synonym: BCAH820_5214

Alternate gene names: 218906302

Gene position: 4917021-4914601 (Counterclockwise)

Preceding gene: 218906303

Following gene: 218906300

Centisome position: 92.73

GC content: 41.31

Gene sequence:

>2421_bases
TTGGAAGAAATCATACAAGAACATATTGATAAGTTGTTATTATTTATGAGAGAAGAAGCGTATAAACCGCTAACGATACA
AGAGTTAGAAGAGGCATTTGGGATTGAAGGTTCTGAGGGCTTCAAAGATTTCGTAAAGGCACTTGTAACGATGGAAGAAA
AGGGACTTGTTATTCGTACTCGTAGCAACCGTTACGGTCTTCCTGAAAAGATGAATTTAGTACGTGGTAAGTTAATTGGA
CATGCACGTGGCTTTGCATTCGTTGTACCAGACGAGAAGAAAACAGGAGATGATGATCTTTTCATCCCGCCTACAGAGTT
AAACGGTGCACTTCATGGCGATACAGTATTAGCACGCCTTAGTTCCCAATCGAGCGGTTCGCGTCAAGAAGGTTCCATTG
TACGCATTTTAGAGCGTGGCACGAAAGAATTAGTTGGTACATATACAGAATCGAAAAACTTTGGATTTGTTATACCTGAC
AATAAACGTTGGACAAGTGACATTTTCGTATTGAAAAGCGCATCAATGGGCGCTGTAGAAGGTCATAAAGTAGTTGTGAA
AATTACGAGCTATCCAGAGAATCGTTTAAGTGCAGAAGGTGAAGTTATTCAAATTCTAGGTCATAAAAATGATCCAGGAG
TAGATATTTTATCTGTCATTCATAAACATCACTTGCCTTTAGCGTTCCCTGAAGAAGTGATGGAACATGCAAACAGTGTA
CCAGAAACGATTTCAGAAGAAGATTTAAAAGATCGCCGTGACCTACGTGACCAAATGATCGTAACAATTGACGGTGCAGA
CGCGAAAGATTTAGATGATGCTGTTACAGTAACGAAGCTCGAGAACGGTAACTATAAGCTTGGCGTTCACATTGCGGATG
TAAGCCATTACGTTCAAGAAGGTTCTCCAATTGATGTAGAAGCAGCGGAAAGAGCGACGAGTGTGTATCTTGTTGACCGT
GTAATTCCGATGATTCCACATCGTCTATCTAACGGTATTTGTTCATTAAATCCGAAAGTAGACCGTCTGACGTTATCTTG
TGAAATGGAAATCAACAACTTAGGTGACGTTGTCAAACACGAGATTTTCCAAAGTGTAATTAAAACGACAGAGCGTATGA
CGTATGCTGACGTAAGAAGCATTTTAGAAGATGAGGACGAAGAGCTAATTAAGCGTTATGAACCGTTAGTGCCGATGTTT
AAAGAGATGGGGCAATTAGCACAAATTTTACGTGAAAAACGTATGCGCCGCGGTGCAATCGACTTTGACTTTAAAGAAGC
GAAAGTATTAGTGGATGAAGAAGGAAAACCGACAGATGTTGTTATGCGTGATCGTTCTGTATCAGAGAAGTTAATTGAAG
AATTTATGCTTGTTGCGAACGAAACAGTAGCAGAGCACTTCCACTGGATGAACGTACCATTCATGTATCGTGTCCATGAA
GATCCGAAAGAAGATAAGTTAGAGCGTTTCTTCGAGTTTGTAACGAACTTCGGATATGCGGTAAAAGGACGTGCGAATGA
AGTGCATCCTCGTGCGCTGCAACAAATTCTTGAAATGGTTCAAGGACAGCCTGAAGAAGTAGTCATTTCAACAGTTATGC
TTCGCTCAATGAAGCAAGCACGTTACGATGCAGATAGCTTAGGACATTTCGGTTTATCAACGGAGTTCTACACGCATTTC
ACATCACCAATTCGTCGTTATCCAGATACGATTGTTCATAGATTAATTCGTGAATACATCATTAACGGTAAAGTCGACAA
TGAAACGCAAGCAAAATGGCGTGAAAAATTACCTGAGATTGCAGAACACTCTTCTAATATGGAACGTCGTGCTGTTGAAG
CAGAGCGTGAAACAGACGAAATGAAAAAAGCAGAGTATATGGTTGATAAGATCGGCGAAGAGTATGACGGTATGATTAGC
TCTGTAACAAACTTCGGTTTATTCGTAGAGCTTCCAAATACAATTGAAGGTCTTGTACACGTTAGCTACTTAACGGATGA
TTACTATCGTTATGATGAGCAGCACTTCGCAATGATCGGCGAACGTACAGGTAACGTATTCCGCATTGGTGACGAAATTA
CAATTCGCGTTATTAACGTAAACAAAGACGAGCGTGCAATTGACTTTGAAATCGTTGGTATGAAAGGTACGCCTCGTCGT
AAGTTCAAAGACCGTCCAGTCGTTATCGAACAGCCAAGAACAGGCAGAAAGAAACGCGGTGGACGTAGTGAGCGTAGTAA
TGAGCGCGGCGGAGAACGTGGCACAGGAAGAAAATTTGATCGTGGTGGCAAAGGGAAAGGAAGAGGATCTGCATCCGCAT
CCGCTAGCCAGCCAGGGAAAAAAGATGGTAACGGTAAGAAGAAAAAAGCATTCTTCGAAAACGTACCAGGATTCAAGAAG
AAAAAGAAAAAGCGTAAGTAA

Upstream 100 bases:

>100_bases
ACTGTAAAAATCATAAGGGGAGTATTGAAGTTTCACTTTATACATCCCCTATCTTTCGGATACACTAAATAATATAAGGT
TTTAAAGGAGGTATTTTTGC

Downstream 100 bases:

>100_bases
GAAAAGGATGGCTTCGCTTTGATAAAAAGCGAGGCTTTTTCTTTTTTATTGGTGGCTTGAGAGCGAGCTACTTTCCTCTT
TTGTTGGAATCCAGCTACAG

Product: ribonuclease R

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 806; Mature: 806

Protein sequence:

>806_residues
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRTRSNRYGLPEKMNLVRGKLIG
HARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARLSSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPD
NKRWTSDIFVLKSASMGAVEGHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQEGSPIDVEAAERATSVYLVDR
VIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKHEIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMF
KEMGQLAQILREKRMRRGAIDFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQARYDADSLGHFGLSTEFYTHF
TSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEIAEHSSNMERRAVEAERETDEMKKAEYMVDKIGEEYDGMIS
SVTNFGLFVELPNTIEGLVHVSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASASQPGKKDGNGKKKKAFFENVPGFKK
KKKKRK

Sequences:

>Translated_806_residues
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRTRSNRYGLPEKMNLVRGKLIG
HARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARLSSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPD
NKRWTSDIFVLKSASMGAVEGHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQEGSPIDVEAAERATSVYLVDR
VIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKHEIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMF
KEMGQLAQILREKRMRRGAIDFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQARYDADSLGHFGLSTEFYTHF
TSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEIAEHSSNMERRAVEAERETDEMKKAEYMVDKIGEEYDGMIS
SVTNFGLFVELPNTIEGLVHVSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASASQPGKKDGNGKKKKAFFENVPGFKK
KKKKRK
>Mature_806_residues
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRTRSNRYGLPEKMNLVRGKLIG
HARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARLSSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPD
NKRWTSDIFVLKSASMGAVEGHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQEGSPIDVEAAERATSVYLVDR
VIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKHEIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMF
KEMGQLAQILREKRMRRGAIDFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQARYDADSLGHFGLSTEFYTHF
TSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEIAEHSSNMERRAVEAERETDEMKKAEYMVDKIGEEYDGMIS
SVTNFGLFVELPNTIEGLVHVSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASASQPGKKDGNGKKKKAFFENVPGFKK
KKKKRK

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014625, Length=462, Percent_Identity=30.952380952381, Blast_Score=227, Evalue=4e-59,
Organism=Homo sapiens, GI190014623, Length=462, Percent_Identity=30.952380952381, Blast_Score=227, Evalue=4e-59,
Organism=Homo sapiens, GI134288890, Length=414, Percent_Identity=34.0579710144928, Blast_Score=217, Evalue=3e-56,
Organism=Homo sapiens, GI19115966, Length=575, Percent_Identity=28, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI219521928, Length=575, Percent_Identity=28, Blast_Score=191, Evalue=3e-48,
Organism=Escherichia coli, GI87082383, Length=725, Percent_Identity=38.7586206896552, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1787542, Length=639, Percent_Identity=24.8826291079812, Blast_Score=148, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17553506, Length=538, Percent_Identity=30.1115241635688, Blast_Score=205, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI212645896, Length=492, Percent_Identity=28.6585365853659, Blast_Score=189, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6324552, Length=589, Percent_Identity=28.0135823429542, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6320499, Length=422, Percent_Identity=25.5924170616114, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6323943, Length=432, Percent_Identity=22.9166666666667, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24649634, Length=475, Percent_Identity=31.1578947368421, Blast_Score=235, Evalue=9e-62,
Organism=Drosophila melanogaster, GI19922976, Length=363, Percent_Identity=32.5068870523416, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24654597, Length=363, Percent_Identity=32.5068870523416, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24654592, Length=363, Percent_Identity=32.5068870523416, Blast_Score=184, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 91875; Mature: 91875

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRT
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEE
RSNRYGLPEKMNLVRGKLIGHARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARL
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCHHCCCCCCHHHHHHHH
SSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPDNKRWTSDIFVLKSASMGAVE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCCCC
GHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
CCEEEEEEECCCCHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCC
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQE
CHHCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHHHC
GSPIDVEAAERATSVYLVDRVIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEHHHHHHHHHH
EIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMFKEMGQLAQILREKRMRRGAI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
CCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQA
CCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RYDADSLGHFGLSTEFYTHFTSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEI
HCCHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AEHSSNMERRAVEAERETDEMKKAEYMVDKIGEEYDGMISSVTNFGLFVELPNTIEGLVH
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEECCHHHHHHEE
VSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
EHHHHHHHHHHCCHHHHHHHCCCCCEEEECCEEEEEEEECCCCCCEEEEEEEECCCCCCC
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASASQPGK
CCCCCCEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KDGNGKKKKAFFENVPGFKKKKKKRK
CCCCCCHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRT
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEE
RSNRYGLPEKMNLVRGKLIGHARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARL
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCHHCCCCCCHHHHHHHH
SSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPDNKRWTSDIFVLKSASMGAVE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCCCC
GHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
CCEEEEEEECCCCHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCC
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQE
CHHCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHHHC
GSPIDVEAAERATSVYLVDRVIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEHHHHHHHHHH
EIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMFKEMGQLAQILREKRMRRGAI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
CCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQA
CCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RYDADSLGHFGLSTEFYTHFTSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEI
HCCHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AEHSSNMERRAVEAERETDEMKKAEYMVDKIGEEYDGMISSVTNFGLFVELPNTIEGLVH
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEECCHHHHHHEE
VSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
EHHHHHHHHHHCCHHHHHHHCCCCCEEEECCEEEEEEEECCCCCCEEEEEEEECCCCCCC
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASASQPGK
CCCCCCEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KDGNGKKKKAFFENVPGFKKKKKKRK
CCCCCCHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]